8 research outputs found
Furaquinocins K and L : Novel Naphthoquinone-Based Meroterpenoids from Streptomyces sp. Je 1-369
Actinomycetes are the most prominent group of microorganisms that produce biologically
active compounds. Among them, special attention is focused on bacteria in the genus Streptomyces.
Streptomycetes are an important source of biologically active natural compounds that could be
considered therapeutic agents. In this study, we described the identification, purification, and
structure elucidation of two new naphthoquinone-based meroterpenoids, furaquinocins K and L,
from Streptomyces sp. Je 1-369 strain, which was isolated from the rhizosphere soil of Juniperus excelsa
(Bieb.). The main difference between furaquinocins K and L and the described furaquinocins was a
modification in the polyketide naphthoquinone skeleton. In addition, the structure of furaquinocin
L contained an acetylhydrazone fragment, which is quite rare for natural compounds. We also
identified a furaquinocin biosynthetic gene cluster in the Je 1-369 strain, which showed similarity
(60%) with the furaquinocin B biosynthetic gene cluster from Streptomyces sp. KO-3988. Furaquinocin
L showed activity against Gram-positive bacteria without cytotoxic effects
Flavacol and Its Novel Derivative 3-Ξ²-Hydroxy Flavacol from Streptomyces sp. Pv 4-95 after the Expression of Heterologous AdpA
Actinomycetes are one of the main producers of biologically active compounds. However,
their capabilities have not been fully evaluated due to the presence of many unexpressed silent
clusters; moreover, actinomycetes can probably produce new or previously discovered natural
products under certain conditions. Overexpressing the adpA gene into streptomycetes strains can
