Chemo-Photothermal Combination Therapy of HER-2 Overexpressing Breast Cancer Cells with Dual-Ordered Mesoporous Carbon@Silica Nanocomposite

In cancer treatment, the complexity of tumors seriously affects the therapeutic potential of the treatment. Treatments with combination therapy result in more potent effects than monotherapy or their theoretical combination in cancer treatment. Photothermal therapy (PTT) includes applying phototherapeutic agents that cause local hyperthermia responsible for the thermal ablation of tumor cells after applying near-infrared light and is often applied with other combination therapies. In this study, the chemo-PTT potential of synthesized drug-loaded and targeted GEM/TRA-MC@Si nanocomposite on Her2 positive breast cancer cell line (SK-BR-3) and human triple-negative breast cancer cell line (MDA-MB-231) was investigated using NIR application as in vitro. First, the cell viability (IC50) value of the GEM/TRA-MC@Si nanocomposite was determined as 25 mu g/mu L. Then, chemo-PTT was performed, and the viability of the cells was evaluated. In addition, the live/dead cell rate was established by staining with the Calcein-AM and EthD-1, and apoptosis tests were completed. When the surface temperature of Her2 positive SK-BR-3 cells exceeded 47 degrees C during PTT with an irradiation time of > 100 s, it caused cell death. In this study, it was demonstrated that in vitro PTT (1 W/cm(2), 180 s) was applied using GEM/TRA-MC@Si nanocomposite (25 mu g/mL) on her2 + SK-BR-3 cell line, which contributed to the reduction of cell viability. In addition, this study demonstrates that chemo-PTT with targeted GEM/TRA-MC@Si nanocomposite induced SK-BR-3 cell viability and can initiate cell death through the apoptosis pathway under optimized irradiation conditions. Herewith chemo-PTT combination therapy of targeted GEM-TRA/MC@Si nanocomposite was found to be effective on SK-BR-3 cells as in vitro.Ege University Scientific Research Projects (BAP) Coordinator ship [FDK-2020-21380]; Scientific and Technical Research Council of Turkey (TUBITAK) [2211-C, 2214-A, 1649B031802269, 1059B141801597]Financial support was provided by Ege University Scientific Research Projects (BAP) Coordinator ship (Project Number: FDK-2020-21380) and the Scientific and Technical Research Council of Turkey (TUBITAK) with 2211-C and 2214-A coded doctoral research support (grant number, respectively: 1649B031802269 and 1059B141801597)

Docetaxel loaded human serum albumin nanoparticles; synthesis, characterization, and potential of nuclear imaging of prostate cancer

Tuncel, Ayca/0000-0003-0699-3309WOS: 000518386500059In this study, docetaxel loaded nanoparticles were synthesized and labeled with I-131 for diagnosis and treatment of prostate cancer. Docetaxel (DTX) was loaded onto human serum albumin nanoparticles (HSA) with 50% efficiency. the size of DTX loaded nanoparticles (DTX-HSA) is in the range of 150-160 nm and these nanoparticles have a zeta potential of - 27.2 mV. in in vitro drug release assay, 80% of DTX from loaded DTX-HSA nanoparticles was released at pH 7.4 medium, while 93% of DTX was released from DTX-HSA nanoparticles at pH 5.8 medium at the end of 48 h. the data show that HSA nanoparticles might be increasing in chemotherapy effect as a drug delivery system. DTX-HSA nanoparticles were labeled with I-131 via iodogen method. Intracellular uptake experiments of I-131 -DTX-HSA nanoparticles were done on prostate tumor cells (PC-3) and healthy prostate cells (RWPE-1) at 24 h. in conclusion, I-131 -DTX-HSA appears as suitable agent that might be used in both nuclear imaging and radiotherapy of prostate cancer. in this study, the nuclear imaging potential of I-131-DTX-HSA was determined as in vitro. the results of this study are important to investigate this potential with in vivo studies in the future

