1,550 research outputs found
Metastable states of hydrogen: their geometric phases and flux densities
We discuss the geometric phases and flux densities for the metastable states
of hydrogen with principal quantum number n=2 being subjected to adiabatically
varying external electric and magnetic fields. Convenient representations of
the flux densities as complex integrals are derived. Both, parity conserving
(PC) and parity violating (PV) flux densities and phases are identified.
General expressions for the flux densities following from rotational invariance
are derived. Specific cases of external fields are discussed. In a pure
magnetic field the phases are given by the geometry of the path in magnetic
field space. But for electric fields in presence of a constant magnetic field
and for electric plus magnetic fields the geometric phases carry information on
the atomic parameters, in particular, on the PV atomic interaction. We show
that for our metastable states also the decay rates can be influenced by the
geometric phases and we give a concrete example for this effect. Finally we
emphasise that the general relations derived here for geometric phases and flux
densities are also valid for other atomic systems having stable or metastable
states, for instance, for He with n=2. Thus, a measurement of geometric phases
may give important experimental information on the mass matrix and the electric
and magnetic dipole matrices for such systems. This could be used as a check of
corresponding theoretical calculations of wave functions and matrix elements.Comment: 29 pages, 12 figure
Optimizing adipogenic transdifferentiation of bovine mesenchymal stem cells: a prominent role of ascorbic acid in FABP4 induction
Adipocyte differentiation of bovine adipose-derived stem cells (ASC) was induced by foetal bovine serum (FBS), biotin, pantothenic acid, insulin, rosiglitazone, dexamethasone and 3-isobutyl-1-methylxanthine, followed by incubation in different media to test the influence of ascorbic acid (AsA), bovine serum lipids (BSL), FBS, glucose and acetic acid on transdifferentiation into functional adipocytes. Moreover, different culture plate coatings (collagen-A, gelatin-A or poly-L-lysine) were tested. The differentiated ASC were subjected to Nile red staining, DAPI staining, immunocytochemistry and quantitative reverse transcription PCR (for NT5E, THY1, ENG, PDGFRα, FABP4, PPARγ, LPL, FAS, GLUT4). Nile red quantification showed a significant increase in the development of lipid droplets in treatments with AsA and BSL without FBS. The presence of BSL induced a prominent increase in FABP4 mRNA abundance and in FABP4 immunofluorescence signals in coincubation with AsA. The abundance of NT5E, ENG and THY1 mRNA decreased or tended to decrease in the absence of FBS, and ENG was additionally suppressed by AsA. DAPI fluorescence was higher in cells cultured in poly-L-lysine or gelatin-A coated wells. In additional experiments, the multi-lineage differentiation potential to osteoblasts was verified in medium containing ß-glycerophosphate, dexamethasone and 1,25-dihydroxyvitamin D3 using alizarin red staining. In conclusion, bovine ASC are capable of multi-lineage differentiation. Poly-L-lysine or gelatin-A coating, the absence of FBS, and the presence of BSL and AsA favour optimal transdifferentiation into adipocytes. AsA supports transdifferentiation via a unique role in FABP4 induction, but this is not linearly related to the primarily BSL-driven lipid accumulation
Universal Robotic Gripper based on the Jamming of Granular Material
Gripping and holding of objects are key tasks for robotic manipulators. The
development of universal grippers able to pick up unfamiliar objects of widely
varying shape and surface properties remains, however, challenging. Most
current designs are based on the multi-fingered hand, but this approach
introduces hardware and software complexities. These include large numbers of
controllable joints, the need for force sensing if objects are to be handled
securely without crushing them, and the computational overhead to decide how
much stress each finger should apply and where. Here we demonstrate a
completely different approach to a universal gripper. Individual fingers are
replaced by a single mass of granular material that, when pressed onto a target
object, flows around it and conforms to its shape. Upon application of a vacuum
the granular material contracts and hardens quickly to pinch and hold the
object without requiring sensory feedback. We find that volume changes of less
than 0.5% suffice to grip objects reliably and hold them with forces exceeding
many times their weight. We show that the operating principle is the ability of
granular materials to transition between an unjammed, deformable state and a
jammed state with solid-like rigidity. We delineate three separate mechanisms,
friction, suction and interlocking, that contribute to the gripping force.
