Gas exchange and water relations of young apricot plants under drought conditions

Potted 1.5-year-old apricot plants (Prunus armeniaca L.), growing under polycarbonate glasshouse conditions with a cooling system, were subjected to two successive water stress/recovery periods until pre-dawn leaf water potential (Ypd) reached values between -2.0 and -2.5 MPa, during summer 1996. Control plants were irrigated daily to maintain the soil matric potential at c. -20 kPa. Water stress limited plant growth and induced a significant reduction in leaf area, caused by mature leaf abscission. The parallel behaviour of leaf turgor potential and epinasty in stressed plants indicated that these movements are turgor-dependent. Osmotic adjustments of 0.27 and 0.60 MPa were observed at the end of the first and second stress period, respectively. Relative apoplastic water content (RWCa) values were high, ranging from 27 to 42%, and were not affected by water stress. The rapid decrease in leaf conductance (gl) from the beginning of the stress periods, together with the delay in stomatal reopening after rewatering the plants, indicated that stomatal behaviour was not a simple passive response to water deficits. Net photosynthesis decreased only at the end of both stress periods and recovered quickly. These observations indicate that leaf productivity may be affected only slightly by short-term water stress. The results indicate that drought resistance in apricot is based mainly on avoidance mechanisms, such as stomatal control, epinasty and limitation of transpiration by reducing leaf area. However, some tolerance characteristics, including osmotic adjustment, high RWCa and low leaf osmotic potential at turgor loss point (Ytlp) values were observed.The authors are grateful to J. Soto-Montesinos, M.D. Velasco, M. García and E. Nicolás for their assistance. The study was supported by CICYT (AMB95- 0071) and Consejería de Medio Ambiente, Agricultura y Agua de Murcia grants to the authors

Assessment of discretely measured indicators and maximum daily trunk shrinkage for detecting water stress in pomegranate trees

8 páginas.-- 7 figuras.-- 3 tablas.-- 50 referenciasMeasurements obtained by the continuous monitoring of trunk diameter fluctuations were comparedwith discrete measurements of midday stem water potential and midday leaf conductance (gl)in adult pomegranate trees (Punica granatum L. cv. Mollar de Elche). Control plants (T0) were irrigateddaily above their crop water requirements in order to attain non-limiting soil water conditions in 2009 and 2010, while T1 plants were subjected to water stress by depriving them of irrigation water for34 days in 2010, after which time irrigation was restored and plant recovery was studied for 7 days.T1 plants showed a substantial degree of water stress, which developed slowly. Maximum daily trunkshrinkage (MDS) was identified to be the most suitable plant-based indicator for irrigation scheduling inadult pomegranate trees, because its signal:noise ((T1/T0):coefficient of variation) ratio was higher thanthat for midday stem water potential((T1/T0):coefficient of variation) and gl((T0/T1):coefficient of variation). MDS increasedin response to water stress, but when the stemfell below ¿1.67 MPa, the MDS values decreased. Fornon-limiting water conditions, MDS could be predicted from mean daily air temperature (Tm) throughexponential equations fitted to pooled data across several seasons. First-order equations were alsoobtained by pooling data across several seasons to predict MDS from crop reference evapotranspira-tion (ETo), mean daily air vapour pressure deficit (VPDm), Tmand solar radiation (Rs), but these should beused only within a certain range of values (ETo, 2.1¿7.4 mm; VPDm, 0.64¿2.96 kPa; Tm, 12.1¿28.3¿C; Rs,119.4¿331.3 Wm¿2). Hence, automated MDS measurements have the potential to be used in irrigationscheduling of pomegranate, and these values can be normalized to non-limiting water conditions bylocally derived empirical relationships with meteorological variables.This research was supported by Ministerio de Ciencia e Innovación (MICINN) (CICYT/FEDER AGL2010-19201-C04-01AGR) and Agencia Española de Cooperación Internacional para el Desarrollo (AECID) (A1/035430/11) grants to the authors

Regulated deficit irrigation in table olive trees during a sensitive period

Olive tree is one of the most important irrigated fruit at Spain (around 400.000 ha). The water needs in olive orchard are greater than the water availability. Therefore, deficit conditions are common at the field. The aim of this work is to study a regulated deficit irrigation (RDI) scheduling based on midday stem water potential (Y) that limits irrigation before harvest. The experiment was performed at La Hampa experimental farm (Coria del río, Seville, Spain) in 45 years-old olive (cv Manzanillo). Three irrigation treatments in a complete randomized block design were performed during 2014.This research was supported by the Spanish Ministerio de Economía y Competitividad (MINECO), (AGL2013-45922-C2-1-R).Peer Reviewe

