How reliable are our beta-source calibrations?

International audienceThe calibration of any artificial-source attached to a luminescence reader is fundamental for the accuracy of luminescence dating results. Here, we present calibration results obtained for a-source attached to a single grain Risø reader in Bordeaux using a series of quartz of different origins. The quartz was irradiated with three different-irradiators. An unexpected variability of the apparent dose rates was observed and our results suggest that the-irradiation is the main reason for this variability. Further work is needed to clarify the underlying reasons

Apport des méthodes de la luminescence à la chronologie des techno-faciès du Middle Stone Age associés aux premiers hommes modernes du sud de l'Afrique

L'objectif de ce travail a été de préciser la chronologie de l'Howieson's Poort et du Still Bay, deux techno-faciès du Middle Stone Age (MSA) d'Afrique du Sud associés aux premiers Hommes anatomiquement modernes. Nous avons appliqué la méthode de datation par la luminescence à des pierres chauffées prélevées dans trois gisements. A Diepkloof Rock Shelter, nous proposons un âge préliminaire de 55-65ka pour le niveau Howieson's Poort. A Klasies River, nous avons obtenu 13 datations pour la séquence Howieson's Poort : l'âge moyen est 56+-3 ka et il est en accord avec les données TL/OSL et ESR. Ces nouveaux résultats vont cependant à l'encontre de certaines hypothèses formulées à partir de l'étude des paléoenvironnements. A Blombos Cave, un âge moyen de 74+-5 ka a été obtenu pour 5 échantillons des niveaux Still Bay. Cet âge qui est en accord avec d'autres données radiométriques (OSL et ESR), confirme l'antériorité de ce techno-faciès par rapport à celui de l'Howieson's Poort et situe, au moins à la fin du stade isotopique 5, l'ancienneté de certaines pratiques telles l'usage de symboles ou le travail de l'os

Luminescence age calculation through Bayesian convolution of equivalent dose and dose-rate distributions:The De_Dr model

In nature, each mineral grain (quartz or feldspar) receives a dose rate (Dr) specific to its environment. The dose-rate distribution, therefore, reflects the micro-dosimetric context of grains of similar size. If all the grains were well bleached at deposition, this distribution is assumed to correspond, within uncertainties, with the distribution of equivalent doses (De). The combination of the De and Dr distributions in the De_Dr model proposed here would then al- low calculation of the true depositional age. If grains whose De values are not representative of this age (hereafter called “outliers”) are present in the De distribution, this model al- lows them to be identified before the age is calculated, en- abling their exclusion. As the De_Dr approach relies only on the Dr distribution to describe the De distribution, the model avoids any assumption about the shape of the De distribu- tion, which can be difficult to justify. Herein, we outline the mathematical concepts of the De_Dr approach (more details are given in Galharret et al., 2021) and the exploitation of this Bayesian modelling based on an R code available in the R package “Luminescence”. We also present a series of tests using simulated Dr and De distributions with and without outliers and show that the De_Dr approach can be an alter- native to available models for interpreting De distributions.Using the world in ancient societies : processes and forms of appropriation of space in Long TimeCREDit - Chronological REference Datasets and Sites (CREDit) towards improved accuracy and precision in luminescence-based chronologie

Probable presence of an ubiquitous cryptic mitochondrial gene on the antisense strand of the cytochrome oxidase I gene

