Correlated Confocal and Intermediate Voltage Electron Microscopy Imaging of the Same Cells Using Sequential Fluorescence Labeling, Fixation, and Critical Point Dehydration

Confocal laser scanning microscopy (CLSM) and intermediate voltage transmission electron microscopy (IVEM) each has its own particular advantages. CLSM can examine living cells, but is particularly useful when applied to cells that have been lightly fixed, permeabilized, and stained with fluorescent-labeled antibodies for localization of specific molecular species at the resolution of the light microscope while still in the hydrated state. IVEM provides much higher resolution images, but requires more drastic preparation procedures, including dehydration. This paper presents methods for combining these complementary approaches to examine exactly the same cells sequentially by CLSM and IVEM. Cells are grown in culture on sterile formvar films spread over gold index grids on cover glasses, which are mounted on larger cover glasses or microscope slides with spacers to prevent compression of the cells. Light and epifluorescence microscopy, and CLSM are performed concentrating on cells in grid openings. Then the grids are fixed with aldehydes followed by OsO4, dehydrated and critical point dried (CPD) from liquid CO2. Immediately following CPD, the grids are ready for examination in the IVEM. Low magnification (300-600x) survey images allow correlation of the IVEM images with the light microscopic images. In higher power images, structures that are fluorescent labeled can be related to corresponding regions in the IVEM images

腎盂尿管移行部狭窄症に逆行性エンドピエロトミーを行った小児の1例

右腎盂尿管移行部狭窄症の6歳女児に対し, アキュサイス尿管切開バルーン装置(軟性尿管カテーテル, 7Fr)を用いた逆行性エンドピエトロトミーを行いその実用性を検討した.全身麻酔下でアキュサイスカテーテルを膀胱鏡操作下に腎盂内にまで挿入し, 狭窄部を電気的に切開し更に同部位をバルーンで24Frまで拡張した.切開終了後にエンドピエトロトミー用尿管カテーテル(6/10Fr)を留置し, これを8週間後に抜去した.手術時間は45分間で, 患者は術後3日目に退院した.特に手術に関する合併症はなく, 術後8ヵ月目の経静脈性腎盂撮影と利尿レノグラムによる評価で, 水腎症の程度は改善していたTo determine the feasibility of retrograde endopyelotomy in the management of pediatric ureteropelvic junction (UPJ) obstruction, we treated one girl aged 6 years with the Acucise cutting balloon devise for symptomatic UPJ obstruction. The Acucise catheter (7 Fr, flexible) was placed by a cystoscope over a guide wire with fluoroscopic guidance under general anesthesia. After cutting the stenotic area electronically and dilation until 24 Fr for 10 seconds, a 6/10 Fr endopyelotomy ureteral catheter was left in situ for 8 weeks after the operation. Total operating time was 45 minutes and the child was discharged 3 days after the operation. There were no acute complications and short-term, follow-up 8 months after the operative results were satisfactory as determined by intravenous pyelography and diuretic renogram. UPJ obstruction in children may be treated by retrograde endopyelotomy with the Acucise catheter as well as adults. The principal potential advantage of this procedure is reduced morbidity as compared with antegrade endopyelotomy

Topological Strings, Flat Coordinates and Gravitational Descendants

We discuss physical spectra and correlation functions of topological minimal models coupled to topological gravity. We first study the BRST formalism of these theories and show that their BRST operator $Q=Q_s+Q_v$ can be brought to $Q_s$ by a certain homotopy operator $U$, $UQU^{-1}=Q_s$ ($Q_s$ and $Q_v$ are the $N=2$ and diffeomorphism BRST operators, respectively). The reparametrization (anti)-ghost $b$ mixes with the supercharge operator $G$ under this transformation. Existence of this transformation enables us to use matter fields to represent cohomology classes of the operator $Q$. We explicitly construct gravitational descendants and show that they generate the higher-order KdV flows. We also evaluate genus-zero correlation functions and rederive basic recursion relations of two-dimensional topological gravity.Comment: 11 pages, phyzzx, UT-63

ICEF2011-60032 QUICK RESPONSE FUEL INJECTOR FOR DIRECT-INJECTION GASOLINE ENGINES

ABSTRACT We developed a new injector for direct injection gasoline engines that reduce the exhaust emissions and help to reduce fuel consumption. The newly developed actuator in this injector has two features. One is a bounce-less valve closing mechanism, and the second is quick-response moving parts. The first feature, the bounce-less valve closing mechanism, can prevent ejecting a coarse droplet, which causes unburned gas emission. The new actuation mechanism realizes the bounce-less valve closing. We analyzed the valve motion and injection behavior. The second feature, the quick response actuator, achieves a smaller minimum injection quantity. This feature assists in reducing the fuel consumption under low load engine conditions. The closing delay time of the needle valve is the dominant factor of the minimum injection quantity because the injection quantity is controlled by the duration time of the valve opening. The new actuator movements can be operated with a shorter closing delay time. The closing delay time is caused by a magnetic delay and kinematic delay. A compact magnetic circuit of the actuator reduces the closing delay time by 26%. In addition, the kinematic delay was improved when the hydraulic resistance was reduced by 9%. As a result, the new injector realizes reduction of the minimum injection quantity by 25% compared to a conventional injector