Limitations and usefulness of maximum daily shrinkage (MDS) and trunk growth rate (TGR) indicators in the irrigation scheduling of table olive trees

8 páginas.-- 7 figuras.-- 2 tablas.-- 32 referenciasMaximum daily trunk shrinkage (MDS) is the most popular indicator derived from trunk diameter fluctuations in most fruit trees and has been reported to be one of the earliest signs in the detection of water stress. However, in some species such as olive trees (. Olea europaea L.), MDS does not usually change in water stress conditions and trunk growth rate (TGR) has been suggested as better indicator. Most of this lack of sensitivity to drought conditions has been related to the relationship between the MDS and the water potential. This curvilinear relationship produces an uncertain zone were great variations of water potential do not imply any changes of MDS. The MDS signal, the ratio between measured MDS and estimated MDS with full irrigation, has been thought to be a better indicator than MDS, as it reduces the effect of the environment. On the other hand, though literature results suggest an effect of environment in TGR values, there are not clear relationship between this indicator and meteorological data. The aims of this work are, on one hand, to study the improvements of the baseline approach in the MDS signal and, on the other, study the influence of several meteorological variables in TGR. Three years' data from an irrigation experiment were used in to carry out the MDS analysis and six years' data for full irrigated trees during pit hardening period were used for TGR study. The comparison between MDS vs. water potential and MDS signal vs. water potential presented a great scattering in both relationships. Values of MDS signal between 1.1 and 1.4 were always identified with moderate water stress conditions (-1.4 to -2. MPa of water potential). However, since this MDS signal values are around the maximum in the curvilineal relationship with water potential, greater values of MDS signal (in the range of 1.1-1.4) were not necessary lower values of water potential. In addition, during low fruit load seasons MDS signal was not an accurate indicator. On the other hand, absolute values of several climatological measurements were not significantly related with TGR. Only daily increments explain part of the variations of TGR in full irrigated trees. In all the data analysed, the daily increment of average vapour pressure deficit was the best indicator related with TGR. The increase of this indicator decreased TGR values. In addition, the agreement between this indicator and TGR was affected for fruit load. Great yield seasons decrease the influence of VPD increment in TGR.This research was supported by the Spanish Ministerio de Ciencia e Innovación (MICINN), (AGL2010-19201-CO4-03). Thanks are due to J. Rodriguez and A. Montero for help with field measurements.Peer reviewe

Seasonal changes of maximum daily shrinkage reference equations for irrigation scheduling in olive trees: influence of fruit load

Maximum daily shrinkage (MDS) is the parameter of daily cycle of trunk diameter most widely suggested in irrigation scheduling for several fruit trees. However, as in other plant-measured approaches, the irrigation decision may be difficult due to the influence of the environment on the values obtained. Reference equations of MDS have been established in order to avoid the effects of environmental conditions. Such equations are usually related to simple meteorological data, in order to easily estimate MDS values in full-irrigated conditions. This paper studies the influence of fruit load and the inter-annual variations on the reference equation of MDS in olive trees. These reference equations were calculated during 4 seasons in a full-irrigated orchard and the equations were validated with the data from a different season. The MDS values were related to vapour pressure deficit (VPD) and temperature taken near the experimental orchard. In addition, meteorological data were considered as mean daily or as midday values: only for temperature was the maximum daily value also used. The validation of the equations was made using the fits with all the meteorological data considered (midday and mean daily of VPD and temperature). In addition, two different fits were used in each meteorological data one according to fruit load and other with the complete pool of data. The equations fit were significantly different each season in all the meteorological data considered. However, seasons with similar fruit load were more similar to each other. In both meteorological data considered (VPD and temperature) the midday values improved the fit in respect to mean daily values. The equations obtained with maximum daily temperature were similar in accuracy to the one of midday. The reference equations in which temperature was used obtained a better fit that the ones calculated with VPD. No significant differences were found in the validation when equations according to fruit load or the complete pool data were compared. The limitations and usefulness of these reference equations are also discussed.Ministerio de Ciencia e Innovación, Unión Europea AGL2004-0794-C03-02 and AGL2007-66279-C03-02/AG

