Zero % long term biliary stricture in microscopic reconstruction (MBR) of Hepatico-Jejunal Biliary Roux en Y choice of biliary drainage of adult liver transplant

BackgroundRoutine use of (MBR) by Roux en Y in adult Orthotopic Liver Transplantation (OLT) has not been elucidated. The usual choice of enteric drainage got expected morbidities of biliary enteric anastomosis. Patients of liver transplant clinical differences are compared. Choices, current status, efficacy, application, short and long term outcome of biliary reconstruction by (MBR) roux en Y anastomosis in adult liver transplant was compared to Conventional roux en y.AimThe primary aim of the study is to clarify the influence to the diseased liver recipient duct to the future graft biliary drainage.MethodsStudy of consecutive liver transplant patients was retrieved. Total Number of 1234 OLT, By the End of July 2014. Group A 16 patients of Conventional Period up to 22 March, 2006. Group B 50 patients of (MBR) up to 31 JULY 2014.ResultsIn group A 8/16 got short and long term complication. However, in group B only 4/50 got short term problems, with no death.ConclusionIn Spite of the drawback of adult OLT roux en Y hepatico-jejunal anastomosis including anatomical challenges and graft position, we developed graft survival in adults liver transplant with widened application of (MBR).There is accessible and durable intact biliary drainage choice by (MBR) hepatica enteric anastomosis for adults OLT patients that can be encouraged and advised by microscopic surgery

Deactivation of TBP contributes to SCA17 pathogenesis

Spinocerebellar ataxia type 17 (SCA17) is an autosomal dominant cerebellar ataxia caused by the expansion of polyglutamine (polyQ) within the TATA box-binding protein (TBP). Previous studies have shown that polyQexpanded TBP forms neurotoxic aggregates and alters downstream genes. However, how expanded polyQ tracts affect the function of TBP and the link between dysfunctional TBP and SCA17 is not clearly understood. In this study, we generated novel Drosophila models for SCA17 that recapitulate pathological features such as aggregate formation, mobility defects and premature death. In addition to forming neurotoxic aggregates, we determined that polyQ-expanded TBP reduces its own intrinsic DNA-binding and transcription abilities. Dysfunctional TBP also disrupts normal TBP function. Furthermore, heterozygous dTbp amorph mutant flies exhibited SCA17-like phenotypes and flies expressing polyQ-expanded TBP exhibited enhanced retinal degeneration, suggesting that loss of TBP function may contribute to SCA17 pathogenesis. We further determined that the downregulation of TBP activity enhances retinal degeneration in SCA3 and Huntington's disease fly models, indicating that the deactivation of TBP is likely to play a common role in polyQ-induced neurodegeneration

Bulbophyllum albociliatum (Liu & Su) Nakejima var. weiminianum and Flickingeria shihfuana, Two New Native Orchids from Taiwan

In this report, two newly discovered native orchids, Bulbophyllum albociliatum (Liu & Su) Nakejima var. weiminianum and Flickingeria shihfuana, are presented

Cloning and Expression of a Schwanniomyces occidentalis α-Amylase Gene in Saccharomyces cerevisiae

An α-amylase gene (AMY) was cloned from Schwanniomyces occidentalis CCRC 21164 into Saccharomyces cerevisiae AH22 by inserting Sau3AI-generated DNA fragments into the BamHI site of YEp16. The 5-kilobase insert was shown to direct the synthesis of α-amylase. After subclones containing various lengths of restricted fragments were screened, a 3.4-kilobase fragment of the donor strain DNA was found to be sufficient for α-amylase synthesis. The concentration of α-amylase in culture broth produced by the S. cerevisiae transformants was about 1.5 times higher than that of the gene donor strain. The secreted α-amylase was shown to be indistinguishable from that of Schwanniomyces occidentalis on the basis of molecular weight and enzyme properties

Ultrastructural Study on the Recalcitrant Seeds of Machilus thunbergii Sieb. & Zucc.

The recalcitrant (desiccation sensitive) seeds of Machilus thunbergii were histochemically and ultrastructurally investigated by using light and electron microscopy. The embryo of mature seed has 4-6 subopposite primary leaves in the plumule. We found two types of idioblastic secretory cells (oil cells and mucilage cells) in the cotyledons, but observed only the oil cells in the embryonic axes. Cells in shoot and radicle meristem of the fresh mature embryo have a large nucleus and small vacuoles. The cytoplasm is dense with organelles, and the plastids contain some starch grains and few oil droplets. Under 75% relative humidity storage condition, the desiccation processes set off the subcellular damages (vacuolation, withdrawal of plasmalemma) of the mature seeds. Such processes intensified with time and ultimately resulted in the loss of viability

Newly Discovered Native Orchids of Taiwan

In this report, four newly discovered native orchids: Bulbophyllum fimbriperianthium, Eulophia pulchra var. actinomorpha, Saccolabiopsis wulaokenensis, and Tropidia nanhuae, including a new record of the genus, Saccolabiopsis, are presented