Determination of antioxidant, antimicrobial and antitumor activity of bryophytes from Mount Ida (Canakkale, Turkey)

395-401In this study, active components of bryophytes were extracted from the samples collected from Mount Ida (Canakkale- Turkey). Three different extraction methods (Soxhlet, supercritical fluid extraction-SFE, Soxhlet after SFE) were employed for 2016 samples while direct Soxhlet extraction was carried out for 2017 samples using methanol as extracting solvent. Initially, antioxidant activity tests of the crude extracts were performed for all the samples. Antioxidant properties were determined to be fairly weak or moderate in the majority of the species studied. Marchantia polymorpha (T1), a liverwort, showed high extraction yield and antioxidant activity. Similarly, high activity was detected in Hypnum cupressiforme (T9) and Neckera complanata (T10) species. The antioxidant capacity of the tested species often sheds light on other activities. Antimicrobial properties were also tested for all extracts obtained in 2017. Thuidium tamariscinum (T8) was positive against Acinetobacter haemolyticus ATCC 19002 bactericin; T1 and Isothceum myurum (T11) were positive against Bacillus subtilis TCC6633 bacteria and all other species were negative. Following these results, we have concentrated on the most active species and carried out antitumoral tests. Again, fractioned polar isolate of T1 was the only antiproliferative species against HeLa and A549 lung cancer cells

Türkiye' nin Ege ve Akdeniz Bölgesinden bazı karayosunu kayıtları

Bu çalışmada, Ege ve Akdeniz bölgelerinden 120 bitki örneği toplanmış olup 15 familya 36 cinse ait 56 takson saptanmıştır. Ditrichum pusillum (Hedw.) Hampe., Türkiye için ilk kez kaydedilmiştir.In this study, 120 specimens collected from the Aegean and Mediterranean region were identified with 56 taxa belonging to 15 families and 36 genera. Ditrichum pusillum (Hedw.) Hampe. has been recorded for the first time in Turkey

Muğla ve civarının karayosunu florası

Bu çalışmada bitki materyalleri 1993-1995 yılları arasında Muğla ve civarından toplanmıştır. Araştırma bölgesinde yapılan flora çalışmaları sonucunda 21 familyaya ait 53 cins 130 takson saptanmıştır. En çok takson içeren familyalar Pottiaceae 43 (%33.07), Bryaceae 17 (%13.07), Brachytheciaceae 16 (%12.30), Dicranaceae 9 (%6.92) ve Grimmiaceae 6 (%4.62) dir. En çok tür içeren cinsler ise Bryum 14 (%10.76), Barbula 11 (%8.46), Tortula 10 (%7.69), Grimmia 5 (%3.84) ve Orthotrichum 5 (%3.84)dur. Barbula enderesii Garov., Pohlia atropurpurea (Wahlenb.) Lindb., Bryum algovicum Sendt. ex C.Müll Türkiye ve Yakın ve Orta Doğu için yeni kayıttır.The plants presented in this study were collected in the vicinity of Muğla between 1993 -1995. As a result of botanic studies in the studied area 130 taxa and 53 genera belonging to 21 families were determined. The families Pottiaceae, Bryaceae, Brachytheciaceae, Dicranaceae and Grimmiaceae represent the highest number 43 (33.07 %), 17 (13.07 %), 16 (12.30 %), 9 (6.92 %) and 6 (4.62 %) respectively. The genera with the highest number of species are Bryum 14 (10.76 %), Barbula 11 (8.46 %), Tortula 10 (7.69 %), Grimmia 5 (3.84 %) and Orthotrichum 5 (3.84 %). Barbula enderesii Garov., Pohlia atropurpurea (Wahlenb.) Lindb., Bryum algovicum Sendt. ex C.Miill recorded for the first time in Turkey and the Near and Middle East

Protective effects of quercetin/moss extract (homalothecium sericeum hedw.) combination on STZ-induced diabetic rats

Diabetes Mellitus (DM) is the most common chronic endocrine disorder. Although new drugs and administration techniques for diabetes were developed, the nature of complications that occur chronically and reduce patients’ quality of life have not been entirely determined. In our study, 35 male rats were used. A single dose of 40 mg/kg streptozotocin was injected intraperitoneal into 28 rats, and a total of five groups were formed. The diabetes model was performed by applying 40mg/kg STZ and was not established in the subjects in the control and citrate buffer groups with STZ solvent. The quercetin and moss extract were given by gavage for three weeks. The liver and pancreas tissues were examined by histochemical and immunohistochemical methods. Ki-67 (proliferation marker), antinuclear factor kappa (NF-κB), and interleukin 6 (IL-6) immunohistochemically staining revealed significant differences in inflammation and regeneration in the liver and pancreas tissues. In addition, the TUNEL staining method was used to determine apoptosis. Positivity was significant between the treatment and diabetes groups. It was determined that the agents used as treatment changed serum superoxide dismutase (SOD), malondialdehyde (MDA), C-peptide, insulin, and glucose levels.BA

Investigation of Competitive and Noncompetitive Adsorption of Some Heavy Metals Ions on Leucodon sciuroides (Hedw.) Schwägr

Heavy metals are an important pollutant group. Adsorption is one of the methods used to remove heavy metals from the environment. Mosses were preferred as bio-indicators because they have the capacity to accumulate many elements by their high surface-to-volume ratio. Leucodon sciuroides (Hedw.) Schwägr. (LS) are mosses that play an important part of the ecosystem and are collected from the Ida Mountain (Kazdag) region of Çanakkale (Turkey). For the purpose of determining the adsorption capacity of heavy metal ion (Pb2+, Cd2+, Co2+, Ni2+, Zn2+, and Cu2+) analysis conditions, pH, contact time, and adsorbent amounts were determined and the maximum adsorption capacity was calculated with the help of the relevant isotherms. Heavy metal concentrations were determined by inductively coupled plasma-mass spectrometry. It was determined that the optimum adsorption for mosses was 30 min at pH = 6.0 (the pH at which maximum adsorption occurs). The adsorption event shows that some divalent cations fit the Freundlich isotherm and some fit the Langmuir isotherm model. A pseudo-second-order reaction best fits the kinetic data for metal ions. Among the six metal ions studied, the highest adsorption was observed in Pb2+ and Cu2+ cations. According to the competitive adsorption results, the moss has a great advantage in determining the Pb2+ and Cu2+ cations industrially as well as other metals and in removing other metal impurities from the environment. Also, LS is exploited as a biosorbent to remove metal ions from aqueous solutions and can be used as a biomarker