An extensive phenotypic characterization of the hTNFα transgenic mice

<p>Abstract</p> <p>Background</p> <p>Tumor necrosis factor alpha (TNFα) is implicated in a wide variety of pathological and physiological processes, including chronic inflammatory conditions, coronary artery disease, diabetes, obesity, and cachexia. Transgenic mice expressing human TNFα (hTNFα) have previously been described as a model for progressive rheumatoid arthritis. In this report, we describe extensive characterization of an hTNFα transgenic mouse line.</p> <p>Results</p> <p>In addition to arthritis, these hTNFα transgenic mice demonstrated major alterations in body composition, metabolic rate, leptin levels, response to a high-fat diet, bone mineral density and content, impaired fertility and male sexual function. Many phenotypes displayed an earlier onset and a higher degree of severity in males, pointing towards a significant degree of sexual dimorphism in response to deregulated expression of TNFα.</p> <p>Conclusion</p> <p>These results highlight the potential usefulness of this transgenic model as a resource for studying the progressive effects of constitutively expressed low levels of circulating TNFα, a condition mimicking that observed in a number of human pathological conditions.</p

Altered Emotional States in Knockout Mice Lacking 5-HT1A or 5-HT1B Receptors

International audienc

An extensive phenotypic characterization of the hTNFα transgenic mice-0

<p><b>Copyright information:</b></p><p>Taken from "An extensive phenotypic characterization of the hTNFα transgenic mice"</p><p>http://www.biomedcentral.com/1472-6793/7/13</p><p>BMC Physiology 2007;7():13-13.</p><p>Published online 10 Dec 2007</p><p>PMCID:PMC2222242.</p><p></p>pendently increased endogenous TNFα in WT and TG mice similarly. WT (open bars) and TG mice (closed bars) were injected with the indicated doses of LPS (μg/mouse) as shown on the X-axis. (A) Human and (B) mouse TNFα levels were measured using ELISA assays. Data are presented as means ± SEM, n = 5 for each genotype and dose combination. ND – non-detectable

An extensive phenotypic characterization of the hTNFα transgenic mice-2

<p><b>Copyright information:</b></p><p>Taken from "An extensive phenotypic characterization of the hTNFα transgenic mice"</p><p>http://www.biomedcentral.com/1472-6793/7/13</p><p>BMC Physiology 2007;7():13-13.</p><p>Published online 10 Dec 2007</p><p>PMCID:PMC2222242.</p><p></p>pendently increased endogenous TNFα in WT and TG mice similarly. WT (open bars) and TG mice (closed bars) were injected with the indicated doses of LPS (μg/mouse) as shown on the X-axis. (A) Human and (B) mouse TNFα levels were measured using ELISA assays. Data are presented as means ± SEM, n = 5 for each genotype and dose combination. ND – non-detectable

Author Correction: EMPReSS: standardized phenotype screens for functional annotation of the mouse genome

International audienceCorrection to: Nature Genetics, published online 1 November 2005.In the version of this article initially published, members of the Eumorphia Consortium appeared in the Supplementary Information but were not included in the main article. The full list of members appears below