AYURVEDIC REVIEW ON DIAGNOSIS AND MANAGEMENT OF HRIDROGA WITH THE SPECIAL REFERENCE TO CARDIOVASCULAR DISEASE

According to AcharyaSushruta, in the presence of the Etiological factors the dosha get vitiated and provoked all the three Doshas spread out of their place and vitiate the RasaDhatu in the heart. Vitiate Rasa Dhatu (body lymph /chyle) manifestation of various types of pain is being produced, which is called ‘Hridbadha’ or Hridroga. Cardiovascular disease (CVD) is the most important cause of global death, accounting for 17.3 million deaths per year, a number that is expected to grow to more than 23.6 million by 2030. Aim and objectives-Diagnosis and treatment of Hridroga through Ayurveda and its modern correlation.Mode ofAction of Hridyadrugs promoting heart’s health.Improper diet (excessive intake of Kshar, Lavana Rasa, Virudahbhojana) and Vegadharna, Chinta,Krodhaetc. are few among the many causes of Hridroga. In understanding symptomology of cardiovasculardisorder, it should be noted thatVaivarnya (Panduta /Shweta/Shyava) can be correlated to pallor and cyanosis, Murcchato Syncope, Kasato cough with or without Hemoptysis, Shwasato breathlessness or dyspnea, Ruja to Chest pain or discomfort. Drugs used in various formulations in Hridrogahaveproperties like Pachana, Deepana, Hridya, Anulomana,Rasayana and Krimihara.So, in present article an effort has been made to explain the heart disease and its management through Ayurveda as well as modern medicine

Ciprofloxacin-Resistant Neisseria meningitidis, Delhi, India

Decreased susceptibility of Neisseria meningitidis isolates to ciprofloxacin emerged from an outbreak in Delhi, India. Results of antimicrobial susceptibility testing of the meningococcal isolates to ciprofloxacin and further sequencing of DNA gyrase A quinolone-resistance–determining region confirmed the emergence of ciprofloxacin resistance in the outbreak

Tc-99m-tamoxifen: A novel diagnostic imaging agent for estrogen receptor-expressing breast cancer patients

PURPOSEThe aim of the study was to radiolabel, characterize, and perform in vitro and in vivo assessment of Technetium-99m (Tc-99m) tamoxifen for screening ER expressing lesions in breast cancer patients.METHODSIn this study, tamoxifen has been radiolabeled with Tc-99m via Tc-99m-tricarbonyl core. The characterization and quality control tests of Tc-99m-tamoxifen were performed. In vitro recep- tor binding and blocking studies were performed in both positive control (MCF-7) and negative control cell lines (MDA-MB-231). Normal biodistribution studies were performed in female Wistar albino rats. The pilot clinical studies were performed in 4 ER-expressing breast cancer patients. Of the 4 patients, 1 was on tamoxifen therapy. All 4 patients had also undergone Fluorine-18 fluorodeoxyglucose (F-18-FDG) positron emission tomography/computed tomography.RESULTSTamoxifen was radiolabeled with Tc-99m via Tc-99m-tricarbonyl core with more than 95% radio- chemical yield. Mass spectra showed a peak corresponding to the molecular weight of Tc-99m- tricarbonyl and Tc-99m-tamoxifen. The site of binding of Tc-99m-tricarbonyl with tamoxifen was determined by proton nuclear magnetic resonance. The Tc-99m-tamoxifen showed 30% binding with MCF-7 and only 1%-2% receptor binding with MDA-MB-231 cell lines. Also, the percentage of receptor binding was drastically reduced (up to 72%) when ER was saturated with 50 times the excess molar ratio of unlabeled tamoxifen. In a pilot patient study, Tc-99m-tamoxifen uptake was observed in primary and metastatic lesions. However, no uptake was observed in a patient who was on tamoxifen therapy. The uptake of F-18-FDG was noted in all the patients.CONCLUSIONTamoxifen was radiolabeled with an in-house-synthesized Tc-99m-tricarbonyl core. The radio- labeled complex has been characterized and evaluated for receptor specificity in in vitro and in vivo studies. Also, this is the first clinical study using Tc-99m-tamoxifen for imaging ER. More patients need to be evaluated to further explore the role of Tc-99m-tamoxifen in ER-expressing lesions

