Photogrammetric restitution of a presumed ancient Asclepius temple in Titani, Peloponnesos, Greece

Close range photogrammetry is a useful tool for the documentation and registration of archaeological sites. In this case, photogrammetric restitution is applied to a presumed Esclepion Classical temple site in Titani, Peloponnesos, Greece. The archaeological remains that are recorded and processed in this stage are small fragments of walls, made out of irregular shaped stones. The fragmentary remains and the need to record both the facades of the stones as well as the upper surfaces, complicate the photogrammetric recording and processing workflow. The use of 3D documentation is important for the documentation, conservation and possible further excavation of the site. Stereographic pictures in combination with terrestrial topographic measurements are processed in the photogrammetric software VirtuoZoTM. The stereo photographs were taken by a non-metric high resolution digital single lens reflex camera with a minimum overlap of 65 percent. Targets placed on the remains of the walls were measured by total station to obtain ground control points for the orientation of each 3D stereo model in an absolute coordinate system (HGRS87). The photogrammetric processing of the stereo models results in very accurate digital elevation models and orthophotos of the walls. Further combining of these final products and merging these products in a CAD software leads to a 3D presentation of the archaeological excavation, which can be further used to evolve this archaeological site

La céramique des tombes 121 et 135

Tytgat Christiane. La céramique des tombes 121 et 135. In: Bulletin de correspondance hellénique. Volume 103, livraison 2, 1979. pp. 742-749

Deux tombes géométriques de la Nécropole Nord d'Amathonte

Aupert Pierre, Tytgat Christiane. Deux tombes géométriques de la Nécropole Nord d'Amathonte. In: Bulletin de correspondance hellénique. Volume 108, livraison 2, 1984. pp. 619-653

Report on the activity of the Belgian School at Athens "Belgische school te Athene - École belge d'Athènes"

Tytgat Christiane, Laffineur Robert, De Wulf Alain, Hennau Marc, Goossens Rudi, Van Damme Daniel. Report on the activity of the Belgian School at Athens "Belgische school te Athene - École belge d'Athènes". In: Revue belge de philologie et d'histoire, tome 84, fasc. 1, 2006. Antiquité - Oudheid. pp. 107-122

Photogrammetric restitution of a presumed ancient Asclepius temple in Titani, Peloponnesos, Greece

Close range photogrammetry is a useful tool for the documentation and registration of archaeological sites. In this case, photogrammetric restitution is applied to a presumed Esclepion Classical temple site in Titani, Peloponnesos, Greece. The archaeological remains that are recorded and processed in this stage are small fragments of walls, made out of irregular shaped stones. The fragmentary remains and the need to record both the facades of the stones as well as the upper surfaces, complicate the photogrammetric recording and processing workflow. The use of 3D documentation is important for the documentation, conservation and possible further excavation of the site. Stereographic pictures in combination with terrestrial topographic measurements are processed in the photogrammetric software VirtuoZoTM. The stereo photographs were taken by a non-metric high resolution digital single lens reflex camera with a minimum overlap of 65 percent. Targets placed on the remains of the walls were measured by total station to obtain ground control points for the orientation of each 3D stereo model in an absolute coordinate system (HGRS87). The photogrammetric processing of the stereo models results in very accurate digital elevation models and orthophotos of the walls. Further combining of these final products and merging these products in a CAD software leads to a 3D presentation of the archaeological excavation, which can be further used to evolve this archaeological site

Characterization of Amm VIII from Androctonus mauretanicus mauretanicus: a new scorpion toxin that discriminates between neuronal and skeletal sodium channels.

The venom of the scorpion Androctonus mauretanicus mauretanicus was screened by use of a specific serum directed against AaH II, the scorpion alpha-toxin of reference, with the aim of identifying new analogues. This led to the isolation of Amm VIII (7382.57 Da), which gave a highly positive response in ELISA, but was totally devoid of toxicity when injected subcutaneously into mice. In voltage-clamp experiments with rat brain type II Na+ channel rNa(v)1.2 or rat skeletal muscle Na+ channel rNa(v)1.4, expressed in Xenopus oocytes, the EC50 values of the toxin-induced slowing of inactivation were: 29+/-5 and 416+/-14 nM respectively for AmmVIII and 2.6+/-0.3 nM and 2.2+/-0.2 nM, respectively, for AaH II interactions. Accordingly, Amm VIII clearly discriminates neuronal versus muscular Na+ channel. The Amm VIII cDNA was amplified from a venom gland cDNA library and its oligonucleotide sequence determined. It shows 87% sequence homology with AaH II, but carries an unusual extension at its C-terminal end, consisting of an additional Asp due to a point mutation in the cDNA penultimate codon. We hypothesized that this extra amino acid residue could induce steric hindrance and dramatically reduce recognition of the target by Amm VIII. We constructed a model of Amm VIII based on the X-ray structure of AaH II to clarify this point. Molecular modelling showed that this C-terminal extension does not lead to an overall conformational change in Amm VIII, but drastically modifies the charge repartition and, consequently, the electrostatic dipole moment of the molecule. At last, liquid-phase radioimmunassays with poly- and monoclonal anti-(AaH II) antibodies showed the loss of conformational epitopes between AaH II and Amm VIII

Green mamba peptide targets type-2 vasopressin receptor against polycystic kidney disease

International audiencePolycystic kidney diseases (PKDs) are genetic disorders that can cause renal failure and death in children and adults. Lowering cAMP in cystic tissues through the inhibition of the type-2 vasopressin receptor (V2R) constitutes a validated strategy to reduce disease progression. We identified a peptide from green mamba venom that exhibits nanomolar affinity for the V2R without any activity on 155 other G-protein-coupled receptors or on 15 ionic channels. Mambaquaretin-1 is a full antagonist of the V2R activation pathways studied: cAMP production, beta-arrestin interaction, and MAP kinase activity. This peptide adopts the Kunitz fold known to mostly act on potassium channels and serine proteases. Mambaquaretin-1 interacts selectively with the V2R through its first loop, in the same manner that aprotinin inhibits trypsin. Injected in mice, mambaquaretin-1 increases in a dose-dependent manner urine outflow with concomitant reduction of urine osmolality, indicating a purely aquaretic effect associated with the in vivo blockade of V2R. CD1-pcy/pcy mice, a juvenile model of PKD, daily treated with 13 [Formula: see text]g of mambaquaretin-1 for 99 d, developed less abundant (by 33%) and smaller (by 47%) cysts than control mice. Neither tachyphylaxis nor apparent toxicity has been noted. Mambaquaretin-1 represents a promising therapeutic agent against PKDs