96 research outputs found

    The formation of homogentisate in the biosynthesis of tocopherol and plastoquinone in spinach chloroplasts

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    Homogentisate is the precursor in the biosynthesis of -tocopherol and plastoquinone-9 in chloroplasts. It is formed of 4-hydroxyphenylpyruvate of the shikimate pathway by the 4-hydroxyphenylpyruvate dioxygenase. In experiments with spinach the dioxygenase was shown to be localized predominatedly in the chloroplasts. Envelope membranes exhibit the highest specific activity, however, because of the high stromal portion of chloroplasts, 60–80% of the total activity is housed in the stroma. The incorporation of 4-hydroxyphenylpyruvate into 2-methyl-6-phytylquinol as the first intermediate in the tocopherol synthesis by the two-step-reaction: 4-Hydroxyphenylpyruvate Homogentisate 2-Methyl-6-phytylquinol was demonstrated by using envelope membranes. Homogentisate originates directly from 4-hydroxyphenylpyruvate of the shikimate pathway. Additionally, a bypass exists in chloroplasts which forms 4-hydroxyphenylpyruvate from tyrosine by an L-amino-acid oxidase of the thylakoids and in peroxisomes by a transaminase reaction. Former results about the dioxygenase in peroxisomes were verified

    Teores de aminoácidos e fenóis em plântulas de algodoeiro (Gossypium hirsutum L. cv. 'IAC-17') sob ação de reguladores vegetais

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    This research deals with the effects of exogenous growth regulators on dry weight, contents of aminoacids and phenolic compounds in leaves of cotton plants. To study the influence of the chemicals, cotton seeds were immersed during 22 hours in water solutions of (2-chloroethyl) trimethylammonium chloride (CCC) at concentration of 2000 ppm, succinic acid-2,2-dimethylhydrazide 4000 ppm,gibberellic acid 100 ppm, indolylacetic acid (IAA) 100 ppm, and water as check treatment. CCC 2000 ppm increased the dry weight in the leaves of cotton plants. This growth retardant increased the content of free aminoacids in cotton leaves. IAA 100 ppm reduced the concentration of aminoacids in the treated plants. The contents of phenolic compounds in leaves of cotton was increased by CCC, in relation to check treatment.Verificaram-se os efeitos da aplicação de reguladores de crescimento na porcentagem de materia seca, aminoácidos livres totais e compostos fenólicos nas folhas de algodoeiro 'lAC-l7'. Para se estudar a ação dos fitoreguladores, sementes de algodoeiro foram imersas durante 22 horas em soluções aquosas de cloreto (2-cloroetil) trimetilamônio (CCC) na concentração de 2000 ppm, ácido succínico-2,2-dimetilhidrazida 4000 ppm, ácido giberélico 100 ppm, ácido indolilacético (IAA) 100 ppm e água como controle. Plântulas com 33 dias de idade foram coletadas, sendo em seguida realizada a amostragem de folhas em laboratório, para efetuar-se a extração dos compostos. Observou-se que CCC 2000 ppm promoveu aumento no peso seco das folhas de algodoeiro. Esse retardador de crescimento aumentou o conteúdo de aminoácidos livres totais nas folhas estudadas. IAA 100 ppm reduziu a concentração de aminoácidos, nas plantas tratadas. O teor de fenóis totais nas folhas de Gossypium hirsutum foi aumentado por efeito do CCC com relação ao controle
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