unlock silent biosynthetic gene clusters. Herein, we showed that by applying this approach to
Streptomyces sp. Pv 4-95 isolated from Phyllostachys viridiglaucescens rhizosphere soil, two new
mass peaks were identified. NMR structure analysis identified these compounds as flavacol and
a new 3-Ξ²-hydroxy flavacol derivative. We suggest that the presence of heterologous AdpA has
no direct effect on the synthesis of flavacol and its derivatives in the Pv 4-95 strain. However,
AdpA affects the synthesis of precursors by increasing their quantity, which then condenses into the
resulting compounds
ΠΡΠΎΡΠΈΠ½ΡΠ΅ΡΠΈΡΠ½ΠΈΠΉ ΠΏΠΎΡΠ΅ΡΡΠ°Π» Π°ΠΊΡΠΈΠ½ΠΎΠΌΡΡΠ΅ΡΡΠ² ΡΠΈΠ·ΠΎΡΡΠ΅ΡΠΈ Helianthemum stevenii Rupr. Ex Juz. & Pozd.
Background. Today the actual problems of humanity are the high frequency of the emergence of new multi-resistant pathogenic microorganisms, lack of food and pollution, especially agro-ecosystems. Screening of new natural products and introducing them in medicine, veterinary medicine, agriculture, etc. is one of the approaches to solving these problems. Objects of particular interest are actinomycetes β record holders in the production of biologically active compounds.Objective. The purpose of the paper is evaluation of the biosynthetic properties of actinomycetes from the rhizosphere of the endemic Helianthemum stevenii Rupr. Ex Juz & Pozd. from Crimean peninsula.Methods. Samples of the H. stevenii Rupr. Ex Juz & Pozd. rhizosphere were taken on the southern slopes of the Nikita Range (Crimean Peninsula). Actinomycetes were isolated by direct sowing of the washed rhizosphere, aqua solution of phenol treatment of the roots, or by heating them at 100 Β°C within 60 minutes and sowing on the nutrient medium. Antibacterial and anti-candidiasis activities were studied by culturing