In vitro anti-HIV-1 properties of ethnobotanically selected South African plants used in the treatment of sexually transmitted diseases

Please read the abstract in the section of 00front of this documentThesis (PhD (Medical Plant Science))--University of Pretoria, 2007.Plant Scienceunrestricte

Antimicrobial Constituents of Artemisia afra Jacq. ex Willd. against Periodontal Pathogens

The phytochemical investigation of an ethanol extract of Artemisia afra led to the isolation of six known compounds, acacetin (1), 12α,4α-dihydroxybishopsolicepolide (2), scopoletin (3), α-amyrin (4), phytol (5), and a pentacyclic triterpenoid betulinic acid (6). The compounds were evaluated for antimicrobial activity against Gram positive (Actinomyces naeslundii, Actinomyces israelii, and Streptococcus mutans), Gram negative bacteria (Prevotella intermedia, Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans previously known as Actinobacillus actinomycetemcomitans), and Candida albicans. The crude extract of A. afra inhibited the growth of all tested microbial species at concentration range of 1.6 mg/mL to 25 mg/mL. The compounds 1–6 also showed activity range at 1.0 mg/mL to 0.25 mg/mL. Three best compounds (scopoletin, betulinic acid, and acacetin) which showed good antimicrobial activity were selected for further studies. Cytotoxicity of extract and compounds was determined using the XTT cell proliferation kit. The antioxidant activity of the extract and compounds was done using the DPPH scavenging method. The extract showed good antioxidant activity with an IC50 value of 22.2 μg/mL. Scopoletin had a strong transformation of the DPPH radical into its reduced form, with an IC50 value of 1.24 μg/mL which was significant to that of vitamin C (1.22 μg/mL). Acacetin and betulinic acid exhibited a decreased scavenging activity with the IC50 of 2.39 and 2.42 μg/mL, respectively. The extract and compounds showed moderate toxicity on McCoy fibroblast cell line and scopoletin was relatively nontoxic with an IC50 value of 132.5 μg/mL. Acacetin and betulinic acid also showed a smooth trend of non-toxic effects with IC50 values of 35.44 and 30.96 μg/mL. The obtained results in this study confirm the use of A. afra in the treatment of microbial infections

An ethnobotanical study of medicinal plants used in villages under Jongilanga Tribal Council, Mpumalanga, South Africa

Background: Medicinal plants remain an integral part of the lives of people in rural areas. The aim of this study was to document information about the medicinal plants used by Shangaan people in villages under Jongilanga tribal council, Bushbuckridge municipality, Mpumalanga Province, South Africa.Materials and Methods: An ethnobotanical survey of medicinal plants was conducted using a semi-structured questionnaire with 15 traditional healers as informants; one of them also served as a field guide during data collection. Results were analysed by using various quantitative indices of information consensus factor (ICF), use report (UR), frequency citation (FC) and relative frequency citation (RFC).Results and Methods: The study reported 86 medicinal plants used in villages for the treatment of various ailments, the majority (25 species) of which were used for urino-genital disorders. The Fabaceae family was the most represented family (17 species) of all the medicinal plants recorded in this study. The roots were the most frequently used plant part, accounting for 56% of the plants reported, and decoctions were often used in the preparation of herbal remedies. Respiratory diseases had the highest ICF value recorded among the 8 categories of ailments. The highest use report was reported for Combretum collinum (4), while the FC and RFC values (15) were highest in 12 plant species. The study revealed that medicinal plants are still widely used in rural areas and this documentation can serve as an ethno pharmacological basis for selecting plants with potential pharmaceutical properties.Keywords: Medicinal plants, Traditional healers, Jongilang

Anti-microbial, anti-Inflammatory and HIV-1 reverse transcriptase activity of selected South African plants used to treat sexually transmitted diseases

The purpose of the study was to investigate the bioactivity of extracts of selected plant species used to treat sexually transmitted diseases (STD’s) in southern Africa. As the emergence of drug resistance pathogens in STD’s treatment and potential side effects of synthetic drugs demands the discovery of newer and safer drugs, the exploration of newer anti-microbial substances from natural sources may serve as promising alternatives. Ethanol extracts of twelve medicinal plants used traditionally to treat sexually transmitted diseases and 3 flavonoids (F1, F2 and F3) isolated from Elaeodendron transvaalense were evaluated for their antimicrobial properties against one fungus and three bacteria. To determined anti-inflammatory activities of the extracts and compounds, the inhibitory effect was measured on the pro-inflammatory enzyme, 15-lipoxygenase (15-LOX). The extracts and compounds were also investigated for their anti-HIV activity against recombinant HIV-1 enzyme using non-radioactive HIV-RT colorimetric assay. Acacia karoo and Rhoicissus tridentata extracts indicated good anti-microbial activity with MIC values ranging between 0.4 and 3.1 mg/mL. Extracts of Jasminum fluminense, Solanum tomentosum, F2 and F3 had good anti-inflammatory activity with IC50 less than positive control quercetin (IC50 = 48.86 μg/mL). Acacia karoo and F3 exhibited moderate HIV RT inhibition activity of 66.8 and 63.7% respectively. Rhoicissus tridentata and Terminalia sericea had the best RT inhibition activity (75.7 and 100%) compared to that of the positive control doxorubicin (96.5%) at 100 μg/mL. The observed activities may lead to new multi-target drug against sexually transmitted diseases.The National research Foundation (Grant: 99022)http://www.ijppr.comam2017Plant Production and Soil Scienc

