The endophytic fungus Piriformospora indica protects wheat from Fusarium crown rot disease in simulated UK autumn conditions

The root endophytic fungus Piriformospora indica (Sebacinacea) forms mutualistic symbioses with a broad range of host plants, increasing their biomass production and resistance to fungal pathogens. We evaluated the effect of P. indica on Fusarium crown rot disease of wheat, under in vitro and glasshouse conditions. Interaction of P. indica and Fusarium isolates under axenic culture conditions indicated no direct antagonistic activity of P. indica against Fusarium isolates. Seedlings of wheat were inoculated with P. indica and pathogenic Fusarium culmorum or F. graminearum and grown in sterilised soil-free medium or in a non-sterilised mix of soil and sand. Fusarium alone reduced emergence and led to visible browning and reduced root growth. Roots of seedlings in pots inoculated with both Fusarium isolates and P. indica were free of visible symptoms; seed emergence and root biomass were equivalent to the uninoculated. DNA was quantified by real-time polymerase chain reaction (qPCR). The ratio of Fusarium DNA to wheat DNA rose rapidly in the plants inoculated with Fusarium alone; isolates and species were not significantly different. P. indica inoculation reduced the ratio of Fusarium to host DNA in the root systems. The reduction increased with time. The ratio of P. indica to wheat DNA initially rose but then declined in root systems without Fusarium. With Fusarium, the ratio rose throughout the experiment. The absolute amount of Fusarium DNA in root systems increased in the absence of P. indica but was static in plants co-inoculated with P. indica

A novel Multi-permittivity Cylindrical Dielectric Resonator Antenna for Wideband Applications

In this paper, a novel multi-permittivity cylindrical dielectric resonator antenna for wideband application is presented. The multi-permittivity cylinder is formed by combining two different permittivity material sectors in such a way that each sector (with constant permittivity) is 90 degree apart. A direct microstrip line coupling terminated with T-stub at the open end is used to excite the multi-permittivity cylindrical dielectric resonator. The angular position of the multi sector dielectric resonator with respect to the longitudinal axis of the microstrip line and length of the additional strip at the open end of the feeding circuit is key parameters for wideband operation of the antenna. By optimizing all parameters of the proposed antenna, wideband impedance bandwidth of 56% (12.1 GHz - 21.65 GHz) is achieved. The average gain of the antenna throughout the bandwidth is 5.9 dB with good radiation properties in both E-plane and H-plane. A well matched simulation and experimental results show that the antenna is suitable for wideband applications

Two Hand Gesture Based 3D Navigation in Virtual Environments

Natural interaction is gaining popularity due to its simple, attractive, and realistic nature, which realizes direct Human Computer Interaction (HCI). In this paper, we presented a novel two hand gesture based interaction technique for 3 dimensional (3D) navigation in Virtual Environments (VEs). The system used computer vision techniques for the detection of hand gestures (colored thumbs) from real scene and performed different navigation (forward, backward, up, down, left, and right) tasks in the VE. The proposed technique also allow users to efficiently control speed during navigation. The proposed technique is implemented via a VE for experimental purposes. Forty (40) participants performed the experimental study. Experiments revealed that the proposed technique is feasible, easy to learn and use, having less cognitive load on users. Finally gesture recognition engines were used to assess the accuracy and performance of the proposed gestures. kNN achieved high accuracy rates (95.7%) as compared to SVM (95.3%). kNN also has high performance rates in terms of training time (3.16 secs) and prediction speed (6600 obs/sec) as compared to SVM with 6.40 secs and 2900 obs/sec

EFFECT OF PIGEON ORIGIN NEWCASTLE DISEASE VIRUS ON VARIOUS LIVER ENZYMES AND ASSOCIATED PATHOLOGICAL CHANGES IN EXPERIMENTALLY INFECTED PIGEONS

