The mosaic leafhopper Orientus ishidae: host plants, spatial distribution, infectivity, and transmission of 16SrV phytoplasmas to vines

Orientus ishidae (Matsumura) is an Asian species introduced into Europe and recently associated with 16SrV phytoplasmas, related to grapevine “flavescence dorée”. Its life cycle, host plants, spatial distribution, infection and vector capability have been investigated in vine-growing areas of Piedmont, NW Italy. The spatial distribution of adults in vineyards was studied by applying interpolation methods to trap capture data. Insects were subject to molecular analyses to verify phytoplasma presence and identity. DNA extraction and PCR were made to detect 16SrV phytoplasmas. Transmission experiments were set up, using different sources for phytoplasma acquisition, and two plant species and an artificial diet for inoculation. Whole mount in situ hybridization was made to detect phytoplasmas in the salivary glands of adults. In the vineyard agro-ecosystem, 19 plant species (11 families), mainly broadleaf trees and shrubs, were recognized as host plants of the insect. Adults were more abundant on putative host plants than on grapevines, with a clear clustering at the edges of vineyards, and without a massive intrusion into the vineyard from outside. 16SrV phytoplasmas were detected only in adults captured with yellow sticky traps (20 out of 188 tested). The transmission of 16SrV phytoplasmas was successful after phytoplasma acquisition from infected broad bean and inoculation on grapevine

Hubungan Karakteristik dengan Partisipasi Masyarakat dalam Pengelolaan Sampah di Lingkungan III dan V Kelurahan Bagan Deli Kecamatan Medan Belawan Tahun 2012

Relationship of Responden Characteristics (Education, Income, Occupation Knowledge and Attitude) And The Management Of Solid Waste Management Program In Area III and V Bagan Deli, Medan Belawan sub-district. Solid waste management program is one of the programs of Ministry of Sea and Fishery in Medan Belawan sub-district. On its implementation, solid waste management program in area III and V yet done well. Lack of the participation of the society on solid waste management may be caused by less understanding on sorting out the rubbish of household. The objective of this research is to analyze the relationship of responden characteristics (education, income, occupation knowledge and attitude) and the management of solid waste management program in area III and V Bagan Deli, Medan Belawan sub-district. This research was analytical survey with cross sectional design. The population was the housewives living in area III and V Bagan Deli, Medan Belawan sub-district for 631 persons, and the sample was taken for 87 persons. The data of the characteristics (education, income occupation, knowledge and attitude), management program, and the participation of the people was taken through direct interview using questionnaire. The collected data then analyzed using chi-square test at α = 0,05.The resulth of research showed that statistically the characteristics include education (p-value = 0,028), occupation status (p-value= 0,000). Knowledge level (p-value=0,002), and attitude (p-value=0,000) has significant relationship not have significant influence to the participation of the society. It si suggested for the management program parties to improve socialization about solid waste management program in rder to get better understanding on the rubbish and to improve the participation of the people

Design, analysis and investigation of an independent suspension for passenger cars

The objective of this paper is the design of a front suspension. The layout used is the McPherson strut, widely adopted for road cars due to its simplicity and to the limited space required. The handling, comfort and durability of the suspension are strictly related to the position of the hardpoints, and to the elastic elements. A sensitivity analysis is carried out to investigate the roll behavior of a standard vehicle during cornering. A multi-body dynamics software is used to perform ramp-steer simulations on a full-vehicle model. Results show the different peculiarities of three specific cases of analysis, each of them emphasising the effects of a specific parameter on the whole system

Localization length of a soliton from a non-magnetic impurity in a general double-spin-chain model

A localization length of a free-spin soliton from a non-magnetic impurity is deduced in a general double-spin-chain model ($J_0-J_1-J_2-J_3$ model). We have solved a variational problem which employs the nearest-neighbor singlet-dimer basis. The wave function of a soliton is expressed by the Airy function, and the localization length $(\xi)$ is found to obey a power law of the dimerization $(J_2-J_3)$ with an exponent -1/3; $\xi\sim (J_2-J_3)^{-1/3}$. This explains why NaV_2O_5 does not show the antiferromagnetic order, while CuGeO_3 does by impurity doping. When the gap exists by the bond-dimerization, a soliton is localized and no order is expected. Contrary, there is a possibility of the order when the gap is mainly due to frustration.Comment: 4 pages, REVTeX, Figures are in eps-file

Vehicle sideslip estimation for four-wheel-steering vehicles using a particle filter

The availability of the most relevant vehicle states is crucial for the development of advanced vehicle control systems and driver assistance systems. Specifically the vehicle sideslip angle plays a key role, yet this state is unpractical to measure and still not straightforward to estimate. This paper investigates a particle filter approach to estimate the chassis sideslip angle of road vehicles. The filter relies on a physical model of the vehicle and on measurements available from cheap and widespread sensors including inertial measurement unit and steering wheel angle sensor(s). The approach is validated using experimental data collected with the research platform RoboMobil (RoMo), a by-wire electric vehicle with wheel-individual traction and steering actuators. Results show that the performance of the proposed particle filter is satisfactory, and indicate directions for further improvement

IRF4 expression is low in Philadelphia negative myeloproliferative neoplasms and is associated with a worse prognosis

Interferon regulatory factor 4 (IRF4) is involved in the pathogenesis of various hematologic malignancies. Its expression has been related to the negative regulation of myeloid-derived suppressor cells (MDSCs) and the polarization of anti-inflammatory M2 macrophages, thereby altering immunosurveillance and inflammatory mechanisms. An abnormal inflammatory status in the bone marrow microenvironment of myeloproliferative neoplasms (MPNs) has recently been demonstrated; moreover, in chronic myeloid leukemia a downregulated expression of IRF4 has been found. In this context, we evaluated the IRF4 expression in 119 newly diagnosed consecutive Philadelphia negative MPNs (Ph- MPNs), showing a low expression among the MPNs phenotypes with a more significant decrease in primary myelofibrosis patients. Lower IRF4 levels were associated with JAK2 + and triple negatives cases carrying the worst prognosis. Furthermore, the IRF4 levels were related to leukemic transformation and a shorter leukemia-free survival; moreover, the risk of myelofibrosis transformation in polycythemia vera and essential thrombocythemia patients was more frequent in cases with lower IRF4 levels. Overall, our study demonstrates an IRF4 dysregulated expression in MPNs patients and its association with a worse prognosis. Further studies could validate these data, to improve our knowledge of the MPNs pathogenesis and confirm the IRF4 role as a new prognostic factor