15 research outputs found
In vitro morphogenesis in wheat/ Thinopyrum elongatum chromosome addition lines
The influence of individual
Thinopyrum elongatum
chromosomes on callus induction and morphogenic ability of
in vitro
cultivated immature embryos of wheat/
Thinopyrum elongatum
addition lines was studied. An euploid form of cultivar Chinese Spring of
Triticum aestivum
(2n=42), AABBDD, seven disomic addition lines of Chinese Spring with chromosomes of
Thinopyrum elongatum
(2n=14) EE, and the amphidiploid Chinese Spring/
Thinopyrum elongatum
(2n=56), AABBDDEE, were used as donor plants. Immature 14 days old embryos were
in vitro
cultivated. A relatively high number of calli were issued in embryocultures of all studied lines without significant variations among them. Relatively lower callus induction rate occurred only in the addition line containing 5E chromosome. Substantial differences were observed between the lines in respect to the number of both initiated morphogenic calli and regenerants obtained. The highest number of morphogenic calli and regenerants was obtained in cultures of lines containing the
Thinopyrum elongatum
chromosomes 1E and 6E and the lowest one — in the 4E addition line. The data suggest that the effect of
Thinopyrum elongatum
chromosomes could be due to existence of homeoalleles in those chromosomes, but the presence in
Thinopyrum elongatum
genome of genes and alleles affecting specifically
in vitro
response can not be excluded either