A Design-Led, Materials Based Approach to Human Centered Applications Using Modified Dielectric Electroactive Polymer Sensors

This paper describes a design-led exploratory scoping study into the potential use of an industry standard dielectric electroactive polymer (DEAP) sensor for applications in assistive healthcare. The focus of this activity was to explore the physical format and integration of soft materials and sensor combinations with properties that afford an opportunity for accurate and unobtrusive real time body mapping and monitoring. The work involved a series of practical investigations into the capacitance changes in the sensor brought on by deformation through different ways of stretching. The dielectric sensors were selected as a direct mapping tool against the body based on the similarity of the stretch qualities of both the sensor and human skin and muscle resulting in a prototype vest for real time breathing monitoring through sensing thoracic movement. This involved modification of the standard sensors and handcrafting bespoke sensors to map critically relevant areas of the thorax

Summary Report on 2022 Residue Monitoring of Irish Farmed Fish & 2022 Border Inspection Post Fishery Product Testing of Samples Received into the Marine Institute

On behalf of the Department of Agriculture, Food and Marine (DAFM), the Marine Institute carries out monitoring of chemical residues in finfish for aquaculture sector. This monitoring is set out in the annual National Residue Control Plan, which is approved by the European Commission, and is an important component of the DAFM food safety controls and is implemented under a service contract with the Food Safety Authority of Ireland. Since 1999, the Marine Institute has implemented the National Residues Monitoring Programme for aquaculture. This is carried out on behalf of the Sea Fisheries Protection Authority, which is the responsible organisation for residue controls on farmed finfish. In 2022, in excess of 803 tests and a total of 8,263 measurements were carried out on 138 samples of farmed finfish for a range of residues. Implementation of the Aquaculture 2022 Plan involves taking samples at both farm and processing plant: 92 target samples taken at harvest: 82 farmed salmon and 10 freshwater trout. 46 target samples were taken at other stages of production: 36s salmon smolts and 10 freshwater trout. All 2022 samples were compliant. For target sampling of farmed fish, a summary table of the residue results from 2005 - 2022 is outlined in Table 1. Overall, the outcome for aquaculture remains one of consistently low occurrence of residues in farmed finfish, with no non-compliant target residues results for the period 2006-2014, 0.11% and 0.10% non-compliant target residues results in 2015 and 2016 respectively and no non-compliant target results for the period 2017 to 2022.Marine Institut

The Power of ‘Soft’

Over the last decade, the explosion in research and Development associated with nanoscalar materials has continued apace. In parallel with this has been the rapid rise of both sustainable materials and, as a consequence, Natural, Cellular and Responsive material systems. Many of these originate from inorganic, inorganic-organic hybrid composites and polymeric and bio-nano polymeric systems which exhibit intrinsic physico-chemical properties that can be classed as ‘soft’. That is flexible, malleable, lightweight, transparent or semi-transparent and stretchable in character and which can also offer both biocompatible and bioresorbable characteristics essential to useable and sustainable material systems. This paper describes some of the ways in which we are beginning to understand, explain and exploit ‘soft’ technology. In particular the interactive role of creative design and innovative material science linked through new fabrication methodologies that have, as their common purpose, a focus on compelling Human centred needs. Examples are health, wellness, ambient assistance and urgent improvements in cleanliness, hygiene and nutrition

Transfer of Antibiotic Resistance Marker Genes between Lactic Acid Bacteria in Model Rumen and Plant Environments▿

Three wild-type dairy isolates of lactic acid bacteria (LAB) and one Lactococcus lactis control strain were analyzed for their ability to transfer antibiotic resistance determinants (plasmid or transposon located) to two LAB recipients using both in vitro methods and in vivo models. In vitro transfer experiments were carried out with the donors and recipients using the filter mating method. In vivo mating examined transfer in two natural environments, a rumen model and an alfalfa sprout model. All transconjugants were confirmed by Etest, PCR, pulsed-field gel electrophoresis, and Southern blotting. The in vitro filter mating method demonstrated high transfer frequencies between all LAB pairs, ranging from 1.8 × 10−5 to 2.2 × 10−2 transconjugants per recipient. Transconjugants were detected in the rumen model for all mating pairs tested; however, the frequencies of transfer were low and inconsistent over 48 h (ranging from 1.0 × 10−9 to 8.0 × 10−6 transconjugants per recipient). The plant model provided an environment that appeared to promote comparatively higher transfer frequencies between all LAB pairs tested over the 9-day period (transfer frequencies ranged from 4.7 × 10−4 to 3.9 × 10−1 transconjugants per recipient). In our test models, dairy cultures of LAB can act as a source of mobile genetic elements encoding antibiotic resistance that can spread to other LAB. This observation could have food safety and public health implications

Epidemiology of undiagnosed depression in people with diabetes mellitus: a comparative analysis of Ireland, England and the USA

Objectives Improving detection of depression in people with diabetes is recommended. However, little is known about how different health systems compare in depression detection. We estimated and compared the (1) prevalence of depression detection in people with and without diabetes, and (2) association between diabetes and undiagnosed depression across three health systems. Design Cross-sectional analysis of three nationally representative studies: The Irish Longitudinal Study on Ageing, the English Longitudinal Study on Ageing and the Health and Retirement Study. Setting Community-dwelling adults in Ireland, England and the USA

Developing a core outcome set for childhood obesity prevention: a systematic review

