Ekstrak Keladi Tikus (Typhonium Flagelliforme) Fraksi Diklorometanolik dan Ekspresi Caspase-3 dan P21 Cell-Line Kanker Payudara MCF-7

The dichloromethanolic fraction of keladi tikus (Typhonium flagelliforme) extract and the expression of p21 and caspase-3 of MCF-7 breast cancer cell-lineBackground: Breast cancer has a high degree of heterogeneity and problems in therapy, such as insensitive to antiproliferation signal and able to escape from apoptosis program. Typhonium flagelliforme (Lodd), Blume (Araceae) more commonly known as keladi tikus in Indonesia, often used as alternative in treating cancer, including breast cancer. The objective of study is proving the dichloromethanolic fraction of Typhonium flagelliforme tuber extracts influence on the expression of caspase-3 and p21 of MCF-7 cell-line that have deletion in CASP-3 gene.Methods: This study adapts laboratory experimental in-vitro in cells-line MCF-7, with “post test control group only design” and divided into two groups, were incubated in 5h, 10h and 20h. The control group received no other treatment. The treatment group received the dichloromethanolic fraction of Typhonium flagelliforme tuber extracts in IC50. Starting with cytotoxicity assay using MTT to determine the IC50, followed by Immunocytochemical analysis to observe the expression of p21 and caspase-3.Results: Paired sample T test shows a significant differences in treatment groups compared with the controls (p<0.05), namely an increase in the expression of nuclear p21 and caspase-3 on 20h incubation.Conclusion: There were increased in the expression of caspase-3 and nuclear p21, as well as depleted the cytoplasmic of p21 on MCF-7 cell-line that have deletion in CASP-3 in treatment by tuber extract of Typhonium flagelliforme dichloromethanolic fraction

Upaya Peningkatan Keselamatan Pada Jalan Nasional Indonesia

Indonesia faced serious challenges in improving traffic safety on the road. Government policy breakthrough has been made by issuing Presidential Decree No. 4 of 2013 of the Decade of Action for Road Safety 2011-2020 which is also in line with the National Road Safety Plan which was declared in 2011. Efforts to improve the safety of roads have been initiated through the road worthiness test. However, the results are generally conditionally accepted and facts on the field indicate that the number of accidents is still high. This paper discusses efforts to improve road safety and strategies that should be done on a national road, by conducting a road safety audit based on traffic accident data obtained from the Integrated Road Safety Management System which is owned Korlantas and understand the anatomy of the existing accident and behavioral factors of the society along the road through an audit. A case study was conducted on the Java North Coast Corridor, between Subang and Indramayu of West Java

Pengaruh Pemberian Ekstrak Phyllanthus Niruri Linn terhadap Infiltrasi Limfosit dan Ekspresi Perforin pada Kanker Kolon Tikus Sprague-Dawley

The effect of Phyllanthus niruri Linn extract on lymphocytes infiltration and perforin expression in colon cancer of Sprague-Dawley RatBackground: Colon cancer treatment currently involves immunotherapy that aims to improve the quality of life and survival of patients. Phyllanthus niruri Linn (P. niruri L) may act as an immunomodulator, but its potency in antitumor immune responses has not been revealed. The study was conducted to evaluate the differences between the immunological status of rats suffering colon cancer which were not given to those given the extract of P. niruri L.Methods: The study was randomized posttest-only control group design. Samples were 30 Sprague-Dawley male rats, bodyweight 170-220 gr which induced by 1.2 DMH 30 mg/kgBW subcutaneously. On the weeks 9, 11 and 13, four induced rats each week were sacrified to detect the development of colon cancer. On the weeks of 13th all of 4 rats were developed colon cancer, so the induction were stopped. The rest of 18 induced rats were randomly into two groups: without P. niruri L or positive control (K+)=9 rats and given P. niruri L extract 13.5 mg/kg orally or X group=9 rats. After 19th week all of rats were then terminated and tumor lesion of colon were processed hystophatologically. The tissues of colon cancer were stained by H&E for evaluate the lymphocytes infiltration and immunohistochemistry monoclonal antibody anti-perforin for perforin expression. Non pairs t-test was used with considered significant if p<0.05.Results: The mean of lymphocytes infiltration of the group X was 401.89±70.19, it was higher compared to K+ 191.89±50.68 (p=0.000). The mean percentage of perforin expression of group X was 39.00±1.80%, it was higher compared to K+ 23.00± 3.00% (p=0.000). Conclusion: The extract of P. niruri L increases immunologic status through mechanism of lymphocytes infiltration and perforin expression elevation of colon cancer in animal mode

