81 research outputs found
Comparative study between immunofluorescence and coproculture in the diagnosis of intestinal infections by enteropathogenic Escherichia coli
A new Escherichia coli serotype causing experimental kerato-conjunctivitis in the guinea-pig (culture 412-DEC-66). (Preliminary report)
ocorrência de Campylobacter jejuni em animais do zoológico
Fifty fecal specimens from animals of the São Paulo City Zoo were cultured for Campylobacter jejuni. The organism was isolated from 4 out 20 primates and it was not found in the other mammals and birds assayed in this study.Foram estudadas 50 amostras de fezes obtidas de diversas espécies animais da Fundação Parque Zoológico de São Paulo. C. jejuni foi isolado em 4 dos 20 primatas estudados, não sendo encontrado nas fezes de outros mamÃferos e aves ingressados neste estudo
Prevalence of diarrheagenic Escherichia coli in children with diarrhea in Salvador, Bahia, Brazil
We report the frequency of the different diarrheagenic Escherichia coli (DEC) categories isolated from children with acute endemic diarrhea in Salvador, Bahia. The E. coli isolates were investigated by colony blot hibridization whit the following genes probes: eae, EAF, bfpA, Stx1, Stx2, ST-Ih, ST-Ip, LT-I, LT-II, INV, and EAEC, as virulence markers to distinguish typical and atypical EPEC, EHEC/STEC, ETEC, EIEC, and EAEC. Seven of the eight categories of DEC were detected. The most frequently isolated was atypical EPEC (10.1%) followed by ETEC (7.5%), and EAEC (4.2%). EHEC, STEC, EIEC, and typical EPEC were each detected once. The strains of ETEC, EAEC, and atypical EPEC belonged to a wide variety of serotypes. The serotypes of the others categories were O26:H11 (EHEC), O21:H21 (STEC), O142:H34 (typical EPEC), and O?H55 (EIEC). We also present the clinical manifestations and other pathogenic species observed in children with DEC. This is the first report of EHEC and STEC in Salvador, and one of the first in Brazil.Instituto Butantan Laboratório Especial de MicrobiologiaUniversidade Federal de São Paulo (UNIFESP) Imunologia e Parasitologia Departamento de MicrobiologiaRobert Koch Institut Division of Emerging Bacterial PathogensInstituto de Saúde ColetivaUniversidade Federal da Bahia Departamento de PediatriaUNIFESP, Imunologia e Parasitologia Depto. de MicrobiologiaSciEL
Polyclonal anti-intimin antibody: immunological characterization and its use in EPEC and EHEC diagnosis
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