23,672 research outputs found
Recommended from our members
Global Optimization Of Quasi-Monoenergetic Electron Beams From Laser Wakefield Accelerators
We globally optimize a terawatt-laser-driven wakefield accelerator by systematically varying laser and target parameters to achieve 100 MeV electrons, 10% energy spread, 100 pC charge, 4 mrad divergence and 10 mrad pointing fluctuation with similar to 100% reproducibility, thereby meeting conditions for producing similar to 10(6) 200 keV X-ray photons/pulse by inverse Compton scatter.Physic
TagF-mediated repression of bacterial type VI secretion systems involves a direct interaction with the cytoplasmic protein Fha
The bacterial type VI secretion system (T6SS) delivers effectors into eukaryotic host cells or toxins into bacterial competitor for survival and fitness. The T6SS is positively regulated by the threonine phosphorylation pathway (TPP) and negatively by the T6SS-accessory protein TagF. Here, we studied the mechanisms underlying TagF-mediated T6SS repression in two distinct bacterial pathogens, Agrobacterium tumefaciens and Pseudomonas aeruginosa. We found that in A. tumefaciens, T6SS toxin secretion and T6SS-dependent antibacterial activity are suppressed by a two-domain chimeric protein consisting of TagF and PppA, a putative phosphatase. Remarkably, this TagF domain is sufficient to post-translationally repress the T6SS, and this inhibition is independent of TPP. This repression requires interaction with a cytoplasmic protein, Fha, critical for activating T6SS assembly. In P. aeruginosa, PppA and TagF are two distinct proteins that repress T6SS in a TPP-dependent and -independent pathways, respectively. P. aeruginosa TagF interacts with Fha1, suggesting that formation of this complex represents a conserved TagF-mediated regulatory mechanism. Using TagF variants with substitutions of conserved amino acid residues at predicted protein-protein interaction interfaces, we uncovered evidence that the TagF-Fha interaction is critical for TagF-mediated T6SS repression in both bacteria. TagF inhibits T6SS without affecting T6SS protein abundance in A. tumefaciens, but TagF overexpression reduces the protein levels of all analyzed T6SS components in P. aeruginosa. Our results indicate that TagF interacts with Fha, which in turn could impact different stages of T6SS assembly in different bacteria, possibly reflecting an evolutionary divergence in T6SS control
Life table analysis of Diaphorina citri (Hemiptera: Psyllidae) infesting sweet orange (Citrus sinensis) in São Paulo
An ecological life table for eggs and nymphs of Diaphorina citri Kuwayama (Hemiptera:
Psyllidae) was constructed with data obtained from orange orchards (Citrus sinensis Osbeck) in 2 regions of the State of São Paulo, over 4 generations in the period from XI-2006
to V-2007, comprising spring, summer, and fall seasons. Young growing shoots with D. citri
eggs present were identifed, and live individuals were counted until adult emergence. No
predatory arthropods were observed in association with D. citri eggs and nymphs during
the study. The mean parasitism of fourth- and ffth-instar nymphs by Tamarixia radiata
Waterston (Hymenoptera: Eulophidae) was 2.3%. The durations of the egg–adult period
were similar among the 4 generations, ranging from 18.0 to 24.7 d (at mean temperatures
ranging from 21.6 to 26.0 °C) and followed the temperature requirement models obtained
in the laboratory for D. citri. However, survival from the egg to the adult stage for the same
period varied considerably from 1.7 to 21.4%; the highest mortalities were observed in the
egg and small nymphal (frst- to thirdinstar) stages, which were considered to be key phases
for population growth of the pest.Uma tabela de vida ecológica foi construÃda para ovos e ninfas de Diaphorina citri Kuwayama (Hemiptera: Psyllidae) com dados obtidos em pomares de laranja (Citrus sinensis Osbeck) em 2 regiões do estado de São Paulo, com 4 gerações, no perÃodo de novembro de 2006
a maio de 2007, compreendendo as estações de primavera, verão e outono. Ramos jovens
em crescimento com a presença de ovos de D. citri foram identificados e os indivÃduos vivos
foram contados até a emergência dos adultos. Nenhum predador foi observado associado a
ovos e ninfas de D. citri durante o estudo. A taxa média de parasitismo de ninfas de quarto
e quinto Ãnstares por Tamarixia radiata Waterson (Hymenoptera: Eulophidae) foi de 2.3%.
