Organic/inorganic hydrogels by simultaneous self-assembly and mineralization of aromatic short-peptides

This study was supported by projects RTI-2018-095794-A-C22, RYC-2016-21042 and project PID2020-118498GB-I00 funded by MCIN/AEI/10.13039/501100011033 and projects P18-FR-3533 and P18-TP-0969 by FEDER/Junta de Andalucia-Consejeria de Transformacion Economica, Industria, Conocimiento y Universidades (Spain). Thanks go to the CIC personnel of the University of Granada for technical assistance. MCMT acknowledges grant PRE2018-083773 funded by MCIN/AEI/10.13039/501100011033 and FSE "El FSE invierte en tu futuro", Spain. GBRR also acknowledges Junta de Andalucia for her postdoctoral contract within the PAIDI 2020 program ( DOC_01383).Self-assembled peptides and proteins have turned out to be excellent templates for the growth of inorganic minerals and can be used to emulate natural biomineralization processes. Doing this, researchers have developed complex sophisticated materials with properties, in some cases, similar to those found in nature. Of special interest is the development of scaffolds able to guide bone regeneration. The bone tissue comprises an organic matrix composed of aligned collagen fibers containing nanoapatite crystals oriented along the fiber direction. During bone mineralization, both processes, the self-assembly of collagen fibrils and mineralization occur simultaneously. Collagen fibers are able to control calcium phosphate nucleation and subsequent apatite crystal growth at a very limited range of collagen density and ionic concentration. In this study, we reproduced the simultaneity of both processes using an artificial peptide fluorenylmethoxycarbonyl-diphenylalanine (Fmoc-FF) that has the ability to self-assemble in water after the addition of Ca2+ ions. Therefore, the peptide self-assembly process and the mineralization of apatite are Ca-demanding processes and occur simultaneously. The role of peptide and ionic concentrations has been investigated affording organic/inorganic hybrid hydrogels with different degrees of homogeneity and mineralization. Interestingly, at very low Ca2+ concentrations, we found that apatite nanocrystals are integrated into Fmoc-FF fibrils and oriented as in biologically mineralized collagen fibrils, the basic building blocks of bone.FEDER/Junta de Andalucia-Consejeria de Transformacion Economica, Industria, Conocimiento y Universidades (Spain) P18-FR-3533 P18-TP-0969FSE "El FSE invierte en tu futuro", SpainJunta de Andalucia DOC_01383; RTI-2018-095794-A-C22; RYC-2016-21042; PID2020-118498GB-I00; MCIN/AEI/10.13039/501100011033; PRE2018-08377

Effectiveness of Following Mediterranean Diet Recommendations in the Real World in the Incidence of Gestational Diabetes Mellitus (GDM) and Adverse Maternal-Foetal Outcomes: A Prospective, Universal, Interventional Study with a Single Group. The St Carlos Study

We reported that a Mediterranean Diet (MedDiet), supplemented with extra-virgin olive oil (EVOO) and pistachios, reduces GDM incidence and several other adverse outcomes. In order to assess its translational effects in the real world we evaluated the effect of MedDiet from 1st gestational visit in GDM rate compared with control (CG) and intervention (IG) groups from the previously referred trial. As secondary objective we also compared adverse perinatal outcomes between normoglycemic and diabetic women. This trial is a prospective, clinic-based, interventional study with a single group. 1066 eligible normoglycaemic women before 12 gestational weeks were assessed. 932 women (32.4 ± 5.2 years old, pre-gestational BMI 22.5 ± 3.5 kg/m2) received a motivational lifestyle interview with emphasis on daily consumption of EVOO and nuts, were followed-up and analysed. Binary regression analyses were used to examine the risk for each pregnancy outcome, pregnancy-induced hypertension, preeclampsia, gestational weight gain (GWG), caesarean-section, perineal trauma, preterm delivery, small (SGA) and large for gestational age (LGA), and Neonatal Intensive Care Unit admissions. GDM was diagnosed in 13.9%. This rate was significantly lower than the CG: RR 0.81 (0.73–0.93), p < 0.001 and no different from the IG: RR 0.96 (0.85–1.07), p = 0.468. GWG was lower in diabetic women (10.88 ± 6.46 vs. 12.30 ± 5.42 Kg; p = 0.013). Excessive weight gain (EWG) was also lower in GDM [RR 0.91 (0.86–0.96); p < 0.001] without a significant increase of insufficient weight gain. LGA were also lower (1 (0.8%) vs. 31 (3.9%); p < 0.05)), and SGA were similar (5 (3.8%) vs. 30 (3.7%)). LGA were associated to EWG (RR 1.61 (1.35–1.91), p < 0.001). Differences in other maternal-foetal outcomes were not found. In conclusions an early MedDiet nutritional intervention reduces GDM incidence and maternal-foetal adverse outcomes and should be universally applied as 1st line therapy. GDM might not be consider as a high risk pregnancy any longer

