187 research outputs found
Aktivnost arginaze u ovarijskim strukturama krava ĆĄvicarske smeÄe pasmine i njezinih kriĆŸanaca.
Arginase is the last enzyme of the urea cycle. It catalyses the hydrolysis of L-arginine to L-ornitine and urea. The aim of this study was to investigate the presence of arginase activity in ovarian structures such as: Graaf follicles, GF (Medium and Large size, M- and L-size), Corpus Haemorrhagicum (CH), and various types of Corpus Luteum (CL) such as: cyclic CL (CCL), 2-4 month pregnancy CL (2-4 MCL) and 4-7 month pregnancy CL (4-7 MCL). Ovarian tissues of 62 cows (7-10 years old and Brown Swiss or its cross-breeds), collected from a local slaughterhouse, were used as material. The materials were divided into 6 experimental groups, as follows: MGF group (n = 7), LGF group (n = 21), CH group (n = 7), CCL group (n = 6), 2-4 MCL group (n = 9) and 4-7 MCL group (n = 12). Arginase activities were measured as 0.056 ± 0.017, 0.100 ± 0.016, 2.517 ± 0.521, 0.827 ± 0.190, 0.674 ± 0.106 and 0.833 ± 0.093 U/mg protein in all groups, respectively. Arginase activity in the CH group was significantly higher than that in the CCL, 2-4 MCL and 4-7 MCL groups (P<0.001). The lowest enzyme activity was in the MGF and LGF groups. Hence, it was concluded that the arginase enzyme might play a crucial role in cell division, proliferation and differentiation in the ovarian tissues (especially the CH) of mature cows.Arginaza je posljednji enzim u ciklusu ureje koji katalizira hidrolizu L-arginina u L-ornitin i ureju. Cilj istraĆŸivanja bio je utvrditi prisutnost aktivnosti arginaze u ovarijskim strukturama kao ĆĄto su Graafovi folikuli, GF (srednje veliki - M i veliki - L), corpus haemorrhagicum (CH) i razliÄiti tipovi corpus luteum (CL) kao ĆĄto su cikliÄni (CCL), 2-4 mjeseca graviditetni (2-4 MCL) i 4-7 mjeseci graviditetni (4-7 MCL). Tkiva ovarija od 62 krave (ĆĄvicarske smeÄe pasmine i kriĆŸanaca u dobi od 7 do 10 godina) prikupljena su u lokalnoj klaonici. Materijali su bili podijeljeni u 6 pokusnih skupina kako slijedi: MGF skupina (n = 7), LGF skupina (n = 21), CH skupina (n = 7), CCL skupina (n = 6), 2-4 MCL skupina (n = 9) i 4-7 MCL skupina (n = 12). Slijedom navedenih skupina, aktivnost arginaze bila je 0,056 ± 0,017, 0,100 ± 0,016, 2,517 ± 0,521, 0,827 ± 0,190, 0,674 ± 0,106 i 0,833 ± 0,093 U/mg. Aktivnost arginaze u CH skupini bila je signifikantno viĆĄa u odnosu na skupine CCL, 2-4 MCL i 4-7 MCL (P<0,001). NajniĆŸa aktivnost enzima bila je u skupinama MGF i LGF. ZakljuÄeno je o moguÄoj kljuÄnoj ulozi aktivnosti enzima arginaze u diobi stanica, proliferaciji i diferencijaciji ovarijskih tkiva (osobito CH) kod odraslih krava
Clinical spectrum of early onset âMediterraneanâ (homozygous p.P131L mutation) mitochondrial neurogastrointestinal encephalomyopathy
Mitochondrial neurogastrointestinal encephalomyopathy (MNGIE) is an autosomal recessive mitochondrial disorder characterized by cumulative and progressive gastrointestinal and neurological findings. This retrospective observational study, aimed to explore the time of presentation, diagnosis and clinical follow-up of 13 patients with a confirmed MNGIE disease of Mediterranean origin. The mean age of symptom onset was 7âyears (6 monthsâ21âyears) and the average diagnosis age was 15.4âyears ±8.4. Four of 13 patients (30%) died before 30âyears at the mean age of 19.7âyears ±6.8. Cachexia and gastrointestinal symptoms were observed in all patients (100%). The mean body mass index standard deviation score at diagnosis was 4.8â±â2.8. At least three subocclusive episodes were presented in patients who died in last year of their life. The main neurological symptom found in most patients was peripheral neuropathy (92%). Ten patients (77%) had leukoencephalopathy and the remaining three patients without were under 10âyears of age. The new homozygous âMediterraneanâ TYMP mutation, p.P131L (c.392 Câ>âT) was associated with an early presentation and poor prognosis in nine patients (69%) from five separates families. Based on the observations from this Mediterranean MNGIE cohort, we propose that the unexplained abdominal pain combined with cachexia is an indicator of MNGIE. High-platelet counts and nerve conduction studies may be supportive laboratory findings and the frequent subocclusive episodes could be a negative prognostic factor for mortality. Finally, the homozygous p.P131L (c.392 Câ>âT) mutation could be associated with rapid progressive disease with poor prognosis
