51 research outputs found
Photon pair-state preparation with tailored spectral properties by spontaneous four-wave mixing in photonic-crystal fiber
We study theoretically the generation of photon pairs by spontaneous
four-wave mixing (SFWM) in photonic crystal optical fiber. We show that it is
possible to engineer two-photon states with specific spectral correlation
(``entanglement'') properties suitable for quantum information processing
applications. We focus on the case exhibiting no spectral correlations in the
two-photon component of the state, which we call factorability, and which
allows heralding of single-photon pure-state wave packets without the need for
spectral post filtering. We show that spontaneous four wave mixing exhibits a
remarkable flexibility, permitting a wider class of two-photon states,
including ultra-broadband, highly-anticorrelated states.Comment: 17 pages, 7 figures, submitte
From quantum pulse gate to quantum pulse shaper -- enigneered frequency conversion in nonlinear optical waveguides
Full control over the spatio-temporal structure of quantum states of light is
an important goal in quantum optics, to generate for instance single-mode
quantum pulses or to encode information on multiple modes, enhancing channel
capacities. Quantum light pulses feature an inherent, rich spectral
broadband-mode structure. In recent years, exploring the use of integrated
optics as well as source-engineering has led to a deep understanding of the
pulse-mode structure of guided quantum states of light. In addition, several
groups have started to investigate the manipulation of quantum states by means
of single-photon frequency conversion. In this paper we explore new routes
towards complete control of the inherent pulse-modes of ultrafast pulsed
quantum states by employing specifically designed nonlinear waveguides with
adapted dispersion properties. Starting from our recently proposed quantum
pulse gate (QPG) we further generalize the concept of spatio-spectral
engineering for arbitrary \chitwo-based quantum processes. We analyse the
sum-frequency generation based QPG and introduce the difference-frequency
generation based quantum pulse shaper (QPS). Together, these versatile and
robust integrated optics devices allow for arbitrary manipulations of the
pulse-mode structure of ultrafast pulsed quantum states. The QPG can be
utilized to select an arbitrary pulse mode from a multimode input state,
whereas the QPS enables the generation of specific pulse modes from an input
wavepacket with Gaussian-shaped spectrum.Comment: 21 pages, 9 figure
Spectral structure and decompositions of optical states, and their applications
We discuss the spectral structure and decomposition of multi-photon states.
Ordinarily `multi-photon states' and `Fock states' are regarded as synonymous.
However, when the spectral degrees of freedom are included this is not the
case, and the class of `multi-photon' states is much broader than the class of
`Fock' states. We discuss the criteria for a state to be considered a Fock
state. We then address the decomposition of general multi-photon states into
bases of orthogonal eigenmodes, building on existing multi-mode theory, and
introduce an occupation number representation that provides an elegant
description of such states that in many situations simplifies calculations.
Finally we apply this technique to several example situations, which are highly
relevant for state of the art experiments. These include Hong-Ou-Mandel
interference, spectral filtering, finite bandwidth photo-detection, homodyne
detection and the conditional preparation of Schr\"odinger Kitten and Fock
states. Our techniques allow for very simple descriptions of each of these
examples.Comment: 12 page
Avalanche Photo-Detection for High Data Rate Applications
Avalanche photo detection is commonly used in applications which require
single photon sensitivity. We examine the limits of using avalanche photo
diodes (APD) for characterising photon statistics at high data rates. To
identify the regime of linear APD operation we employ a ps-pulsed diode laser
with variable repetition rates between 0.5MHz and 80MHz. We modify the mean
optical power of the coherent pulses by applying different levels of
well-calibrated attenuation. The linearity at high repetition rates is limited
by the APD dead time and a non-linear response arises at higher photon-numbers
due to multiphoton events. Assuming Poissonian input light statistics we
ascertain the effective mean photon-number of the incident light with high
accuracy. Time multiplexed detectors (TMD) allow to accomplish photon- number
resolution by photon chopping. This detection setup extends the linear response
function to higher photon-numbers and statistical methods may be used to
compensate for non-linearity. We investigated this effect, compare it to the
single APD case and show the validity of the convolution treatment in the TMD
data analysis.Comment: 16 pages, 5 figure
Integrated Photonic Sensing
Loss is a critical roadblock to achieving photonic quantum-enhanced
technologies. We explore a modular platform for implementing integrated
photonics experiments and consider the effects of loss at different stages of
these experiments, including state preparation, manipulation and measurement.
