Hemijski sastav nekih vrsta raža ulovljenih kočarenjem u mediteranskom zalivu Antalija (Turska)

The objective of this study is to determine the proximate analysis of some ray species caught by trawling in the Gulf of Antalya. Proximate analyses revealed non significant (P>0.05), differences in moisture among brown ray, thornback ray, common stingray and spiny butterfly ray. Of all the species analyses spiny butterfly ray had the highest protein content (22.46±2.18%). Common stingray and spiny butterfly ray had significantly different (P<0.05) levels of lipids compared to the others. Common stingray also had the highest ash content (2.96±0.02%) compared to the others. Therefore, it was concluded that discard fish species such as brown ray, thornback ray, common stingray and spiny butterfly ray would be use as a source of protein for human diet

False positivity for marijuana in immunoassay analysis due to Efavirenz use. A case report

WOS: 000378754600011Cross-reaction is an important problem with immunoassays that may lead to interferences and potentially yield wrong results. This case study describes a false positive tetrahydrocannabinol (THC) screening with CEDIA immunoassay reagents after the use of efavirenz for anti-retroviral therapy. A 31-year-old HIV (+) probationer transsexual was admitted to Ege University Institute of Drug Abuse, Toxicology and Pharmaceutical Sciences Toxicology Laboratory due to marijuana abuse. He had been using 600 mg/day Efavirenz to overcome his disease. As the routine application in the laboratory, the urine specimens of the probationer were first screened for drugs of abuse using CEDIA reagents. The analysis results were all found to be positive. Subsequently, the urine specimens were also analyzed for their THC content with another urine screening immunoassay kit using DRI (Diagnostic Reagent Inc.) reagents. The results were found to be negative. In order to ensure a correct result, the confirmation of the analysis was performed with the gas chromatography-mass spectrometer for the urine samples and the results were found to be below the detection limit (LOD < 1.97 ng/mL), Additionally a hair sample belonging to probationer was obtained, analyzed with a fully validated method and the result was also found to be negative. Therefore, it was concluded that the result obtained with CEDIA reagents was false positive for THC which is, and was caused by Efavirenz use by the probationer. The results demonstrated that there is a strong need for the careful interpretation of the analysis results by an experienced forensic toxicologist to decrease the chances of obtaining wrong results

Effect of cooking conditions on cholesterol oxidation and astaxanthin in dried salted shrimp

Dried salted shrimp is a product made from raw shrimps, which are usually cooked and dried under direct sunlight. Brine cooking is an important step during the production of dried salted shrimp as it promotes changes that affect the product\u2019s end quality. The aim of this study was to evaluate the effect of brine concentration and boiling time on cholesterol oxidation products (COPs) formation and the concomitant changes in astaxanthin content and fatty acid profile in shrimp during cooking, sun drying, and storage. Boiling conditions did not affect COPs formation in shrimp after cooking. However, increased brine concentration and boiling time promoted high astaxanthin retention in cooked shrimp. During the first 24 h of sun drying, COPs formation in dried salted shrimp was influenced by the interaction between brine concentration and boiling time; in fact, the lowest COPs levels were observed in samples boiled at low brine concentration and short cooking times, as well as those boiled in brine with high salt concentration and long cooking times. Most astaxanthin (ca. 78%) present in cooked shrimp was degraded during solar drying. During storage, PUFA decreased, and a concomitant astaxanthin degradation and COPs formation in dried salted shrimp were observed. Neither boiling time nor storage at dark of dried salted shrimp prevented these changes