Synthesis and Optimization of the Docetaxel-Loaded and Durvalumab-Targeted Human Serum Albumin Nanoparticles, In Vitro Characterization on Triple-Negative Breast Cancer Cells

Triple-negative breastcancer (TNBC) tends to behave more aggressivelycompared to other breast cancer subtypes due to the lack of receptorsand its limited targeting therapy. In recent years, nanotechnologyadvancement has led to the development of various nanoparticle platformsfor the targeted treatment of cancers. Especially, HSA-NPs have specificadvantages such as biocompatibility, adjustable size during production,and relatively easy synthesis. In this study, HSA-NPs were encapsulatedwith docetaxel (DTX) and functionalized with polyethylene glycol (PEG),also becoming a targeting nanoplatform modified with durvalumab (DVL),and the whole nanostructure was well characterized. Subsequently,drug release studies and various in vitro cell culturestudies such as determining the cytotoxicity and apoptotic levelsof the nanoplatforms and PD-L1 using ELISA test were conducted onMDA-MB-468, MDA-MB-231, and MCF-7 cells. According to the results,HSA-DTX@PEG-DVL NPs showed better cytotoxicity compared to DTX inall the three cell lines. In addition, it was observed that the HSA-DTX@PEG-DVLNPs did not lead the cells to late apoptosis but were effective inthe early apoptotic stage. Moreover, the ELISA data showed a significantlyinduced PD-L1 expression due to the presence of DVL in the nanostructure,which indicates that DVL antibodies successfully bind to the [email protected] work was supported by Ege University Scientific Research Projects (ONAP) Coordinator ship (project number: FOA-2020-22274).Ege University Scientific Research Projects (ONAP) Coordinator ship [FOA-2020-22274

Do we protect the pelvic floor with non-elective cesarean? A study of 3-D/4-D pelvic floor ultrasound immediately after delivery

WOS: 000333615200020PubMed: 24612399AimTo compare levator defect, loss of tenting, change in biometric measurements of the levator ani and genital hiatus according to the mode of delivery, length of the labor, Bishop score, birthweight and head circumference immediately after delivery. MethodsOne hundred and seventy-one primiparous women who delivered either by vaginal delivery or cesarean were prospectively evaluated. Two 3-D volumes (one at rest, one on Valsalva maneuver) were recorded in the supine position after voiding, and levator biometry, levator defect and loss of tenting were determined on the axial plane. ResultsOf 171 nulliparous women, 84 had vaginal delivery and 87 had cesarean delivery. All hiatal dimensions on resting and maximal Valsalva were found to be higher in the vaginal delivery group. Levator defect rate was found to be significantly higher in the vaginal delivery group (P<0.0001). We found a positive correlation with head circumference, fetal weight and first stage labor length in women who delivered vaginally. In the cesarean delivery group, mean fetal head circumference, fetal weight, length of first stage of labor and Bishop score were higher in women with levator ani defect. Loss of tenting rate was significantly higher in vaginal delivery women (P=0.03). ConclusionLabor itself, and factors such as fetal head circumference and fetal weight that cause prolongation of labor, can induce levator ani muscle defect or microtrauma which in turn can cause morphological alterations of the levator hiatus

Nuclear imaging potential and in vitro photodynamic activity of Boron subphthalocyanine on colon carcinoma cells

Biyiklioglu, Zekeriya/0000-0001-5138-214X; Tuncel, Ayca/0000-0003-0699-3309WOS: 000532688100010Photodynamic therapy (PDT) has been a promising clinical agent in various types of cancer in recent years. in this study, in vitro nuclear imaging and PDT potential of Es-SubPc (Boron subphthalocyanine) were evaluated in colon adenocarcinoma cell line (HT-29) and human healthy lung fibroblast cell line (WI-38). For this purpose, the Es-SubPc was labeled with I-131 using the iodogen method under the optimum conditions resulting in labeled with high yield (98.9 +/- 1.2%). in addition, the uptake rate of I-131-Es-SubPc was determined in HT-29 and WI-38 cell lines. in comparison to the healthy cell line, the uptake of 131I-Es-SubPc was found to be 2-fold higher in the HT-29 cell line. For PDT studies, both cells were exposed to white light at 30-90 J/cm(2) in the presence of Es-SubPc. the results showed that Es-SubPc was a good PDT agent likely to be used in HT-29 cell line. As a result, Es-SubPc can be promising nuclear imaging and PDT agent for colon carcinoma

