The effect of macro-control and organizational support perception on nurses and physicians intention to quit during the COVID-19 pandemic

AimThe aim of this study is to determine the effect of organizational support and COVID-19 macro-control perception levels on intention to quit in physicians and nurses. BackgroundDuring the pandemic process, healthcare workers faced great health risks and workloads. Many health workers are considering quitting their job. MethodA cross-sectional and explanatory research. The sample of the study consisted of 300 nurses and 73 physicians. To collect data, COVID-19 perception of macro-control, perceived organizational support, and intention to quit scales were used. Structural equation modeling was used. ResultsCOVID-19 macro-control perception and perceived organizational support have a negative effect on the intention to quit. The perceived organizational support has a partial mediating role, on the COVID-19 macro-control perception and the intention to quit. ConclusionThis study demonstrated that the macro measures taken against the pandemic and the organizational support received by the nurses and physicians, who are the main actors in the fight against the epidemic, negatively affected their intention to leave. Implications for nursing practice and clinical relevanceTo reduce the intention to quit health workers during the COVID-19 pandemic, scientific-based macro-control measures should be taken, and organizational support should be provided

Poly-(L)-Histidine Immobilized Cryogels for Lysozyme Purification

Immobilized metal ion affinity chromatography is one of the methods used for the adsorption of proteins. In this study, poly(glycidyl methacrylate) cryogel discs were prepared by free radical polymerization. The metal chelating groups were polymeric chain of poly-(l)-histidine (mol wt5000) having poly-imidazole ring sequence. Then, Cu(II), Zn(II), and Ni(II) ions were separately chelated on the poly-(l)-histidine immobilized poly(glycidyl methacrylate) cryogel discs to be used in immobilized metal ion affinity chromatography separation of lysozyme. The swelling test, Fourier transform infrared spectroscopy, Brunauer-Emmett-Teller, and scanning electron microscopy were performed to characterize both poly(glycidyl methacrylate) and poly-(l)-histidine immobilized poly(glycidyl methacrylate) cryogel discs. The effects of the pH, lysozyme concentration, adsorption time, and ionic strength on the adsorption capacity were studied. These parameters were varied between 4.0 and 8.0 for pH, 0.0 and 2.0mg/ml for initial lysozyme concentration, 0 and 120min for adsorption time, and 0.0 and 1.0 mu M for ionic strength. The maximum lysozyme adsorption capacity of the Cu(II), Zn(II), and Ni(II) ions chelated poly-(l)-histidine immobilized poly(glycidyl methacrylate) cryogel discs was 36.4, 26.8, and 17.3mg/g cryogel, respectively. Desorption of lysozyme from cryogel discs was easily achieved by 1.0M NaCI solution. Repeated adsorption-elution processes showed that these cryogel discs were suitable for repeatable lysozyme adsorption. Adsorption isotherms fitted to Langmuir model and adsorption kinetics suited to pseudo-second order model. Thermodynamic parameters (i.e. H degrees, S degrees, G degrees) were also calculated from Langmuir isotherms at different temperatures.WoSScopu