Clonal chromosomal mosaicism and loss of chromosome Y in elderly men increase vulnerability for SARS-CoV-2

The pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, COVID-19) had an estimated overall case fatality ratio of 1.38% (pre-vaccination), being 53% higher in males and increasing exponentially with age. Among 9578 individuals diagnosed with COVID-19 in the SCOURGE study, we found 133 cases (1.42%) with detectable clonal mosaicism for chromosome alterations (mCA) and 226 males (5.08%) with acquired loss of chromosome Y (LOY). Individuals with clonal mosaic events (mCA and/or LOY) showed a 54% increase in the risk of COVID-19 lethality. LOY is associated with transcriptomic biomarkers of immune dysfunction, pro-coagulation activity and cardiovascular risk. Interferon-induced genes involved in the initial immune response to SARS-CoV-2 are also down-regulated in LOY. Thus, mCA and LOY underlie at least part of the sex-biased severity and mortality of COVID-19 in aging patients. Given its potential therapeutic and prognostic relevance, evaluation of clonal mosaicism should be implemented as biomarker of COVID-19 severity in elderly people. Among 9578 individuals diagnosed with COVID-19 in the SCOURGE study, individuals with clonal mosaic events (clonal mosaicism for chromosome alterations and/or loss of chromosome Y) showed an increased risk of COVID-19 lethality

Hybrid hydrogels by simultaneous self-assembly and mineralization of aromatic short-peptides

Resumen del póster presentado a la XXXVIII Reunión Bienal de la Real Sociedad Española de Química, celebrada en el Palacio de Congresos de Granada, del 27 de junio al 30 de junio de 2022.Bone is a complex tissue in continuous process of remodeling that maintains the structural integrity of the skeleton and protects inner organs. Bone pathologies (i.e., osteoporosis) are expected to increase due to the changes in population demographics. According to WHO, the worldwide hip fracture is expected to rise to 6 million by 2050. Despite the considerable progress in conventional scaffold-based tissue engineering, the recreation of physiological complexity has remained a challenge. Self-assembled peptides and proteins have turned out to be excellent templates for the growth of inorganic minerals trying to emulate natural biomineralization processes. Herein the biomimetic mineralization of amphiphilic short-peptides was studied with the final aim of designing sophisticated inorganic/organic nanocomposites with tunable structure-function relationships. Concretely, we have developed a protocol in which the self-assembly of amphiphilic peptides and the formation of apatite nanocrystals occurs simultaneously by addition of calcium ions in such a way that the competition for Ca2+ ions modulates crystal growth in a more precise way, emulating bone mineralization. As far as we know, both processes have never been studied simultaneously for this peptide. We found that substequiometric calcium concentration provides nanocomposite materials in which nanoapatite crystals are perfectly embedded and oriented along the peptide fibers (Figure1: Influence of Ca2+ concentration on the mineralization of aromatic peptides), as observed in mineralized collagen fibrils, the basic building blocks of the complex bone structure.Peer reviewe

Organic/inorganic hydrogels by simultaneous self-assembly and mineralization of aromatic short-peptides

