Subclinical immune reactions to viral infections may correlate with child and adolescent diagnosis of attention-deficit/hyperactivity disorder: A preliminary study from Turkey

Background: Attention-Deficit/Hyperactivity Disorder (ADHD) is one of the most common neuro-developmental disorders of childhood and  adolescence. Studies focusing on the relationship of infectious agents and ADHD are scarce. It is also known that cerebellar injury may lead to  hyperactive behavior. This study aimed to evaluate the relationship  between viral agents of cerebellitis and the diagnosis of ADHD.Methods: The study group was formed of 60 consecutive ADHD patients and 30 healthy children. IgG levels for VZV; HSV-1, CMV, Measles, Mumps, Rubella and EBV were evaluated.Results: Males were significantly higher among patients with ADHD (65% vs. 40%, p=0.025). Patients with ADHD displayed significantly higher positivity for measles IgG (80% vs. 60%, p=0.044). When patients with ADHD were classified according to their pubertal status, adolescents with ADHD displayed higher positivity for mumps (100% vs. 74.4%, p=0.043). Most of the patients were diagnosed with ADHD-Combined or  Hyperactive/Impulsive Subtypes (56.6%) while 43.3% were diagnosed with ADHD-predominantly inattentive type. When patients with subtypes of ADHD were compared in terms of seropositivity, it was found that patients with ADHD-Combined/ Hyperactive-Impulsive subtypes had significantly elevated reactions for Rubella (100% vs. 88.5%, p=0.044).Conclusion: Although limited to a single center and may be prone to sampling biases, our results may support the notion that immune reactions may be related with ADHD among children and adolescents. Further,  prospective studies from multiple centers are needed to support our  findings and establish causality.Key words: ADHD, infection, immunology, measles, rubella, mumps, Ig

Susac’s Syndrome

We have read the interesting article by Raets and Gelin, entitled “Susac’s syndrome: a clinical and radiological challenge” which was published in the 95 (6) issue of the Journal in 2012 (1). The article included fairly important information which was very useful for us. However, we would like to make a few contributions

Electronic and magnetic properties of the topological semimetal SmMg2_2Bi2_2

Dirac semimetals show nontrivial physical properties and can host exotic quantum states like Weyl semimetals and topological insulators under suitable external conditions. Here, by combining angle-resolved photoemission spectroscopy measurements (ARPES) and first-principle calculations, we demonstrate that Zintl-phase compound SmMg$_2$Bi$_2$ belongs to the close proximity to a topological Dirac semimetallic state. ARPES results show a Dirac-like band crossing at the zone-center near the Fermi level ($E_\mathrm {F}$) which is further confirmed by first-principle calculations. Theoretical studies also reveal that SmMg$_2$Bi$_2$ belongs to a $Z_2$ topological class and hosts spin-polarized states around the $E_\mathrm {F}$. Zintl's theory predicts that the valence state of Sm in this material should be Sm$^{2+}$, however we detect many Sm-4$f$ multiplet states (flat-bands) whose energy positions suggest the presence of both Sm$^{2+}$ and Sm$^{3+}$. It is also evident that these flat-bands and other dispersive states are strongly hybridized when they cross each other. Due to the presence of Sm$^{3+}$ ions, the temperature dependence of magnetic susceptibility $\chi(T)$ shows Curie-Weiss-like contribution in the low temperature region, in addition to the Van Vleck-like behaviour expected for the Sm$^{2+}$ ions. The present study will help in better understanding of the electronic structure, magnetism and transport properties of related materials.Comment: 10 pages, 7 figure

Anisotropic magnetism and electronic structure of trigonal EuAl2_2Ge2_2 single crystals

The magnetic and electronic properties of the layered Zintl-phase compound EuAl$_2$Ge$_2$ crystallizing in the trigonal CaAl$_2$Si$_2$-type structure are reported. Our neutron-diffraction measurements show that EuAl$_2$Ge$_2$ undergoes A-type antiferromagnetic (AFM) ordering below $T_{\rm N} = 27.5(5)$~K, with the Eu moments (Eu$^{2+}$, $S = 7/2$) aligned ferromagnetically in the $ab$ plane. The $H = 0$ magnetic structure consists of trigonal AFM domains associated with $ab$-plane magnetic anisotropy and a field-induced reorientation of the Eu spins in the domains is evident at $T = 2$~K below the critical field $H_{c1} = 2.5(1)$ kOe. Electrical resistivity and ARPES measurements show that EuAl$_2$Ge$_2$ is metallic both above and below $T_{\rm N}$. In the AFM phase, we directly observe folded bands in ARPES due to the doubling of the magnetic unit cell along the $c$ axis with an enhancement of quasiparticle weight due to the complex change in the coupling between the magnetic moments and itinerant electrons on cooling below $T_{\rm N}$. The observed electronic structure is well reproduced by first-principle calculations, which also predict the presence of nontrivial electronic states near the Fermi level in the AFM phase with $Z_2$ topological numbers 1;(000).Comment: 16 pages, 13 captioned figures, 53 references Updated several affiliation

Atmospheric Pressure Mass Spectrometry of Single Viruses and Nanoparticles by Nanoelectromechanical Systems

