166 research outputs found

    First quarter bank results: good news, bad news

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    Banks and banking - West ; Banks and banking - California

    A Summary of Recent Damage-Initiation Experiments on KDP Crystals

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    ABSTRACT We summarize recent investigations of the density and morphology of bulk damage in KDP crystals as a function of pulse duration, temporal profile, wavelength, and energy fluence. As previously reported by Runkel et al. 1 , we also find that the size of bulk damage sites varies roughly linearly with pulse duration for pulses between 1 ns and 9 ns. However this trend no longer applies at pulse durations below 1 ns. Experiments measuring the damage density and size distribution as a function of wavelength confirm many previous works which indicated a strong dependence of damage density with wavelength. However, we also find that the size of damage sites is relatively insensitive to wavelength. Further we see damage due to Flat-In-Time (FIT) pulses has different pulse length and fluence dependence than Gaussian pulses. We demonstrate that a simple thermal diffusion model can account for observed differences in damage densities due to square and Gaussian temporally shaped pulses of equal fluence. Moreover, we show that the key laser parameter governing size of the bulk damage sites is the length of time the pulse remains above a specific intensity. The different dependences of damage density and damage site size on laser parameters suggest different absorption mechanisms early and late in the damaging pulse

    Community-Acquired Klebsiella pneumoniae Bacteremia: Global Differences in Clinical Patterns

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    We initiated a worldwide collaborative study, including 455 episodes of bacteremia, to elucidate the clinical patterns of Klebsiella pneumoniae. Historically, community-acquired pneumonia has been consistently associated with K. pneumoniae. Only four cases of community-acquired bacteremic K. pneumoniae pneumonia were seen in the 2-year study period in the United States, Argentina, Europe, or Australia; none were in alcoholics. In contrast, 53 cases of bacteremic K. pneumoniae pneumonia were observed in South Africa and Taiwan, where an association with alcoholism persisted (p=0.007). Twenty-five cases of a distinctive syndrome consisting of K. pneumoniae bacteremia in conjunction with community-acquired liver abscess, meningitis, or endophthalmitis were observed. A distinctive form of K. pneumoniae infection, often causing liver abscess, was identified, almost exclusively in Taiwan

    An improved method for undertaking limiting dilution assays for in vitro cloning of Plasmodium falciparum parasites

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    Abstract. Background: Obtaining single parasite clones is required for many techniques in malaria research. Cloning by limiting dilution using microscopy-based assessment for parasite growth is an arduous and labor-intensive process. An alternative method for the detection of parasite growth in limiting dilution assays is using a commercial ELISA histidine-rich protein II (HRP2) detection kit. Methods. Detection of parasite growth was undertaken using HRP2 ELISA and compared to thick film microscopy. An HRP2 protein standard was used to determine the detection threshold of the HRP2 ELISA assay, and a HRP2 release model was used to extrapolate the amount of parasite growth required for a positive result. Results: The HRP2 ELISA was more sensitive than microscopy for detecting parasite growth. The minimum level of HRP2 protein detection of the ELISA was 0.11ng/ml. Modeling of HRP2 release determined that 2,116 parasites are required to complete a full erythrocytic cycle to produce sufficient HRP2 to be detected by the ELISA. Under standard culture conditions this number of parasites is likely to be reached between 8 to 14 days of culture. Conclusions: This method provides an accurate and simple way for the detection of parasite growth in limiting dilution assays, reducing time and resources required in traditional methods. Furthermore the method uses spent culture media instead of the parasite-infected red blood cells, enabling culture to continue
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