the actinomycetes strains by a prick on the oat medium and pouring the 0.7% agar with a certain test culture. Antifungal properties were studied by putting the agar block with a 5-day fungal culture on cups with 3-day cultures of actinomycetes and subsequent incubation for 4 days. An activity index (AI) was determined by the ratio of the diameter zone of the inhibit growth test-cultures to the diameter of the actinomycetes colonies. Phytostimulants and enzymatic properties were studied by commonly accepted methods.Results. 107 actinomycetes strains from H. stevenii Rupr. Ex Juz. & Pozd. rhizosphere were isolated. 23.2% of isolates inhibited growth of one or more typical microbial test cultures which are capable of causing a disease in humans. 8.4% of the actinomycetes strains were antagonists of S. Π°ureus, the less actinomycetes strains inhibited gram-negative test cultures. AI of these strains was no more than 3. 80.1% of isolates were antagonists of at least one strain of the phytopathogenic bacteria. AI of these strains was from 1.2 to 13. Antifungal activity was in the 15.9% of isolates and their AI was no more than 3. 17.8 of actinomycetes strains were capable of producing indole-3-acetic acid, 10.3% β siderophores and 14.2% β phosphate solubilizers. A significant proportion of actinomycetes strains was capable of producing hydrolytic enzymes: amylases, lipases, pectinases and proteases. 29.9% of isolates can discolour Azure B and 8.4% are potentially laccase producers.Conclusions. The ability of actinomycetes strains from H. stevenii Rupr. Ex Juz. & Pozd. rhizosphere to produce antimicrobial compounds, enzymes and plants growth promoting (PGP) compounds was evaluated. Actinomycetes strains with wide range and specific inhibit certain pathogenic strain were selected as well as strains that specifically inhibit the growth of a particular strain of pathogenic Β microorganisms. A number of phytopathogenic bacteria antagonists tend to phytostimulation. The producers of hydrolases and oxidoreductase were isolated.