A study regarding awareness among mothers of children from 12 months to 23 months about growth charting and its determinants in rural area of Amritsar district

Abstract Despite of various nutritional health programmes, malnutrition among children remains the big health problem in India. Even after the universalization of ICDS, India has biggest burden of malnourished children in the world. Suboptimal utilization of services by mothers is a big challenge before all programmes. Utilization of services also depends upon the awareness regarding the service and its perceived usefulness among beneficiaries. Therefore, the present study was conducted to know the awareness about the growth chart and its determinants among rural mothers. Study was conducted on 186 mothers selected from three villages. Chi square test and F test were applied wherever necessary. Results showed low level of awareness (38.17%) among the mothers regarding growth charting. Majority of mothers reported peripheral health functionaries i.e. ASHA Introduction

A screen for conserved sequences with biased base composition identifies noncoding RNAs in the A–T rich genome of Plasmodium falciparum

Noncoding RNAs (ncRNAs) such as snRNAs, snoRNAs and microRNAs play important roles in transcription and translation control. These ncRNAs have yet to be discovered in the malarial parasite Plasmodium falciparum, an organism in which these basic biological processes are poorly understood. Inspired by a report by Klein et al., we initiated a bioinformatics screen to uncover several candidate ncRNAs from the parasite genome using two simple criteria: first, elevated GC content in the highly A–T rich intergenic regions of the P. falciparum genome and second, conservation of sequence homology between malaria parasite species. We show that all the annotated tRNAs can be successfully identified in our screen as well as several new candidates that show homology to snRNAs and snoRNAs, and ten candidate ncRNAs of unknown function. Three of the candidate snRNAs, a predicted selenocysteine tRNA and two candidates of unknown function are expressed in asexual stage parasites, further validating the screen. With these results, the biological processes underlying RNA-mediated regulation of transcription, translation and splicing can be studied in an important human pathogen.© Elsevie

Assessment of the effect of cigarette smoking on the different denture base material

Background: The present study was conducted to assess effect of cigarette smoking on different denture base material. Materials & Methods: The present study was conducted in the department of Prosthodontics. A total of sixty wax specimens in the shape of circular discs were prepared. These were divided into two groups. Group I (30) specimens were heat‑cured denture base materials and group II (30) specimens were flexible denture base materials. Both specimens were further divided into four subgroups of 15 each. Subgroup I was heat‑cured denture base material specimens (control group), subgroup II was flexible denture base material specimens (control group), subgroup III was heat‑cured denture base material specimens exposed to cigarette smoking (study group) and subgroup IV was flexible denture base material specimens exposed to cigarette smoking (study group). The initial (IRa) and final (FRa) surface roughness was measured before and after smoking test of the specimens. Results: It was observed that in group I, mean IR (µm) value was 0.182 and FR value was 0.572. In group II, mean IR (µm) value was 0.265 and FR value was 0.831. In group III, mean IR (µm) value was 0.195 and FR value was 1.892. In group IV, mean IR (µm) value was 0.291 and FR value was 1.892. The difference was significant (P< 0.05). Conclusion: The surface roughness of the specimens fabricated from the flexible denture base material was found to be more compared to heat‑cured denture base specimens after exposure to cigarette smoke. There is need to educate the patients regarding cleanliness of denture to avoid infection in the oral cavity

Evaluation of training manuals for health workers in India in context of kangaroo mother care

Background: Kangaroo mother care is an efficacious intervention in preventing mortality in low birth weight babies. With increasing focus on providing home based newborn care in India, it is pertinent to train the frontline healthcare workers in necessary skills for care of low birth weight babies. Objective: The current review was undertaken to evaluate the content of training manuals of frontline health workers in context of care of low birth weight (LBW) babies and Kangaroo Mother Care (KMC). Methods: A systematic extensive internet search was performed to identify training manuals available in public domain, and a targeted search was also done in the websites of Ministry of Health and Family Welfare, Government of India, National Institute of Health and Family Welfare, and National Health System Resource Centre. Manuals published in or after the year 2000 and those in the English language were included in the review. A quality assessment tool was devised and the manuals were finally classified as “poor”, “fair”, “good” quality. Results: The initial search yielded 107 potentially eligible documents, however, a total of eight training manuals were finally found to be eligible for content evaluation. The mean average score for all the eight manuals was 17.0 (out of a total score of 48) and thus they were “fair” quality (aggregated per cent score of 35.4). Out of the eight training manuals, six had separate section on care of the LBW babies, though content on breastfeeding and skin-to-skin contact was variable. None of the manuals provided case studies/ scenarios or introduced challenges to effective initiation and continuation of KMC. Conclusion: Current training manuals lack quality content on care of LBW babies and KMC and need to be upgraded with evidence-based information

