50 research outputs found

    Quest for barley canopy architecture genes in the hortillus population and whealbi germplasm collection

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    Barley grains are predominantly used for animal feed and malting, and breeding traditionally focused on increase of grain yield by partitioning biomass from straw to grains. The increasing demand for renewable energy sources makes straw, and specially barley straw characterized by the largest content of carbohydrates among the cereals, a valuable product for its potential conversion into biofuels and other products. The BarPLUS project aims at finding genes, alleles and candidate lines related to barley canopy architecture and photosynthesis, to maximize barley biomass and yield (https://barplus.wordpress.com/). In this framework, our research group focuses on identifying genes and alleles controlling tillering, leaf size and leaf angle traits in barley by exploiting both induced and natural allelic variation. Using a forward genetics approach, we screened the HorTILLUS population (Szurman-Zubrzycka et al., 2018) under both field and controlled conditions, identifying 5 mutants with increased tillering and/or erect leaves. After crossing with four reference cultivars, pools of F2 wild-type and mutant plants were selected to map and identify the underlying genes by exome sequencing (Mascher et al., 2014). In parallel, TILLING of the HorTILLUS population identified four lines carrying mutations in the LBO (Lateral branching oxidoreductase) gene involved in tiller number. In order to explore also natural genetic variation, we are taking advantage of the \u2018WHEALBI\u2019 germplasm collection, which includes 403 exome sequenced diverse accessions (BustosKorts et al., 2019): a field trial on a subset of 240 lines (Fiorenzuola d\u2019Arda, Italy) allowed us to conduct a preliminary genome wide association study based on high-throughput phenotyping for leaf angle (PocketPlant3D smartphone app) and quantitative image-analysis for leaf size. Results will be compared with those from a greenhouse experiment on the same 240 accessions to analyze a wide range of morphological traits and identify associated markers and genomic regions

    One Health at the animal-human-environmental interface in Oloisukut conservancy, Narok County

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    Presented by Erastus Mulinge, Zipporah Gitau and Christina Trabanco at the Kenya One Health conference, 6-8 December 202

    Hydrodifluoromethylation of alkenes with difluoroacetic acid

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    A facile method for the regioselective hydrodifluoromethylation of alkenes is reported using difluoroacetic acid and phenyliodine(III) diacetate in tetrahydrofuran under visible‐light activation. This metal‐free approach stands out as it uses inexpensive reagents, does not require a photocatalyst, and displays broad functional group tolerance. The procedure is also operationally simple and scalable, and provides access in one step to high‐value building blocks for application in medicinal chemistry

    Anthropometric characteristics and somatotype of professional soccer players by position

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    The anthropometric characteristics are decisive for an optimal physical level and, therefore, a good level in the game; and they can be different depending on the game position. The aim of this study was to identify the physical characteristics, body composition and somatotype of professional soccer players and to verify differences according to their playing positions: goalkeepers, defenders, forwards and midfielders. The measurements were performed on 57 male players of a soccer team of the Spanish Football League One. Twenty seven anthropometric variables were measured (height and body weight, four bone breadths, eleven girths and ten skinfolds) and the Bioelectrical Impedance Analysis was also performed. The percentage of body fat has been determined from 11 different equations. Goalkeepers showed the highest weight (80.2 ± 3.2 kg), supraespinal (10.5 ± 3.8 mm) and abdominal (15.6 ± 3.5 mm) skinfolds than others positions. In relation to body fat percentages, similar results were obtained from the equations of Jackson-Pollock (from 3 and 7 skinfolds), Carter, Withers, and Heyward and Stolarczyk (mean value 7.8 ± 1.5%). Higher results were obtained from the other equations applied. Differences among positions were also found concerning body composition; goalkeepers showed the highest body fat percentage (9.4 ± 1.4%). Mean somatotype was also different among positions; goalkeepers and forwards presented a balanced mesomorph somatotype while defenders and midfielders showed an ecto-mesomorph one. The differences in morphological characteristics according to the team position were notice only in goalkeepers, especially regarding their weight, abdominal and supraespinale skinfolds and the percentage of fat tissue

    Bench-stable transfer reagent facilitates the generation of trifluoromethyl-sulfonimidamides

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    Sulfonimidamides are an emerging bioisosteric replacement in medicinal chemistry projects, and therefore new chemistries are necessary to access this functionality. The general synthesis of CF3-sulfonimidamides from an activated bench-stable transfer reagent is described. A diverse reaction scope is demonstrated, with a wide range of nucleophilic amines being tolerated in this transformation. The CF3-sulfonimidamides obtained contain an additional diversity point, in the form a protected imine, that could be unmasked to allow late stage modifications

    Bench-stable transfer reagent facilitates the generation of trifluoromethyl-sulfonimidamides

    No full text
    Sulfonimidamides are an emerging bioisosteric replacement in medicinal chemistry projects, and therefore new chemistries are necessary to access this functionality. The general synthesis of CF3-sulfonimidamides from an activated bench-stable transfer reagent is described. A diverse reaction scope is demonstrated, with a wide range of nucleophilic amines being tolerated in this transformation. The CF3-sulfonimidamides obtained contain an additional diversity point, in the form a protected imine, that could be unmasked to allow late stage modifications

    Magnet alignment on a common girder: development of a length artefact for measurement accuracy improvement

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    The tolerances for the alignment of the magnets on the girders of the proposed particle accelerator of the Brazilian Synchrotron Light Laboratory (LNLS), Sirius, are as small as 40 ÎŒm for translations and 0.3 mrad for rotations. The functional axis of the magnets is measured by the vibrating wire technique, which employs conductive wires of diameters of approx. 0.1 mm. Since the alignment has to be performed targeting these magnetic axes, rather than their geometric centrelines, non-contact measuring sensors mounted on a coordinate measuring machine have been chosen to measure the relative deviations between magnets. To better the measurement accuracy for that specific measuring task, to allow interim checks on multiple coordinate measuring systems, and to provide effective traceability to the SI unit of length, a multipurpose geometrical artefact has been devised. The reasoning behind this development and the first measurement results are described in this paper.50536

    First steps using two GPS satellites for monitoring the dynamic behavior of a small concrete highway bridge

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    This work presents the results of global positioning system (GPS) data processing using the phase residuals method (PRM)L1 carrier phase from two satellitesto monitor the dynamic behavior of a small concrete bridge. The bridge tested, the Jaguari Bridge, is a small, curvd, reinforced concrete bridge. The bridge over the Jaguari River is a reinforced concrete bridge built in 1999, located on FernAo Dias Highway (BR 381), positioned at Kilometer 946, Minas Gerais, Brazil. The small concrete bridge was chosen for this study because its construction type is found in great numbers throughout Brazil. In parallel, there was a significant increase of pathologies in these structures as a result of lack of maintenance procedures. The detection of small vibrations of spans, for example, which is a good indicator of the health of a structure, can be monitored by GPS. The challenge in this case is trying to detect with GPS the dynamic displacement that has an amplitude close to 5 mm. Application of the PRM on GPS data allowed the detection of this very small dynamic vibration. In addition, this is the first case of success with applying GPS as a tool for monitoring the dynamic behavior of small concrete bridges. The experiment consisted of sessions conducted during 2 days and used two GPS receivers (with a 100-Hz recording rate) over the central span of the Jaguari Bridge in 2014. The continuous wavelet transform (CWT) was used as a filter technique to analyze the frequencies generated by residues from double-difference data processing1423CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ422439-08-
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