Antimicrobial, antioxidant and cytotoxicity studies of medicinal plants used in the treatment of sexually transmitted diseases

CONTEXT : The World Health Organization reported that more than 1 million sexually transmitted infections (STIs) are acquired daily, with an estimated 357 million new infections caused by either chlamydia, gonorrhoea, syphilis or trichomoniasis. Aim: The present study was aimed to evaluate antimicrobial, antioxidant and cytotoxicity activities of ethanol extracts of ten ethnobotanical selected plant species used to treat STD’s and related symptoms. Methods: The determination of antimicrobial susceptibility of plant extracts was done using the broth micro-dilution assay against five microorganisms. The free radical scavenging activity was determined by using DPPH (2, 2-diphenyly-1-picrylhydrazyl). Cytotoxicity activity of the plant extracts was done on Vero African monkey cells lines with 2, 3-bis [2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide (XTT) reagents. Results: Our results suggest that extracts of Acacia karroo and Rhoicissus tridentata subsp. cuneifolia are potential candidates with a good antimicrobial, antioxidant and low cytotoxicity activities. This results may support the anecdotal claims for the use of the selected plant species to treat venereal diseases.The National Research Foundation (NRF Grant no: 99022)http://www.ijppr.com/am2017Plant Production and Soil Scienc

ANTIMICROBIAL AND ANTI-INFLAMMATORY ACTIVITIES OF PLEUROSTYLIA CAPENSIS TURCZ (LOES) (CELASTRACEAE).

Background: Pleurostylia capensis is a large tree that can reach the maximum height of 20 m long, and it have been traditionally used as cosmetic, for steam bath, ritual body wash, and as a purgative to treat symptoms of witchcraft. Using ethanol, chloroform, dichloromethane (DCM), ethyl acetate (EA), and water extracts, leaves, bark and roots of Pleurostylia capensis were investigated scientifically for their effectiveness in antimicrobial, antioxidant and anti-inflammatory activities using standard methods Materials and Methods: The extracts were evaluated for antimicrobial activity against Gram positive (Staphylococcus aureus, Bacillus cereus, and Mycobacterium smegmatis), Gram negative (Escherichia coli, Klebsiella pneumonia, Klebsiella oxytoca, Streptococcus pyogenes, Pseudomonas aeruginosa and Salmonella typhimurium), and Candida albicans. The antioxidant activity was investigated using 2, 2-diphenlyl-1-picrylhadrazyl (DPPH), free radical scavenging assay. The anti-inflammatory activity of P. capensis extracts was evaluated against both cyclooxygenase enzymes (COX 1 and 2). Results: The ethyl acetate extracts of P. capensis showed a strong antimicrobial activity against B. cereus, K. pneumonia, S. pyogenes, and M. smegmatis with MIC value of 0.39 and 0.78 mg/ml. While the ethanol bark extract was most active against M. smegmatis with MIC value of 0.78 mg/ml; the least potent activity was observed with dichloromethane, chloroform and water extracts, with an MIC value ranging from 1.56 mg/ml to 50.0 mg/ml. The plant extracts proved to be good antioxidant agent, whereas extracts of ethanol were the most active, with IC50 ranging from 1.00 to 1.74 μg/ml, which is lower, and in close range to Vitamin C (1.40 μg/ml). Conclusions: Its moderation to potent inhibitory activity was observed in all extracts. Ethanol and dichloromethane extracts were among the most potent when compared to water and petroleum ether extracts. The water extracts showed to be nontoxic on the Hek cell line with an IC50 value of 204.0, and 207.3 μg/ml (roots and bark) respectively. The dichloromethane, ethyl acetate, chloroform and ethanol extracts showed to be toxic on the Hek cell, with IC50 range from 5.94 to 42.91μg/ml. The results obtained indicate the effectiveness of these plants

An ethnobotanical study of medicinal plants used in villages under Jongilanga tribal council, Mpumalanga, South Africa