ΠΡΠΎΠ±Π»Π΅ΠΌΠ°ΡΠΈΠΊΠ°. ΠΠΊΡΡΠ°Π»ΡΠ½ΡΠΌΠΈ ΠΏΡΠΎΠ±Π»Π΅ΠΌΠ°ΠΌΠΈ ΡΠ΅Π»ΠΎΠ²Π΅ΡΠ°ΡΡΠ²Π° ΡΠ΅Π³ΠΎΠ΄Π½Ρ ΡΠ²Π»ΡΡΡΡΡ Π²ΡΡΠΎΠΊΠ°Ρ ΡΠ°ΡΡΠΎΡΠ° Π²ΠΎΠ·Π½ΠΈΠΊΠ½ΠΎΠ²Π΅Π½ΠΈΡ Π½ΠΎΠ²ΡΡ
ΠΌΡΠ»ΡΡΠΈΡΠ΅Π·ΠΈΡΡΠ΅Π½ΡΠ½ΡΡ
ΡΠΎΡΠΌ ΠΏΠ°ΡΠΎΠ³Π΅Π½Π½ΡΡ
ΠΌΠΈΠΊΡΠΎΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠΎΠ², Π½Π΅Ρ
Π²Π°ΡΠΊΠ° ΠΏΡΠΎΠ΄ΡΠΊΡΠΎΠ² ΠΏΠΈΡΠ°Π½ΠΈΡ, Π° ΡΠ°ΠΊΠΆΠ΅ Π·Π°Π³ΡΡΠ·Π½Π΅Π½ΠΈΠ΅ ΠΎΠΊΡΡΠΆΠ°ΡΡΠ΅ΠΉ ΡΡΠ΅Π΄Ρ, ΠΎΡΠΎΠ±Π΅Π½Π½ΠΎ Π°Π³ΡΠΎΡΠΊΠΎΡΠΈΡΠ΅ΠΌ. CΠΊΡΠΈΠ½ΠΈΠ½Π³ Π½ΠΎΠ²ΡΡ
ΠΏΡΠΈΡΠΎΠ΄Π½ΡΡ
ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΠΉ ΠΈ Π²Π½Π΅Π΄ΡΠ΅Π½ΠΈΠ΅ ΠΈΡ
Π² ΠΌΠ΅Π΄ΠΈΡΠΈΠ½Π΅, Π²Π΅ΡΠ΅ΡΠΈΠ½Π°ΡΠΈΠΈ, ΡΠ΅Π»ΡΡΠΊΠΎΠΌ Ρ
ΠΎΠ·ΡΠΉΡΡΠ²Π΅ ΠΈ Π΄ΡΡΠ³ΠΈΡ
ΠΎΡΡΠ°ΡΠ»ΡΡ
ΡΠ²Π»ΡΠ΅ΡΡΡ ΠΎΠ΄Π½ΠΈΠΌ ΠΈΠ· ΠΏΠΎΠ΄Ρ
ΠΎΠ΄ΠΎΠ² ΠΊ ΡΠ΅ΡΠ΅Π½ΠΈΡ ΡΡΠΈΡ
ΠΏΡΠΎΠ±Π»Π΅ΠΌ. ΠΡΠΎΠ±Π΅Π½Π½ΡΠΉ ΠΈΠ½ΡΠ΅ΡΠ΅Ρ Π²ΡΠ·ΡΠ²Π°ΡΡ Π°ΠΊΡΠΈΠ½ΠΎΠΌΠΈΡΠ΅ΡΡ β ΡΠ΅ΠΊΠΎΡΠ΄ΡΠΌΠ΅Π½Ρ ΠΏΠΎ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Ρ ΡΠΈΠ½ΡΠ΅Π·ΠΈΡΡΠ΅ΠΌΡΡ
Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈ Π°ΠΊΡΠΈΠ²Π½ΡΡ
ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΠΉ.Π¦Π΅Π»Ρ. Π¦Π΅Π»Ρ ΡΠ°Π±ΠΎΡΡ ΡΠΎΡΡΠΎΠΈΡ Π² ΠΎΡΠ΅Π½ΠΊΠ΅ Π±ΠΈΠΎΡΠΈΠ½ΡΠ΅ΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΠ²ΠΎΠΉΡΡΠ² Π°ΠΊΡΠΈΠ½ΠΎΠΌΠΈΡΠ΅ΡΠΎΠ² ΡΠΈΠ·ΠΎΡΡΠ΅ΡΡ ΡΠ½Π΄Π΅ΠΌΠΈΠΊΠ° ΠΡΡΠΌΡΠΊΠΎΠ³ΠΎ ΠΏΠΎΠ»ΡΠΎΡΡΡΠΎΠ²Π° ΡΠΎΠ»Π½ΡΠ΅ΡΠ²Π΅ΡΠ° Π‘ΡΠ΅Π²Π΅Π½Π° Helianthemum stevenii Rupr. Ex Juz. & Pozd.ΠΠ΅ΡΠΎΠ΄ΠΈΠΊΠ° ΡΠ΅Π°Π»ΠΈΠ·Π°ΡΠΈΠΈ. ΠΠ±ΡΠ°Π·ΡΡ ΡΠΈΠ·ΠΎΡΡΠ΅ΡΡ H. stevenii Rupr. Ex Juz. & Pozd. ΠΎΡΠ±ΠΈΡΠ°Π»ΠΈ Π½Π° ΡΠΆΠ½ΡΡ
ΡΠΊΠ»ΠΎΠ½Π°Ρ
ΠΠΈΠΊΠΈΡΡΠΊΠΎΠ³ΠΎ Ρ
