Rouleaux red blood cells splitting in microscopic thin blood smear images via local maxima, circles drawing, and mapping with original RBCs.

Splitting the rouleaux RBCs from single RBCs and its further subdivision is a challenging area in computer-assisted diagnosis of blood. This phenomenon is applied in complete blood count, anemia, leukemia, and malaria tests. Several automated techniques are reported in the state of art for this task but face either under or over splitting problems. The current research presents a novel approach to split Rouleaux red blood cells (chains of RBCs) precisely, which are frequently observed in the thin blood smear images. Accordingly, this research address the rouleaux splitting problem in a realistic, efficient and automated way by considering the distance transform and local maxima of the rouleaux RBCs. Rouleaux RBCs are splitted by taking their local maxima as the centres to draw circles by mid-point circle algorithm. The resulting circles are further mapped with single RBC in Rouleaux to preserve its original shape. The results of the proposed approach on standard data set are presented and analyzed statistically by achieving an average recall of 0.059, an average precision of 0.067 and F-measure 0.063 are achieved through ground truth with visual inspection

Albumin test strip for quick detection of albuminuria in human

496-498In this article, the dry -reagent test strip technique has been discussed for qualitative and semi-quantitative estimation of albumin in urine. The strip method developed in our laboratory is quick, simple, economical and based on indigenous technique. It is based on the principle of ‘‘Protein error’’ in which specific chromogen immobilized onto a pad reacts with albumin present in the urine and changes the colour of the strips from light yellow to bluegreen. The change in colour is visible to the naked eyes and can be compared to the colour chart for the <span style="font-size: 14.0pt;font-family:" times="" new="" roman";mso-fareast-font-family:hiddenhorzocr;="" color:black;mso-bidi-font-weight:bold"="" lang="EN-IN">estimation of total albumin concentration present in the urine sample. </span

Arsenazo III test strip for rapid detection of hardness of water

252-254In this article development and optimization of visually evaluable dry-reagent test strip technique for qualitative and semi-quantitative estimation of hardness of water has been described. The hardness of water is based on its alkaline earth content i.e. calcium and magnesium ions and their salts. The estimation of hardness of water in industry as well as in private sector is essential. Presently, calorimetric assay and test kit methods are commonly used, however, they are expensive and require trained persons to perform the test. The dry reagent strip developed in the laboratory is quick, simple and economical. It is based on specific chromogen immobilized on to a pad which reacts with calcium present in water and thereby changes the colour of the strip from purple to blackish blue. The change in colour is visible with naked eyes and can be compared with the colour chart