Newcastle disease virus (NDV) was isolated from a field outbreak in pigeons. The virus was characterized by haemagglutination test (HA) and confirmed by haemagglutination inhibition test (HAI). The pathotyping was done by mean death time (MDT), intracerebral pathogenicity index (ICPI) and intravenous pathogenicity index (IVPI). The ELD50 of the velogenic strain was 10-4.66/0.1 ml. Thirty-nine pigeons were randomly divided into three equal groups. Pigeons of one group were vaccinated with ND vaccine (LaSota strain) intraocularly after 14 days of procurement, while the other two groups served as vaccinated and non-vaccinated controls. Birds of these two groups were challenged with velogenic strain of field isolate of NDV 7 days post-vaccination. Birds were kept under observation for 15 days post-challenge. Haemorrhages and congestion were observed in trachea, lungs, liver, proventriculus and intestine of pigeons infected with NDV. Concentrations of AST, ALT and ALP did not differ among pigeons of the three groups

Phytochemical analysis of selected medicinal plants

Four medicinal plants including Ranunculus arvensis, Equisetum ravens, Carathamus lanatus and Fagonia critica were used for the study. All the plants were biologically active and were used for different types of ailments. Keeping in view their importance, this work was carried out to investigate the quantitative determination of their crude phytochemicals, vitamins and protein contents. The quantitative determination of crude phytochemicals (alkaloids, total phenols, flavonoids and saponins) vitamins (riboflavin, vitamin C, niacin and pectin) and protein were determined in the aforementioned herbs. The phytochemicals including alkaloids, total phenols, flavonoids and saponins were determined quantitatively using literature methods. Vitamins were measured using a UV/ visible spectrophotometer (UV- 1601 Shamidzu) and the protein was determined by the Micro KJeldahl’s method (Horwitz et al., 2000). The studied plants showed variable amounts of phytochemicals, vitamins and protein contents. The study is very important, in that it intended to show the contents of the studied medicinal herbs and also provide a scientific data base line which is of particular importance for the local practioners as well as for the local people using these herbs for a variety of body disorders.Key word: Phytochemical analysis, medicinal plants, Pakistan

Analysis of HMWGS of historical set of Pakistani bread wheat varieties using SDS-PAGE

In the present study an attempt has been made to characterize thirty bread-wheat varieties of Pakistan for High Molecular Weight Glutenin Subunits (HMW- GS). Glutenin proteins form a continuous proteinaceous matrix in the cells and form a continuous viscoelastic network during the mixing process of dough development. Glutenin consists of High Molecular Weight (HMW) and Low Molecular Weight (LMW) subunits. The HMW Glutenin Subunits (HMW-GS) are chiefly vital for determining doughelasticity. The core objective of our research work was to inspect the glutenin subunits by sodium dodecyl-sulfate polyacrylamide gel-electrophoresis (SDS-PAGE) and compare the banding pattern withChinese Spring High-Molecular-Weight Glutenin Subunits (HMW-GS). The bands were numbered according to Payne's numbering system and varieties were accordingly assigned theoretical quality scores. All the tested varieties indicated null allele for gluA1 locus, 17 + 18 for gluB1 locus and 2 + 12 for gluD1 locus. This result indicating that all varieties have similar bread making quality alleles at HMWGS loci. The varieties containing 5 + 10 HMWGS allele at gluD1 locus have better bread making quality. Better bread making wheat varieties may be produced by crossing the local varieties of gluA1 locus, 17 + 18 for gluB1 locus and 2 + 12 for gluD1 locus with 5 + 10 HMWGS allele at gluD1 locus

Percutaneous Intervention or Bypass Graft for Left Main Coronary Artery Disease? A Systematic Review and Meta-Analysis

