Data for: Effects of a pathogenic ETEC strain and a probiotic Enterococcus faecium strain on the inflammasome response in porcine dendritic cells

S1 Table. Results of RT-qPCR for NLRP6. Cells were primed with LPS (A) or left unprimed (B) and incubated with either E. faecium (Ecf) or ETEC alone, or Ecf + ETEC. N = 3 - 5 independent experiments.S2 Table. Results of RT-qPCR for NLRC4. Cells were primed with LPS (A) or left unprimed (B) and incubated with either E. faecium (Ecf) or ETEC alone, or Ecf + ETEC. N = 3 - 5 independent experiments.S3 Table. Protein detection of IL-18. IL-18 expression [pg/mL] detected by ELISA (minimum detectable dose 80 pg/mL) in supernatants of porcine MoDC primed with LPS (A) or left unprimed (B) and incubated with either E. faecium (Ecf) or ETEC alone, or Ecf + ETEC. N = 4 - 5 independent experiments

Data for: Effects of a pathogenic ETEC strain and a probiotic Enterococcus faecium strain on the inflammasome response in porcine dendritic cells

S1 Table. Results of RT-qPCR for NLRP6. Cells were primed with LPS (A) or left unprimed (B) and incubated with either E. faecium (Ecf) or ETEC alone, or Ecf + ETEC. N = 3 - 5 independent experiments.S2 Table. Results of RT-qPCR for NLRC4. Cells were primed with LPS (A) or left unprimed (B) and incubated with either E. faecium (Ecf) or ETEC alone, or Ecf + ETEC. N = 3 - 5 independent experiments.S3 Table. Protein detection of IL-18. IL-18 expression [pg/mL] detected by ELISA (minimum detectable dose 80 pg/mL) in supernatants of porcine MoDC primed with LPS (A) or left unprimed (B) and incubated with either E. faecium (Ecf) or ETEC alone, or Ecf + ETEC. N = 4 - 5 independent experiments.THIS DATASET IS ARCHIVED AT DANS/EASY, BUT NOT ACCESSIBLE HERE. TO VIEW A LIST OF FILES AND ACCESS THE FILES IN THIS DATASET CLICK ON THE DOI-LINK ABOV

Probiotics and antibiotics as additives for sows and piglets during nursery phase Probióticos e antibióticos como aditivos para matrizes e leitões na fase de creche

The purpose of this study was to evaluate the effects of the use of probiotics in diets for sows and piglets on performance and morphophysiologic parameters and the incidence of diarrhea in piglets during 28 days after weaning. A total of 120 pigs weaned at 21 days of age from 24 litters whose sows (primiparous) received probiotics or antibiotics from the 94th day of gestation until weaning. It was used a complete randomized design in a 2 × 3 factorial (probiotics or antibiotics for sows and three diets for piglets - antibiotics, probiotics or probiotic-antibiotic combination) with four replicates of five animals to evaluate the performance and incidence of diarrhea. At 28 days of experiment, two animals from each plot were sacrificed for histological parameter measurement of the pH of the stomach, jejunum and cecum. The association antibiotics+ probiotics in the diet of pigs resulted in greater weight gain and feed intake in animals born from of sows which received probiotics during the gestation and lactation phases. When females received antibiotics, the piglets receiving only probiotics showed higher consumption, but there were no differences for feed conversion. The use of antibiotics + probiotics in diets for piglets reduced the pH of the cecum and, when associated with the use of probiotics for sows, villous:crypt ratio in the jejunum increased. The use of growth promoters did not affect stomach pH neither the incidence of diarrhea. Supply of probiotics in the diet of sows during the gestation and lactation phases associated to the use in the diet of piglets after weaning is effective in maintaining animal performance, to histophysiological conditions in the gastrointestinal tract and control of diarrhea during the nursery phase.<br>O objetivo neste trabalho foi avaliar os efeitos do uso de probióticos em dietas para matrizes e leitões, sobre o desempenho, os parâmetros morfofisiológicos e a indicência de diarréia nos leitões durante 28 dias após o desmame. Foram utilizados 120 leitões desmamados aos 21 dias, provenientes de 24 leitegadas cujas matrizes (primíparas) receberam probióticos ou antibióticos a partir do 94º dia de gestação até o desmame. Foi utilizado um delineamento inteiramente casualizado em esquema fatorial 2 × 3 (probióticos ou antibióticos para matrizes e três dietas para leitões - antibióticos, probióticos ou associação antibiótico-probiótico) com quatro repetições de cinco animais para avaliação do desempenho e da incidência de diarreia. Aos 28 dias de experimento, dois animais de cada parcela foram sacrificados para mensuração de parâmetros histológicos do jejuno e pH do estômago e ceco. A associação probióticos + antibióticos na dieta dos leitões resultou em maior ganho de peso e consumo de ração nos animais filhos das matrizes que receberam probióticos durante as fases de gestação e lactação. Quando as fêmeas receberam antibióticos, os leitões que receberam apenas probióticos apresentaram maior consumo, porém não foram observadas diferenças na conversão alimentar. O uso de antibióticos + probióticos na dieta de leitões reduziu o pH do ceco e, quando associado ao uso de probióticos para matrizes, aumentou a relação vilosidade:cripta do jejuno. O uso de promotores de crescimento não influenciou o pH estomacal nem a incidência de diarreia. O fornecimento de probióticos na dieta das matrizes durante as fases de gestação e lactação em assossiação ao uso na dieta dos leitões após o desmame é eficaz em manter o desempenho dos animais, às condições histofisiológicas do trato gastrintestinal e o controle de diarreia durante a fase de creche

Effects of dietary fibre and protein on urea transport across the cecal mucosa of piglets

Item does not contain fulltextIn ruminants, gastrointestinal recycling of urea is acutely enhanced by fibre-rich diets that lead to high ruminal concentration of short chain fatty acids (SCFA), while high ammonia has inhibitory effects. This study attempted to clarify if urea flux to the porcine cecum is similarly regulated. Thirty-two weaned piglets were fed diets containing protein (P) of poor prececal digestibility and fibre (F) at high (H) or low levels (L) in a 2 x 2 factorial design. After slaughter, cecal content was analyzed and the cecal mucosa incubated in Ussing chambers to measure the effect of pH, SCFA and NH4 (+) on the flux rates of urea, short-circuit current (I sc) and tissue conductance (G t). NH4 (+) significantly enhanced I sc (from 0.5 +/- 0.2 to 1.2 +/- 0.1 muEq cm(-2) h(-1)). No acute effects of SCFA or ammonia on urea flux were observed. Tissue conductance was significantly lower in the high dietary fibre groups irrespective of the protein content. Only the HP-LF group emerged as different from all others in terms of urea flux (74 +/- 6 versus 53 +/- 3 nmol cm(-2) h(-1)), associated with higher cecal ammonia concentration and reduced fecal consistency. The data suggest that as in the rumen, uptake of ammonia by the cecum may involve electrogenic transport of the ionic form (NH4 (+)). In contrast to findings in the rumen, neither a high fibre diet nor acute addition of SCFA enhanced urea transport across the pig cecum. Instead, a HP-LF diet had stimulatory effects. A potential role for urea recycling in stabilizing luminal pH is discussed