Panel tribina održana je 17.05.2011. sa temom “Stop yo–yo – Kako zdravo mršavjeti?” (Public discussion was held on 17 May 2011 with the theme „Stop yo-yo – How to loose weight healthy?“)

Pregled tribine: Tea Tošić i Sonja NjunjićVerificirao: prof.dr.sc. Mirko KoršićTribina je organizirana sa željom da se potakne javnost i politička struktura države da prepoznaju debljinu kao bolest. Dr. Koršić, kao jedan od utjecajnijih liječnika što se problema povezanih s debljinom tiče, pristao je preuzeti ulogu moderatora.Panelisti:-prof.dr.sc. Mirko Koršić, FRCP, endokrinolog, KBCZ, predsjednik Hrvatskog društva za debljinu-dr.sc. Jozo Jelčić, endokrinolog, voditelj ambulante za debljinu, Hr. društvo za debljinu-prof dr. sc. Željko   Metelko, dijabetolog, Referentni centar za dijabetes Vuk Vrhovac-dr. Lidija Hrastić, dopročelnica Gradskog ureda za zdravstvo-dr. Sanja Predavec, načelnica Odjela za preventivnu medicinu Ministarstva zdravstva-dr. Mimi Vurdelja, nutriciologinja, Hrvatski olimpijski odbor-Medikor, firma za plasman proizvoda   za zdraviji život, Branimir Dolibašić, nutricionist-Milsing, firma za plasman nadomjestaka hrani i vitamina, dr. Lana Rnjak-dr. Darko Vrčić, epidemiolog, čakovec-mag.pharm, M. Pišćetak i J. Vugec-Mihok, predstavnice Pharmacij

Panel tribina održana je 17.05.2011. sa temom “Stop yo–yo – Kako zdravo mršavjeti?” (Public discussion was held on 17 May 2011 with the theme „Stop yo-yo – How to loose weight healthy?“)

Pregled tribine: Tea Tošić i Sonja NjunjićVerificirao: prof.dr.sc. Mirko KoršićTribina je organizirana sa željom da se potakne javnost i politička struktura države da prepoznaju debljinu kao bolest. Dr. Koršić, kao jedan od utjecajnijih liječnika što se problema povezanih s debljinom tiče, pristao je preuzeti ulogu moderatora.Panelisti:-prof.dr.sc. Mirko Koršić, FRCP, endokrinolog, KBCZ, predsjednik Hrvatskog društva za debljinu-dr.sc. Jozo Jelčić, endokrinolog, voditelj ambulante za debljinu, Hr. društvo za debljinu-prof dr. sc. Željko   Metelko, dijabetolog, Referentni centar za dijabetes Vuk Vrhovac-dr. Lidija Hrastić, dopročelnica Gradskog ureda za zdravstvo-dr. Sanja Predavec, načelnica Odjela za preventivnu medicinu Ministarstva zdravstva-dr. Mimi Vurdelja, nutriciologinja, Hrvatski olimpijski odbor-Medikor, firma za plasman proizvoda   za zdraviji život, Branimir Dolibašić, nutricionist-Milsing, firma za plasman nadomjestaka hrani i vitamina, dr. Lana Rnjak-dr. Darko Vrčić, epidemiolog, čakovec-mag.pharm, M. Pišćetak i J. Vugec-Mihok, predstavnice Pharmacij

Advanced Teaching Methods Application and its Benefits in Descriptive Geometry at the Faculty of Civil Engineering and Architecture in Niš

Teaching tools, such as computer drawings in lecture templates, "step by step" presentations, Android application, got introduced so as to improve teaching process quality in Descriptive geometry subject, at the Faculty of Civil Engineering and Architecture in Niš. Advanced teaching methods in practice classes, and on-the-screen video beam projected "step by step" presentations were available only to half of the enrolled students (experimental group –the EG), while the rest attended the practice classes deploying classical teaching methods, and manual drawings on the blackboard (control group –the CG). Deliberation of data on previous education and intellectual capabilities of students involved proved that the groups do not differ. Comparative analysis of the final results in academic year of 2016/17 indicated that in the EG students there was a higher knowledge of the subject matter, and higher exam pass rate. The conclusion is that the application of the advanced teaching methods makes learning and teaching process more successful, thus their usage in the future is recommended