A duração do perÃodo de ovo a adulto foi semelhante entre as quatro gerações, variando de
18.0 a 24.7 dias (com temperaturas médias de 21.6 a 26.0 °C) e seguiram os modelos de
exigencias térmicas obtidas em laboratório para D. citri. Todavia, a sobrevivencia de ovo
até o estágio adulto variou consideravelmente para o mesmo perÃodo, de 1.7 a 21.4%, sendo
que as maiores mortalidades foram observadas nos estágios de ovos e ninfas pequenas (de
primeiro a terceiro Ãnstares), as quais foram consideradas fases chaves para o crescimento
populacional desta praga.info:eu-repo/semantics/publishedVersio
Optical Spectroscopic Survey of High-latitude WISE-selected Sources
We report on the results of an optical spectroscopic survey at high Galactic latitude (|b| ≥ 30°) of a sample of WISE-selected targets, grouped by WISE W1 (λ_eff = 3.4 μm) flux, which we use to characterize the sources WISE detected. We observed 762 targets in 10 disjoint fields centered on ultraluminous infrared galaxy candidates using DEIMOS on Keck II. We find 0.30 ± 0.02 galaxies arcmin–2 with a median redshift of z = 0.33 ± 0.01 for the sample with W1 ≥ 120 μJy. The foreground stellar densities in our survey range from 0.23 ± 0.07 arcmin–2 to 1.1 ± 0.1 arcmin–2 for the same sample. We obtained spectra that produced science grade redshifts for ≥90% of our targets for sources with W1 flux ≥120 μJy that also had an i-band flux gsim 18 μJy. We used this for targeting very preliminary data reductions available to the team in 2010 August. Our results therefore present a conservative estimate of what is possible to achieve using WISE's Preliminary Data Release for the study of field galaxies
Phase Structure of Kerr-AdS Black Hole
We study the critical phenomena of Kerr-AdS black hole. Phase structures are
observed at different temperatures, , and with various
features. We discuss the thermal stability considering the isothermal
compressibility and how phase transitions related to each other. The asymptotic
value of the angular momentum also has an implication on separating stable and
unstable part. Near critical temperature , the order parameter is
determined to calculate the critical exponents. All the critical exponents
(,,,)=(0,1/2,1,3) are identical to that of mean
field systems. We plot the phase diagram near this critical point, and discuss
the scaling symmetry of the free energy.Comment: 21 pages, 6 figures, contents revise
Deformation of anisotropic Fermi surfaces due to electron-electron interactions
We analyze the deformations of the Fermi surface induced by electron-electron
interactions in anisotropic two dimensional systems. We use perturbation theory
to treat, on the same footing, the regular and singular regions of the Fermi
surface. It is shown that, even for weak local coupling, the self-energy
presents a nontrivial behavior showing momentum dependence and interplay with
the Fermi surface shape. Our scheme gives simple analytical expressions based
on local features of the Fermi surface.Comment: 7 pages, 3 figure
Spontaneous Crystallization of Skyrmions and Fractional Vortices in the Fast-rotating and Rapidly-quenched Spin-1 Bose-Einstein Condensates
We investigate the spontaneous generation of crystallized topological defects
via the combining effects of fast rotation and rapid thermal quench on the
spin-1 Bose-Einstein condensates. By solving the stochastic projected
Gross-Pitaevskii equation, we show that, when the system reaches equilibrium, a
hexagonal lattice of skyrmions, and a square lattice of half-quantized vortices
can be formed in a ferromagnetic and antiferromagnetic spinor BEC, respetively,
which can be imaged by using the polarization-dependent phase-contrast method
Quantifying effective slip length over micropatterned hydrophobic surfaces
We employ micro-particle image velocimetry (-PIV) to investigate laminar
micro-flows in hydrophobic microstructured channels, in particular the slip
length. These microchannels consist of longitudinal micro-grooves, which can
trap air and prompt a shear-free boundary condition and thus slippage
enhancement. Our measurements reveal an increase of the slip length when the
width of the micro-grooves is enlarged. The result of the slip length is
smaller than the analytical prediction by Philip et al. [1] for an infinitely
large and textured channel comprised of alternating shear-free and no-slip
boundary conditions. The smaller slip length (as compared to the prediction)
can be attributed to the confinement of the microchannel and the bending of the
meniscus (liquid-gas interface). Our experimental studies suggest that the
curvature of the meniscus plays an important role in microflows over
hydrophobic micro-ridges.Comment: 8 page
Interstitial gas and density-segregation in vertically-vibrated granular media
We report experimental studies of the effect of interstitial gas on
mass-density-segregation in a vertically-vibrated mixture of equal-sized bronze
and glass spheres. Sufficiently strong vibration in the presence of
interstitial gas induces vertical segregation into sharply separated bronze and
glass layers. We find that the segregated steady state (i.e., bronze or glass
layer on top) is a sensitive function of gas pressure and viscosity, as well as
vibration frequency and amplitude. In particular, we identify distinct regimes
of behavior that characterize the change from bronze-on-top to glass-on-top
steady-state.Comment: 4 pages, 5 figures, submitted to PRL; accepted in PRE as rapid
communication, with revised text and reference
- …