4 research outputs found
Temporal dynamics of hot desert microbial communities reveal structural and functional responses to water input
8 páginas, 4 figuras. -- The first publication is available at https://www.nature.comThe temporal dynamics of desert soil microbial communities are poorly understood. Given the
implications for ecosystem functioning under a global change scenario, a better understanding of
desert microbial community stability is crucial. Here, we sampled soils in the central Namib Desert
on sixteen different occasions over a one-year period. Using Illumina-based amplicon sequencing of
the 16S rRNA gene, we found that α-diversity (richness) was more variable at a given sampling date
(spatial variability) than over the course of one year (temporal variability). Community composition
remained essentially unchanged across the first 10 months, indicating that spatial sampling might be
more important than temporal sampling when assessing β-diversity patterns in desert soils. However,
a major shift in microbial community composition was found following a single precipitation event. This
shift in composition was associated with a rapid increase in CO2 respiration and productivity, supporting
the view that desert soil microbial communities respond rapidly to re-wetting and that this response
may be the result of both taxon-specific selection and changes in the availability or accessibility of
organic substrates. Recovery to quasi pre-disturbance community composition was achieved within one
month after rainfall.We gratefully acknowledge financial support from the National Research Foundation of South Africa (grant no.81779 and TTK2008052000003), the Research Council of Norway (grant No. 180352) and the University of the Western Cape. Partial support was also provided under the Laboratory Directed Research and Development Program at PNNL, a multiprogram national laboratory operated by Battelle for the U.S. Department of Energy under contract DE-AC05-76RL01830.Peer reviewe
Induction of Sucrose Utilization Genes from Bifidobacterium lactis by Sucrose and Raffinose
The probiotic organism Bifidobacterium lactis was isolated from a yoghurt starter culture with the aim of analyzing its use of carbohydrates for the development of prebiotics. A sucrose utilization gene cluster of B. lactis was identified by complementation of a gene library in Escherichia coli. Three genes, encoding a sucrose phosphorylase (ScrP), a GalR-LacI-type transcriptional regulator (ScrR), and a sucrose transporter (ScrT), were identified by sequence analysis. The scrP gene was expressed constitutively from its own promoter in E. coli grown in complete medium, and the strain hydrolyzed sucrose in a reaction that was dependent on the presence of phosphates. Primer extension experiments with scrP performed by using RNA isolated from B. lactis identified the transcriptional start site 102 bp upstream of the ATG start codon, immediately adjacent to a palindromic sequence resembling a regulator binding site. In B. lactis, total sucrase activity was induced by the presence of sucrose, raffinose, or oligofructose in the culture medium and was repressed by glucose. RNA analysis of the scrP, scrR, and scrT genes in B. lactis indicated that expression of these genes was influenced by transcriptional regulation and that all three genes were similarly induced by sucrose and raffinose and repressed by glucose. Analysis of the sucrase activities of deletion constructs in heterologous E. coli indicated that ScrR functions as a positive regulator