Effect of steroids on transcription and secretion of Gal-1 by the human trophoblast cell line in vitro

Galectin-1 (Gal-1) is a lectin with recently documented pro-invasive function in trophoblasts in vitro, whose regulation is currently insufficiently known. The potential involvement of steroid hormones, synthetic glucocorticoid dexamethasone (DEX), the sex steroid progesterone (PRG) and mifepristone (RU486) in the regulation of Gal-1 in the trophoblast-derived cell line HTR-8/SVneo was investigated. Gal-1 mRNA levels were assessed by real-time PCR. The effect on secretion of Gal-1 into the culture media was followed using the SELDI-TOF protein chip array. We present evidence that DEX and RU486 significantly reduced Gal-1 in the HTR-8/SVneo cell line at the mRNA level. In addition, trophoblast-derived HTR-8/SVneo cells were shown to secrete detectable Gal-1 protein, which was only slightly increased by PRG. The potential clinical relevance of these findings remains to be determined. [Projekat Ministarstva nauke Republike Srbije, br. 173004

Local collections in Croatian public libraries

U radu se razmatra problematika zavičajnih zbirki u hrvatskim narodnim knjižnicama i to na sljedeći način: u prvome jedijelu rada izložen pregled dosad objavljenih teorijskih radova hrvatskih autora o zavičajnim zbirkama u narodnim knjižnicama, prikazani su hrvatski zakonski i podzakonski akti koji donose propise vezane uz zavičajne zbirke u narodnim knjižnicama ; u drugome dijelu rada predstavljeni su statistički podatci o zavičajnim zbirkama u narodnim knjižnicama - prikazan je broj narodnih knjižnica koje imaju zavičajne zbirke, broj knjiga u svescima, zatim podatci o nabavi i korištenju građe, za što su korišteni statistički podatci prikupljeni u Hrvatskome zavodu za knjižničarstvo u Nacionalnoj i sveučilišnoj knjižnici u Zagrebu

CONSTRUCTIVE SYSTEM OF RECIPROCAL FRAMES IN TERMS OF CONTEMPORARY ARCHITECTURE

In this paper, the reciprocal frame constructions are analyzed, starting from their definition, historical survey, to static and geometric characteristics, types and shapes. Although very familiar in their shape, their usage earlier was not the same as now in contemporary architecture. An overview of some of the more recent examples of conceptual solutions and derived pavilions has been established. These structures represent good example for temporary objects in a form of pavilions. Goal in this paper is to design a sample of a closed permanent object of these structures with combining classical structural systems and RFs. Chosen geometry was predefined as regular in order to use its properties to determine if there is a possibility to form equal units. Finally, based on defined entities, the pavilion’s conceptual design was made using parametric design in plug-in Grasshopper and Rhinoceros software

Application of Next-Generation Sequencing Technology and Establishment of Biobanks

Institute of Molecular Genetics and Genetic Engineering has become widely recognized as an expert centre for rare diseases (RD). It is the first institution is Serbia that applied NGS methodology in research and diagnostics of RD. We are also the institution with RD biobank collections containing DNA, RNA, mononuclear cells and tissue samples from over 2000 of patients affected with 50 different diseases. An accurate diagnosis was provided to over than 100 RD patients who were undiagnosed for years. We used Clinical-Exome Sequencing TruSightOne Gene Panel (4813 clinicaly-relevant genes), Illumina MiSeq instrument and Illumina VariantStudio. For monogenic diseases, filtration and prioritization of variants were performed according to “in-house” pipeline, using virtual gene panels. Variants were analyzed by various in silico softwares and classified according to ACMG guidelines. Variants selected by these criteria were confirmed by conventional Sanger sequencing and parents’ samples were analyzed whenever available. Furthermore, novel variants in DNAI1, MUT, PAH, PCCB, SLC37A4, SPAG16 and SPAG17 genes were functionally characterized in adequate in vitro systems such as immortalized patients’ fibroblasts or CRISPR /Cas9 edited commercial cell lines. Clinical-exome sequencing enabled diagnosis of more than 50 different diagnosis (hematological, metabolic, endocrinological, pulmonary, immunological, orthopedic, dermatological, ophthalmological, cardiological, epileptic encephalopathies etc.). It was particularly important for genetically heterogeneous diseases, such as glycogen storage diseases, branched-chain organic acidurias, primary ciliary dyskinesia, MODY or mitochondriopathies. Moreover, different diseases with overlapping clinical manifestations were accurately diagnosed. Also, we used TruSeq-Amplicon Cancer Panel to analyse different childhood and adult rare hematological malignancies. Besides studying diagnostic and prognostic malignancy markers, we designed “in-house” virtual pharamocogenomic panel, and performed association studies of pharmacogenomic markers and the course and outcome of rare hematological malignancies, resulting in recommendations for therapeutic modalities in accordance with genomic profile of the patien

