The DNA methylation inhibitor 5-aza-2’-deoxycytidine retards cell growth and alters gene expression in canine mammary gland tumor cells

Disruption of gene expression by DNA methylation changes is widely involved in tumorigenesis. Here,to investigate DNA methylation changes in canine, we treated a canine mammary gland tumor cell line with a DNA methylation inhibitor, 5-aza-2’-deoxycytidine (5-aza). Cell growth was significantly retarded following 5-aza treatment and the epithelial marker genes CDH1 and KRT18 were significantly up-regulated, whereas the mesenchymal marker genes CDH2 and VIM were significantly downregulated. We also found a significant decrease in DNA methylation level in the CDH1 promoter region by 5-aza treatment. These results showed for the first time in canine mammary gland tumor cells that inhibition of DNA methylation caused cell growth retardation and affected epithelial mesenchymal transition-related gene expression via changes in DNA methylation level

Approximate Span Programs

Span programs are a model of computation that have been used to design quantum algorithms, mainly in the query model. For any decision problem, there exists a span program that leads to an algorithm with optimal quantum query complexity, but finding such an algorithm is generally challenging. We consider new ways of designing quantum algorithms using span programs. We show how any span program that decides a problem $f$ can also be used to decide "property testing" versions of $f$, or more generally, approximate the span program witness size, a property of the input related to $f$. For example, using our techniques, the span program for OR, which can be used to design an optimal algorithm for the OR function, can also be used to design optimal algorithms for: threshold functions, in which we want to decide if the Hamming weight of a string is above a threshold or far below, given the promise that one of these is true; and approximate counting, in which we want to estimate the Hamming weight of the input. We achieve these results by relaxing the requirement that 1-inputs hit some target exactly in the span program, which could make design of span programs easier. We also give an exposition of span program structure, which increases the understanding of this important model. One implication is alternative algorithms for estimating the witness size when the phase gap of a certain unitary can be lower bounded. We show how to lower bound this phase gap in some cases. As applications, we give the first upper bounds in the adjacency query model on the quantum time complexity of estimating the effective resistance between $s$ and $t$, $R_{s,t}(G)$, of $\tilde O(\frac{1}{\epsilon^{3/2}}n\sqrt{R_{s,t}(G)})$, and, when $\mu$ is a lower bound on $\lambda_2(G)$, by our phase gap lower bound, we can obtain $\tilde O(\frac{1}{\epsilon}n\sqrt{R_{s,t}(G)/\mu})$, both using $O(\log n)$ space

Generating facets for the cut polytope of a graph by triangular elimination

The cut polytope of a graph arises in many fields. Although much is known about facets of the cut polytope of the complete graph, very little is known for general graphs. The study of Bell inequalities in quantum information science requires knowledge of the facets of the cut polytope of the complete bipartite graph or, more generally, the complete k-partite graph. Lifting is a central tool to prove certain inequalities are facet inducing for the cut polytope. In this paper we introduce a lifting operation, named triangular elimination, applicable to the cut polytope of a wide range of graphs. Triangular elimination is a specific combination of zero-lifting and Fourier-Motzkin elimination using the triangle inequality. We prove sufficient conditions for the triangular elimination of facet inducing inequalities to be facet inducing. The proof is based on a variation of the lifting lemma adapted to general graphs. The result can be used to derive facet inducing inequalities of the cut polytope of various graphs from those of the complete graph. We also investigate the symmetry of facet inducing inequalities of the cut polytope of the complete bipartite graph derived by triangular elimination.Comment: 19 pages, 1 figure; filled details of the proof of Theorem 4, made many other small change

Shared Randomness and Quantum Communication in the Multi-Party Model

We study shared randomness in the context of multi-party number-in-hand communication protocols in the simultaneous message passing model. We show that with three or more players, shared randomness exhibits new interesting properties that have no direct analogues in the two-party case. First, we demonstrate a hierarchy of modes of shared randomness, with the usual shared randomness where all parties access the same random string as the strongest form in the hierarchy. We show exponential separations between its levels, and some of our bounds may be of independent interest. For example, we show that the equality function can be solved by a protocol of constant length using the weakest form of shared randomness, which we call "XOR-shared randomness." Second, we show that quantum communication cannot replace shared randomness in the k-party case, where k >= 3 is any constant. We demonstrate a promise function GP_k that can be computed by a classical protocol of constant length when (the strongest form of) shared randomness is available, but any quantum protocol without shared randomness must send n^Omega(1) qubits to compute it. Moreover, the quantum complexity of GP_k remains n^Omega(1) even if the "second strongest" mode of shared randomness is available. While a somewhat similar separation was already known in the two-party case, in the multi-party case our statement is qualitatively stronger: * In the two-party case, only a relational communication problem with similar properties is known. * In the two-party case, the gap between the two complexities of a problem can be at most exponential, as it is known that 2^(O(c)) log n qubits can always replace shared randomness in any c-bit protocol. Our bounds imply that with quantum communication alone, in general, it is not possible to simulate efficiently even a three-bit three-party classical protocol that uses shared randomness.Comment: 14 pages; v2: improved presentation, corrected statement of Theorem 2.1, corrected typo