We frame our discussion mainly in the context of quantum sensing and focus
particularly on the use of loss-tolerant Holland-Burnett states for optical
phase estimation. In particular, we discuss spontaneous four-wave mixing in
standard birefringent fibre as a source of pure, heralded single photons and
present methods of optimising such sources. We also outline a route to
programmable circuits which allow the control of photonic interactions even in
the presence of fabrication imperfections and describe a ratiometric
characterisation method for beam splitters which allows the characterisation of
complex circuits without the need for full process tomography. Finally, we
present a framework for performing state tomography on heralded states using
lossy measurement devices. This is motivated by a calculation of the effects of
fabrication imperfections on precision measurement using Holland-Burnett
states.Comment: 19 pages, 7 figure
Within-Host Evolution of Burkholderia pseudomallei in Four Cases of Acute Melioidosis
Little is currently known about bacterial pathogen evolution and adaptation within the host during acute infection. Previous studies of Burkholderia pseudomallei, the etiologic agent of melioidosis, have shown that this opportunistic pathogen mutates rapidly both in vitro and in vivo at tandemly repeated loci, making this organism a relevant model for studying short-term evolution. In the current study, B. pseudomallei isolates cultured from multiple body sites from four Thai patients with disseminated melioidosis were subjected to fine-scale genotyping using multilocus variable-number tandem repeat analysis (MLVA). In order to understand and model the in vivo variable-number tandem repeat (VNTR) mutational process, we characterized the patterns and rates of mutations in vitro through parallel serial passage experiments of B. pseudomallei. Despite the short period of infection, substantial divergence from the putative founder genotype was observed in all four melioidosis cases. This study presents a paradigm for examining bacterial evolution over the short timescale of an acute infection. Further studies are required to determine whether the mutational process leads to phenotypic alterations that impact upon bacterial fitness in vivo. Our findings have important implications for future sampling strategies, since colonies in a single clinical sample may be genetically heterogeneous, and organisms in a culture taken late in the infective process may have undergone considerable genetic change compared with the founder inoculum
BurkDiff: A Real-Time PCR Allelic Discrimination Assay for Burkholderia Pseudomallei and B. mallei
A real-time PCR assay, BurkDiff, was designed to target a unique conserved region in the B. pseudomallei and B. mallei genomes containing a SNP that differentiates the two species. Sensitivity and specificity were assessed by screening BurkDiff across 469 isolates of B. pseudomallei, 49 isolates of B. mallei, and 390 isolates of clinically relevant non-target species. Concordance of results with traditional speciation methods and no cross-reactivity to non-target species show BurkDiff is a robust, highly validated assay for the detection and differentiation of B. pseudomallei and B. mallei
Experiential learning and the acquisition of managerial tacit knowledge
Tacit knowledge is believed to be one factor that distinguishes successful managers from others. We sought to determine whether levels of accumulated managerial tacit knowledge (LAMTK) were associated with managers' dominant learning styles. Instruments used in the study, involving 356 Malaysian public sector employees, included Sternberg et al.'s (2000) Tacit Knowledge Inventory for Managers and a normative version of Kolb's (1999a) Learning Styles Inventory (LSI-Ill). Findings suggest that LAMTK is independent of the length of subjects' general work experience, but positively related to the amount of time spent working in a management context. Learning styles also had a significant relationship. Subjects who spent most of their time performing management functions and whose dominant learning styles were accommodating had significantly higher LAMTK than those with different learning styles. We also found support for the belief that learners with a strong preference for all four different abilities defined in Kolb's learning theory may be critical for effective experiential learning
Characterization of Ceftazidime Resistance Mechanisms in Clinical Isolates of Burkholderia pseudomallei from Australia
Burkholderia pseudomallei is a Gram-negative bacterium that causes the serious human disease, melioidosis. There is no vaccine against melioidosis and it can be fatal if not treated with a specific antibiotic regimen, which typically includes the third-generation cephalosporin, ceftazidime (CAZ). There have been several resistance mechanisms described for B. pseudomallei, of which the best described are amino acid changes that alter substrate specificity in the highly conserved class A Ξ²-lactamase, PenA. In the current study, we sequenced penA from isolates sequentially derived from two melioidosis patients with wild-type (1.5 Β΅g/mL) and, subsequently, resistant (16 or β₯256 Β΅g/mL) CAZ phenotypes. We identified two single-nucleotide polymorphisms (SNPs) that directly increased CAZ hydrolysis. One SNP caused an amino acid substitution (C69Y) near the active site of PenA, whereas a second novel SNP was found within the penA promoter region. In both instances, the CAZ resistance phenotype corresponded directly with the SNP genotype. Interestingly, these SNPs appeared after infection and under selection from CAZ chemotherapy. Through heterologous cloning and expression, and subsequent allelic exchange in the native bacterium, we confirmed the role of penA in generating both low-level and high-level CAZ resistance in these clinical isolates. Similar to previous studies, the amino acid substitution altered substrate specificity to other Ξ²-lactams, suggesting a potential fitness cost associated with this mutation, a finding that could be exploited to improve therapeutic outcomes in patients harboring CAZ resistant B. pseudomallei. Our study is the first to functionally characterize CAZ resistance in clinical isolates of B. pseudomallei and to provide proven and clinically relevant signatures for monitoring the development of antibiotic resistance in this important pathogen
Gene Expression Profiles of Sporadic Canine Hemangiosarcoma Are Uniquely Associated with Breed
The role an individual's genetic background plays on phenotype and biological behavior of sporadic tumors remains incompletely understood. We showed previously that lymphomas from Golden Retrievers harbor defined, recurrent chromosomal aberrations that occur less frequently in lymphomas from other dog breeds, suggesting spontaneous canine tumors provide suitable models to define how heritable traits influence cancer genotypes. Here, we report a complementary approach using gene expression profiling in a naturally occurring endothelial sarcoma of dogs (hemangiosarcoma). Naturally occurring hemangiosarcomas of Golden Retrievers clustered separately from those of non-Golden Retrievers, with contributions from transcription factors, survival factors, and from pro-inflammatory and angiogenic genes, and which were exclusively present in hemangiosarcoma and not in other tumors or normal cells (i.e., they were not due simply to variation in these genes among breeds). Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) was among genes preferentially enriched within known pathways derived from gene set enrichment analysis when characterizing tumors from Golden Retrievers versus other breeds. Heightened VEGFR1 expression in these tumors also was apparent at the protein level and targeted inhibition of VEGFR1 increased proliferation of hemangiosarcoma cells derived from tumors of Golden Retrievers, but not from other breeds. Our results suggest heritable factors mold gene expression phenotypes, and consequently biological behavior in sporadic, naturally occurring tumors
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