Self-assembled peptides and proteins have turned out to be excellent templates for the growth of inorganic minerals and can be used to emulate natural biomineralization processes. Doing this, researchers have developed complex sophisticated materials with properties, in some cases, similar to those found in nature. Of special interest is the development of scaffolds able to guide bone regeneration. The bone tissue comprises an organic matrix composed of aligned collagen fibers containing nanoapatite crystals oriented along the fiber direction. During bone mineralization, both processes, the self-assembly of collagen fibrils and mineralization occur simultaneously. Collagen fibers are able to control calcium phosphate nucleation and subsequent apatite crystal growth at a very limited range of collagen density and ionic concentration. In this study, we reproduced the simultaneity of both processes using an artificial peptide fluorenylmethoxycarbonyl-diphenylalanine (Fmoc-FF) that has the ability to self-assemble in water after the addition of Ca2+ ions. Therefore, the peptide self-assembly process and the mineralization of apatite are Ca-demanding processes and occur simultaneously. The role of peptide and ionic concentrations has been investigated affording organic/inorganic hybrid hydrogels with different degrees of homogeneity and mineralization. Interestingly, at very low Ca2+ concentrations, we found that apatite nanocrystals are integrated into Fmoc-FF fibrils and oriented as in biologically mineralized collagen fibrils, the basic building blocks of bone.This study was supported by projects RTI-2018-095794-A-C22, RYC-2016-21042 and project PID2020-118498GB-I00 funded by MCIN/AEI/10.13039/501100011033 and projects P18-FR-3533 and P18-TP-0969 by FEDER/Junta de Andalucía-Consejería de Transformación Económica, Industria, Conocimiento y Universidades (Spain). Thanks go to the CIC personnel of the University of Granada for technical assistance. MCMT acknowledges grant PRE2018-083773 funded by MCIN/AEI/10.13039/501100011033 and FSE “El FSE invierte en tu futuro”, Spain. GBRR also acknowledges Junta de Andalucia for her postdoctoral contract within the PAIDI 2020 program (DOC_01383).Peer reviewe

Cáparra : revista de innovación y experiencias educativas de los Centros de Profesores y Recursos de la Provincia de Cáceres

Se describe un proyecto, realizado siguiendo la metodología de aprendizaje basado en proyectos del CEIP Reyes Católicos de Guadalupe (Cáceres) que consistió en la realización en soporte digital y en papel, en inglés y en español, de folletos informativos para dar a conocer distintas ermitas, granjas y molinos de la Edad Media, en el entorno de GuadalupeES

Salud y paisaje: contribución desde el termalismo a la revitalización de zonas rurales (el caso de Pozo Amargo, Cuenca Del Guadaira, España) Health and landscape: a contribution to the renew of rural lands (the case of Pozo Amargo, Cuenca Del Guadaira, España)

Ubicado en la contaminada cuenca del río Guadaíra (Andalucía, España) el balneario de Pozo Amargo es una buena muestra del rico patrimonio arquitectónico vinculado al agua que han generado sus habitantes a lo largo de estos últimos siglos. Este patrimonio ha experimentado un abandono similar al del propio río pero tanto el movimiento ciudadano como la respuesta de la administración han permitido paliar ambas situaciones. Hoy en día, la rehabilitación del antiguo balneario puede suponer una interesante iniciativa que aúne la mejora ambiental y paisajística del alto Guadaíra con la recuperación del patrimonio artístico y etnográfico de la zona, así como la puesta en marcha de una experiencia pionera de proyecto paisajístico aplicado a destinos turísticos de salud capaz de generar riqueza en la zona.<br>Located in the polluted basin of Guadaira's river (Andalusia, Spain), the Pozo Amargo's spa is a good example of a rich architectonical heritage related to water that inhabitants have created over the last few centuries. This heritage has suffered a similar degradation to the river, but both the citizen's movement and the Administration's response have made possible the mitigation of both situations. Nowadays, the restoration of the former spa can be an exciting initiative that joins the environmental improvements and the landscape value of the Alto Guadaira's with the recovery of artistic and ethnographic heritage of this area. Moreover, it will make possible the launching of a pioneering experience of a landscape's project applied to touristic and health destinations that can generate wealth in the area

