52 research outputs found
Impaired IFN-γ production and proliferation of NK cells in Multiple Sclerosis
NK cells are multicompetent lymphocytes of the innate immune system with a central role in host defense and immune regulation. Studies in experimental animal models of multiple sclerosis (MS) provided evidence for both pathologic and protective effects of NK cells. Humans harbor two functionally distinct NK-cell subsets exerting either predominantly cytotoxic (CD56dimCD16+) or immunoregulatory (CD56brightCD16−) functions. We analyzed these two subsets and their functions in the peripheral blood of untreated patients with relapsing-remitting MS compared with healthy blood donors. While ex vivo frequencies of CD56brightCD16− and CD56dimCD16+ NK cells were similar in patients and controls, we found that cytokine-driven in vitro accumulation and IFN-γ production of CD56brightCD16− NK cells but not of their CD56dimCD16+ counterparts were substantially diminished in MS. Impaired expansion of CD56brightCD16− NK cells was cell intrinsic because the observed effects could be reproduced with purified NK cells in an independent cohort of patients and controls. In contrast, cytolytic NK-cell activity toward the human erythromyeloblastoid leukemia cell line K562, the allogeneic CD4+ T cell line CEM and allogeneic primary CD4+ T-cell blasts was unchanged. Thus, characteristic functions of CD56brightCD16− NK cells, namely cytokine-induced NK cell expansion and IFN-γ production, are compromised in the NK cell compartment of MS patient
High-Quality Draft Genome Sequences of Four Lignocellulose-Degrading Bacteria Isolated from Puerto Rican Forest Soil: Gordonia sp., Paenibacillus sp., Variovorax sp., and Vogesella sp.
Here, we report the high-quality draft genome sequences of four phylogenetically diverse lignocellulose-degrading bacteria isolated from tropical soil (Gordonia sp., Paenibacillus sp., Variovorax sp., and Vogesella sp.) to elucidate the genetic basis of their ability to degrade lignocellulose. These isolates may provide novel enzymes for biofuel production
Preterm neonatal morbidity and mortality by gestational age: a contemporary cohort
Although preterm birth less than 37 weeks gestation is the leading cause of neonatal morbidity and mortality in the United States, the majority of data regarding preterm neonatal outcomes come from older studies, and many reports have been limited to only very preterm neonates. Delineation of neonatal outcomes by delivery gestational age is needed to further clarify the continuum of mortality and morbidity frequencies among preterm neonates
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Comprehensive approach to diagnosis and treatment of newly diagnosed primary CNS lymphoma.
Primary central nervous system lymphoma (PCNSL) is a rare form of non-Hodgkin lymphoma that affects the brain parenchyma, spinal cord, eyes, and cerebrospinal fluid without evidence of systemic, non-CNS involvement. PCNSL is uncommon and only a few randomized trials have been completed in the first-line setting. Over the past decades, the prognosis of PCNSL has improved, mainly due to the introduction and widespread use of high-dose methotrexate, which is now the backbone of all first-line treatment polychemotherapy regimens. Despite this progress, durable remission is recorded in only 50% of patients, and therapy can be associated with significant late neurotoxicity. Here, we overview the epidemiology, clinical presentation, staging evaluation, prognosis, and current up-to-date treatment of immunocompetent PCNSL patients
EHV-1 and Platelets: Raw Data
Data provided is the raw data used to derive the results presented in the figures of the paper. The data is organized by the Figure number and consists of flow cytometric files generated by a BD FACSCAN flow cytometer and a tab-delimited file containing information (treatments etc) for each FACS file. Each figure also includes jpg files of the analyzed FACS data used to compile the figures. The jpgs were generated in FlowJo software, version 10. At the root level, there is a Prism file (PZF; GraphPad Prism for Mac, Version 7.0) used to generate the Figures and statistically analyze the data.Harold M. Zweig Memorial Fund for Researc
Correction: Equid Herpesvirus Type 1 Activates Platelets.
[This corrects the article DOI: 10.1371/journal.pone.0122640.]
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