Factors Affecting the Gait Ability of the Home-bound Stroke Patients

Mediologische Lektüre von Friedrich Schillers Drama "Maria Stuart

Long-term observation of fibrillation cycle length in patients under angiotensin II receptor blocker therapy for chronic atrial fibrillation

AbstractIntroductionThe long-term effect of angiotensin II receptor blockers (ARBs) on atrial fibrillation (AF) is unclear. In this study, we evaluated the change in the fibrillation cycle length (FCL) in patients under long-term ARB therapy for chronic AF.Methods and resultsThe study population consisted of 25 chronic AF patients who were prescribed the same medication for more than 6 years and in whom specific ECG recording for FCL evaluation could be performed before and after the 6-year observation period. The patients were divided into 2 groups: those with and without ARB (ARB group and non-ARB group and n=15 and 10, respectively). FCL was calculated by the spectral analysis of the fibrillation waves in the surface ECG. There was no significant difference in the clinical characteristics between the 2 groups. In the ARB group, the mean FCL was prolonged from 154±20ms to 187±37ms (p=0.005), whereas it remained unchanged in the non-ARB group (150±12ms vs. 149±10ms). In the comparison between patients with and those without FCL prolongation (>30ms; n=6 and 19, respectively), a significant difference was observed only in those prescribed ARBs.ConclusionIn cases of chronic AF, FCL might be prolonged under long-term ARB treatment

Evaluation of the impact of atrial fibrillation on rehospitalization events in heart failure patients in recent years

SummaryBackgroundAlthough we have previously reported that the presence of paroxysmal atrial fibrillation (AF) is an independent risk factor for rehospitalization in patients with congestive heart failure (CHF) in a population from 1996 to 2002, the impact of AF configuration as a risk factor in a more recent population remains to be clarified.Methods and results319 patients with CHF admitted to our institute in 2006–2007 were retrospectively evaluated. The patients were divided into 3 groups in accordance with their basic cardiac rhythm, i.e. sinus rhythm (n=210), chronic AF (n=68), and paroxysmal AF (n=41). During the follow-up period of 19±17months, there was no significant difference in mortality or rehospitalization events among the 3 groups (p=0.542). In the multivariate analysis, no administration of β-blockers was the only independent risk factor for rehospitalization due to CHF exacerbation.ConclusionsThe clinical impact of AF configuration as a risk factor of rehospitalization due to CHF exacerbation was considered to be decreased in recent years

Minor Contribution of Quasars to Ionizing Photon Budget at z~6: Update on Quasar Luminosity Function at the Faint-end with Subaru/Suprime-Cam

We constrain the quasar contribution to cosmic reionization based on our deep optical survey of z~6 quasars down to z_R=24.15 using Subaru/Suprime-Cam in three UKIDSS-DXS fields covering 6.5 deg^2. In Kashikawa et al. (2015), we select 17 quasar candidates and report our initial discovery of two low-luminosity quasars (M_1450~ -23) from seven targets, one of which might be a Lyman alpha emitting galaxy. From an additional optical spectroscopy, none of the four candidates out of the remaining ten turn out to be genuine quasars. Moreover, the deeper optical photometry provided by the Hyper Suprime-Cam Subaru Strategic Program (HSC-SSP) shows that, unlike the two already-known quasars, the i-z and z-y colors of the last six candidates are consistent with M- or L-type brown dwarfs. Therefore, the quasar luminosity function (QLF) in the previous paper is confirmed. Compiling QLF measurements from the literature over a wide magnitude range, including an extremely faint AGN candidate from Parsa et al. (2017}, to fit them with a double power-law, we find that the best-fit faint-end slope is alpha=-2.04^+0.33_-0.18 (-1.98^+0.48_-0.21) and characteristic magnitude is M_1450^*=-25.8^+1.1_-1.9 (-25.7^+1.0_-1.8) in the case of two (one) quasar detection. Our result suggests that, if the QLF is integrated down to M_1450=-18, quasars produce ~1-12% of the ionizing photons required to ionize the whole universe at z~6 with 2sigma confidence level, assuming that the escape fraction is f_esc=1 and the IGM clumpy factor is C=3. Even when the systematic uncertainties are taken into account, our result supports the scenario that quasars are the minor contributors of reionization.Comment: 8 pages, 3 figures, ApJL accepte