In horses undergoing volatile anaesthesia, is recovery quality superior with sevoflurane compared to isoflurane?

PICO question In horses undergoing volatile anaesthesia, is recovery quality superior with the use of sevoflurane compared to isoflurane during the maintenance phase?   Clinical bottom line The category of research question Treatment Number and type of study designs reviewed Seven papers were available for critical appraisal. Of the seven papers, six were prospective, randomised trials and four of these were of crossover design. Of the same seven papers, three were experimental and four were clinical. Strength of evidence Moderate Outcomes reported Five out of seven critically appraised articles found that there was no clinically significant improvement in recovery quality following volatile anaesthesia with sevoflurane compared to isoflurane. Two of the seven articles did find improvement in recovery quality following the use of sevoflurane over isoflurane, but both studies were of crossover design, one of these studies used non-blinded evaluators and the second study used both unblinded and blinded evaluators and a recovery quality scoring scale that did not show interobserver reliability. Conclusion In healthy horses presented for elective surgical and diagnostic imaging procedures in a clinical setting, there is no significant difference in recovery quality following the use of sevoflurane or isoflurane for the maintenance phase.   How to apply this evidence in practice The application of evidence into practice should take into account multiple factors, not limited to: individual clinical expertise, patient’s circumstances and owners’ values, country, location or clinic where you work, the individual case in front of you, the availability of therapies and resources. Knowledge Summaries are a resource to help reinforce or inform decision making. They do not override the responsibility or judgement of the practitioner to do what is best for the animal in their care

Integrated assessment of medicinal rhubarb by combination of delayed luminescence and HPLC fingerprint with emphasized on bioactivities based quality control

Plant science

Computed Tomography-Derived Occipital–Coccygeal Length and Ilium Wing Distance Correlates with Skin to Epidural and Intrathecal Depths in Dogs

The current clinical techniques for neuraxial needle placement in dogs are predominantly blind without prior knowledge of the depth required to reach the desired space. This study investigated the correlation and defined the relationship between easily obtainable external landmark variables in the dog; occipital–coccygeal length (OCL) and ilium wings distance (IWD), with the skin to epidural and intrathecal space distances using computed tomography (CT). The CT images of 86 dogs of different breeds were examined in this retrospective observational study. Images of dogs in sternal recumbency were optimized to the sagittal view. The distances between the skin and lumbosacral epidural space (LSE) and skin to sacrococcygeal space (SCE) were measured to the ligamentum flavum surrogate (LFS) line. The distance between the skin and the intrathecal space (ITS) was measured from the skin to the vertebral canal at the interlumbar (L5–L6) space. Measurements of the IWD and OCL were performed on dorsal and scout views, respectively. Linear regression equations and Pearson’s correlation coefficients were calculated between variables. Data were reported as mean (standard deviation). Significance was set as alpha < 0.05. After exclusion of four dogs, 82 CT scans were included. The depths were LSE 45 (15) mm, SCE 23 (10) mm, and ITS 50 (15) mm. There was a moderate correlation between OCL with LSE (=14.2 + OCL * 0.05 (r = 0.59, p < 0.0001)), and a strong correlation with ITS (=11.4 + OCL * 0.07 (r = 0.76, p < 0.0001)), while a very weak correlation was found with SCE (=14.0 + OCL * 0.02 (r = 0.27, p < 0.0584)). Similarly, with IWD, there was a moderate correlation with LSE (=10.8 + IWD * 0.56 (r = 0.61, p < 0.0001)), and strong correlation with ITS (=9.2 + IWD * 0.67 (r = 0.75, p < 0.0001)), while a weak correlation was found with SCE (=11.2 + IWD * 0.2 (r = 0.32, p < 0.0033)). Mathematical formulae derived from the multiple regression showed that the body condition score (BCS) improved the relationship between IWD and OCL and the LSE, SCE and ITS, while the addition of body weight was associated with multicollinearity. Further studies are required to determine the accuracy of the algorithms to demonstrate their ability for prediction in a clinical setting