Pattern of trunk diameter fluctuations of almond trees in deficit irrigation scheduling during the first seasons

Irrigation needs in mature almond orchards are very high. Although almond trees grow in rainfed conditions, the yield response is very sensitive to irrigation. Continuous monitoring of the water status could be an adequate tool to optimize deficit irrigation. In this sense, trunk diameter fluctuations appeared as a very promising indicator at the beginning of the century, but few data have been published. The aim of this work is to check threshold values of maximum daily shrikage (MDS) and identify possible limitations to their use in commercial orchards. The experiment was performed in a commercial farm in Dos Hermanas (Seville, Spain) during the 2017 season on a 7-years-old orchard (cv Vairo). The irrigation treatments were Control (100% ETc), sustained deficit irrigation (SDI) with a maximum seasonal irrigation of 100 mm and two regulated deficit treatments (RDI). Both RDI treatments (RDI-1 and RDI-2) were scheduled using the signal of maximum daily shrinkage (signal) and the midday stem water potential (SWP). In RDI-1, full irrigation conditions were provided before kernel filling and during postharvest, using the threshold values suggested in the bibliography. During kernel filling, the water stress level was designed to be -1.5 MPa (SWP) and 1.75 (signal). RDI-2 trees were irrigated using the same scheduling as RDI-1, but target water stress values were higher in kernel filling (-2 MPa and 2.75) and with a maximum seasonal amount of water of 100 mm. SWP in Control trees was near the McCutchan and Shackel baseline for most of the season. None of the deficit treatments reached the signal values suggested. Moreover, the signal values were almost equal between treatments, with no water stress effect. The trunk growth rate (TGR) presented clear differences depending on the water status

Programación del riego deficitario controlado en aceituna de mesa empleando la dendrometría

La agricultura de riego es actualmente el mayor consumidor de agua en el mundo (Fereres y Evans 2006)). En muchas zonas áridas y semiáridas la escasez de recursos hídricos junto con la creciente demanda de agua para otros usos están imponiendo una fuerte presión para limitar su disponibilidad en la agricultura, un ejemplo muy claro es el caso de la agricultura Mediterránea. La optimización de este recurso natural se puede obtener a varios niveles pero posiblemente el menos estudiado y, con seguridad, el menos empleado a nivel comercial sea la programación del riego deficitario. Todavía es escasa la implantación de técnicas que realicen una toma de decisiones de riego de forma continua y telemática. El olivar, como cultivo tradicional y, muy recientemente, de regadío es un buen ejemplo

Fracciones de hierro y metaloenzimas en el estudio de la clorosis férrica en Citrus

Tesis Doctoral inédita leída en la Universidad Autónoma de Madrid. Facultad de Ciencias, Departamento de Química Agrícola. Fecha de lectura 16-05-197

For a better use and distribution of water: An introduction

Many agricultural areas in the Iberian Peninsula are characterized by the aridity of the climate and the persistent shortage and low quality of the available water resources. In the last 30 years 17% of the European territory has had drought periods (EC,2007),which affected 11% of the population and caused losses of D100 billion (Collins et al.,2009). Spain is the most arid country in the European Union (EU), not only because of its average precipitation, which amounts to ca. 85% of that in the EU, but because of its great atmospheric demand (López et al.,2008). The scarce water resources are increasingly disputed among different productive sectors because of the expansion of urban, touristic and industrial activities. We should not be surprised, therefore, on the growing pressure for a more sustainable use of water in agriculture. The fact that growers in arid and semi-arid areas of Spain and Portugal,and of many other Mediterranean agrosystems, are bound to face water scarcity has long been known (Pereira et al., 2002). But we must get ready for more severe water restrictions, since the frequency and severity of drought events driven by climate change are expected to increase (Houghtonet al.,1995; Collins et al.,2009). To cope with this situation, new water management policies are being developed, both at the European and national levels. This is the case of the EU Water Framework Directive (WFD, Directive 2000/60/EC), the program AGUA or Actions for the Management and Use of Water (Spanish Ministry of Environment, 2004), and the Portuguese update of the previous Plano Nacional da Água (Ministerio do Ambiente e do Ordenamento do Território, 2002).Peer Reviewe