Induksi Polifenol Mahkota Dewa dan Apoptosis Sel Kanker Paru Mencit Strain Balb/C: Analisis pada Up-Regulation Bax dan Down-Regulation Bcl-2

Mahkota Dewa polyphenol induction and apoptosis cell lung cancer in Balb/c strain mice analysis on up-regulation bax and downregulation Bcl-2Background: This research is aimed to prove the role of Mahkota Dewa polyphenol in the up-regulation Bax and down-regulation in strain Balb/c mice which inducted with benzo(a)pyrene (BaP).Method: Post test control group design with 30 strain Balb/c mice sample, aged 1-2 weeks, weighed 20-30 grams, healthy mice condition. All mice inducted with BaP and then randomized into 2 groups, which were the control group and the treatment group (polyphenol 50mg). The development of the lung carcinogenesis was observed by tissue surgery in the 8th, 17th and 26th week. The data collected include expression examination Bax and Bcl-2. The data analysis was conducted by Kruskal-Wallis, Mann-Whitneyand correlation test of Spearman's with significance degree of p<a (0.05).Result: The oral administration of Mahkota Dewa polyphenol of 50 mg were significantly decreased the occurance of lung carcinogenesis through decreasing of protein Bax and increasing Bcl-2 in treatment group in week 8, 17 and 26 (p=0.000). Protein Bax for the control group for week 8 were 4.04±0.22 and 1.92±0.10 in week 26, while the treatment group were 5.96±0.32 and 4.68±0.22 (p=0.000). Protein Bcl-2 for the control group for week 8 were 5.80±0.32 and 9.64±0.26 in week 26, while the treatmentgroup were 5.12±0.22 and 7.38±0.21 (p=0.000). The Spearman correlation for Bax and Bcl-2 with significancy value of (p=0.000).Conclusion: The administration of Mahkota Dewa polyphenol of 50 mg effectively increased Bax protein expression and decreased Bcl-2 protein expression in mice Balb/c

Ekstrak Phyllanthus Niruri Linn, Pertumbuhan Tumor dan Proliferasi Sel Kanker Kolorektal: Studi Eksperimental pada Tikus Sprague-Dawley yang Diinduksi 1,2 DMH

Effect of Phyllanthus niruri Linn extract on tumor growth and cell proliferation of colorectal cancer: experimental study in 1.2 DMH-induced Sprague-Dawley ratsBackground: The development of chemotherapy for colorectal cancer (CRC) is so very advanced, however, the survival of patients has not been satisfactory, therefore, the current therapy also involve immunotherapy. Phyllanthus niruri Linn (P. niruri L) may act as an immunomodulator and anticancer, but its potency has not been revealed. Study was conducted to confirm the effects of P. niruri L extract in the treatment of CRC.Method: The study was randomized posttest-only control group design. Samples were Sprague-Dawley male rats, bodyweight 170- 220 gr, were divided into two groups: non induced or negative control (K-) consisted of 9 normal rats, and induced by 1.2 DMH 30 mg/kgBW subcutaneously group consisted 30 rats. On the weeks 9, 11 and 13, four induced rats each week were sacrified to detect the development of CRC. On the weeks of 13th all of 4 rats were developed CRC, so the induction were stopped. The rest of 18 induced rats were randomly into two groups: without P. niruri L or positive control (K+)=9 rats and given P. niruri L extract 13.5 mg/kg orally or X group=9 rats. After 19th week all of rats were then terminated and tumor lesion of colon were examined macroscopically and hystophatologic tissues were stained with AgNORs for evaluate the cells proliferation. Oneway Anova and Post Hoc LSD test for the growth of colon tumor and non pairs t-test for cell proliferation were used. Considered significant if p was <0.05. Result: There was no tumor growth on K- group, while for K+ was 83.33±14.34% and on X was 40.44±13.23% (p=0.000). The mean of AgNORs on K+ was 4.60±0.55 while on X was 2.25±0.39 (p=0.000).Conclusion: The extract of P. niruri L suppress the tumor growth and cell proliferation of CRC

