Global Mapping Function (GMF): A new empirical mapping function based on numerical weather model data

Troposphere mapping functions are used in the analyses of Global Positioning System and Very Long Baseline Interferometry observations to map a priori zenith hydrostatic and wet delays to any elevation angle. Most analysts use the Niell Mapping Function (NMF) whose coefficients are determined from site coordinates and the day of year. Here we present the Global Mapping Function (GMF), based on data from the global ECMWF numerical weather model. The coefficients of the GMF were obtained from an expansion of the Vienna Mapping Function (VMF1) parameters into spherical harmonics on a global grid. Similar to NMF, the values of the coefficients require only the station coordinates and the day of year as input parameters. Compared to the 6-hourly values of the VMF1 a slight degradation in short-term precision occurs using the empirical GMF. However, the regional height biases and annual errors of NMF are significantly reduced with GMF

Laparoscopic reconstructive tubal surgery in a tertiary referral centre - a review of 177 cases

Objectives. To establish the pregnancy rate (PR) following endoscopic reconstructive tubal surgery in patients with tubal disease presenting to the Reproductive Medicine Unit at Groote Schuur Hospital between January 1994 and December 1997.Design. A prospective descriptive study utilising the Hulka classification system of tubal disease scored at the time of surgery and correlating the findings and procedures carried out with the pregnancy outcome.Setting and subjects. Patients with infertility referred from level 1 and 2 health care facilities in the Western Cape to the Reproductive Medicine Unit in the Department of Obstetrics and Gynaecology at Groote Schuur Hospital.Outcome measures. The main outcome measure was the PR following reconstructive surgery. Secondary outcome measures included the number of patients undergoing assisted reproductive techniques (ART) and the outcome of in vitro fertilisation and embryo transfer (IVFlET).Results. The results of 177 patients were analysed. The spontaneous PR for these patients was 13.6% per patient, with a live birth rate of 9%. The spontaneous extra-uterine pregnancy rate was 3.4% per patient and accounted for 25% of all spontaneous conceptions. Twenty-five patients (14% of the study population) underwent IVFlET resulting in a PR of 36% per patient and 33.3% per-embryo transfer.Conclusions. The poor PR following endoscopic tubal reconstruction is predominantly attributed to the severity of tubal damage in the study population. Patients undergoing IVFlET had a favourable PR - indicating that most of these couples are highly fertile but for the mechanical obstruction. The results of our study support ART as first-line therapy in the majority of patients with tubal factor infertility in the setting of this study

Pregnancy has a minimal impact on the acute transcriptional signature to vaccination.

Vaccination in pregnancy is an effective tool to protect both the mother and infant; vaccines against influenza, pertussis and tetanus are currently recommended. A number of vaccines with a specific indication for use in pregnancy are in development, with the specific aim of providing passive humoral immunity to the newborn child against pathogens responsible for morbidity and mortality in young infants. However, the current understanding about the immune response to vaccination in pregnancy is incomplete. We analysed the effect of pregnancy on early transcriptional responses to vaccination. This type of systems vaccinology approach identifies genes and pathways that are altered in response to vaccination and can be used to understand both the acute inflammation in response to the vaccine and to predict immunogenicity. Pregnant women and mice were immunised with Boostrix-IPV, a multivalent vaccine, which contains three pertussis antigens. Blood was collected from women before and after vaccination and RNA extracted for analysis by microarray. While there were baseline differences between pregnant and non-pregnant women, vaccination induced characteristic patterns of gene expression, with upregulation in interferon response and innate immunity gene modules, independent of pregnancy. We saw similar patterns of responses in both women and mice, supporting the use of mice for preclinical screening of novel maternal vaccines. Using a systems vaccinology approach in pregnancy demonstrated that pregnancy does not affect the initial response to vaccination and that studies in non-pregnant women can provide information about vaccine immunogenicity and potentially safety

Peripheral T cell lymphopenia in COVID-19: potential mechanisms and impact

Immunopathogenesis involving T lymphocytes, which play a key role in defence against viral infection, could contribute to the spectrum of COVID-19 disease and provide an avenue for treatment. To address this question, are view of clinical observational studies and autopsy data in English and Chinese languages was conducted with a search of registered clinical trials. Peripheral lymphopenia affecting CD4 and CD8 T cells was a striking feature of severe COVID-19 compared with non-severe disease. Autopsy data demonstrated infiltration of T cells into organs, particularly the lung. 74 clinical trials are on-going that could target T cell-related pathogenesis, particularly IL-6 pathways. SARS-CoV-2 infection interrupts T cell circulation in patients with severe COVID-19. This could be due to redistribution of T cells into infected organs, activation induced exhaustion, apoptosis or pyroptosis. Measuring T cell dynamics during COVID-19 will inform clinical risk-stratification of hospitalised patients and could identify those who would benefit most from 66treatments that target T cell

Basin-scale, integrated observations of the early 21st century multiyear drought in southeast Australia

The Murray-Darling Basin in southeast Australia is experiencing one of the most severe droughts observed recently in the world, driven by several years of rainfall deficits and record high temperatures. This paper provides new basin‐scale observations of the multiyear drought, integrated to a degree rarely achieved on such a large scale, to assess the response of water resources and the severity of the drought. A combination of Gravity Recovery and Climate Experiment (GRACE) data with in situ and modeled hydrological data shows the propagation of the water deficit through the hydrological cycle and the rise of different types of drought. Our observations show the rapid drying of soil moisture and surface water storages, which reached near‐stationary low levels only ∼2 years after the onset of the drought in 2001, with a loss of ∼80 and ∼12 km3 between January 2001 and January 2003, respectively. The multiyear drought has led to the almost complete drying of surface water resources which account for most of the water used for irrigation and domestic purposes. High correlation between observed groundwater variations and GRACE data substantiates the persistent reduction in groundwater storage, with groundwater levels still declining 6 years after the onset of the drought (groundwater loss of ∼104 km3 between 2001 and 2007). The hydrological drought continues even though the region returned to average annual rainfall during 2007

Tropical cyclones and the ecohydrology of Australia's recent continental-scale drought

The Big Dry, a recent drought over southeast Australia, began around 1997 and continued until 2011. We show that between 2002-2010, instead of a localized drought, there was a continent-wide reduction in water storage, vegetation and rainfall, spanning the northwest to the southeast of Australia. Trends in water storage and vegetation were assessed using Gravity Recovery and Climate Experiment (GRACE) and Normalized Difference Vegetation Index (NDVI) data. Water storage and NDVI are shown to be significantly correlated across the continent and the greatest losses of water storage occurred over northwest Australia. The frequency of tropical cyclones over northwest Australia peaked just prior to the launch of the GRACE mission in 2002. Indeed, since 1981, decade-scale fluctuations in tropical cyclone numbers coincide with similar variation in rainfall and vegetation over northwest Australia. Rainfall and vegetation in southeast Australia trended oppositely to the northwest prior to 2001. Despite differences between the northwest and southeast droughts, there is reason to believe that continental droughts may occur when the respective climate drivers align

Characterizing and minimizing the effects of noise in tide gauge time series: Relative and geocentric sea level rise around Australia

We quantify the rate of sea level rise around the Australian continent from an analysis of tidegauge and Global Positioning System (GPS) data sets. To estimate the underlying linear ratesof sea level change in the presence of significant interannual and