Elaboration and characterisation of novel low-cost adsorbents from grass-derived sulphonated lignin
AbstractThis study investigated the use of water-soluble sulphonated lignin (SL) extracted from grass, which has not been used before as a precursor of activated carbon (AC). Chemical activation of SL with three dehydrating salts (ZnCl2, KCl, Fe2(SO4)3·xH2O) at various salt concentrations (10%, 20%, 30%w/w), charring temperatures (600,700°C) and charring times (1,2h) has been carried out. The surface characteristics and removal efficiencies of cadmium, copper and zinc ions from aqueous solutions were affected by the activation conditions. The sulphonated lignin-based activated carbons (SLACs) with the highest specific surface area, total pore and micropore volume were produced at the lowest dehydrating salt concentration (10%w/w) and at 700°C and 2-h charring. These optimal sulphonated lignin-based ACs were named SLAC-ZC (optimal grass-derived SLAC activated by zinc chloride); SLAC-PC (optimal grass-derived SLAC activated by potassium chloride) and SLAC-FS (optimal grass-derived SLAC activated by ferric sulphate). The central composite design and surface response methodology of different SLACs characteristics showed that the optimal responses were achieved at the same operating conditions. These SLACs also achieved the highest removal efficiencies of Cd2+, Cu2+ and Zn2+ ions from aqueous solutions. The chemical activation had significantly increased the total porosity, microporosity and surface area of water-soluble SL. The activation mechanism depended on the used dehydrating salt where the porosity developed by the dehydration effect of ZnCl2, and by a series of hydrolysis and redox reactions for the other two salts. The results of this research demonstrated that water-soluble SL has a great potential as a novel precursor for the production of activated carbons
Open Problems in (Hyper)Graph Decomposition
Large networks are useful in a wide range of applications. Sometimes problem
instances are composed of billions of entities. Decomposing and analyzing these
structures helps us gain new insights about our surroundings. Even if the final
application concerns a different problem (such as traversal, finding paths,
trees, and flows), decomposing large graphs is often an important subproblem
for complexity reduction or parallelization. This report is a summary of
discussions that happened at Dagstuhl seminar 23331 on "Recent Trends in Graph
Decomposition" and presents currently open problems and future directions in
the area of (hyper)graph decomposition
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Hematopoietic stem cell transplantation with reduced toxicity conditioning regimen in mitochondrial neurogastrointestinal encephalopathy syndrome
Background
Mitochondrial neurogastrointestinal encephalomyopathy (MNGIE) is a rare autosomal recessive disorder due to mutations in the TYMP gene. Clinical findings are characterized by neurologic manifestations and severe gastrointestinal dysfunction. The syndrome is usually fatal, the most effective treatment appears to be hematopoietic stem cell transplantation (HSCT).
Procedure
In this retrospective study, we evaluated HSCT that was performed using a reduced toxicity myeloablative conditioning regimen in patients with MNGIE at our center.
Results
A total of six allogeneic transplant procedures were performed in four patients. Three patients had fully matched donors, and one patient had a haploidentical donor. Treosulfan-based myeloablative conditioning regimen was applied in five of six transplants. Bone marrow was used as a stem cell source. One patient is being followed up in the 4th year of posttransplant with full chimeric and without graft versus host disease (GVHD). One patient died of acute stage IV gastrointestinal system GVHD. Two patients underwent second transplantation due to engraftment failure, one of which was the patient who had a haploidentical transplant.
Conclusions
Treosulfan-based regimen is well tolerated, although engraftment failure with this conditioning regimen can be a significant problem. We share our haploidentical transplant experience, which will be the first reported case in the literature
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