Spartan Daily, October 27, 1964

Volume 52, Issue 25https://scholarworks.sjsu.edu/spartandaily/4635/thumbnail.jp

Transcriptome-pathology correlation identifies interplay between TDP-43 and the expression of its kinase CK1E in sporadic ALS.

Sporadic amyotrophic lateral sclerosis (sALS) is the most common form of ALS, however, the molecular mechanisms underlying cellular damage and motor neuron degeneration remain elusive. To identify molecular signatures of sALS we performed genome-wide expression profiling in laser capture microdissection-enriched surviving motor neurons (MNs) from lumbar spinal cords of sALS patients with rostral onset and caudal progression. After correcting for immunological background, we discover a highly specific gene expression signature for sALS that is associated with phosphorylated TDP-43 (pTDP-43) pathology. Transcriptome-pathology correlation identified casein kinase 1ε (CSNK1E) mRNA as tightly correlated to levels of pTDP-43 in sALS patients. Enhanced crosslinking and immunoprecipitation in human sALS patient- and healthy control-derived frontal cortex, revealed that TDP-43 binds directly to and regulates the expression of CSNK1E mRNA. Additionally, we were able to show that pTDP-43 itself binds RNA. CK1E, the protein product of CSNK1E, in turn interacts with TDP-43 and promotes cytoplasmic accumulation of pTDP-43 in human stem-cell-derived MNs. Pathological TDP-43 phosphorylation is therefore, reciprocally regulated by CK1E activity and TDP-43 RNA binding. Our framework of transcriptome-pathology correlations identifies candidate genes with relevance to novel mechanisms of neurodegeneration

Effects of amoxicillin repeated administration on the hemogram and biogram of sheep

Background: The object of the present study was to investigate the possible alterations in hematological and biochemical parameters of sheep that may occur following intramuscular injection of amoxicillin.Methods: Amoxicillin was injected to 10 sheep at a dosage regimen of 7 mg/kg of body weight for 5 successive days. Two types of blood samples (with and without ethylenediaminetetraacetic acid as an anticoagulant) were collected from the jugular vein before and after the antibiotic course.Results: Amoxicillin significantly (p<0.001) increased total leukocyte count and (p<0.05) absolute eosinophilic count when compared with those of the control samples. Aspartate aminotransferase, alkaline phosphatase and cholesterol, were significantly (p<0.05) higher than the corresponding control values. In addition, amoxicillin significantly (p<0.05) increased blood urea nitrogen and creatinine but decreased phosphorus level when compared with those of prior-administration samples.Conclusions: These data may suggest that although the side changes caused by amoxicillin are minor in sheep, yet the liver and kidney functions should be monitored during its usage in therapy and it should be used with care for treatment of sheep with renal and/or hepatic impairments; its dosage regimen should be adjusted to avoid its hepatotoxic and nephrotoxic effects

Ocular morbidities of premature children with mild or no retinopathy of prematurity

SUMMARY: Küçükevcilioğlu M, Mutlu FM, Sarıcı SÜ. Ocular morbidities of premature children with mild or no retinopathy of prematurity. Turk J Pediatr 2015; 57: 129-135. This study reports the ocular and neurologic outcomes at 3 years of age of preterm children with mild or no retinopathy of prematurity (ROP). We were able to compile data from the medical records of 119 out of 585 (20.3%) children with mild and no ROP, who were screened between March 1999 and March 2012. There were 52 children with mild ROP and 67 with no ROP. In terms of ROP-related risk factors, the mild ROP and no ROP groups showed significant differences in birth weight (p<0.001) and gestational age (p<0.001), as well as in the presence of mechanical ventilation (p=0.04), respiratory distress syndrome (p=0.003), blood transfusion (p=0.006) and sepsis (p=0.024). However, both groups seemed identical in terms of the distribution of refractive errors, presence of strabismus, presence of unfavorable structural or functional outcome, and presence of neurologic sequelae. In particular, the high incidence of neurologic morbidity (100%) in subjects with very poor vision suggested a clear correlation

Patterns of medication use and factors associated with antibiotic use among adult fever patients at Singapore primary care clinics.

BACKGROUND: Antimicrobial resistance is a public health problem of global importance. In Singapore, much focus has been given to antibiotic usage patterns in hospital settings. Data on antibiotic use in primary care is lacking. We describe antibiotic usage patterns and assess factors contributing to antibiotic usage among adults presenting with acute febrile illness (AFI) in primary care settings in Singapore. METHODS: We analyzed data from the Early Dengue infection and outcome study. Adults with AFI presenting at 5 Singapore polyclinics were included. We used multivariable logistic regression to assess demographic, clinical and laboratory factors associated with antibiotic usage among adults with AFI. RESULTS: Between December 2007 and February 2013, 1884 adult AFI patients were enrolled. Overall, 16% of adult AFI patients reported antibiotic use. We observed a rise in the use of over-the-counter medications in late 2009 and a decrease in antibiotic use during 2010, possibly related to the outbreak of pandemic influenza A H1N1 virus. After adjusting for age, gender, polyclinic and year of enrolment, the following factors were associated with higher odds of antibiotic use: living in landed property (compared to public housing) (OR = 1.73; 95% CI: 1.06-2.80); body mass index (BMI) <18.5 (OR = 1.87; 95% CI: 1.19-2.93); elevated white blood cell (WBC) count (OR = 1.98; 95% CI: 1.42-2.78); and persistence of initial symptoms at 2-3 days follow-up with OR (95% CI) for categories of 1, 2, 3, and ≥4 persisting symptoms being 2.00 (1.38-2.92), 2.67 (1.80-3.97), 4.26 (2.73-6.64), and 2.79 (1.84-4.24) respectively. CONCLUSIONS: Our study provides insights on antibiotic usage among adult patients presenting to primary care clinics with febrile illness, and suggests that high socio-economic status, and risk factors of a severe illness, that is, low BMI and persistence of initial symptoms, are associated with higher antibiotic use. Further work to understand trends of antibiotic usage in both private and public primary care clinics, and factors that influence patient expectation and physician prescribing of antibiotics is important

On Bitter-Juicy Philosophizing Via Aesthetics

To philosophize or not is a matter of conscious choice and option. But when we start with such a premise, we have to lay down what we understand by this activity. In the paper the author undertakes this task, distinguishing four-fold the philosophizing practice with regard to the domain of aesthetics. In the final section of the paper he considers the problem which seems to him fundamental, namely why today philosophizing via aesthetics in a definite way should be recommendable and primary, as well as why it has to be bitter-juicy.To philosophize or not is a matter of conscious choice and option. But when we start with such a premise, we have to lay down what we understand by this activity. In the paper the author undertakes this task, distinguishing four-fold the philosophizing practice with regard to the domain of aesthetics. In the final section of the paper he considers the problem which seems to him fundamental, namely why today philosophizing via aesthetics in a definite way should be recommendable and primary, as well as why it has to be bitter-juicy

Spartan Daily, January 25, 1954

Volume 42, Issue 71https://scholarworks.sjsu.edu/spartandaily/11966/thumbnail.jp