Autorité et pouvoir dans le théâtre du Siècle d'Or

On connaît l’interprétation défendue par José Antonio Maravall, qui voyait dans le théâtre du Siècle d’Or une machine de propagande du pouvoir. Les choses ne peuvent pas être aussi simples dans une société et une culture aussi complexes et qui ont vu naître des dramaturges tels que Lope ou Calderón et des narrateurs comme Cervantès. Que le théâtre du Siècle d’Or ait défendu le système ne peut se dire sans en nuancer divers aspects. Le présent volume du Bulletin Hispanique aborde certains de ces problèmes appliqués à une large liste d’auteurs. Au premier plan, Tirso de Molina et Calderón, mais aussi des auteurs et des pièces moins connus : le criollo Peralta Barnuevo, la Epístola de Amarilis a Belardo, Enríquez Gómez, Pérez de Montalbán ou Lope de Liaño. Les points de vue sont tout aussi vastes et variés : depuis l’annotation philologique jusqu’aux thématiques morales, mythiques et anthropologiques ; les genres et conventions ; les problèmes de traduction et de transposition à d’autres contextes culturels ; les figures incarnant différents modes de pouvoir ; la survivance ou la caducité des modèles ; la relation théorie/pratique ; sans oublier la question classique de l’inconstante fortune des favoris ou ce symbole central des emblèmes politiques : le cheval. Es conocida la interpretación que defendió José Antonio Maravall, quien veía en el teatro del Siglo de Oro una máquina de propaganda del poder. Las cosas no son tan simples. No lo pueden ser en una sociedad y una cultura tan compleja en la que florecieron dramaturgos como Lope y Calderón, o narradores como el mismo Cervantes. Que el teatro del Siglo de Oro defienda el sistema no se puede decir sin matizar múltiples aspectos. El presente volumen del Bulletin hispanique aborda algunos de estos problemas. Es de subrayar la amplitud del arco contemplado: destacan las figuras de Tirso de Molina y de Calderón pero no faltan análisis sobre autores y piezas menos estudiadas : el criollo Peralta Barnuevo, la Epístola de Amarilis a Belardo, Enríquez Gómez, Pérez de Montalbán o Lope de Liaño. Igualmente amplios y diversos son los enfoques: desde la anotación filológica a las temáticas, morales, míticas y antropológicas; cuestiones genéricas y de convenciones; problemas de traducción y trasposición a otros ambientes culturales; figuras que encarnan distintos modos de poder; pervivencia o caducidad de modelos; relación teoría/práctica; sin olvidar un asunto tan clásico como la voltaria fortuna de los privados o un símbolo nuclear de la emblemática política como el del caballo. One knows the interpretation championed by José Antonio Maravall, who saw in the theatre of the Golden Century, a propaganda machine for the power. Things cannot be that simple in such a complex society and culture, which saw the birth of playwrights like Lope or Calderón, or writers like Cervantès. That the theatre had defended the political system in the Golden Century cannot be said without nuances. The present volume of the Bulletin Hispanique tackles with some of these problems, applied to a large list of authors. Tirso de Molina and Calderón in the first place, but also authors of less known plays: the criollo Peralta Barnuevo, the “Epístola de Amarilisa Belardo”, Enríquez Gómez, Pérez de Montalbán ou Lope de Liaño. The points of view are as vast and varied: from philological annotation to moral, mythological and anthropological themes; genres and conventions; problems of translation and transposition to other cultural contexts; the figures embodying diverse modes of power; the survival or nullity of models; the relation theory/practice; without forgetting the usual question of the inconstant good fortune of favourites, or the symbol, central in political emblems: the horse