ΡΠ΅Π±ΡΠ° (ΠΡΡΠΌΡΠΊΠΈΠΉ ΠΏΠΎΠ»ΡΠΎΡΡΡΠΎΠ²). ΠΠ·ΠΎΠ»ΠΈΡΠΎΠ²Π°Π»ΠΈ Π°ΠΊΡΠΈΠ½ΠΎΠΌΠΈΡΠ΅ΡΡ ΠΏΡΡΠ΅ΠΌ ΠΏΡΡΠΌΠΎΠ³ΠΎ ΠΏΠΎΡΠ΅Π²Π° ΡΠΌΡΠ²ΠΎΠ² ΡΠΈΠ·ΠΎΡΡΠ΅ΡΡ, ΠΎΠ±ΡΠ°Π±ΠΎΡΠΊΠΈ ΠΊΠΎΡΠ½Π΅ΠΉ Π²ΠΎΠ΄Π½ΡΠΌ ΡΠ°ΡΡΠ²ΠΎΡΠΎΠΌ ΡΠ΅Π½ΠΎΠ»Π° ΠΈΠ»ΠΈ ΠΏΡΠΎΠ³ΡΠ΅Π²Π°Π½ΠΈΡ ΠΈΡ
ΠΏΡΠΈ 100 Β°Π‘ Π½Π° ΠΏΡΠΎΡΡΠΆΠ΅Π½ΠΈΠΈ 60 ΠΌΠΈΠ½ Ρ ΠΏΠΎΡΠ»Π΅Π΄ΡΡΡΠΈΠΌ ΠΏΠΎΡΠ΅Π²ΠΎΠΌ ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΡΡ
ΡΡΡΠΏΠ΅Π½Π·ΠΈΠΉ Π½Π° ΠΏΠΈΡΠ°ΡΠ΅Π»ΡΠ½ΡΠ΅ ΡΡΠ΅Π΄Ρ. ΠΠ½ΡΠΈΠ±Π°ΠΊΡΠ΅ΡΠΈΠ°Π»ΡΠ½ΡΠ΅ ΠΈ ΠΏΡΠΎΡΠΈΠ²ΠΎΠΊΠ°Π½Π΄ΠΈΠ΄ΠΎΠ·Π½ΡΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΈΠ·ΡΡΠ°Π»ΠΈ ΠΏΡΡΠ΅ΠΌ ΠΏΠΎΡΠ΅Π²Π° ΠΊΡΠ»ΡΡΡΡ Π°ΠΊΡΠΈΠ½ΠΎΠΌΠΈΡΠ΅ΡΠΎΠ² ΡΠΊΠΎΠ»ΠΎΠΌ Π½Π° ΠΎΠ²ΡΡΠ½ΡΠΉ Π°Π³Π°Ρ ΠΈ Π·Π°Π»ΠΈΠ²ΠΊΠΈ 0,7 %-Π½ΡΠΌ Π°Π³Π°ΡΠΎΠΌ Ρ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½Π½ΠΎΠΉ ΡΠ΅ΡΡ-ΠΊΡΠ»ΡΡΡΡΠΎΠΉ. ΠΠ½ΡΠΈΡΡΠ½Π³Π°Π»ΡΠ½ΡΠ΅ ΡΠ²ΠΎΠΉΡΡΠ²Π° ΠΈΠ·ΡΡΠ°Π»ΠΈ ΠΏΡΡΠ΅ΠΌ Π²ΡΠΊΠ»Π°Π΄ΡΠ²Π°Π½ΠΈΡ Π°Π³Π°ΡΠΎΠ²ΠΎΠ³ΠΎ Π±Π»ΠΎΠΊΠ° Ρ 5-Π΄Π½Π΅Π²Π½ΠΎΠΉ ΠΊΡΠ»ΡΡΡΡΠΎΠΉ Π³ΡΠΈΠ±Π° Π½Π° ΡΠ°ΡΠΊΠΈ Ρ 3-Π΄Π½Π΅Π²Π½ΡΠΌΠΈ ΠΊΡΠ»ΡΡΡΡΠ°ΠΌΠΈ Π°ΠΊΡΠΈΠ½ΠΎΠΌΠΈΡΠ΅ΡΠΎΠ² ΠΈ ΠΏΠΎΡΠ»Π΅Π΄ΡΡΡΠΈΠΌ ΠΈΠ½ΠΊΡΠ±ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ Π½Π° ΠΏΡΠΎΡΡΠΆΠ΅Π½ΠΈΠΈ 4 ΡΡΡΠΎΠΊ. ΠΠΎ ΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΡ Π΄ΠΈΠ°ΠΌΠ΅ΡΡΠ° Π·ΠΎΠ½ ΡΠ³Π½Π΅ΡΠ΅Π½ΠΈΡ ΡΠΎΡΡΠ° ΡΠ΅ΡΡ-ΠΊΡΠ»ΡΡΡΡ ΠΊ Π΄ΠΈΠ°ΠΌΠ΅ΡΡΡ ΠΊΠΎΠ»ΠΎΠ½ΠΈΠΉ Π°ΠΊΡΠΈΠ½ΠΎΠΌΠΈΡΠ΅ΡΠΎΠ² ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ»ΠΈ ΠΈΠ½Π΄Π΅ΠΊΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ (ΠΠ). Π€ΠΈΡΠΎΡΡΠΈΠΌΡΠ»ΠΈΡΡΡΡΠΈΠ΅ ΠΈ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ°ΡΠΈΠ²Π½ΡΠ΅ ΡΠ²ΠΎΠΉΡΡΠ²Π° ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ»ΠΈ ΠΎΠ±ΡΠ΅ΠΏΡΠΈΠ½ΡΡΡΠΌΠΈ ΠΌΠ΅ΡΠΎΠ΄Π°ΠΌΠΈ.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. ΠΠ· ΡΠΈΠ·ΠΎΡΡΠ΅ΡΡ H. stevenii Rupr. Ex Juz. & Pozd. ΠΈΠ·ΠΎΠ»ΠΈΡΠΎΠ²Π°Π½Ρ 107 ΡΡΠ°ΠΌΠΌΠΎΠ² Π°ΠΊΡΠΈΠ½ΠΎΠΌΠΈΡΠ΅ΡΠΎΠ². 23,2Β % ΠΈΠ·ΠΎΠ»ΡΡΠΎΠ² ΡΠ³Π½Π΅ΡΠ°Π»ΠΈ ΡΠΎΡΡ ΠΎΠ΄Π½ΠΎΠ³ΠΎ ΠΈ Π±ΠΎΠ»Π΅Π΅ ΡΠΈΠΏΠΈΡΠ½ΡΡ