Effect of steroids on transcription and secretion of Gal-1 by the human trophoblast cell line in vitro

Galectin-1 (Gal-1) is a lectin with recently documented pro-invasive function in trophoblasts in vitro, whose regulation is currently insufficiently known. The potential involvement of steroid hormones, synthetic glucocorticoid dexamethasone (DEX), the sex steroid progesterone (PRG) and mifepristone (RU486) in the regulation of Gal-1 in the trophoblast-derived cell line HTR-8/SVneo was investigated. Gal-1 mRNA levels were assessed by real-time PCR. The effect on secretion of Gal-1 into the culture media was followed using the SELDI-TOF protein chip array. We present evidence that DEX and RU486 significantly reduced Gal-1 in the HTR-8/SVneo cell line at the mRNA level. In addition, trophoblast-derived HTR-8/SVneo cells were shown to secrete detectable Gal-1 protein, which was only slightly increased by PRG. The potential clinical relevance of these findings remains to be determined

Effects of DMSO, glycerol, betaine and their combinations in detecting single nucleotide polymorphisms of epidermal growth factor receptor (EGFR) gene promoter sequence in non-small-cell lung cancer (NSCLC) patients

The aim of the study was to examine the effects of frequently used polymerase chain reaction (PCR) additives DMSO, glycerol and betaine on amplification of GC-rich epidermal growth factor receptor (EGFR) gene promoter region, in order to detect the presence of -216G gt T and -191C gt A gene variations in non-small-cell lung cancer (NSCLC) patients. PCR products and restriction fragments were detected by electrophoresis on 8% polyacrylamide gel and 3% agarose gel. Our analysis shows that single used additives including DMSO in concentration of 7% and 10%, glycerol in concentration of 10%, 15% and 20%, as well as betaine in concentration of 1 M, 1.5 M and 2 M significantly enhanced the yield and specificity of PCR reaction. In addition, the combination of 10% DMSO with 15% glycerol has shown positive effects, whereas other analyzed combinations of additives failed to amplify the EGFR promoter region.This is the peer reviewed version of the paper: Jurišić, V., Obradović, J., Tošić, N., Pavlović, S., Kulić, M., & Djordjević, N. (2016). Effects of DMSO, glycerol, betaine and their combinations in detecting single nucleotide polymorphisms of epidermal growth factor receptor (EGFR) gene promoter sequence in non-small-cell lung cancer (NSCLC) patients. Journal of Pharmaceutical and Biomedical Analysis, 128, 275–279. [https://doi.org/10.1016/j.jpba.2016.05.010][https://www.sciencedirect.com/science/article/abs/pii/S0731708516302473?via%3Dihub

Reversal of FLT3 Mutational Status and Sustained Expression of NPM1 Mutation in Paired Presentation, and Relapse Samples in a Patient with Acute Myeloid Leukemia

We report a case of de novo acute myeloid leukemia (AML) with unstable FLT3 gene mutations and stable NPM1 mutation. FLT3/D835 and NPM1 (Type A) mutations were detected upon diagnosis. During the relapse, the FLT3/D835 mutation changed to an FLT3/ITD mutation while the NPM1 (Type A) mutation was retained. Cytogenetic analyses showed the normal karyotype at diagnosis and relapse. Our findings raise interesting questions about the significance of these mutations in the leukemogenic process, about their stability during the evolution of the disease, and regarding the selection of appropriate molecular markers for the monitoring of minimal residual disease