Mass spectrometry of intact nanoparticles and viruses can serve as a potent characterization tool for material science and biophysics. Inaccessible by widespread commercial techniques, the mass of single nanoparticles and viruses (>10MDa) can be readily measured by NEMS (Nanoelectromechanical Systems) based Mass Spectrometry, where charged and isolated analyte particles are generated by Electrospray Ionization (ESI) in air and transported onto the NEMS resonator for capture and detection. However, the applicability of NEMS as a practical solution is hindered by their miniscule surface area, which results in poor limit-of-detection and low capture efficiency values. Another hindrance is the necessity to house the NEMS inside complex vacuum systems, which is required in part to focus analytes towards the miniscule detection surface of the NEMS. Here, we overcome both limitations by integrating an ion lens onto the NEMS chip. The ion lens is composed of a polymer layer, which charges up by receiving part of the ions incoming from the ESI tip and consequently starts to focus the analytes towards an open window aligned with the active area of the NEMS electrostatically. With this integrated system, we have detected the mass of gold and polystyrene nanoparticles under ambient conditions and with two orders-of-magnitude improvement in capture efficiency compared to the state-of-the-art. We then applied this technology to obtain the mass spectrum of SARS-CoV-2 and BoHV-1 virions. With the increase in analytical throughput, the simplicity of the overall setup and the operation capability under ambient conditions, the technique demonstrates that NEMS Mass Spectrometry can be deployed for mass detection of engineered nanoparticles and biological samples efficiently.Comment: 38 pages, 6 figure

Estimates for heritability and consumer-validation of a penetrometer method for phenotyping softness of cooked cassava roots

Although breeders have made significant progress in the genetic improvement of cassava ( Manihot esculenta Crantz) for agronomic traits, lack of information on heritability and limited testing of high-throughput phenotyping methods are major limitations to improving root quality traits, such as softness after cooking, which rank high among Ugandan consumers. The objectives of this study were to determine heritability for softness of cooked cassava roots, and quantify the relationship between penetrometer and consumer testing methods for phenotyping softness of cassava roots. Softness defined as the maximum force (N) needed to penetrate cooked root samples using a penetrometer, was evaluated at four cooking time intervals: 15, 30, 45, and 60 min on 268 cassava genotypes. Estimates of broad-sense heritability (repeatability) ranged from 0.17 to 0.37, with the highest value observed at 45 min of cooking time interval. In the second study involving 135 cassava consumers from Kibaale district in Uganda, penetrometer measurements of cooked roots from six cassava varieties were found to be in strong agreement (r2 = 0.91; P-value = 0.003) with ordinal scores of root softness from consumer testing. These results suggest that: (a) softness of cooked cassava roots is a trait amenable for evaluation and selection; and (b) a penetrometer can readily be used for assessment of cooked root softness. These findings form the basis for operationalising the routine assessment of root softness in cassava breeding trials, an output that will enhance ongoing efforts to breed for desired end-user root quality traits.Les s\ue9lectionneurs ont fait des progr\ue8s consid\ue9rables dans l\u2019am\ue9lioration g\ue9n\ue9tique des caract\ue8res agronomiques du manioc ( Manihot esculenta Crantz). Cependant un manque de m\ue9thodes de ph\ue9notypage haut-d\ue9bit adapt\ue9es aux caract\ue8res de qualit\ue9s tel que la fermet\ue9 de la racine apr\ue8s cuisson, essentiel pour les consommateurs Ougandais. Cette \ue9tude ambitionne a) de determiner l\u2019h\ue9ritabilit\ue9 de la fermete de racine cuite b) de quantifier la relation entre l \ue9valuation de la fermet\ue9 de racine cuite au p\ue9n\ue9trom\ue8tre et un panel consommateurs. Cette \ue9valuation a \ue9t\ue9 realisee a quatre intervals de temps: 15, 30, 45 et 60 minutes sur 268 genotypes de manioc. Pour d\ue9finir l\u2019h\ue9ritabilit\ue9 de la fermet\ue9 de la racine, celle ci a \ue9t\ue9 d\ue9finie par la force maximum (N) n\ue9cessaire pour p\ue9n\ue9trer des \ue9chantillons de racines incluant diff\ue8rent temps de cuisson (15, 30, 45, and 60 min) a l\u2019aide d\u2019un p\ue9n\ue9trom\ue8tre. L\u2019h\ue9ritabilit\ue9 au sens large (ou r\ue9p\ue9tabilit\ue9) observ\ue9e varie de 0.17 a 0.37, la valeur la plus \ue9lev\ue9 \ue9tant observ\ue9e pour un temps de cuisson de 45 minutes. Dans une seconde \ue9tude impliquant 135 consommateurs du district de Kibaale (Ouest de l\u2019Ouganda), les mesures au p\ue9n\ue9trom\ue8tre de racines cuites de six vari\ue9t\ue9s ont confirm\ue9es la forte correlation (r2 = 0.91; P-value = 0.003) avec les valeurs ordinales de fermet\ue9 de racine du panel consommateur. Les r\ue9sultats de cette \ue9tude indiquent que cette m\ue9thodologie de ph\ue9notypage est a) utile pour l\u2019 \ue9valuation de la fermet\ue9 sur des racines cuites en selection et b) d\ue9montre que l\u2019usage du p\ue9n\ue9trom\ue8tre est efficace pour celle ci. Ces r\ue9sultats offrent aux s\ue9lectionneurs une methode d\u2019 \ue9valuation de routine de la qualite de racine pour les essais experimentaux. Ceux ci contribueront aux efforts actuels pour l\u2019am\ue9lioration des caract\ue8res qualit\ue9s chers aux consommateurs