Progress on indium and barium single ion optical frequency standards

We report progress on 115In+ and 137Ba+ single ion optical frequency standards using all solid-state sources. Both are free from quadrupole field shifts and together enable a search for drift in fundamental constants.Comment: 2 pages, 1 figure, submitted to IEEE/LEOS Summer 2005 Topicals conference proceeding

Stellar abundances and molecular hydrogen in high-redshift galaxies -the far-ultraviolet view

FUSE spectra of star-forming regions in nearby galaxies are compared to composite spectra of Lyman-break galaxies (LBGs), binned by strength of Lyman alpha emission and by mid-UV luminosity. Several far-UV spectral features, including lines dominated by stellar wind and by photospheric components, are very sensitive to stellar abundances. Their measurement in Lyman-break galaxies is compromised by the strong interstellar absorption features, allowing in some cases only upper limits. The derived C and N abundances in the LBGs are no higher than half solar (scaled to oxygen abundance for comparison with emission-line analyses), independent of the strength of Lyman alpha emission. P V absorption indicates abundances as low as 0.1 solar, with an upper limit near 0.4 solar in the reddest and weakest-emission galaxies. Unresolved interstellar absorption components would further lower the derived abundances. Trends of line strength, and derived abundances, are stronger with mid-UV luminosity than with Lyman-alpha strength. H2 absorption in the Lyman and Werner bands is very weak in the LBGs. Template H2 absorption spectra convolved to appropriate resolution show that strict upper limits N(H2)< 10^18 cm^-2 apply in all cases, with more stringent values appropriate for the stronger-emission composites and for mixes of H2 level populations like those on Milky Way sight lines. Since the UV-bright regions are likely to be widespread in these galaxies, these results rule out massive diffuse reservoirs of H2, and suggest that the dust/gas ratio is already fairly large at z~3.Comment: Astron J., in press (June 2006

An Overview of the Volatiles Investigating Polar Exploration Rover (VIPER) Mission

A critical goal to both science and exploration is to understand the form and location of lunar polar volatiles. The lateral and vertical distributions of these volatiles inform us of the processes that control the emplacement and retention of these volatiles, as well as helping to formulate in-situ resource utilization (ISRU) architectures. While significant progress has been made from orbital observations, measurements at a range of scales from centimeters to kilometers across the lunar surface are needed to generate adequate "volatile mineral models" for use in evaluating the resource potential of volatiles at the Moon. VIPER is a solar and battery powered rover mission designed to operate over multiple lunar days, traversing several kilometers as it continuously monitors for subsurface hydrogen and other surface volatiles. In specific thermal terrain types, including permanently shadowed terrain and locales that permit near-surface ice stability, subsurface samples will be examined for volatile content using a one-meter drill. This talk will provide an overview of the VIPER mission which is scheduled for flight to the Lunar South Pole in December 2022

\u3ci\u3eDrosophila\u3c/i\u3e SNAP-29 Is an Essential SNARE That Binds Multiple Proteins Involved in Membrane Traffic

Each membrane fusion event along the secretory and endocytic pathways requires a specific set of SNAREs to assemble into a 4-helical coiled-coil, the so-called trans-SNARE complex. Although most SNAREs contribute one helix to the trans-SNARE complex, members of the SNAP-25 family contribute two helixes. We report the characterization of the Drosophila homologue of SNAP-29 (dSNAP-29), which is expressed throughout development. Unlike the other SNAP-25 like proteins in fruit fly (i.e., dSNAP-25 and dSNAP-24), which form SDS-resistant SNARE complexes with their cognate SNAREs, dSNAP-29 does not participate in any SDS-resistant complexes, despite its interaction with dsyntaxin1 and dsyntaxin 16 in vitro. Immunofluorescence studies indicated that dSNAP-29 is distributed in various tissues, locating in small intracellular puncta and on the plasma membrane, where it associates with EH domain-containing proteins implicated in the endocytic pathway. Overexpression and RNAi studies suggested that dSNAP-29 mediates an essential process in Drosophila development

Order out of Randomness : Self-Organization Processes in Astrophysics

Self-organization is a property of dissipative nonlinear processes that are governed by an internal driver and a positive feedback mechanism, which creates regular geometric and/or temporal patterns and decreases the entropy, in contrast to random processes. Here we investigate for the first time a comprehensive number of 16 self-organization processes that operate in planetary physics, solar physics, stellar physics, galactic physics, and cosmology. Self-organizing systems create spontaneous {\sl order out of chaos}, during the evolution from an initially disordered system to an ordered stationary system, via quasi-periodic limit-cycle dynamics, harmonic mechanical resonances, or gyromagnetic resonances. The internal driver can be gravity, rotation, thermal pressure, or acceleration of nonthermal particles, while the positive feedback mechanism is often an instability, such as the magneto-rotational instability, the Rayleigh-B\'enard convection instability, turbulence, vortex attraction, magnetic reconnection, plasma condensation, or loss-cone instability. Physical models of astrophysical self-organization processes involve hydrodynamic, MHD, and N-body formulations of Lotka-Volterra equation systems.Comment: 61 pages, 38 Figure

A Platform-Independent Method for Detecting Errors in Metagenomic Sequencing Data: DRISEE

We provide a novel method, DRISEE (duplicate read inferred sequencing error estimation), to assess sequencing quality (alternatively referred to as “noise” or “error”) within and/or between sequencing samples. DRISEE provides positional error estimates that can be used to inform read trimming within a sample. It also provides global (whole sample) error estimates that can be used to identify samples with high or varying levels of sequencing error that may confound downstream analyses, particularly in the case of studies that utilize data from multiple sequencing samples. For shotgun metagenomic data, we believe that DRISEE provides estimates of sequencing error that are more accurate and less constrained by technical limitations than existing methods that rely on reference genomes or the use of scores (e.g. Phred). Here, DRISEE is applied to (non amplicon) data sets from both the 454 and Illumina platforms. The DRISEE error estimate is obtained by analyzing sets of artifactual duplicate reads (ADRs), a known by-product of both sequencing platforms. We present DRISEE as an open-source, platform-independent method to assess sequencing error in shotgun metagenomic data, and utilize it to discover previously uncharacterized error in de novo sequence data from the 454 and Illumina sequencing platforms.</p

Botulinum G neurotoxin cleaves VAMP/synaptobrevin at a single Ala-Ala peptide bond.

Similarly to other serotypes, botulinum neurotoxin serotype G (BoNT/G) contains the zinc binding motif of zinc endopeptidases. Highly purified preparations of BoNT/G show a zinc-dependent protease activity specific for VAMP/synaptobrevin, a membrane protein of synaptic vesicles. The two neuronal VAMP isoforms are cleaved with similar rates at one Ala-Ala peptide bond present in the same region, out of the several such peptide bonds present in their sequences. This site of cleavage is unique among the eight clostridial neurotoxins. VAMP proteolysis is displayed only after reduction of the single interchain disulfide bond present in the toxin, and it is inhibited by EDTA, o-phenanthroline and captopril