BACKGROUND : Medicinal plants remain an integral part of the lives of people in rural areas. The aim of this study was to document information about the medicinal plants used by Shangaan people in villages under Jongilanga tribal council, Bushbuckridge municipality, Mpumalanga Province, South Africa. MATERIALS AND METHODS : An ethnobotanical survey of medicinal plants was conducted using a semi-structured questionnaire with 15 traditional healers as informants; one of them also served as a field guide during data collection. Results were analysed by using various quantitative indices of information consensus factor (ICF), use report (UR), frequency citation (FC) and relative frequency citation (RFC). RESULTS AND METHODS : The study reported 86 medicinal plants used in villages for the treatment of various ailments, the majority (25 species) of which were used for urino-genital disorders. The Fabaceae family was the most represented family (17 species) of all the medicinal plants recorded in this study. The roots were the most frequently used plant part, accounting for 56% of the plants reported, and decoctions were often used in the preparation of herbal remedies. Respiratory diseases had the highest ICF value recorded among the 8 categories of ailments. The highest use report was reported for Combretum collinum (4), while the FC and RFC values (15) were highest in 12 plant species. The study revealed that medicinal plants are still widely used in rural areas and this documentation can serve as an ethno pharmacological basis for selecting plants with potential pharmaceutical properties.Mr. H. Chc M Christian Courtin, represented by Stefan Winterboer of Guides and Trackers.http://journals.sfu.ca/africanem/index.php/ajtcamam2017Plant Production and Soil Scienc

Quorum sensing modulation and inhibition in biofilm forming foot ulcer pathogens by selected medicinal plants

The crisis of antibiotic resistance necessitates the search of phytochemicals as potential antibacterial, anti-quorum sensing and antibiofilm forming agents. For the present study, fifteen (15) selected medicinal plants were evaluated to inhibit the biological activities of multi-drug resistant (MDR) pathogenic bacteria (Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis) associated with diabetic foot ulcer. Antibacterial activities revealed noteworthy minimum inhibitory concentration (MIC) values ≤1 mg/mL for thirteen (13) out of the sixty (60) plant extracts screened. The potent extracts included Euclea natalensis ethyl acetate (0.25 mg/mL), Aloe ferox methanol (0.5 mg/ml) and Warburgia salutaris aqueous (0.5 mg/mL) extracts. Chemical profiling of the active extracts using gas chromatography-mass spectrometry (GC-MS) identified neophytadiene, guanosine, squalene, cis megastigma-5,8-diene-4-one and sorbitol as prevalent compounds among the active extracts. Anti-quorum sensing activities of E. natalensis (ethyl acetate), A. ferox (methanol) and W. salutaris (aqueous) extracts ranged from 4.81 - 58.34% with E. natalensis (ethyl-acetate) showing the highest activity. Molecular docking against CviR protein showed selected compounds having high docking scores with sorbitol showing the highest score of -7.04 kcal/mol. Warburgia salutaris aqueous extract exhibited the highest biofilm inhibition (73%) against E. coli. Euclea natalensis, Aloe ferox and Warburgia salutaris compounds act as antagonist of N-acyl homoserine lactone (AHL) signaling, thus may serve as candidates in antipathogenic and antibiofilm phytomedicine development for MDR foot ulcer bacterial pathogens.The South African National Research Foundation (NRF) Thuthuka Grant and the South African Medical Research Council-Self Initiated Research (SAMRC-SIR).https://www.cell.com/heliyon/homeBiochemistryGeneticsMicrobiology and Plant PathologyPlant Production and Soil Scienc

AN ETHNOBOTANICAL STUDY OF MEDICINAL PLANTS USED IN VILLAGES UNDER JONGILANGA TRIBAL COUNCIL, MPUMALANGA, SOUTH AFRICA

Background: Medicinal plants remain an integral part of the lives of people in rural areas. The aim of this study was to document information about the medicinal plants used by Shangaan people in villages under Jongilanga tribal council, Bushbuckridge municipality, Mpumalanga Province, South Africa. Materials and Methods: An ethnobotanical survey of medicinal plants was conducted using a semi-structured questionnaire with 15 traditional healers as informants; one of them also served as a field guide during data collection. Results were analysed by using various quantitative indices of information consensus factor (ICF), use report (UR), frequency citation (FC) and relative frequency citation (RFC). Results and Methods: The study reported 86 medicinal plants used in villages for the treatment of various ailments, the majority (25 species) of which were used for urino-genital disorders. The Fabaceae family was the most represented family (17 species) of all the medicinal plants recorded in this study. The roots were the most frequently used plant part, accounting for 56% of the plants reported, and decoctions were often used in the preparation of herbal remedies. Respiratory diseases had the highest ICF value recorded among the 8 categories of ailments. The highest use report was reported for Combretum collinum (4), while the FC and RFC values (15) were highest in 12 plant species. The study revealed that medicinal plants are still widely used in rural areas and this documentation can serve as an ethno pharmacological basis for selecting plants with potential pharmaceutical properties