Genetic resistance to powdery mildew in common bean

Powdery mildew can cause severe yield losses in bean crops. Limited information about resistance sources, and nature and inheritance of resistance are available to bean breeders and plant pathologist. Sources of resistance were searched in seedling tests under controlled conditions in 44 well-known genotypes and in a Spanish germplasm core collection consisting on 201 accessions. A 0-4 scale was used to describe the infection types (IT) observed. Only six out of the 245 evaluated genotypes showed a complete resistance (IT0) without visible symptoms on the leaves: Amanda, Belneb, Cornell 49242, Negro San Luis, Porrillo Sintetico and the local accession BGE003161. Inheritance of resistance was studied in F and F segregating populations. Observed reactions in the five segregating populations fitted to Mendelian ratios with different modes of inheritance. Results revealed that cultivar Porrillo Sintetico carries two dominant and independent resistance genes: one gene conferring complete resistance (IT0), and another gene conferring IT3, characterized by a moderate mycelial development on the leaves. Both genes show a dominant epistatic relationship. Inheritance of response to powdery mildew in cv. Cornell 49242 was similar to cv. Porrillo Sintetico although the correspondence with the genes described in Porrillo Sintetico was not established. Line X2776 carries one dominant gene conferring IT3, and shares this gene with cv. Porrillo Sintetico. In cv. Amanda, two complementary genes appear to be involved in resistance to this fungus. This information will be relevant for the implementation of breeding programs focused on the development of cultivars carrying genetic resistance to powdery mildew

Transcriptional Regulation of Sorghum Stem Composition : Key Players Identified Through Co-expression Gene Network and Comparative Genomics Analyses

Most sorghum biomass accumulates in stem secondary cell walls (SCW). As sorghum stems are used as raw materials for various purposes such as feed, energy and fiber reinforced polymers, identifying the genes responsible for SCW establishment is highly important. Taking advantage of studies performed in model species, most of the structural genes contributing at the molecular level to the SCW biosynthesis in sorghum have been proposed while their regulatory factors have mostly not been determined. Validation of the role of several MYB and NAC transcription factors in SCW regulation in Arabidopsis and a few other species has been provided. In this study, we contributed to the recent efforts made in grasses to uncover the mechanisms underlying SCW establishment. We reported updated phylogenies of NAC and MYB in 9 different species and exploited findings from other species to highlight candidate regulators of SCW in sorghum. We acquired expression data during sorghum internode development and used co-expression analyses to determine groups of co-expressed genes that are likely to be involved in SCW establishment. We were able to identify two groups of co-expressed genes presenting multiple evidences of involvement in SCW building. Gene enrichment analysis of MYB and NAC genes provided evidence that while NAC SECONDARY WALL THICKENING PROMOTING FACTOR NST genes and SECONDARY WALL-ASSOCIATED NAC DOMAIN PROTEIN gene functions appear to be conserved in sorghum, NAC master regulators of SCW in sorghum may not be as tissue compartmentalized as in Arabidopsis. We showed that for every homolog of the key SCW MYB in Arabidopsis, a similar role is expected for sorghum. In addition, we unveiled sorghum MYB and NAC that have not been identified to date as being involved in cell wall regulation. Although specific validation of the MYB and NAC genes uncovered in this study is needed, we provide a network of sorghum genes involved in SCW both at the structural and regulatory levels

Quest for barley canopy architecture genes in the hortillus population and whealbi germplasm collection

Barley grains are predominantly used for animal feed and malting, and breeding traditionally focused on increase of grain yield by partitioning biomass from straw to grains. The increasing demand for renewable energy sources makes straw, and specially barley straw characterized by the largest content of carbohydrates among the cereals, a valuable product for its potential conversion into biofuels and other products. The BarPLUS project aims at finding genes, alleles and candidate lines related to barley canopy architecture and photosynthesis, to maximize barley biomass and yield (https://barplus.wordpress.com/). In this framework, our research group focuses on identifying genes and alleles controlling tillering, leaf size and leaf angle traits in barley by exploiting both induced and natural allelic variation. Using a forward genetics approach, we screened the HorTILLUS population (Szurman-Zubrzycka et al., 2018) under both field and controlled conditions, identifying 5 mutants with increased tillering and/or erect leaves. After crossing with four reference cultivars, pools of F2 wild-type and mutant plants were selected to map and identify the underlying genes by exome sequencing (Mascher et al., 2014). In parallel, TILLING of the HorTILLUS population identified four lines carrying mutations in the LBO (Lateral branching oxidoreductase) gene involved in tiller number. In order to explore also natural genetic variation, we are taking advantage of the \u2018WHEALBI\u2019 germplasm collection, which includes 403 exome sequenced diverse accessions (BustosKorts et al., 2019): a field trial on a subset of 240 lines (Fiorenzuola d\u2019Arda, Italy) allowed us to conduct a preliminary genome wide association study based on high-throughput phenotyping for leaf angle (PocketPlant3D smartphone app) and quantitative image-analysis for leaf size. Results will be compared with those from a greenhouse experiment on the same 240 accessions to analyze a wide range of morphological traits and identify associated markers and genomic regions