Perhitungan Kemauan Untuk Membayar Pada Penetapan Nilai Keselamatan Transportasi Jalan Untuk Kelompok Pengguna Jalan Tertentu

Some research about the value of road safety has been widely practiced in many countries. One approach that is commonly used for the determination of the value of the safety of road transport is by asking directly to the public about the preferences or willingness to pay in order to improve the safety aspects. Human preferences in this model are built on the behavioral theory with economics perspective, which explains that human behavior is formed by preference. The basic premise of this theory states that every human being has a preference for a number of goods. The problem is that safety is included in the category of non-market goods that are not traded and often rrespondents refuse to answer questions directly related to willingness to pay. Therefore, in order to obtain objective answers from the respondents, the form of questions for the willingness to pay should be linked with other proxies. The purpose of this study was to calculate the willingness to pay on valuation of road safety for specific communities by designing the willingness to pay questions which is associated with the proxies of vehicle maintenance and health maintenance

Face Validation Method Alternatives for Shiphandling Fuzzy Logic Difficulty Model

The development of shiphandling difficulty model for ferry is based on the empirical experience through the Master of Ro-Ro ferries. The SHDMF is consisted from two parts which are the Analytic Hierarchy Process (AHP) and Fuzzy Inference System. Both parts had been validated through internal validation in the form of consistency test for the first part and robustness test for the second part. Further, the external/face validation is required to compare the proposed model with similar model through benchmarking approach. The benchmarking approaches are elaborated for the reliability, validity, possibility, efficiency and effectiveness. Through fuzzy group decision making method, the questionnaire survey is performed to verify the most appropriate approach based on the shiphandling simulator as the most preferred benchmarking tool by experts. Next, the proposed scenario is overviewed and discussed especially related to the advantages and drawbacks of shiphandling simulator. Keywords: shiphandling difficulty, fuzzy group decision making, internal validation Model pengukuran kesulitan pengendalian feri didasarkan pada pengalaman empiris melalui pernyataan nahkoda kapal feri Ro-Ro. SHDMF terdiri atas dua bagian, yaitu Analytic Hierarchy Process dan Fuzzy Inference System. Kedua bagian ini telah divalidasi melalui validasi internal dalam bentuk uji konsistensi untuk bagian pertama dan uji kehandalan untuk bagian kedua. Selanjutnya validasi atau wajah eksternal diperlukan untuk membandingkan model yang diusulkan dengan model yang diperoleh dari benchmarking. Pendekatan benchmarking dijabarkan untuk kehandalan, validitas, kemungkinan, efisiensi, dan efektivitas. Melalui metode fuzzy kelompok pembuatan keputusan, survei kuesioner dilakukan untuk memverifikasi pendekatan yang paling tepat dengan simulator pengendalian kapal sebagai alat yang paling disukai oleh para ahli untuk benchmarking. Selanjutnya skenario yang ditinjau-ulang dan dibahas terutama terkait dengan keuntungan dan kelemahan simulator pengendalian kapal. Kata

Persepsi Pengguna Transjakarta Terhadap Aspek Amenity Pada Fasilitas Pejalan Kaki Di Halte Dukuh Atas Dengan Pendekatan Teori Perilaku Terencana

In addition to reliability and performance, another factors that affect the interest to use public transportation are convenience and aesthetics. Unlike the technical aspects that can be assessed by standard parameters, assessment of the convenience and aesthetics is a personal perception that may vary for each individual. The purpose of this research is to review the general perception of TransJakarta users on aspects that are related to amenities such as convenience and aesthetics. The scope of the review is pedestrian facilities on TransJakarta Dukuh Atas Shelter based on the approach of Theory of Planned Behaviour. The survey shows that majority of users considered that pedestrian facilities on Dukuh Atas Shelter are less comfortable and didn't have good aesthetic. The analysis shows that cleanliness, crowded and lighting condition are affecting users' intention in choosing TransJakarta as mode of choice