Towards a healthier cannabis? Examining neurobehavioural interactions between THC and CBD in mice

Abstract Cannabis is the most widely used illicit drug in the world, however over the past decade there has been increasing interest in its utility as a potential therapeutic. Despite the reported beneficial effects of cannabis constituent Δ9-tetrahydrocannabinol (THC), its psychoactivity has curtailed its therapeutic use. There has been increasing interest in combining non-psychoactive cannabis constituent cannabidiol (CBD with THC to inhibit THC’s adverse effects, leading to generation of medications which contain ~1:1 CBD to THC dose ratios which are currently used for the treatment of spasticity in multiple sclerosis and pain relief. However there is a limited evidence base regarding to what extent CBD might modulate the pharmacological effects of THC at equal doses. The scientific examination of pharmacological interactions between these chemicals is therefore of major medical and public health significance. This thesis examines whether an equivalent dose of CBD is able to ameliorate the neuropharmacological effects of THC in mice following acute and repeated dosing in adulthood and adolescence, using doses relevant to human consumption. We report that CBD acutely inhibited some (but not all) of the neurobehavioural measures taken, suggesting potential benefits of CBD in reducing the unwanted effects of THC. However, the unexpected activation of mesolimbic circuitry when THC and CBD were combined suggests enthusiasm should be tempered until these effects are better understood. Adolescent mice exposed to a modest THC dose equivalent to most recreational and medicinal users did not display long-term behavioural deficits, and hence no reversal of negative outcomes by CBD could be measured. CBD alone produced no behavioural changes following acute or repeated exposure in adult mice, although adolescent CBD exposure reduced depression-like behaviour in adult mice, an intriguing effect warranting further study

Cookbook Recipes for Participants in Grady\u27s Fruit and Vegetable Prescription Program

Wholesome Wave, Open Hand Atlanta, and Grady Health Systems are working together to run a Fruit and Vegetable Prescription Program for patients in the Atlanta area. The program provides nutrition education, cooking classes, and money for patients to spend at the MARTA Farmer’s Market based on the number of people in their household. The purpose of this project is to create a cookbook of healthy vegetable side dishes using ingredients that are in season and could potentially be found at the markets to be utilized by patients participating in the program

Maiden Voyage: A Library and Faculty Development Center Partnership to Promote Information Literacy

Incorporating information literacy skills and competencies into the curriculum requires collaborative partnerships with the library faculty and the academic faculty with whom they work. At Northern Kentucky University’s Steely Library, a non-tenure track faculty position was created to serve in a dual role as a member of the Information Literacy faculty in the library and a member of the faculty in the University’s Professional and Organizational Development Center (POD). This dual role allows for the librarian to gain valuable insight to faculty needs while serving as a faculty member of the POD. Giving teaching faculty the opportunity to encounter the concept of information literacy in a variety of contexts, allows them to become more interested and open to exploring how it can enhance their teaching and student learning. Areas in which the library can enhance faculty understanding and incorporation of the library’s information literacy curriculum are more easily recognized with the librarian embedded into the POD. Targeted and specific information literacy tools such as tutorials, web pages, and blackboard courses are developed and promoted via the POD. The library\u27s information literacy initiatives are marketed via the POD, therefore increasing the library presence among academic faculty. As a member of the library’s Information Literacy and Instruction Team, the librarian is able to report findings from POD activities and use this information to increase the success of the library’s information literacy and instruction programs. This presentation will outline the strategies of this collaborative partnership and describe how it has impacted the integration of information literacy skills into the curriculum

Shock volume: Patient-specific cumulative hypoperfusion predicts organ dysfunction in a prospective cohort of multiply injured patients

BACKGROUND: Multiply injured patients are at risk of developing hemorrhagic shock and organ dysfunction. We determined how cumulative hypoperfusion predicted organ dysfunction by integrating serial Shock Index measurements. METHODS: In this study, we calculated shock volume (SHVL) which is a patient-specific index that quantifies cumulative hypoperfusion by integrating abnormally elevated Shock Index (heart rate/systolic blood pressure ≥ 0.9) values acutely after injury. Shock volume was calculated at three hours (3 hr), six hours (6 hr), and twenty-four hours (24 hr) after injury. Organ dysfunction was quantified using Marshall Organ Dysfunction Scores averaged from days 2 through 5 after injury (aMODSD2–D5). Logistic regression was used to determine correspondence of 3hrSHVL, 6hrSHVL, and 24hrSHVL to organ dysfunction. We compared correspondence of SHVL to organ dysfunction with traditional indices of shock including the initial base deficit (BD) and the lowest pH measurement made in the first 24 hr after injury (minimum pH). RESULTS: SHVL at all three time intervals demonstrated higher correspondence to organ dysfunction (R2 = 0.48 to 0.52) compared to initial BD (R2 = 0.32) and minimum pH (R2 = 0.32). Additionally, we compared predictive capabilities of SHVL, initial BD and minimum pH to identify patients at risk of developing high-magnitude organ dysfunction by constructing receiver operator characteristic curves. SHVL at six hours and 24 hours had higher area under the curve compared to initial BD and minimum pH. CONCLUSION: SHVL is a non-invasive metric that can predict anticipated organ dysfunction and identify patients at risk for high-magnitude organ dysfunction after injury. LEVEL OF EVIDENCE: Prognostic study, level III