Autorité et pouvoir dans le théâtre du Siècle d'Or

On connaît l’interprétation défendue par José Antonio Maravall, qui voyait dans le théâtre du Siècle d’Or une machine de propagande du pouvoir. Les choses ne peuvent pas être aussi simples dans une société et une culture aussi complexes et qui ont vu naître des dramaturges tels que Lope ou Calderón et des narrateurs comme Cervantès. Que le théâtre du Siècle d’Or ait défendu le système ne peut se dire sans en nuancer divers aspects. Le présent volume du Bulletin Hispanique aborde certains de ces problèmes appliqués à une large liste d’auteurs. Au premier plan, Tirso de Molina et Calderón, mais aussi des auteurs et des pièces moins connus : le criollo Peralta Barnuevo, la Epístola de Amarilis a Belardo, Enríquez Gómez, Pérez de Montalbán ou Lope de Liaño. Les points de vue sont tout aussi vastes et variés : depuis l’annotation philologique jusqu’aux thématiques morales, mythiques et anthropologiques ; les genres et conventions ; les problèmes de traduction et de transposition à d’autres contextes culturels ; les figures incarnant différents modes de pouvoir ; la survivance ou la caducité des modèles ; la relation théorie/pratique ; sans oublier la question classique de l’inconstante fortune des favoris ou ce symbole central des emblèmes politiques : le cheval. Es conocida la interpretación que defendió José Antonio Maravall, quien veía en el teatro del Siglo de Oro una máquina de propaganda del poder. Las cosas no son tan simples. No lo pueden ser en una sociedad y una cultura tan compleja en la que florecieron dramaturgos como Lope y Calderón, o narradores como el mismo Cervantes. Que el teatro del Siglo de Oro defienda el sistema no se puede decir sin matizar múltiples aspectos. El presente volumen del Bulletin hispanique aborda algunos de estos problemas. Es de subrayar la amplitud del arco contemplado: destacan las figuras de Tirso de Molina y de Calderón pero no faltan análisis sobre autores y piezas menos estudiadas : el criollo Peralta Barnuevo, la Epístola de Amarilis a Belardo, Enríquez Gómez, Pérez de Montalbán o Lope de Liaño. Igualmente amplios y diversos son los enfoques: desde la anotación filológica a las temáticas, morales, míticas y antropológicas; cuestiones genéricas y de convenciones; problemas de traducción y trasposición a otros ambientes culturales; figuras que encarnan distintos modos de poder; pervivencia o caducidad de modelos; relación teoría/práctica; sin olvidar un asunto tan clásico como la voltaria fortuna de los privados o un símbolo nuclear de la emblemática política como el del caballo. One knows the interpretation championed by José Antonio Maravall, who saw in the theatre of the Golden Century, a propaganda machine for the power. Things cannot be that simple in such a complex society and culture, which saw the birth of playwrights like Lope or Calderón, or writers like Cervantès. That the theatre had defended the political system in the Golden Century cannot be said without nuances. The present volume of the Bulletin Hispanique tackles with some of these problems, applied to a large list of authors. Tirso de Molina and Calderón in the first place, but also authors of less known plays: the criollo Peralta Barnuevo, the “Epístola de Amarilisa Belardo”, Enríquez Gómez, Pérez de Montalbán ou Lope de Liaño. The points of view are as vast and varied: from philological annotation to moral, mythological and anthropological themes; genres and conventions; problems of translation and transposition to other cultural contexts; the figures embodying diverse modes of power; the survival or nullity of models; the relation theory/practice; without forgetting the usual question of the inconstant good fortune of favourites, or the symbol, central in political emblems: the horse

Guía de diseño y mejora continua de procesos asistenciales integrados. 2ª ed

Publicado en la página web de la Consejería de Igualdad, Salud y Políticas Sociales: www.juntadeandalucia.es/salud (Consejería de Salud / Profesionales / Nuestro Compromiso por la Calidad / Procesos Asistenciales Integrados)YesEsta segunda edición pretende potenciar e impulsar los principios básicos del abordaje de los Procesos Asistenciales Integrados (PAI) en Andalucía y establecer la metodología para la actualización y descripción de los PAI para este nuevo impulso