Antifungal photodynamic activities of phthalocyanine derivatives on Candida albicans

Biyiklioglu, Zekeriya/0000-0001-5138-214X; Tuncel, Ayca/0000-0003-0699-3309WOS: 000540895900057PubMed: 32165338Antimicrobial resistance is one of the most important causes of morbidity and mortality in the treatment of infectious diseases worldwide. Candida albicans is one of the most virulent and common species of fungi to cause invasive fungal infections on humans. Alternative treatment strategies, including photodynamic therapy, are needed for controlling these infectious diseases. the aim of this study was to investigate the antifungal photodynamic activities of phthalocyanine derivatives on C. albicans. the minimum inhibitory concentration (MIC) values of compounds were determined by the broth microdilution method. Uptake of the compounds in C. albicans and dark toxicity of the compounds were also investigated. Photodynamic inhibition of growth experiments was performed by measuring the colony-forming unit/mL (CFU/mL) of the strain. Maximum uptake into the cells was observed in the presence of 64 mu g/mL concentration for each compound except for ZnPc. Compounds did not show dark toxicity/inhibitory effects at sub-MIC concentrations on C. albicans when compared to the negative control groups. Zn(II)Pc, ZnPc, and ZnPc-TiO2 showed fungicidal effect after irradiation with the light dose of 90 J/cm(2) in the presence of the compounds. in addition to the fungicidal effects, SubPc, SubPc-TiO2, Es-SiPc, and Es-SubPc compounds were also found to have inhibitory effects on the growth of yeast cells after irradiation.Ege University Scientific Research Projects (BAP) commission [18 NBE 003]This work was supported by Ege University Scientific Research Projects (BAP) commission with 18 NBE 003 project code

Antimicrobial photodynamic therapy against Staphylococcus aureus using zinc phthalocyanine and zinc phthalocyanine integrated TiO2 nanoparticles

WOS: 000460467300022Antibiotic resistance is an increasing healthcare problem worldwide. In the present study, the effects of antimicrobial photodynamic therapy (APDT) of ZnPc and ZnPc-integrated TiO2 nanoparticles (ZnPc-TiO2) were investigated against Staphylococcus aureus. A light emitting diode (LED) (630-700 nm, 17.4 mW/cm(2)) was used on S. aureus at different light doses (8 J/cm(2) for 11 min, 16 J/cm(2) for 22 min, 24 J/cm(2) for 33 min) in the presence of the compounds under the minimum inhibitory concentration values. Both compounds showed similar phototoxicity toward S. aureus when high light doses (16 and 24 J/cm(2)) were applied. In addition, the success of APDT increased with an increasing light dose.Ege University, Scientific Research Project (BAP)Ege University [18NBE003]This research was supported by the Ege University, Scientific Research Project (BAP), Project Number: 18NBE003

Evaluation of infection imaging potential of I-131-labeled imidazolium salt

WOS: 000426219900006Effective antimicrobial compounds are necessary due to increased resistance of antibiotics against microorganisms causing infectious diseases. In this study, imidazolium-TFSI salt [ITFSI: octyl-bis(3-methylimidazolium)-di(bis(trifluoromethane)sulfonimide)] was labeled with I-131 with high efficiency. In vitro uptake experiments of I-131-ITFSI showed high uptake in gram-positive Staphylococcus aureus bacteria. I-131-ITFSI was also evaluated for comparison between bacterial infection and sterile inflammation by in vivo studies. The biodistribution results revealed that I-131-ITFSI might be used as a nuclear imaging agent for detection of bacterial infection