Genetic resistance to powdery mildew in common bean

Powdery mildew can cause severe yield losses in bean crops. Limited information about resistance sources, and nature and inheritance of resistance are available to bean breeders and plant pathologist. Sources of resistance were searched in seedling tests under controlled conditions in 44 well-known genotypes and in a Spanish germplasm core collection consisting on 201 accessions. A 0-4 scale was used to describe the infection types (IT) observed. Only six out of the 245 evaluated genotypes showed a complete resistance (IT0) without visible symptoms on the leaves: Amanda, Belneb, Cornell 49242, Negro San Luis, Porrillo Sintetico and the local accession BGE003161. Inheritance of resistance was studied in F 2 and F 2:3 segregating populations. Observed reactions in the five segregating populations fitted to Mendelian ratios with different modes of inheritance. Results revealed that cultivar Porrillo Sintetico carries two dominant and independent resistance genes: one gene conferring complete resistance (IT0), and another gene conferring IT3, characterized by a moderate mycelial development on the leaves. Both genes show a dominant epistatic relationship. Inheritance of response to powdery mildew in cv. Cornell 49242 was similar to cv. Porrillo Sintetico although the correspondence with the genes described in Porrillo Sintetico was not established. Line X2776 carries one dominant gene conferring IT3, and shares this gene with cv. Porrillo Sintetico. In cv. Amanda, two complementary genes appear to be involved in resistance to this fungus. This information will be relevant for the implementation of breeding programs focused on the development of cultivars carrying genetic resistance to powdery mildew. © 2012 Springer Science+Business Media B.V.This work was supported by grant RTA2009-093 from INIA-Ministerio de Ciencia y Tecnología, Spain. Noemí Trabanco was the recipient of a salary fellowship from Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA, Spain).Peer Reviewe

Uma nova perspectiva para as altitudes físicas no Brasil

The physical heights definition of heights, proposed by Helmert in 1890 is one of the commonly used heights systems in practice. In Helmert’s definition, the mean value of gravity along plumbline is computed by simplifying the topography with a Bouguer shell containing masses with mean density value. Although this approximation might be accurate enough many purposes, a more rigorous definition can be determined by considering the effects of terrain, topographic mass density variation, and masses contained in the geoid the mean gravity value along the plumbline. The purpose of this paper is to compute the corrections for the Helmert’s definition of the orthometric heights to obtain the rigorous orthometric heights in the state of São Paulo and adjacent states and to evaluate these corrections. The heights system used in Brazil (until July 2018) and some South American countries is normal-orthometric heights, therefore the corrections needs to be applied accordingly. Our numerical results show that there are significant differences between the normal-orthometric and rigorous orthometric heights, with maximum values of ~ 0.4 m, minimum of ~-0.8 m and mean value of ~-0.32 m. There are larger differences between normal-orthometric and normal height than the ones between normal and rigorous definition of orthometric heights251COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPES88881.132305/2016-1A definição das altitudes físicas proposta por Helmert em 1890 é um dos sistemas de altitude comumente utilizado na prática. Na definição de Helmert o valor médio da gravidade ao longo do fio de prumo é calculado simplificando a topografia, com Bouguer Shell, contendo um valor médio para a densidade de massas. Embora esta aproximação possa ser bastante acurada para muitos propósitos, uma definição mais rigorosa pode ser determinada considerando os efeitos do terreno, a variação da densidade de massa topográfica e as massas contidas no geoide, para o valor médio da gravidade ao longo do fio de prumo. O objetivo deste artigo é calcular as correções para a altitude ortométrica de Helmert para obter-se a altitude ortométrica rigorosa para o estado de São Paulo e estados vizinhos, além de avaliar estas correções. No entanto, o sistema de altitude utilizado no Brasil (até julho de 2018) e em alguns países da América do Sul é a normalortométrica, portanto as correções precisam ser aplicadas corretamente. Os resultados numéricos mostram que existem diferenças significativas entre as altitudes normal-ortométrica e rigorosa, com valores máximos de ~ 0,4 m, mínimo de ~ -0,8 m e valor médio de ~ -0,32 m. Encontram-se as maiores diferenças entre as altitudes normal-ortométrica e normal do que entre a altitude normal e a definição mais rigorosa de altitud

Late-stage difluoromethylation: concepts, developments and perspective

This review describes the recent advances made in difluoromethylation processes based on X–CF2H bond formation where X is C(sp), C(sp2), C(sp3), O, N or S, a field of research that has benefited from the invention of multiple difluoromethylation reagents. The last decade has witnessed an upsurge of metal-based methods that can transfer CF2H to C(sp2) sites both in stoichiometric and catalytic mode. Difluoromethylation of C(sp2)–H bond has also been accomplished through Minisci-type radical chemistry, a strategy best applied to heteroaromatics. Examples of electrophilic, nucleophilic, radical and cross-coupling methods have appeared to construct C(sp3)–CF2H bonds, but cases of stereoselective difluoromethylation are still limited. In this sub-field, an exciting departure is the precise site-selective installation of CF2H onto large biomolecules such as proteins. The formation of X–CF2H bond where X is oxygen, nitrogen or sulfur is conventionally achieved upon reaction with ClCF2H; more recently, numerous protocols have achieved X–H insertion with novel non-ozone depleting difluorocarbene reagents. All together, these advances have streamlined access to molecules of pharmaceutical relevance, and generated interest for process chemistry