Prevalence of major foliar and panicle diseases of Sorghum (Sorghum bicolor [L.] Moench) in the Deccan plateau of India

Extensive on-farm disease surveys were conducted from August 1999 until March 2001 in four sorghum-growing states of the Indian Deccan plateau. A total of 965 fields were surveyed covering 228 fields in Andhra Pradesh (AP), 406 in Karnataka (KAR), 290 in Maharashtra (MH) and 41 in Tamil Nadu (TN). Among 14 foliar diseases observed, maize stripe virus (MStV), a tenuivirus transmitted by the delphacid plant hopper (Peregrinus maidis), and among five panicle diseases, ergot or sugary disease (Claviceps sorghi and C. africana) were the most destructive diseases. MStV was prevalent in 28.4% and ergot in 13.4% of the fields surveyed in two years across four states. Yet, the mean incidence of MStV in AP was 6% with 85% mean severity. The values in KAR were 12% incidence and 83% severity, in MH 5% and 67%, and in TN 12% and 76%, respectively. The mean incidence of ergot in AP was 34% with 67% mean severity. The values in KAR were 41% and 79%, in MH, 30% and 67%, and in TN 100% and 100%, respectively. Variation in frequency of occurrence of MStV was observed between 1999 and 2001. Variations in frequency could be due to weather factors, vector survival, cropping pattern, and host specificity. The frequency of ergot also was varying among years, locations, seasons and cultivars. An ergot epidemic was observed during the 1999 rainy season in Maachinenipalli village (16°35′N; 78°3′E), Andhra Pradesh. In September 2000, the disease had spread to 13 neighboring administrative zones damaging about 130 000 ha. This paper elucidates the distribution of diseases observed between 1999 and 2001 but does not imply that the diseases are restricted necessarily to a particular zone or location

Cerebral arteriopathy associated with heterozygous variants in the casitas B-lineage lymphoma gene

Objective: To report a series of patients with cerebral arteriopathy associated with heterozygous variants in the casitas B-lineage lymphoma (CBL) gene and examine the functional role of the identified mutant Cbl protein. We hypothesized that mutated Cbl fails to act as a negative regulator of the RAS-mitogen-activated protein kinases (MAPK) signaling pathway, resulting in enhanced vascular fibroblast proliferation and migration and enhanced angiogenesis and collateral vessel formation. Methods: We performed whole-exome sequencing in 11 separate families referred to Great Ormond Street Hospital, London, with suspected genetic cause for clinical presentation with severe progressive cerebral arteriopathy. Results: We identified heterozygous variants in the CBL gene in 5 affected cases from 3 families. We show that impaired CBL-mediated degradation of cell surface tyrosine kinase receptors and dysregulated intracellular signaling through the RAS-MAPK pathway contribute to the pathogenesis of the observed arteriopathy. Mutated CBL failed to control the angiogenic signal relay of vascular endothelial growth factor receptor 2, leading to prolonged tyrosine kinase signaling, thus driving angiogenesis and collateral vessel formation. Mutant Cbl promoted myofibroblast migration and proliferation contributing to vascular occlusive disease; these effects were abrogated following treatment with a RAF-RAS-MAPK pathway inhibitor. Conclusions: We provide a possible mechanism for the arteriopathy associated with heterozygous CBL variants. Identification of the key role for the RAS-MAPK pathway in CBL-mediated cerebral arteriopathy could facilitate identification of novel or repurposed druggable targets for treating these patients and may also provide therapeutic clues for other cerebral arteriopathies.This article is freely available via Open Access. Click on the Publisher URL to access it via the publisher's site.Y. Hong was supported for this work by Versus Arthritis (grant 21791) and Rosetrees Trust (grant A1700). D. Eleftheriou was supported by Versus Arthritis (grants 20164 and 21593). A. Keylock was supported by a BHF PhD studentship. B. Jensen is supported by a GOSH Children’s Charity grant (CP_RSRCH_003). P.A. Brogan and D. Eleftheriou also acknowledge support from Great Ormond Street Hospital Children’s Charity. All research at Great Ormond Street Hospital NHS Foundation Trust and UCL Great Ormond Street Institute of Child Health is made possible by the NIHR Great Ormond Street Hospital Biomedical Research Centre. The views expressed are those of the author(s) and not necessarily those of the NHS, the NIHR, or the Department of Health.accepted version (12 month embargo

Determining crystal structures through crowdsourcing and coursework

We show here that computer game players can build high-quality crystal structures. Introduction of a new feature into the computer game Foldit allows players to build and real-space refine structures into electron density maps. To assess the usefulness of this feature, we held a crystallographic model-building competition between trained crystallographers, undergraduate students, Foldit players and automatic model-building algorithms. After removal of disordered residues, a team of Foldit players achieved the most accurate structure. Analysing the target protein of the competition, YPL067C, uncovered a new family of histidine triad proteins apparently involved in the prevention of amyloid toxicity. From this study, we conclude that crystallographers can utilize crowdsourcing to interpret electron density information and to produce structure solutions of the highest quality

Prevalence of major foliar and panicle diseases of Sorghum (Sorghum bicolor [L.] Moench) in the Deccan plateau of India

Extensive on-farm disease surveys were conducted from August 1999 until March 2001 in four sorghum-growing states of the Indian Deccan plateau. A total of 965 fields were surveyed covering 228 fields in Andhra Pradesh (AP), 406 in Karnataka (KAR), 290 in Maharashtra (MH) and 41 in Tamil Nadu (TN). Among 14 foliar diseases observed, maize stripe virus (MStV), a tenuivirus transmitted by the delphacid plant hopper (Peregrinus maidis), and among five panicle diseases, ergot or sugary disease (Claviceps sorghi and C. africana) were the most destructive diseases. MStV was prevalent in 28.4% and ergot in 13.4% of the fields surveyed in two years across four states. Yet, the mean incidence of MStV in AP was 6% with 85% mean severity. The values in KAR were 12% incidence and 83% severity, in MH 5% and 67%, and in TN 12% and 76%, respectively. The mean incidence of ergot in AP was 34% with 67% mean severity. The values in KAR were 41% and 79%, in MH, 30% and 67%, and in TN 100% and 100%, respectively. Variation in frequency of occurrence of MStV was observed between 1999 and 2001. Variations in frequency could be due to weather factors, vector survival, cropping pattern, and host specificity. The frequency of ergot also was varying among years, locations, seasons and cultivars. An ergot epidemic was observed during the 1999 rainy season in Maachinenipalli village (16°35′N; 78°3′E), Andhra Pradesh. In September 2000, the disease had spread to 13 neighboring administrative zones damaging about 130,000 ha. This paper elucidates the distribution of diseases observed between 1999 and 2001 but does not imply that the diseases are restricted necessarily to a particular zone or location.This article is published as Navi, S. S., Ranajit Bandyopadhyay, V. A. Tonapi, T. G. N. Rao, S. Indira, R. K. Reddy, P. W. Tooley, and D. Thomas. "Prevalence of major foliar and panicle diseases of Sorghum (Sorghum bicolor [L.] Moench) in the Deccan plateau of India." Archives of Phytopathology and Plant Protection 40, no. 1 (2007): 19-35. doi:10.1080/03235400500320232.</p