Multiparametric Magnetic Resonance Imaging and Metabolic Characterization of Patient-Derived Xenograft Models of Clear Cell Renal Cell Carcinoma

Patient-derived xenografts (PDX) are high-fidelity cancer models typically credentialled by genomics, transcriptomics and proteomics. Characterization of metabolic reprogramming, a hallmark of cancer, is less frequent. Dysregulated metabolism is a key feature of clear cell renal cell carcinoma (ccRCC) and authentic preclinical models are needed to evaluate novel imaging and therapeutic approaches targeting metabolism. We characterized 5 PDX from high-grade or metastatic ccRCC by multiparametric magnetic resonance imaging (MRI) and steady state metabolic profiling and flux analysis. Similar to MRI of clinical ccRCC, T2-weighted images of orthotopic tumors of most PDX were homogeneous. The increased hyperintense (cystic) areas observed in one PDX mimicked the cystic phenotype typical of some RCC. The negligible hypointense (necrotic) areas of PDX grown under the highly vascularized renal capsule are beneficial for preclinical studies. Mean apparent diffusion coefficient (ADC) values were equivalent to those of ccRCC in human patients. Hyperpolarized (HP) [1-13C]pyruvate MRI of PDX showed high glycolytic activity typical of high-grade primary and metastatic ccRCC with considerable intra- and inter-tumoral variability, as has been observed in clinical HP MRI of ccRCC. Comparison of steady state metabolite concentrations and metabolic flux in [U-13C]glucose-labeled tumors highlighted the distinctive phenotypes of two PDX with elevated levels of numerous metabolites and increased fractional enrichment of lactate and/or glutamate, capturing the metabolic heterogeneity of glycolysis and the TCA cycle in clinical ccRCC. Culturing PDX cells and reimplanting to generate xenografts (XEN), or passaging PDX in vivo, altered some imaging and metabolic characteristics while transcription remained like that of the original PDX. These findings show that PDX are realistic models of ccRCC for imaging and metabolic studies but that the plasticity of metabolism must be considered when manipulating PDX for preclinical studies

Resistance to Androgen Deprivation Leads to Altered Metabolism in Human and Murine Prostate Cancer Cell and Tumor Models.

Currently, no clinical methods reliably predict the development of castration-resistant prostate cancer (CRPC) that occurs almost universally in men undergoing androgen deprivation therapy. Hyperpolarized (HP) 13C magnetic resonance imaging (MRI) could potentially detect the incipient emergence of CRPC based on early metabolic changes. To characterize metabolic shifts occurring upon the transition from androgen-dependent to castration-resistant prostate cancer (PCa), the metabolism of [U-13C]glucose and [U-13C]glutamine was analyzed by nuclear magnetic resonance spectroscopy. Comparison of steady-state metabolite concentrations and fractional enrichment in androgen-dependent LNCaP cells and transgenic adenocarcinoma of the murine prostate (TRAMP) murine tumors versus castration-resistant PC-3 cells and treatment-driven CRPC TRAMP tumors demonstrated that CRPC was associated with upregulation of glycolysis, tricarboxylic acid metabolism of pyruvate; and glutamine, glutaminolysis, and glutathione synthesis. These findings were supported by 13C isotopomer modeling showing increased flux through pyruvate dehydrogenase (PDH) and anaplerosis; enzymatic assays showing increased lactate dehydrogenase, PDH and glutaminase activity; and oxygen consumption measurements demonstrating increased dependence on anaplerotic fuel sources for mitochondrial respiration in CRPC. Consistent with ex vivo metabolomic studies, HP [1-13C]pyruvate distinguished androgen-dependent PCa from CRPC in cell and tumor models based on significantly increased HP [1-13C]lactate