ΡΡΠ°ΠΌΠΌΠΎΠ² ΠΌΠΈΠΊΡΠΎΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠΎΠ², ΡΠΏΠΎΡΠΎΠ±Π½ΡΡ
Π²ΡΠ·ΡΠ²Π°ΡΡ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΈ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°. 8,4Β % ΠΈΠ·ΠΎΒΠ»ΡΡΠΎΠ² Π±ΡΠ»ΠΈ Π°Π½ΡΠ°Π³ΠΎΠ½ΠΈΡΡΠ°ΠΌΠΈ S.Β Π°ureus, ΡΠΎΡΡ Π³ΡΠ°ΠΌΠΎΡΡΠΈΡΠ°ΡΠ΅Π»ΡΠ½ΡΡ
Π±Π°ΠΊΡΠ΅ΡΠΈΠΉ Π·Π°Π΄Π΅ΡΠΆΠΈΠ²Π°Π»ΠΎ ΡΡΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎ ΠΌΠ΅Π½ΡΡΠ΅ ΡΡΠ°ΠΌΠΌΠΎΠ² Π°ΠΊΡΠΈΠ½ΠΎΠΌΠΈΡΠ΅ΡΠΎΠ². ΠΠ ΡΡΠΈΡ
ΠΈΠ·ΠΎΠ»ΡΡΠΎΠ² Π½Π΅ ΠΏΡΠΈΠ²ΡΡΠ°Π» 3. 80,1 % ΠΈΠ·ΠΎΠ»ΡΡΠΎΠ² Π±ΡΠ»ΠΈ Π°Π½ΡΠ°Π³ΠΎΠ½ΠΈΡΡΠ°ΠΌΠΈ Ρ
ΠΎΡΡ Π±Ρ ΠΎΠ΄Π½ΠΎΠ³ΠΎ ΡΡΠ°ΠΌΠΌΠ° ΡΠΈΡΠΎΠΏΠ°ΡΠΎΠ³Π΅Π½Π½ΡΡ
Π±Π°ΠΊΡΠ΅ΡΠΈΠΉ. ΠΠ ΡΡΠΈΡ
ΡΡΠ°ΠΌΠΌΠΎΠ² ΡΠΎΡΡΠ°Π²Π»ΡΠ» 1,2β13,0. ΠΠ½ΡΠΈΡΡΠ½Π³Π°Π»ΡΠ½ΡΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΠΏΡΠΎΡΠ²Π»ΡΠ»ΠΈ 15,9 % ΡΡΠ°ΠΌΠΌΠΎΠ² Π°ΠΊΡΠΈΠ½ΠΎΠΌΠΈΡΠ΅ΡΠΎΠ², Π° ΠΈΡ
ΠΠ Π½Π΅ ΠΏΡΠ΅Π²ΡΡΠ°Π» 3. 17,8 % ΠΈΠ·ΠΎΠ»ΡΡΠΎΠ² ΠΏΡΠΎΠ΄ΡΡΠΈΡΠΎΠ²Π°Π»ΠΈ ΠΈΠ½Π΄ΠΎΠ»ΠΈΠ»-3-ΡΠΊΡΡΡΠ½ΡΡ ΠΊΠΈΡΠ»ΠΎΡΡ, 10,3 % β ΡΠΈΠ΄Π΅ΡΠΎΡΠΎΡΡ, Π° 14,2Β % β ΡΠΎΠ»ΡΠ±ΠΈΠ»ΠΈΠ·ΠΈΡΠΎΠ²Π°Π»ΠΈ ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΡ ΡΠΎΡΡΠΎΡΠ°. ΠΡΠ°ΡΠΈΡΠ΅Π»ΡΠ½Π°Ρ ΡΠ°ΡΡΡ ΡΡΠ°ΠΌΠΌΠΎΠ² Π°ΠΊΡΠΈΠ½ΠΎΠΌΠΈΡΠ΅ΡΠΎΠ² ΠΏΡΠΎΠ΄ΡΡΠΈΡΠΎΠ²Π°Π»Π° Π³ΠΈΠ΄ΡΠΎΠ»ΠΈΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΡΠ΅ΡΠΌΠ΅Π½ΡΡ: Π°ΠΌΠΈΠ»Π°Π·Ρ, Π»ΠΈΠΏΠ°Π·Ρ, ΡΠ΅Π»Π»ΡΠ»Π°Π·Ρ, ΠΏΡΠΎΡΠ΅Π°Π·Ρ. 29,9 % ΠΈΠ·ΠΎΠ»ΡΡΠΎΠ² Π΄Π΅ΠΊΠΎΠ»ΠΎΡΠΈΠ·ΠΈΡΠΎΠ²Π°Π»ΠΈ Azure B, Π° ΡΠ°ΠΊΠΆΠ΅ 8,4Β % ΡΡΠ°ΠΌΠΌΠΎΠ² Π°ΠΊΡΠΈΠ½ΠΎΠΌΠΈΡΠ΅ΡΠΎΠ² ΡΠΈΠ½ΡΠ΅Π·ΠΈΡΠΎΠ²Π°Π»ΠΈ Π»Π°ΠΊΠΊΠ°Π·Ρ.ΠΡΠ²ΠΎΠ΄Ρ. ΠΡΠ΅Π½Π΅Π½Π° ΡΠΏΠΎΡΠΎΠ±Π½ΠΎΡΡΡ Π°ΠΊΡΠΈΠ½ΠΎΠΌΠΈΡΠ΅ΡΠΎΠ² ΡΠΈΠ·ΠΎΡΡΠ΅ΡΡ H. stevenii Rupr. Ex Juz. & Pozd ΡΠΈΠ½ΡΠ΅Π·ΠΈΡΠΎΠ²Π°ΡΡ ΠΏΡΠΎΡΠΈΠ²ΠΎΠΌΠΈΠΊΡΠΎΠ±Π½ΡΠ΅ ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΡ, ΡΠ΅ΡΠΌΠ΅Π½ΡΡ, Π° ΡΠ°ΠΊΠΆΠ΅ ΠΌΠΎΠ»Π΅ΠΊΡΠ»Ρ, ΡΠΏΠΎΡΠΎΠ±Π½ΡΠ΅ ΡΡΠΈΠΌΡΠ»ΠΈΡΠΎΠ²Π°ΡΡ ΡΠΎΡΡ ΠΈ ΡΠ°Π·Π²ΠΈΡΠΈΠ΅ ΡΠ°ΡΡΠ΅Π½ΠΈΠΉ. ΠΠ·ΠΎΠ»ΠΈΡΠΎΠ²Π°Π½Ρ ΡΡΠ°ΠΌΠΌΡ Π°ΠΊΡΠΈΠ½ΠΎΠΌΠΈΡΠ΅ΡΠΎΠ² Ρ ΡΠΈΡΠΎΠΊΠΈΠΌ ΡΠΏΠ΅ΠΊΡΡΠΎΠΌ Π°Π½ΡΠΈΠΌΠΈΠΊΡΠΎΠ±Π½ΠΎΠ³ΠΎ Π΄Π΅ΠΉΡΡΠ²ΠΈΡ, Π° ΡΠ°ΠΊΠΆΠ΅ ΡΡΠ°ΠΌΠΌΡ, ΠΊΠΎΡΠΎΡΡΠ΅ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΈ ΡΠ³Π½Π΅ΡΠ°ΡΡ ΡΠΎΡΡ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½Π½ΠΎΠ³ΠΎ ΡΡΠ°ΠΌΠΌΠ° ΠΏΠ°ΡΠΎΠ³Π΅Π½Π½ΡΡ
ΠΌΠΈΠΊΡΠΎΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠΎΠ². ΠΠ»Ρ ΡΡΠ΄Π° Π°Π½ΡΠ°Π³ΠΎΠ½ΠΈΡΡΠΎΠ² ΡΠΈΡΠΎΠΏΠ°ΡΠΎΠ³Π΅Π½Π½ΠΎΠΉ ΠΌΠΈΠΊΡΠΎΡΠ»ΠΎΡΡ ΡΠ²ΠΎΠΉΡΡΠ²Π΅Π½Π½ΠΎ ΡΠΈΡΠΎΡΡΠΈΠΌΡΠ»ΠΈΡΡΡΡΠ΅Π΅ Π΄Π΅ΠΉΡΡΠ²ΠΈΠ΅. ΠΠ·ΠΎΠ»ΠΈΡΠΎΠ²Π°Π½Ρ ΠΏΡΠΎΠ΄ΡΡΠ΅Π½ΡΡ Π³ΠΈΠ΄ΡΠΎΠ»Π°Π· ΠΈ ΠΎΠΊΡΠΈΠ΄ΠΎΡΠ΅Π΄ΡΠΊΡΠ°Π·.ΠΡΠΎΠ±Π»Π΅ΠΌΠ°ΡΠΈΠΊΠ°. ΠΠΊΡΡΠ°Π»ΡΠ½ΠΈΠΌΠΈ ΠΏΡΠΎΠ±Π»Π΅ΠΌΠ°ΠΌΠΈ Π»ΡΠ΄ΡΡΠ²Π° ΡΡΠΎΠ³ΠΎΠ΄Π½Ρ Ρ Π²ΠΈΡΠΎΠΊΠ° ΡΠ°ΡΡΠΎΡΠ° Π²ΠΈΠ½ΠΈΠΊΠ½Π΅Π½Π½Ρ Π½ΠΎΠ²ΠΈΡ
ΠΌΡΠ»ΡΡΠΈΒΡΠ΅Π·ΠΈΡΡΠ΅Π½ΡΠ½ΠΈΡ
ΡΠΎΡΠΌ ΠΏΠ°ΡΠΎΠ³Π΅Π½Π½ΠΈΡ
ΠΌΡΠΊΡΠΎΠΎΡΠ³Π°Π½ΡΠ·ΠΌΡΠ², Π½Π΅ΡΡΠ°ΡΠ° ΠΏΡΠΎΠ΄ΡΠΊΡΡΠ² Ρ
Π°ΡΡΡΠ²Π°Π½Π½Ρ, Π° ΡΠ°ΠΊΠΎΠΆ Π·Π°Π±ΡΡΠ΄Π½Π΅Π½Π½Ρ Π½Π°Π²ΠΊΠΎΠ»ΠΈΡΠ½ΡΠΎΠ³ΠΎ ΡΠ΅ΡΠ΅Π΄ΠΎΠ²ΠΈΡΠ°, ΠΎΡΠΎΠ±Π»ΠΈΠ²ΠΎ Π°Π³ΡΠΎΠ΅ΠΊΠΎΡΠΈΡΠ΅ΠΌ. Π‘ΠΊΡΠΈΠ½ΡΠ½Π³ Π½ΠΎΠ²ΠΈΡ
ΠΏΡΠΈΡΠΎΠ΄Π½ΠΈΡ
ΠΏΡΠΎΠ΄ΡΠΊΡΡΠ² ΡΠ° Π²ΠΏΡΠΎΠ²Π°Π΄ΠΆΠ΅Π½Π½Ρ ΡΡ
Ρ ΠΌΠ΅Π΄ΠΈΡΠΈΠ½Ρ, Π²Π΅ΡΠ΅ΡΠΈΠ½Π°ΡΡΡ, ΡΡΠ»ΡΡΡΠΊΠΎΠΌΡ Π³ΠΎΡΠΏΠΎΠ΄Π°ΡΡΡΠ²Ρ ΡΠΎΡΠΎ Ρ ΠΎΠ΄Π½ΠΈΠΌ ΡΠ· ΠΏΡΠ΄Ρ
ΠΎΠ΄ΡΠ² Π΄ΠΎ Π²ΠΈΡΡΡΠ΅Π½Π½Ρ ΡΠΈΡ
ΠΏΡΠΎΠ±Π»Π΅ΠΌ. ΠΡΠΎΠ±Π»ΠΈΠ²ΠΈΠΉ ΡΠ½ΡΠ΅ΡΠ΅Ρ Π²ΠΈΠΊΠ»ΠΈΠΊΠ°ΡΡΡ Π°ΠΊΡΠΈΠ½ΠΎΠΌΡΡΠ΅ΡΠΈ β ΡΠ΅ΠΊΠΎΡΠ΄ΡΠΌΠ΅Π½ΠΈ Π·Π° ΠΊΡΠ»ΡΠΊΡΡΡΡ ΡΠΈΠ½ΡΠ΅Π·ΠΎΠ²Π°Π½ΠΈΡ
Π±ΡΠΎΠ»ΠΎΠ³ΡΡΠ½ΠΎ Π°ΠΊΡΠΈΠ²Π½ΠΈΡ
ΡΠΏΠΎΠ»ΡΠΊ.ΠΠ΅ΡΠ°. ΠΠ΅ΡΠΎΡ ΡΠΎΠ±ΠΎΡΠΈ Ρ ΠΎΡΡΠ½ΠΊΠ° Π±ΡΠΎΡΠΈΠ½ΡΠ΅ΡΠΈΡΠ½ΠΈΡ
Π²Π»Π°ΡΡΠΈΠ²ΠΎΡΡΠ΅ΠΉ Π°ΠΊΡΠΈΠ½ΠΎΠΌΡΡΠ΅ΡΡΠ² ΡΠΈΠ·ΠΎΡΡΠ΅ΡΠΈ Π΅Π½Π΄Π΅ΠΌΡΠΊΠ° ΠΡΠΈΠΌΡΡΠΊΠΎΠ³ΠΎ ΠΏΡΠ²ΠΎΡΡΡΠΎΠ²Π° ΡΠΎΠ½ΡΠ½ΠΊΠΈ CΡΠ΅Π²Π΅Π½Π° Helianthemum stevenii Rupr. Ex Juz. & Pozd.ΠΠ΅ΡΠΎΠ΄ΠΈΠΊΠ° ΡΠ΅Π°Π»ΡΠ·Π°ΡΡΡ. ΠΡΠ°Π·ΠΊΠΈ ΡΠΈΠ·ΠΎΡΡΠ΅ΡΠΈ H. stevenii Rupr. Ex Juz. & Pozd. Π²ΡΠ΄Π±ΠΈΡΠ°Π»ΠΈ Π½Π° ΠΏΡΠ²Π΄Π΅Π½Π½ΠΈΡ
