2,368 research outputs found
Potential for ultrafast dynamic chemical imaging with few-cycle infrared lasers
We studied the photoelectron spectra generated by an intense few-cycle
infrared laser pulse. By focusing on the angular distributions of the back
rescattered high energy photoelectrons, we show that accurate differential
elastic scattering cross sections of the target ion by free electrons can be
extracted. Since the incident direction and the energy of the free electrons
can be easily changed by manipulating the laser's polarization, intensity, and
wavelength, these extracted elastic scattering cross sections, in combination
with more advanced inversion algorithms, may be used to reconstruct the
effective single-scattering potential of the molecule, thus opening up the
possibility of using few-cycle infrared lasers as powerful table-top tools for
imaging chemical and biological transformations, with the desired unprecedented
temporal and spatial resolutions.Comment: 16 pages, 6 figure
Estimation of carrier life time from intrinsic photoluminescence of ZnO
Comprehensive knowledge of the optical properties, particularly of the room
temperature (RT) photoluminescence (PL), of ZnO is essential for the future employment of this wideband
gap (~3.3 eV at 300 K) II-VI compound semiconductor in photonic and optoelectronic device
structures [1]. Hence, vigorous research activities on ZnO thin films, epilayers, and crystals took place
during the last two decades, encompassing a vast variety of effects and phenomena such as birefringence,
photocurrent, PL including sub-band gap emission, reflectance, transmittance, excitonic properties,
Raman modes, and absorption edge steepness [1-4]. However, despite that large body of knowledge and
its essential importance for light emitting processes, a discussion of the ZnO PL lineshape is not found
in the literature [5]
Platelet-activating Factor, a Molecular Sensor for Cellular Damage, Activates Systemic Immune Suppression
Ultraviolet (UV) radiation plays a critical role in the induction of nonmelanoma skin cancer. UV radiation is also immune suppressive, and the immune suppression induced by UV irradiation has been identified as a major risk factor for skin cancer induction. Previously, we showed that UV exposure activates a cytokine cascade involving prostaglandin (PG)E2, interleukin (IL)-4, and IL-10 that induces immune suppression. However, the earliest molecular events that occur immediately after UV exposure, especially those upstream of PGE2, are not well defined. UV-irradiated keratinocytes secrete the inflammatory phospholipid mediator, platelet-activating factor (PAF). Because PAF upregulates the production of immunomodulatory compounds, including PGE2, we tested the hypothesis that UV-induced PAF activates cytokine production and initiates UV-induced immune suppression. Both UV and PAF activated cyclooxygenase (COX)-2 and IL-10 reporter gene construct transcription. PAF mimicked the effects of UV in vivo and suppressed delayed-type hypersensitivity (DTH). Furthermore, immune suppression was blocked when UV-irradiated mice were injected with PAF receptor antagonists. In addition to the well-known role of PAF as a proinflammatory lipid mediator, we propose that the PAF receptor senses cellular damage through the recognition of PAF and/or PAF-like molecules, such as oxidized phosphatidylcholine, which activates cytokine transcription and induces systemic immune suppression
Intrinsic photoluminescence stokes shift in thin-film cadmium sulfide
Exciting a semiconductor through light absorption produces photoluminescence (PL).
In general, the emitted energy is lower than the energy absorbed. The phenomenon, first discovered
in the nineteenth century, is known as Stokes shift energy [1]. The change in energy (AS t o k e s), crucial
for the information about the phonon relaxation in the material and with importance in light emitting
devices, has not been investigated experimentally very systematically [2]. In this project, we present the
observation of the intrinsic photoluminescence Stokes shift in a semiconductor
Cluster ionization via two-plasmon excitation
We calculate the two-photon ionization of clusters for photon energies near
the surface plasmon resonance. The results are expressed in terms of the
ionization rate of a double plasmon excitation, which is calculated
perturbatively. For the conditions of the experiment by Schlipper et al., we
find an ionization rate of the order of 0.05-0.10 fs^(-1). This rate is used to
determine the ionization probability in an external field in terms of the
number of photons absorbed and the duration of the field. The probability also
depends on the damping rate of the surface plasmon. Agreement with experiment
can only be achieved if the plasmon damping is considerably smaller than its
observed width in the room-temperature single-photon absorption spectrum.Comment: 17 pages and 6 PostScript figure
P682Preserved contractile function of unloaded cardiomyocytes despite diminished sarcomere size is associated with troponin I activation
Objective: Myocardial unloading with ventricular assist devices in patients with severe heart failure (HF) can lead to reversal of certain aspects of pathological remodeling. However, these effects do not translate into recovery of myocardial function in the human heart, possibly due to detrimental atrophic processes also elicited through unloading. We have studied the effects of long-term unloading on sarcomeric morphology and function in a small animal model of ventricular unloading, heterotopic heart transplantation (HTX) in rats. Methods: Native rat hearts were unloaded via HTX for 30 days, CMs from control and unloaded hearts were isolated (n=8 hearts/>250 individual cells/group). CM overall size was determined, sarcomere length/contractility assessed and Calcium transients as well as E-C coupling gain analyzed in patch-clamped CMs. Additionally, phosphorylation of Troponin I, indicative of sarcomere activation, was measured with western blotting. Results: CM cross-sectional area was diminished in unloaded cells by about one third (2787±345 vs 1993±230 μm2) as was cell capacitance in patched cells. Accordingly, baseline sarcomere length was significantly reduced by ~0.2μm (Figure). However, this reduction did not diminish contractile function: fractional shortening was significantly higher in unloaded CMs (8.0 ± 3 % vs 6.6 ± 2.5 % in CTR, p = 0.01). Departure velocity of the transients was similar (-135.2 ± 48 vs -119.4 ± 40 dL/dt), and return velocity was slightly increased in unloaded cells (120.7 ± 54 vs 94.0 ± 46 dL/dt, p < 0.05), indicating preserved relaxation. Calcium transient amplitudes and current-voltage relationship under basal condition and isoproterenol stimulation was not changed. Troponin I phosphorylation was elevated and may contribute to the maintenance of sarcomeric function in long-term unloaded CMs. Conclusion: Although there are limitations regarding assessment of contractility in isolated cells, we may conclude that the considerable size reduction in CMs induced by unloading does not translate into diminished contractile function or E-C couplin
Platelet-activating factor is crucial in psoralen and ultraviolet A-induced immune suppression, inflammation, and apoptosis.
