27 research outputs found
Intracellular delivery of messenger RNA by recombinant PP7 virus-like particles carrying low molecular weight protamine
Full text linkReplacement of conserved or variable sequences of the mosquito-borne dengue virus 3′ UTR with homologous sequences from Modoc virus does not change infectivity for mosquitoes
No full textA proof-of-concept study for the design of a VLP-based combinatorial HPV and placental malaria vaccine
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Reevaluation of Epidemiological Data Demonstrates That It Is Consistent With Cross-Immunity Among Human Papillomavirus Types
No full textCombination of human papillomaviruses L1 and L2 multiepitope constructs protects mice against tumor cells
No full textIdentification of Broad-Genotype HPV L2 Neutralization Site for Pan-HPV Vaccine Development by a Cross-Neutralizing Antibody
No full textVLPs Displaying a Single L2 Epitope Induce Broadly Cross-Neutralizing Antibodies against Human Papillomavirus
No full textA Vaccine of L2 Epitope Repeats Fused with a Modified IgG1 Fc Induced Cross-Neutralizing Antibodies and Protective Immunity against Divergent Human Papillomavirus Types
No full textReplacement of the 3′ untranslated variable region of mosquito-borne dengue virus with that of tick-borne Langat virus does not alter vector specificity
No full textThe four major flavivirus clades are transmitted by mosquitoes, ticks, directly between vertebrates or directly between arthropods, respectively, but the molecular determinants of mode of transmission in flaviviruses are unknown. To assess the role of the UTRs in transmission, we generated chimeric genomes in which the 5′ UTR, capsid and/or 3′ UTR of mosquito-borne dengue virus serotype 4 (rDENV-4) were replaced, separately or in combination, with those of tick-borne Langat virus (rLGTV). None of the chimeric genomes yielded detectable virus following transfection. Replacement of the variable region (VR) in the rDENV-4 3′ UTR with that of rLGTV generated virus rDENV-4-rLGTswapVR, which showed lower replication than its wild-type parents in mammalian but not mosquito cells in culture and was able to infect mosquitoes in vivo. Neither rDENV-4 nor rDENV-4-rLGTswapVR could infect larval Ixodes scapularis ticks immersed in virus, while rLGTV was highly infectious via this route