Xiao Cheng Qi Decoction, an Ancient Chinese Herbal Mixture, Relieves Loperamide-Induced Slow-Transit Constipation in Mice: An Action Mediated by Gut Microbiota

Xiao Cheng Qi (XCQ) decoction, an ancient Chinese herbal mixture, has been used in treating slow-transit constipation (STC) for years. The underlying action mechanism in relieving the clinical symptoms is unclear. Several lines of evidence point to a strong link between constipation and gut microbiota. Short-chain fatty acids (SCFAs) and microbial metabolites have been shown to affect 5-HT synthesis by activating the GPR43 receptor localized on intestinal enterochromaffin cells, since 5-HT receptors are known to influence colonic peristalsis. The objective of this study was to evaluate the efficacy of XCQ in alleviating clinical symptoms in a mouse model of STC induced by loperamide. The application of loperamide leads to a decrease in intestinal transport and fecal water, which is used to establish the animal model of STC. In addition, the relationship between constipation and gut microbiota was determined. The herbal materials, composed of Rhei Radix et Rhizoma (Rhizomes of Rheum palmatum L., Polygonaceae) 55.2 g, Magnoliae Officinalis Cortex (Barks of Magnolia officinalis Rehd. et Wils, Magnoliaceae) 27.6 g, and Aurantii Fructus Immaturus (Fruitlet of Citrus aurantium L., Rutaceae) 36.0 g, were extracted with water to prepare the XCQ decoction. The constipated mice were induced with loperamide (10 mg/kg/day), and then treated with an oral dose of XCQ herbal extract (2.0, 4.0, and 8.0 g/kg/day) two times a day. Mosapride was administered as a positive drug. In loperamide-induced STC mice, the therapeutic parameters of XCQ-treated mice were determined, i.e., (i) symptoms of constipation, composition of gut microbiota, and amount of short-chain fatty acids in feces; (ii) plasma level of 5-HT; and (iii) expressions of the GPR43 and 5-HT4 receptor in colon. XCQ ameliorated the constipation symptoms of loperamide-induced STC mice. In gut microbiota, the treatment of XCQ in STC mice increased the relative abundances of Lactobacillus, Prevotellaceae_UCG_001, Prevotellaceae_NK3B31_group, Muribaculaceae, and Roseburia in feces and decreased the relative abundances of Desulfovibrio, Tuzzerella, and Lachnospiraceae_ NK4A136_group. The levels of SCFAs in stools from the STC group were significantly lower than those the control group, and were greatly elevated via treatment with XCQ. Compared with the STC group, XCQ increased the plasma level of 5-HT and the colonic expressions of the GPR43 and 5-HT4 receptor, significantly. The underlying mechanism of XCQ in anti-constipation could be related to the modulation of gut microbiota, the increase in SCFAs, the increase in plasma 5-HT, and the colonic expressions of the GPR43 and 5-HT4 receptor. Our results indicate that XCQ is a potent natural product that could be a therapeutic strategy for constipation

Jellyfish genomes reveal distinct homeobox gene clusters and conservation of small RNA processing

The phylum Cnidaria represents a close outgroup to Bilateria and includes familiar animals including sea anemones, corals, hydroids, and jellyfish. Here we report genome sequencing and assembly for true jellyfish Sanderia malayensis and Rhopilema esculentum. The homeobox gene clusters are characterised by interdigitation of Hox, NK, and Hox-like genes revealing an alternate pathway of ANTP class gene dispersal and an intact three gene ParaHox cluster. The mitochondrial genomes are linear but, unlike in Hydra, we do not detect nuclear copies, suggesting that linear plastid genomes are not necessarily prone to integration. Genes for sesquiterpenoid hormone production, typical for arthropods, are also now found in cnidarians. Somatic and germline cells both express piwi-interacting RNAs in jellyfish revealing a conserved cnidarian feature, and evidence for tissue-specific microRNA arm switching as found in Bilateria is detected. Jellyfish genomes reveal a mosaic of conserved and divergent genomic characters evolved from a shared ancestral genetic architecture