The challenge hypothesis revisited: Focus on reproductive experience and neural mechanisms.

Our review focuses on findings from mammals as part of a Special Issue "30th Anniversary of the Challenge Hypothesis". Here we put forth an integration of the mechanisms through which testosterone controls territorial behavior and consider how reproductive experience may alter these mechanisms. The emphasis is placed on the function of socially induced increases in testosterone (T) pulses, which occur in response to social interactions, as elegantly developed by Wingfield and colleagues. We focus on findings from the monogamous California mouse, as data from this species shows that reproductive status is a key factor influencing social interactions, site fidelity, and vigilance for offspring defense. Specifically, we examine differences in T pulses in sexually naïve versus sexually experienced pair bonded males. Testosterone pulses influence processes such as social decision making, the winner-challenge effect, and location preferences through rewarding effects of T. We also consider how social and predatory vigilance contribute to T pulses and how these interactions contribute to a territory centered around maximizing reproduction. Possible underlying mechanisms for these effects include the nucleus accumbens (rewarding effects of testosterone), hippocampus (spatial memories for territories), and the bed nucleus of the stria terminalis (social vigilance). The development of the challenge effect has provided an ideal framework for understanding the complex network of behavioral, environmental, physiological and neural mechanisms that ultimately relates to competition and territoriality across taxa. The opportunity to merge research on the challenge effect using both laboratory and field research to understand social behavior is unparalleled

Mild acute stress improves response speed without impairing accuracy or interference control in two selective attention tasks: Implications for theories of stress and cognition.

Acute stress is generally thought to impair performance on tasks thought to rely on selective attention. This effect has been well established for moderate to severe stressors, but no study has examined how a mild stressor-the most common type of stressor-influences selective attention. In addition, no study to date has examined how stress influences the component processes involved in overall selective attention task performance, such as controlled attention, automatic attentional activation, decision-making, and motor abilities. To address these issues, we randomly assigned 107 participants to a mild acute stress or control condition. As expected, the mild acute stress condition showed a small but significant increase in cortisol relative to the control condition. Following the stressor, we assessed attention with two separate flanker tasks. One of these tasks was optimized to investigate component attentional processes using computational cognitive modeling, whereas the other task employed mouse-tracking to illustrate how response conflict unfolded over time. The results for both tasks showed that mild acute stress decreased response time (i.e., increased response speed) without influencing accuracy or interference control. Further, computational modeling and mouse-tracking analyses indicated that these effects were due to faster motor action execution time for chosen actions. Intriguingly, however, cortisol responses were unrelated to any of the observed effects of mild stress. These results have implications for theories of stress and cognition, and highlight the importance of considering motor processes in understanding the effects of stress on cognitive task performance

A High-Resolution Hubble Space Telescope Study of Apparent Lyman Continuum Leakers at z∼3z\sim3

We present $U_{336}V_{606}J_{125}H_{160}$ follow-up $HST$ observations of 16 $z\sim3$ candidate LyC emitters in the HS1549+1919 field. With these data, we obtain high spatial-resolution photometric redshifts of all sub-arcsecond components of the LyC candidates in order to eliminate foreground contamination and identify robust candidates for leaking LyC emission. Of the 16 candidates, we find one object with a robust LyC detection that is not due to foreground contamination. This object (MD5) resolves into two components; we refer to the LyC-emitting component as MD5b. MD5b has an observed 1500\AA\ to 900\AA\ flux-density ratio of $(F_{UV}/F_{LyC})_{obs}=4.0\pm2.0$, compatible with predictions from stellar population synthesis models. Assuming minimal IGM absorption, this ratio corresponds to a relative (absolute) escape fraction of $f_{esc,rel}^{MD5b}=75-100$% ($f_{esc,abs}^{MD5b}=14-19$%). The stellar population fit to MD5b indicates an age of $\lesssim50$Myr, which is in the youngest 10% of the $HST$ sample and the youngest third of typical $z\sim3$ Lyman break galaxies, and may be a contributing factor to its LyC detection. We obtain a revised, contamination-free estimate for the comoving specific ionizing emissivity at $z=2.85$, indicating (with large uncertainties) that star-forming galaxies provide roughly the same contribution as QSOs to the ionizing background at this redshift. Our results show that foreground contamination prevents ground-based LyC studies from obtaining a full understanding of LyC emission from $z\sim3$ star-forming galaxies. Future progress in direct LyC searches is contingent upon the elimination of foreground contaminants through high spatial-resolution observations, and upon acquisition of sufficiently deep LyC imaging to probe ionizing radiation in high-redshift galaxies.Comment: 31 pages, 5 tables, 19 figures. Accepted to ApJ. Version with full-resolution figures is available at: http://www.astro.ucla.edu/~aes/Mostardi_HST_LyC.pd

Sexual Dimorphism in the Brain of the Monogamous California Mouse (Peromyscus californicus)