Psoralen plus UVA (PUVA) is used as a very effective treatment modality for various diseases, including psoriasis and cutaneous T-cell lymphoma. PUVA-induced immune suppression and/or apoptosis are thought to be responsible for the therapeutic action. However, the molecular mechanisms by which PUVA acts are not well understood. We have previously identified platelet-activating factor (PAF), a potent phospholipid mediator, as a crucial substance triggering ultraviolet B radiation-induced immune suppression. In this study, we used PAF receptor knockout mice, a selective PAF receptor antagonist, a COX-2 inhibitor (presumably blocking downstream effects of PAF), and PAF-like molecules to test the role of PAF receptor binding in PUVA treatment. We found that activation of the PAF pathway is crucial for PUVA-induced immune suppression (as measured by suppression of delayed type hypersensitivity to Candida albicans) and that it plays a role in skin inflammation and apoptosis. Downstream of PAF, interleukin-10 was involved in PUVA-induced immune suppression but not inflammation. Better understanding of PUVA\u27s mechanisms may offer the opportunity to dissect the therapeutic from the detrimental (ie, carcinogenic) effects and/or to develop new drugs (eg, using the PAF pathway) that act like PUVA but have fewer side effects
Tests of a Laser Ion Source at the Heidelberg Electron Beam Ion Trap
A laser ion source (LIS) has been designed and successfully tested for loading the Heidelberg electron beam ion trap (H-EBIT) with ions of practically all solid-state elements. A pulsed YAG:Nd3þ laser (30 mJ, 8 ns) is used to produce plasma from a solid target. Lowly charged ions are extracted from the plasma and accelerated by a short high-voltage pulse, generating a pulsed ion beam with energy of up to 6 keV per charge. The ion beam is transported into the EBIT, decelerated and captured within it by switching the trapping electrode potentials. The electron beam further ionizes the trapped ions to the desired highly charged states. The experimental setup is described together with the source parameters measured for different target materials. X-ray spectra of up to He-like highly charged ions (Cu, Mo, Pb, Al and Ge) produced in the EBIT after injection from the LIS have been measured. The reliability and convenience of this method allows the use of the LIS to produce highly charged ions of both insulating and conducting solid elements with an EBIT as needed for different experiments
Laser Induced Non-Sequential Double Ionization Investigated at and Below the Threshold for Electron Impact Ionization
We use correlated electron–ion momentum measurements to investigate laserinduced non-sequential double ionization of Ar and Ne. Light intensities are chosen in a regime at and below the threshold where, within the rescattering model, electron impact ionization of the singly charged ion core is expected to become energetically forbidden. Yet we find Ar2+ ion momentum distributions and an electron–electron momentum correlation indicative of direct impactionization. Within the quasistatic model this may be understood by assuming that the electric field of the light wave reduces the ionization potential of the singly charged ion core at the instant of scattering. The width of the projection of the ion momentum distribution onto an axis perpendicular to the light beam polarization vector is found to scalewiththe square root of the peak electric field strength in the light pulse. A scaling like this is not expected from the phase space available after electron impact ionization. It may indicate that the electric field at the instant of scattering is usually different fromzero and determines the transverse momentum distribution. A comparison of our experimental results with several theoretical results is give
Determination of the Carrier-Envelope Phase of Few-Cycle Laser Pulses with Terahertz-Emission Spectroscopy
The availability of few-cycle optical pulses opens a window to physical
phenomena occurring on the attosecond time scale. In order to take full
advantage of such pulses, it is crucial to measure and stabilise their
carrier-envelope (CE) phase, i.e., the phase difference between the carrier
wave and the envelope function. We introduce a novel approach to determine the
CE phase by down-conversion of the laser light to the terahertz (THz) frequency
range via plasma generation in ambient air, an isotropic medium where optical
rectification (down-conversion) in the forward direction is only possible if
the inversion symmetry is broken by electrical or optical means. We show that
few-cycle pulses directly produce a spatial charge asymmetry in the plasma. The
asymmetry, associated with THz emission, depends on the CE phase, which allows
for a determination of the phase by measurement of the amplitude and polarity
of the THz pulse
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