Background. The safety and efficacy of percutaneous coronary intervention (PCI) versus coronary artery bypass grafting (CABG) for stable left main coronary artery disease (LMCAD) remains controversial.Methods. Digital databases were searched to compare the major adverse cardiovascular and cerebrovascular events (MACCE) and its components. A random effect model was used to compute an unadjusted odds ratio (OR).Results. A total of 43 studies (37 observational and 6 RCTs) consisting of 29,187 patients (PCI 13,709 and CABG 15,478) were identified. The 30-day rate of MACCE (OR, 0.56; 95% CI, 0.42-0.76;p = 0.0002) and all-cause mortality (OR, 0.52; 95% CI, 0.30-0.91;p = 0.02) was significantly lower in the PCI group. There was no significant difference in the rate of myocardial infarction (MI) (p = 0.17) and revascularization (p = 0.12). At 5 years, CABG was favored due to a significantly lower rate of MACCE (OR, 1.67; 95% CI, 1.18-2.36;p = <0.04), MI (OR, 1.67; 95% CI, 1.35-2.06;p = <0.00001), and revascularization (OR, 2.80; 95% CI, 2.18-3.60;p = <0.00001), respectively. PCI was associated with a lower overall rate of a stroke, while the risk of all-cause mortality was not significantly different between the two groups at 1- (p = 0.75), 5- (p = 0.72), and 10-years (p = 0.20). The Kaplan-Meier curve reconstruction revealed substantial variations over time; the 5-year incidence of MACCE was 38% with CABG, significantly lower than 45% with PCI (p = <0.00001).Conclusion. PCI might offer early safety advantages, while CABG provides greater durability in terms of lower long-term risk of ischemic events. There appears to be an equivalent risk for all-cause mortality

Effect of “Garbha Cintamani Rasa”, an ayurvedic formulation on lipid profile, liver function and kidney function parameters of rat plasma after chronic administration

Garbha Cintamani Rasa (GCM), a classical Ayurvedic preparation which is used in puerperal complications, was studied for its effect on liver function, kidney function and lipid profile after chronic administrations into the biological system. The experimental animal model was rat of both sexes. Triglycerides (TG) content was observed to be increased very high significantly in male as well as female group. Statistically, irrespective of sexes, high significant increase was found in very low density lipoprotein (VLDL). Decrease of total cholesterol (TC), low density lipoprotein (LDL) and high density lipoprotein (HDL) were negligible in male rats. Similar results were shown by female rats also. In both of the sexes, total protein and albumin content of plasma were increased very high significantly. In case of bilirubin, the increase was negligible for all sexes. The serum Effect of “Garbha Cintamani Rasa”, an Ayurvedic Formulation on Lipid Profile, Liver Function and Kidney Function Parameters of Rat Plasma after Chronic Administration. 26 glutamic pyruvic transaminase (sGPT), serum glutamic oxaloacetic transaminase (sGOT) and alkaline phosphatase (ALP) content in the plasma were decreased very high significantly in both of the experimental groups. Creatinine, urea and uric acid were decreased in male and female where only change of creatinine level was significant for both of the sexes. The changes of plasma parameters of both sexes were in similar fashion when one sex group is compared to opposite sex group.I. Jahan Bulbul, M. Obayed Ullah, M. Azizur Rahaman, K. Ashfaqur Rahman, A. Kumar Paul, M.S.K. Choudhur

Effects of Seasonal Precipitation on the Amount of Seepage-A Case Study of Tunnel 3 of Bazai Irrigation Project Khyber Pakhtunkhwa

Infiltration of water into tunnel within a harsh geological formation is a vital issue in tunnelling. The consequence impacts due to seepage include tunnel rock instability, pore-water pressure imposition and diminution of operational capacity. The spatial variation in rainfall due to climate change intensifies the threat to tunnel stability. Likewise, to understand the impacts of climate change scenarios on the seepage of tunnel 3 of the Bazai irrigation project was numerically simulated in SEEP/W software by manipulating the rainfall data. The net annual precipitation is followed by two sets of rainfall data i.e., dry and wet season precipitation depending upon the magnitude of rainfall. The analysis revealed that most of the seepage occurred in the unlined portion. In order to determine the future impacts of precipitation on seepage quantity, the wet season precipitation was further increased by 10% and 50% for A1B and B2 conditions respectively. The seepage quantity into the tunnel increases with variation in precipitation patterns. To reduce the risk to tunnel stability, the model was also treated with cement-bentonite grout and bentonite slurry containing 6% solids. The performance of both grouting techniques leads to noticeable seepage deduction. The study further suggests that cement-bentonite is more effective in seepage remediation