Sex differences in behavior and morphology are usually assumed to be stronger in polygynous species compared to monogamous species. A few brain structures have been identified as sexually dimorphic in polygynous rodent species, but it is less clear whether these differences persist in monogamous species. California mice are among the 5% of mammals that are considered to be monogamous and as such provide an ideal model to examine sexual dimorphism in neuroanatomy. In the present study we compared the volume of hypothalamic and limbic associated regions in female and male California mice for sexual dimorphism. We also used tyrosine hydroxylase immunohistochemistry to compare the number of dopamine neurons in the ventral tegmental area (VTA) in female and male California mice. Additionally, tract tracing was used to accurately delineate the boundaries of the VTA. The total volume of the sexually dimorphic nucleus of the preoptic area (SDN-POA), the principal nucleus of the bed nucleus of the stria terminalis, and the posterodorsal medial amygdala (MEApd) was larger in males compared to females. In the SDN-POA we found that the magnitude of sex differences in the California mouse were intermediate between the large differences observed in promiscuous meadow voles and rats and the absence of significant differences in monogamous prairie voles. However, the magnitude of sex differences in medial amygdala and the bed nucleus of the stria terminalis were comparable to polygynous species. No sex differences were observed in the volume of the whole brain, the VTA, the nucleus accumbens or the number of TH-ir neurons in the VTA. These data show that despite a monogamous social organization, sexual dimorphisms that have been reported in polygynous rodents extend to California mice. Our data suggest that sex differences in brain structures such as the SDN-POA persist across species with different social organizations and may be an evolutionarily conserved characteristic of mammalian brains

Sex differences in stress-induced social withdrawal: role of brain derived neurotrophic factor in the bed nucleus of the stria terminalis

Depression and anxiety disorders are more common in women than men, and little is known about the neurobiological mechanisms that contribute to this disparity. Recent data suggest that stress-induced changes in neurotrophins have opposing effects on behavior by acting in different brain networks. Social defeat has been an important approach for understanding neurotrophin action, but low female aggression levels in rats and mice have limited the application of these methods primarily to males. We examined the effects of social defeat in monogamous California mice (Peromyscus californicus), a species in which both males and females defend territories. We demonstrate that defeat stress increases mature brain-derived neurotrophic factor (BDNF) protein but not mRNA in the bed nucleus of the stria terminalis (BNST) in females but not males. Changes in BDNF protein were limited to anterior subregions of the BNST, and there were no changes in the adjacent nucleus accumbens (NAc). The effects of defeat on social withdrawal behavior and BDNF were reversed by chronic, low doses of the antidepressant sertraline. However, higher doses of sertraline restored social withdrawal and elevated BDNF levels. Acute treatment with a low dose of sertraline failed to reverse the effects of defeat. Infusions of the selective tyrosine-related kinase B receptor (TrkB) antagonist ANA-12 into the anterior BNST specifically increased social interaction in stressed females but had no effect on behavior in females naïve to defeat. These results suggest that stress-induced increases in BDNF in the anterior BNST contribute to the exaggerated social withdrawal phenotype observed in females

Sex Differences in Social Interaction Behavior Following Social Defeat Stress in the Monogamous California Mouse (Peromyscus californicus)

Stressful life experiences are known to be a precipitating factor for many mental disorders. The social defeat model induces behavioral responses in rodents (e.g. reduced social interaction) that are similar to behavioral patterns associated with mood disorders. The model has contributed to the discovery of novel mechanisms regulating behavioral responses to stress, but its utility has been largely limited to males. This is disadvantageous because most mood disorders have a higher incidence in women versus men. Male and female California mice (Peromyscus californicus) aggressively defend territories, which allowed us to observe the effects of social defeat in both sexes. In two experiments, mice were exposed to three social defeat or control episodes. Mice were then behaviorally phenotyped, and indirect markers of brain activity and corticosterone responses to a novel social stimulus were assessed. Sex differences in behavioral responses to social stress were long lasting (4 wks). Social defeat reduced social interaction responses in females but not males. In females, social defeat induced an increase in the number of phosphorylated CREB positive cells in the nucleus accumbens shell after exposure to a novel social stimulus. This effect of defeat was not observed in males. The effects of defeat in females were limited to social contexts, as there were no differences in exploratory behavior in the open field or light-dark box test. These data suggest that California mice could be a useful model for studying sex differences in behavioral responses to stress, particularly in neurobiological mechanisms that are involved with the regulation of social behavior

Conserved transcriptomic profiles underpin monogamy across vertebrates

Social monogamy, typically characterized by the formation of a pair bond, increased territorial defense, and often biparental care, has independently evolved multiple times in animals. Despite its independent origins, several homologous brain regions, neuropeptides and their receptors have been shown to play a conserved role in regulating social affiliation and parental care, but little is known is known about the neuromolecular mechanisms underlying monogamy on a genomic scale. Here, we compare neural transcriptomes of reproductive males in monogamous and nonmonogamous species pairs of Peromyscus mice, Microtus voles, parid songbirds, dendrobatid frogs, and Xenotilapia species of cichlid fishes. We find that while evolutionary divergence time between species or clades did not explain gene expression similarity, characteristics of mating system correlated with neural gene expression patterns and neural gene expression varies concordantly across vertebrates when species transition to monogamy. Our study provides evidence of a universal transcriptomic mechanism underlying the evolution of monogamy in vertebrates

Health Aspects of the Pre-Departure Phase of Migration

In the second article in a six-part PLoS Medicine series on Migration & Health, Brian Gushulak and Douglas MacPherson discuss the pre-departure phase of migration and the specific health risks and policy needs associated with this phase