Synthesis of effects of infant feeding interventions to prevent childhood obesity is limited by outcome measurement and reporting heterogeneity. Core outcome sets (COSs) represent standardised approaches to outcome selection and reporting. The aim of this review is to identify feeding outcomes used in infant feeding studies to inform an infant feeding COS for obesity prevention interventions. The databases EMBASE, Medline, CINAHL, CENTRAL, and PsycINFO searched from inception to February 2017. Studies eligible for inclusion must examine any infant feeding outcome in children ≤1 year. Feeding outcomes include those measured using self‐report and/or observational methods and include dietary intake, parent–child interaction, and parental beliefs, among others. Data were extracted using a standardised data extraction form. Outcomes were assigned to outcome domains using an inductive, iterative process with a multidisciplinary team. We identified 82 unique outcomes, representing nine outcome domains. Outcome domains were “breast and formula feeding,” “introduction of solids,” “parent feeding practices and styles,” “parent knowledge and beliefs,” “practical feeding,” “food environment,” “dietary intake,” “perceptions of infant behaviour and preferences,” and “child weight outcomes.” Heterogeneity in definition and frequency of outcomes was noted in reviewed studies. “Introduction of solids” (59.5%) and “breastfeeding duration” (55.5%) were the most frequently reported outcomes. Infant feeding studies focus predominantly on consumption of milks and solids and infant weight. Less focus is given to modifiable parental and environmental factors. An infant feeding COS can minimise heterogeneity in selection and reporting of infant feeding outcomes for childhood obesity prevention interventions.ACCEPTEDpeer-reviewe

Development of an infant feeding core outcome set for childhood obesity interventions: study protocol

Background: Childhood obesity is a significant public health challenge that affects approximately one in five children worldwide. Infant feeding practices are implicated in the aetiology of childhood obesity. Infant feeding interventions for childhood obesity are increasingly popular but outcome reporting is inconsistent across trials. Lack of standardisation limits examination of intervention effects and mechanisms of change. The aim of the current project is to develop a core set of infant feeding outcomes for children <= 1 year old, to be evaluated in childhood obesity intervention trials. Methods: This project will use similar methodology to previous core outcome development research. An infant feeding core outcome set (COS) will be developed in four stages: (1) a systematic review of the literature, (2) discussion and clarification of outcomes in a meeting involving multiple stakeholder perspectives, (3) prioritisation of outcomes using the Delphi technique with an expert panel of stakeholders, and (4) achieving consensus on the COS using the nominal group technique (NGT) consensus meeting. An online Delphi survey will be conducted following the NGT meeting to prioritise outcomes identified in the systematic review. An NGT meeting will be conducted with groups of health professionals, non-clinician researchers, and parents of infants <= 1 year old, to achieve final consensus on the infant feeding COS. Discussion: This study aims to develop a core outcome set of infant feeding outcomes for randomised infant feeding studies to prevent childhood obesity. This research will improve examination and syntheses of the outcomes of such studies to prevent and reduce childhood obesity

Development of an infant feeding core outcome set for childhood obesity interventions: study protocol

Background: Childhood obesity is a significant public health challenge that affects approximately one in five children worldwide. Infant feeding practices are implicated in the aetiology of childhood obesity. Infant feeding interventions for childhood obesity are increasingly popular but outcome reporting is inconsistent across trials. Lack of standardisation limits examination of intervention effects and mechanisms of change. The aim of the current project is to develop a core set of infant feeding outcomes for children <= 1 year old, to be evaluated in childhood obesity intervention trials. Methods: This project will use similar methodology to previous core outcome development research. An infant feeding core outcome set (COS) will be developed in four stages: (1) a systematic review of the literature, (2) discussion and clarification of outcomes in a meeting involving multiple stakeholder perspectives, (3) prioritisation of outcomes using the Delphi technique with an expert panel of stakeholders, and (4) achieving consensus on the COS using the nominal group technique (NGT) consensus meeting. An online Delphi survey will be conducted following the NGT meeting to prioritise outcomes identified in the systematic review. An NGT meeting will be conducted with groups of health professionals, non-clinician researchers, and parents of infants <= 1 year old, to achieve final consensus on the infant feeding COS. Discussion: This study aims to develop a core outcome set of infant feeding outcomes for randomised infant feeding studies to prevent childhood obesity. This research will improve examination and syntheses of the outcomes of such studies to prevent and reduce childhood obesity

A standardized conjugation protocol to asses antibiotic resistance transfer between lactococcal species

Optimal conditions and a standardized method for conjugation between two model lactococcal strains, Lactococcus lactis SH4174 (pAMbeta1-containing, erythromycin resistant donor) and L. lactis Bu2-60 (plasmid-free, erythromycin sensitive recipient), were developed and tested in a inter-laboratory experiments involving five laboratories from different countries. The ultimate goal of the study was to assess the microbial potential of antibiotic resistance transfer among Lactic Acid Bacteria (LAB). The influence of culture age (various OD values) and ratios of donor and recipient cultures as well as filter, solid and liquid mating techniques, were examined in order to optimize the conjugation protocol. In the result of these studies, we concluded that the donor-to-recipient ratio appear to be important; the most efficient technique for conjugation was filter mating and the optimal conditions for gene transfer were observed when late logarithmic cultures of both donor and recipient were used. Comparison of conjugal transfer frequencies between five partner laboratories showed that results are sufficiently intra-laboratory repeatable and inter-laboratory comparable. This is the first study of this kind, in which a standardized protocol of conjugal mating for testing antibiotic resistance dissemination among LAB was established and validate