Cosa Sekelwa

Rakoma Jostina

Tshikalange Emmanuel

University of Szeged

Trends in Natural Product Research – PSE Young Scientists’ Meeting Budapest, June 19th-21st, 2019 91  PO-9 doi: 10.14232/tnpr.2019.po9  Calpurnia aurea ethanol extract reduces Pseudomonas aeruginosa quorum sensing- dependent virulence factors  Jostina Rakoma1, Emmanuel Tshikalange2 and Sekelwa Cosa1, *  1 Department of Biochemistry, Genetics and Microbiology, University of Pretoria, Private Bag X20, Hatfield 0028, South Africa. 2 Department of Plant Sciences, University of Pretoria, Private Bag X20, Hatfield 0028, South Africa.   *E-mail: sekelwa.cosa@up.ac.za   Pseudomonas aeruginosa is the causative agent of several life-threatening human infections like urinary tract infections, lung infections and skin infections among many. P. aeruginosa, like many other pathogens exhibits quorum sensing controlled virulence factors such as biofilm during disease progression, complicating the treatment with conventional antibiotics. Thus, impeding with the pathogen’s QS circuit appears as promising new strategy to overcome pseudomonad infections. In the present study, Calpurnia aurea ethanol extract, along with other extracts (acetone, ethyl acetate, water) of Leonotis ocymifolia and Moringa oleifera were evaluated for anti-quorum sensing/ anti-virulence potential against Pseudomonas aeruginosa. Calpurnia aurea extracts demonstrated effective antimicrobial activities with MIC value between 1.5-3.5 mg/mL. Calpurnia aurea ethanol extracts demonstrated effective anti-QS and anti-virulence (biofilm formation, motility, pyocyanin and pyoverdine production). The quantitative violacein inhibition of Calpurnia aurea ethanol extracts showed ≥ 51 % at 1 mg/mL.  P. aeruginosa was significantly inhibited by ≥ 50% with same plant extract at 1 mg/mL, whereas observation of dead/live cells with confocal laser microscopy showed a significant reduction of biofilm formation. While extracts altered swimming motility, swarming motility was not significantly altered. Ethanolic Calpurnia aurea extracts reduced pseudomonad virulence, albeit in a strain- and extract-specific manner.  Calpurnia aurea extracts may be suitable for identification of lead compounds with QS inhibitory potential for control of P. aeruginosa infections.  Acknowledgements Authors would like to acknowledge the National Research Foundation of South Africa (NRF Thuthuka programme), and the University of Pretoria.   

Calpurnia aurea ethanol extract reduces Pseudomonas aeruginosa quorum sensing- dependent virulence factors : [abstract]

http://acta.bibl.u-szeged.hu/62020/1/ysm_2019_091.pdf

Calpurnia aurea ethanol extract reduces Pseudomonas aeruginosa quorum sensing- dependent virulence factors

Abstract

Similar works

Full text

Available Versions

University of Szeged