Using a simple model we relate each of them to the mechanical strength of the
jammed state. This opens up new possibilities for the design of simple, yet
highly adaptive systems that excel at fast gripping of complex objects.Comment: 10 pages, 7 figure
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Kalapuya brunnea gen. & sp. nov. and its relationship to the other sequestrate genera in Morchellaceae
Kalapuya is described as a new, monotypic truffle genus in the Morchellaceae known only from the Pacific northwestern United States. Its relationship to other hypogeous genera within Morchellaceae is explored by phylogenetic analysis of the ribosomal LSU and EF1α protein coding region. The type species, K. brunnea, occurs in Douglas-fir forests up to about 50 y old on the west slope of the Cascade Range in Oregon and in the Coastal Ranges of Oregon and northern California. It has a roughened, warty, reddish brown to brown peridium, a solid whitish gleba that develops grayish brown mottling as the spores mature, and produces a cheesy-garlicky odor at maturity. Its smooth, ellipsoid spores resemble those of Morchella spp. but are much larger. The four hypogeous genera known in the Morchellaceae, Kalapuya, Fischerula, Imaia and Leucangium, are distinct from the epigeous genera Morchella and Verpa, but it is uncertain whether they resulted from a single transition to a hypogeous fruiting habit or from multiple independent transitions. Kalapuya, locally known as the Oregon brown truffle, has been commercially harvested for culinary use.Keywords: LSU rDNA, taxonomy, Pezizales, EF1α, Douglas-fir, Ascomycota, Fischerula, Morchellaceae, Leucangium, hypogeous fungus, truffl
Force-velocity-power and Force-pCa Relationships of Human Soleus Fibers After 17 Days of Bed Rest
Soleus muscle fibers from the rat display a reduction in peak power and Ca2+ sensitivity after hindlimb suspension. To examine human responses to non-weight bearing, we obtained soleus biopsies from eight adult men before and immediately after 17 days of bed rest (BR). Single chemically skinned fibers were mounted between a force transducer and a servo-controlled position motor and activated with maximal (isotonic properties) and/or submaximal (Ca2+ sensitivity) levels of free Ca2+. Gel electrophoresis indicated that all pre- and post-BR fibers expressed type I myosin heavy chain. Post-BR fibers obtained from one subject displayed increases in peak power and Ca2+ sensitivity. In contrast, post-BR fibers obtained from the seven remaining subjects showed an average 11% reduction in peak power (P \u3c 0.05), with each individual displaying a 7–27% reduction in this variable. Post-BR fibers from these subjects were smaller in diameter and produced 21% less force at the shortening velocity associated with peak power. However, the shortening velocity at peak power output was elevated 13% in the post-BR fibers, which partially compensated for their lower force. Post-BR fibers from these same seven subjects also displayed a reduced sensitivity to free Ca2+(P \u3c 0.05). These results indicate that the reduced functional capacity of human lower limb extensor muscles after BR may be in part caused by alterations in the cross-bridge mechanisms of contraction
Longitudinal Atomic Beam Spin Echo Experiments: A possible way to study Parity Violation in Hydrogen
We discuss the propagation of hydrogen atoms in static electric and magnetic
fields in a longitudinal atomic beam spin echo (lABSE) apparatus. Depending on
the choice of the external fields the atoms may acquire both dynamical and
geometrical quantum mechanical phases. As an example of the former, we show
first in-beam spin rotation measurements on atomic hydrogen, which are in
excellent agreement with theory. Additional calculations of the behaviour of
the metastable 2S states of hydrogen reveal that the geometrical phases may
exhibit the signature of parity-(P-)violation. This invites for possible future
lABSE experiments, focusing on P-violating geometrical phases in the lightest
of all atoms.Comment: 6 pages, 4 figure
Effect of temperature on aging and time-temperature superposition in nonergodic Laponite suspensions
We have studied the effect of temperature on aging dynamics of laponite
suspensions by carrying out the rheological oscillatory and creep experiments.
We observed that at higher temperatures the mechanism responsible for aging
became faster thereby shifting the evolution of elastic modulus to lower ages.
Significantly, in the creep experiments, all the aging time and the temperature
dependent strain data superposed to form a master curve. Possibility of such
superposition suggests that the rheological behavior depends on the temperature
and the aging time only through the relaxation processes and both the variables
do not affect the distribution but only the average value of relaxation times.
In addition, this procedure allows us to predict long time rheological behavior
by carrying out short time tests at high temperatures and small ages.Comment: 18 pages, 5 figure
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The hunted : commercially attractive truffles native to North America
Keywords: Tuber canaliculatum, Kalapuya brunnea, yellow-furrowed truffle, Oregon white truffles, Oregon black truffles, Pecan or Texas truffle, Leucangium carthusianum, Oregon brown truffles, Tuber gibbosum complex, giant Imaia, Imaia gigantea, Truffle harvesting, Tuber lyonii comple
Modeling mycorrhizal fungi dispersal by the mycophagous swamp wallaby (Wallabia bicolor)
Despite the importance of mammal-fungal interactions, tools to estimate the mammal-assisted dispersal distances of fungi are lacking. Many mammals actively consume fungal fruiting bodies, the spores of which remain viable after passage through their digestive tract. Many of these fungi form symbiotic relationships with trees and provide an array of other key ecosystem functions. We present a flexible, general model to predict the distance a mycophagous mammal would disperse fungal spores. We modelled the probability of spore dispersal by combining animal movement data from GPS-telemetry with data on spore gut-retention time. We test this model using an exemplar generalist mycophagist, the swamp wallaby (Wallabia bicolor). We show that swamp wallabies disperse fungal spores hundreds of metres—and occasionally up to 1265 m—from the point of consumption, distances that are ecologically significant for many mycorrhizal fungi. In addition to highlighting the ecological importance of swamp wallabies as dispersers of mycorrhizal fungi in eastern Australia, our simple modelling approach provides a novel and effective way of empirically describing spore dispersal by a mycophagous animal. This approach is applicable to the study of other animal-fungi interactions in other ecosystems.Funding provided by: Hermon Slade FoundationCrossref Funder Registry ID: http://dx.doi.org/10.13039/501100001109Award Number: HSF08-6Funding provided by: Australian Research CouncilCrossref Funder Registry ID: http://dx.doi.org/10.13039/501100000923Award Number: DP0557022Methods are described in the published article
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