Hair Pigmentation: A Research Update

Hair is a uniquely mammalian trait with important functions, most easily appreciated in furred mammals. Our skin and hair color contribute very significantly to our overall visual appearance by highlighting striking variations between human sub-groups. Although melanins, hemoglobins, and carotenoids define the color perceived at the skin surface, our hair color relies only on the presence or absence of different melanins. The hair shaft's physical aspects provide only minor color modification. Various selective evolutionary pressures have determined that within the context of our specific ethnic backgrounds a bewildering array of natural shades are seen; ranging from yellows, reds, and browns to black and that harbinger of lost youth, gray/white hair. Skin/hair follicle melanins are formed in cytoplasmic organelles called melano-somes produced by neural crest-derived pigment cells called melano-cytes and are the product of a complex, phylogenetically ancient, biochemical pathway called melano-genesis. The following provides a review of research presented at the 4th Intercontinental Meeting of Hair Research Societies 2004 and so is not intended to represent a fully comprehensive overview of the subject—for that readers are directed to key references

Hitting the Threshold of Common Sense: The Time for Screening Limits to Guard Against Environmental Transfer Is Now

Recreational drug use and the opiate epidemic have taken a great toll on the human population of the United States and beyond. Year over year, deaths from synthetic opioids—of which fentanyl ranks as the most prevalent—have skyrocketed, reaching 29,406 in 2017. Environmental transfer of drugs of human addiction to horses is nothing new. Cocaine and its primary metabolite, benzoylecgonine, have long been identified as environmental substances in post-race samples, and many jurisdictions have screening limits in place as a result. More recently, methamphetamine has begun to show up in post-race samples, reflecting the increasing addiction problem associated with this drug. In recognition of this growing problem, the National HBPA and North American Association of Racetrack Veterinarians made a Model Rule recommendation to the Association of Racing Commissioners International for screening limits for substances of human addiction in racing horses

Self-lubricating polymer composites : using numerical trbology to hightlight their design criterion

International audienceAfter the cessation of RT/Duroid 5813, manufacturing tests were performed by CNES and ESA/ESTL in order to find an alternative material. Although PGM-HT was selected as the best candidate, limitations about its tribological capabilities to replace RT/Duroid 5813 were later pointed out. Today, the predictability of the tribological behaviour of those materials is not fully overcome. The motivation to this work is to complement studies of self-lubricating materials by coupling experimental analyses with numerical modelling, in order to predict their tribological behaviour. A Discrete Element Method is chosen to construct the numerical material, because it allows to represent wear and the third body generation at the scale of the ball/retainer contact. An underlying role of the adhesion between components in controlling the tribological properties of the transfer film has been observed

Self-lubricating composite bearings: Effect of fibre length on its tribological properties by DEM modelling

International audienceSelf-lubricating polymer-based composites are used in space and in aircraft mechanisms as materials for solid lubricated systems. Such composites mostly consist of a polymeric matrix and fillers of two kinds: hard fillers (fibres made of glass, or of minerals) and solid lubricating particles (made of MoS 2). Their advantages are that they provide their own lubrication, and they can be used in both very high and very low temperatures (from −40 up to ~200 F). Precision ball bearings with these composites are manufactured since the 60's in these bearings the retainer material itself provides the lubrication. From the experimental analyses implemented (X-ray tomography, SEM observations, and experiences in a tribometer); it is possible to observe that the geometry of the fillers has a strong influence on the third body rheology. Nevertheless, the confined nature of the contact does not allow in-situ observation. To overcome this difficulty a combined numerical/experimental approach is carried out. To be able to reproduce the evolution of third-body particles within the contact, Discrete Element Methods (DEM) is used. Such an approach allows to represent wear: by the construction of an equivalent continuous medium resulting from the incorporation of interaction laws between the discrete particles. The motivation to this work is the understanding of the impact of filler geometry o tribological behaviour of these materials. More specifically, the goal is to study the influence of the fibre length in the tribological behaviour of self-lubricating composites by Discrete Element Methods (DEM)

SNARE-Dependent Membrane Fusion Initiates α-Granule Matrix Decondensation in Mouse Platelets

Platelet α-granule cargo release is fundamental to both hemostasis and thrombosis. Granule matrix hydration is a key regulated step in this process, yet its mechanism is poorly understood. In endothelial cells, there is evidence for 2 modes of cargo release: a jack-in-the-box mechanism of hydration-dependent protein phase transitions and an actin-driven granule constriction/extrusion mechanism. The third alternative considered is a prefusion, channel-mediated granule swelling, analogous to the membrane “ballooning” seen in procoagulant platelets. Using thrombin-stimulated platelets from a set of secretion-deficient, soluble N-ethylmaleimide factor attachment protein receptor (SNARE) mutant mice and various ultrastructural approaches, we tested predictions of these mechanisms to distinguish which best explains the α-granule release process. We found that the granule decondensation/hydration required for cargo expulsion was (1) blocked in fusion-protein-deficient platelets; (2) characterized by a fusion-dependent transition in granule size in contrast to a preswollen intermediate; (3) determined spatially with α-granules located close to the plasma membrane (PM) decondensing more readily; (4) propagated from the site of granule fusion; and (5) traced, in 3-dimensional space, to individual granule fusion events at the PM or less commonly at the canalicular system. In sum, the properties of α-granule decondensation/matrix hydration strongly indicate that α-granule cargo expulsion is likely by a jack-in-the-box mechanism rather than by gradual channel-regulated water influx or by a granule-constriction mechanism. These experiments, in providing a structural and mechanistic basis for cargo expulsion, should be informative in understanding the α-granule release reaction in the context of hemostasis and thrombosis

Distinct phosphorylation sites on the ghrelin receptor, GHSR1a, establish a code that determines the functions of ß-arrestins.

The growth hormone secretagogue receptor, GHSR1a, mediates the biological activities of ghrelin, which includes the secretion of growth hormone, as well as the stimulation of appetite, food intake and maintenance of energy homeostasis. Mapping phosphorylation sites on GHSR1a and knowledge of how these sites control specific functional consequences unlocks new strategies for the development of therapeutic agents targeting individual functions. Herein, we have identified the phosphorylation of different sets of sites within GHSR1a which engender distinct functionality of ß-arrestins. More specifically, the Ser(362), Ser(363) and Thr(366) residues at the carboxyl-terminal tail were primarily responsible for ß-arrestin 1 and 2 binding, internalization and ß-arrestin-mediated proliferation and adipogenesis. The Thr(350) and Ser(349) are not necessary for ß-arrestin recruitment, but are involved in the stabilization of the GHSR1a-ß-arrestin complex in a manner that determines the ultimate cellular consequences of ß-arrestin signaling. We further demonstrated that the mitogenic and adipogenic effect of ghrelin were mainly dependent on the ß-arrestin bound to the phosphorylated GHSR1a. In contrast, the ghrelin function on GH secretion was entirely mediated by G protein signaling. Our data is consistent with the hypothesis that the phosphorylation pattern on the C terminus of GHSR1a determines the signaling and physiological output

Evaluating Greek equity funds using data envelopment analysis

This study assesses the relative performance of Greek equity funds employing a non-parametric method, specifically Data Envelopment Analysis (DEA). Using an original sample of cost and operational attributes we explore the e¤ect of each variable on funds' operational efficiency for an oligopolistic and bank-dominated fund industry. Our results have significant implications for the investors' fund selection process since we are able to identify potential sources of inefficiencies for the funds. The most striking result is that the percentage of assets under management affects performance negatively, a conclusion which may be related to the structure of the domestic stock market. Furthermore, we provide evidence against the notion of funds' mean-variance efficiency