<p>Abstract</p> <p>Background</p> <p>Mitochondria mediate most of the energy production that occurs in the majority of eukaryotic organisms. These subcellular organelles contain a genome that differs from the nuclear genome and is referred to as mitochondrial DNA (mtDNA). Despite a disparity in gene content, all mtDNAs encode at least two components of the mitochondrial electron transport chain, including cytochrome <it>c </it>oxidase I (Cox1).</p> <p>Presentation of the hypothesis</p> <p>A positionally conserved ORF has been found on the complementary strand of the <it>cox1 </it>genes of both eukaryotic mitochondria (protist, plant, fungal and animal) and alpha-proteobacteria. This putative gene has been named <it>gau </it>for gene antisense ubiquitous in mtDNAs. The length of the deduced protein is approximately 100 amino acids. In vertebrates, several stop codons have been found in the mt <it>gau </it>region, and potentially functional <it>gau </it>regions have been found in nuclear genomes. However, a recent bioinformatics study showed that several hypothetical overlapping mt genes could be predicted, including <it>gau; </it>this involves the possible import of the cytosolic AGR tRNA into the mitochondria and/or the expression of mt antisense tRNAs with anticodons recognizing AGR codons according to an alternative genetic code that is induced by the presence of suppressor tRNAs. Despite an evolutionary distance of at least 1.5 to 2.0 billion years, the deduced Gau proteins share some conserved amino acid signatures and structure, which suggests a possible conserved function. Moreover, BLAST analysis identified rare, sense-oriented ESTs with poly(A) tails that include the entire <it>gau </it>region. Immunohistochemical analyses using an anti-Gau monoclonal antibody revealed strict co-localization of Gau proteins and a mitochondrial marker.</p> <p>Testing the hypothesis</p> <p>This hypothesis could be tested by purifying the <it>gau </it>gene product and determining its sequence. Cell biological experiments are needed to determine the physiological role of this protein.</p> <p>Implications of the hypothesis</p> <p>Studies of the <it>gau </it>ORF will shed light on the origin of novel genes and their functions in organelles and could also have medical implications for human diseases that are caused by mitochondrial dysfunction. Moreover, this strengthens evidence for mitochondrial genes coded according to an overlapping genetic code.</p

Increased oral nitric oxide in obstructive sleep apnoea

SummaryBackgroundHypoxia and snoring-related mechanical trauma contribute to airway inflammation in obstructive sleep apnoea (OSA). Increased exhaled nitric oxide (FENO), an airway inflammation marker, has been reported in OSA patients. We propose the measure of NO in the oral cavity (oNO) as marker of oropharyngeal inflammation in OSA.MethodsWe compared oNO and FENO of 39 OSA patients with those of 26 mild asthmatics (ASTHMA), 15 patients with chronic rhinitis or rhinosinusitis (CRS) and 24 healthy subjects. A special device was used for oNO measurement. Apnoea/hypopnoea index (AHI), oxygen desaturation index, mean and nadir SaO2 were calculated from the polysomnography.ResultsoNO was significantly increased in OSA (104.2 95%CI 80.2–135.5ppb) as compared to ASTHMA (71.9 95%CI 56.3–91.9ppb; p=0.015), CRS (54.4 95%CI 40.2–73.7ppb; p=0.009) and healthy subjects (63.6 95%CI 59–73ppb; p<0.001). oNO was directly related to AHI (r=0.466, p=0.003) and to minutes slept with SaO2 <90% (r=0.471, p=0.011) and it was inversely related to nadirSaO2 (r=−0.393, p=0.018). FENO was highest in asthmatics (40.3 95%CI 32.5–50.1ppb) and only slightly elevated in OSA (23.1 95%CI 19,8–28.3ppb) and CRS (22.8 95%CI 16.8–32.5ppb).ConclusionsThe finding that oral NO is increased in OSA and is related to upper airway obstructive episodes and to hypoxemia severity, strengthens the clinical and pathogenic role of oral inflammation in OSA

Technical note:On the reliability of laboratory beta-source calibration for luminescence dating

The dose rate of the 90Sr / 90Y beta source used in most luminescence readers is a laboratory key parameter. There is a well-established body of knowledge about parameters controlling accuracy and precision of the calibration value but some hard-to-explain inconsistencies still exist. Here, we have investigated the impact of grain size, aliquot size and irradiation geometry on the resulting calibration value through experiments and simulations. The resulting data indicate that the dose rate of an individual beta source results from the interplay of a number of parameters, most of which are well established by previous studies. Our study provides evidence for the impact of aliquot size on the absorbed dose in particular for grain sizes of 50–200 µm. For this grain-size fraction, the absorbed dose is enhanced by ∼ 10 %–20 % as aliquot size decreases due to the radial increase of dose rate towards the centre of the aliquot. The enhancement is most variable for 50–100 µm grains mounted as aliquots of &lt; 8 mm size. The enhancement is reversed when large grains are mounted as small aliquots due to the edge effect by which the dose induced by backscattered electrons is reduced. While the build-up of charge dictates the increase of absorbed dose with the increase of grain size, this principle becomes more variable with changing irradiation geometry. We conclude that future calibration samples should consist of subsamples composed of small, medium, large and very large quartz grains, each obtaining several gamma doses. The calibration value measured with small, medium and large aliquots is then obtained from the inverse slope of the fitted line, not from a single data point. In this way, all possible irradiation geometries of an individual beta source are covered, and the precision of the calibration is improved.</p