Efficacy of the Unified Protocol for transdiagnostic treatment of comorbid psychopathology accompanying emotional disorders compared to treatments targeting single disorders

OBJECTIVE: This study aimed to examine whether the Unified Protocol (UP), a transdiagnostic cognitive-behavioral therapy for emotional disorders (i.e., anxiety, mood, and related disorders), is efficacious in the treatment of co-occurring emotional disorders compared to established single disorder protocols (SDPs) that target specific disorders (e.g., panic disorder). METHOD: Participants included 179 adults seeking outpatient psychotherapy. Participant age ranged from 18 to 66 years, with an average of 30.66 years (SD = 10.77). The sample was 55% female and mostly Caucasian (83%). Diagnostic assessments were completed with the Anxiety Disorder Interview Schedule (ADIS), and disorder-specific, clinician-rated measures for the comorbid diagnoses of interest. RESULTS: In both treatment conditions, participants' mean number of diagnoses dropped significantly from baseline to posttreatment, and baseline to 12-month follow-up. Additionally, large effects were observed for changes in comorbid generalized anxiety (ESɢ: UP = −1.72; SDP = −1.98), social anxiety (ESɢ: UP = −1.33, −0.86; SDP = −1.60, −1.54), and depression (ESɢ: UP = −0.83; SDP = −0.84). Significant differences were not observed in between-group comparisons. CONCLUSIONS: Results suggest that both the UP and SDPs are efficacious in reducing symptoms of comorbid emotional disorders. The clinical, practical, and cost-effective advantages of transdiagnostic CBT are discussed.Please note the following financial disclosures/conflicts of interest: Dr. Barlow reported receiving royalties from Oxford University Press, United Kingdom (which includes royalties for the treatment manuals included in this study); Guilford Publications Inc., United States; Cengage Learning, United States; Pearson Publishing, United Kingdom. He reported receiving grants from the National Institute of Mental Health, United States (R01 MH090053) and the National Institute of Alcohol and Alcohol Abuse, United States (R01 AA023676). He reported serving as a consultant for and receiving honoraria from the Agency for Healthcare Research and Quality, United States; the Foundation for Informed Medical Decision Making, United States; the Department of Defense, United States; the Renfrew Center, United States; the Chinese University of Hong Kong, Hong Kong;Universidad Catolica de Santa Maria, Peru); New Zealand Psychological Association, New Zealand; Hebrew University of Jerusalem, Israel; Mayo Clinic, United States; and various American universities. (R01 MH090053 - National Institute of Mental Health, United States; R01 AA023676 - National Institute of Alcohol and Alcohol Abuse, United States)Accepted manuscrip

Kinetochore Attachments Require an Interaction between Unstructured Tails on Microtubules and Ndc80Hec1

SummaryKinetochore attachments to microtubules are tight enough to move chromosomes, yet the microtubules' plus ends must remain dynamic and reposition within the attachment pocket during depolymerization-coupled movement. Kinetochores are unable to bind microtubules after any of the four subunits of the Ndc80 complex are knocked down [2, 4]; however, because the Ndc80 complex has important structural roles [1–3], it is unclear whether it directly mediates kinetochore-microtubule attachments. The Ndc80Hec1 subunit (Hec1) has a microtubule-binding site composed of both an unstructured N-terminal tail and a calponin homology domain [5–7]. Here, we show that, surprisingly, the N-terminal tail is sufficient for microtubule-binding affinity in vitro. The interaction is salt sensitive, and the positively charged Hec1 tail cannot bind microtubules lacking negatively charged tails. We have replaced the endogenous Hec1 subunit with a mutant lacking the N-terminal tail. These cells assemble kinetochores properly but are unable to congress chromosomes, generate tension across sister kinetochores, or establish cold-stable kinetochore-microtubule attachments. Our data argue that the highest affinity interactions between kinetochores and microtubules are ionic attractions between two unstructured domains. We discuss the importance of this finding for models of repositioning of microtubules in the kinetochore during depolymerization

Promoting Sustainable Transportation with Campus Car Policies and Public Outreach

In fall of 2016 at California State University Monterey Bay (CSUMB), the Environmental Studies undergraduate program began offering its first group-based capstone course that was based on its first Projects for Sustainable City Year course (ENSTU 471). Capstone projects are a senior-level, project based graduation requirement for an undergraduate degree. The Sustainable City Year projects focused on increasing sustainable transportation at two locations; one in the nearby city of Salinas, California, and one on the CSUMB campus. A majority of the community in Salinas drive which has limited the quality and effectiveness of the shuttle system provided by Monterey-Salinas Transit (MST). Our group seeks to help increase ridership and promote sustainable transportation in this region. At CSUMB, traffic congestion on campus is increasingly problematic as the student body continues to row. The majority of students drive to campus in single passenger vehicles which increases the traffic on campus. Sustainable transportation via buses, bicycling, and carpooling helps decrease traffic congestion. In the following paper, the CSUMB project covers a proposed freshman vehicle restriction policy to encourage sustainable modes of transportation and a bus-bicycle culture. For the Salinas project, we worked directly with MST to encourage common knowledge of bus usage by creating an informational and interactive booklet