ΡΡ
ΠΈΠ»Π°Ρ
ΠΡΠΊΡΡΡΡΠΊΠΎΠ³ΠΎ Ρ
ΡΠ΅Π±ΡΠ° (ΠΡΠΈΠΌΡΡΠΊΠΈΠΉ ΠΏΡΠ²ΠΎΡΡΡΡΠ²). ΠΠΈΠ΄ΡΠ»ΡΠ»ΠΈ Π°ΠΊΡΠΈΠ½ΠΎΠΌΡΡΠ΅ΡΠΈ ΠΏΡΡΠΌΠΈΠΌ ΠΏΠΎΡΡΠ²ΠΎΠΌ Π·ΠΌΠΈΠ²ΡΠ² ΡΠΈΠ·ΠΎΡΡΠ΅ΡΠΈ, ΠΎΠ±ΡΠΎΠ±ΠΊΠΎΡ ΠΊΠΎΡΠ΅Π½ΡΠ² Π²ΠΎΠ΄Π½ΠΈΠΌ ΡΠΎΠ·ΡΠΈΠ½ΠΎΠΌ ΡΠ΅Π½ΠΎΠ»Ρ Π°Π±ΠΎ ΠΏΡΠΎΠΆΠ°ΡΡΠ²Π°Π½Π½ΡΠΌ ΡΡ
Π·Π° 100 Β°Π‘ ΠΏΡΠΎΡΡΠ³ΠΎΠΌ 60 Ρ
Π² Π· ΠΏΠΎΠ΄Π°Π»ΡΡΠΈΠΌ Π²ΠΈΡΡΠ²Π°Π½Π½ΡΠΌ Π½Π° ΠΆΠΈΠ²ΠΈΠ»ΡΠ½Ρ ΡΠ΅ΡΠ΅Π΄ΠΎΠ²ΠΈΡΠ°. ΠΠ½ΡΠΈΠ±Π°ΠΊΡΠ΅ΡΡΠΉΠ½Ρ ΡΠ° ΠΏΡΠΎΡΠΈΠΊΠ°Π½Π΄ΠΈΠ΄ΠΎΠ·Π½Ρ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ Π²ΠΈΠ²ΡΠ°Π»ΠΈ ΡΠ΅ΡΠ΅Π· Π²ΠΈΡΡΠ²Π°Π½Π½Ρ ΠΊΡΠ»ΡΡΡΡ Π°ΠΊΡΠΈΠ½ΠΎΠΌΡΡΠ΅ΡΡΠ² ΡΠΊΠΎΠ»ΠΎΠΌ Π½Π° Π²ΡΠ²ΡΡΠ½Π΅ ΡΠ΅ΡΠ΅Π΄ΠΎΠ²ΠΈΡΠ΅ Ρ Π·Π°Π»ΠΈΠ²Π°Π½Π½Ρ 0,7Β %-Π½ΠΈΠΌ Π°Π³Π°ΡΠΎΠΌ Π· ΠΏΠ΅Π²Π½ΠΎΡ ΡΠ΅ΡΡ-ΠΊΡΠ»ΡΡΡΡΠΎΡ. ΠΠ½ΡΠΈΡΡΠ½Π³Π°Π»ΡΠ½Ρ Π²Π»Π°ΡΡΠΈΠ²ΠΎΡΡΡ Π²ΠΈΠ²ΡΠ°Π»ΠΈ ΡΠ΅ΡΠ΅Π· Π²ΠΈΠΊΠ»Π°Π΄Π°Π½Π½Ρ Π°Π³Π°ΡΠΎΠ²ΠΎΠ³ΠΎ Π±Π»ΠΎΠΊΡ Π· 5-Π΄Π΅Π½Π½ΠΎΡ ΠΊΡΠ»ΡΡΡΡΠΎΡ Π³ΡΠΈΠ±Π° Π½Π° ΡΠ°ΡΠΊΠΈ Π· 3-Π΄Π΅Π½Π½ΠΈΠΌΠΈ ΠΊΡΠ»ΡΡΡΡΠ°ΠΌΠΈ Π°ΠΊΡΠΈΠ½ΠΎΠΌΡΡΠ΅ΡΡΠ² ΡΠ° ΠΏΠΎΠ΄Π°Π»ΡΡΠΈΠΌ ΡΠ½ΠΊΡΠ±ΡΠ²Π°Π½Π½ΡΠΌ ΠΏΡΠΎΡΡΠ³ΠΎΠΌ 4-Ρ
Π΄ΡΠ±. ΠΠ° Π²ΡΠ΄Π½ΠΎΡΠ΅Π½Π½ΡΠΌ Π΄ΡΠ°ΠΌΠ΅ΡΡΠ° Π·ΠΎΠ½ ΠΏΡΠΈΠ³Π½ΡΡΠ΅Π½Π½Ρ ΡΠΎΡΡΡ ΡΠ΅ΡΡ-ΠΊΡΠ»ΡΡΡΡ Π΄ΠΎ Π΄ΡΠ°ΠΌΠ΅ΡΡΠ° ΠΊΠΎΠ»ΠΎΠ½ΡΠΉ Π°ΠΊΡΠΈΠ½ΠΎΠΌΡΡΠ΅ΡΡΠ² Π²ΠΈΠ·Π½Π°ΡΠ°Π»ΠΈ ΡΠ½Π΄Π΅ΠΊΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ (ΠΠ). Π€ΡΡΠΎΡΡΠΈΠΌΡΠ»ΡΠ²Π°Π»ΡΠ½Ρ ΡΠ° ΡΠ΅ΡΒΠΌΠ΅Π½ΡΠ°ΡΠΈΠ²Π½Ρ Π²Π»Π°ΡΡΠΈΠ²ΠΎΡΡΡ Π²ΠΈΠ²ΡΠ°Π»ΠΈ Π·Π°Π³Π°Π»ΡΠ½ΠΎΠΏΡΠΈΠΉΠ½ΡΡΠΈΠΌΠΈ ΠΌΠ΅ΡΠΎΠ΄Π°ΠΌΠΈ.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΠΈ. Π ΡΠΈΠ·ΠΎΡΡΠ΅ΡΠΈ H. stevenii Rupr. Ex Juz. & Pozd. Π²ΠΈΠ΄ΡΠ»Π΅Π½ΠΎ 107 ΡΡΠ°ΠΌΡΠ² Π°ΠΊΡΠΈΠ½ΠΎΠΌΡΡΠ΅ΡΡΠ². 23,2Β % ΡΠ·ΠΎΠ»ΡΡΡΠ² ΠΏΡΠΈΠ³Π½ΡΡΡΠ²Π°Π»ΠΈ ΡΡΡΡ ΠΎΠ΄Π½ΠΎΠ³ΠΎ Ρ Π±ΡΠ»ΡΡΠ΅ ΡΠΈΠΏΠΎΠ²ΠΈΡ
ΡΡΠ°ΠΌΡΠ² ΠΌΡΠΊΡΠΎΠΎΡΠ³Π°Π½ΡΠ·ΠΌΡΠ², Π·Π΄Π°ΡΠ½ΠΈΡ
ΡΠΏΡΠΈΡΠΈΠ½ΡΠ²Π°ΡΠΈ ΡΠ½ΡΠ΅ΠΊΡΡΡ Π»ΡΠ΄ΠΈΠ½ΠΈ. 8,4 % ΡΡΠ°ΠΌΡΠ² Π°ΠΊΡΠΈΠ½ΠΎΠΌΡΡΠ΅ΡΡΠ² Π±ΡΠ»ΠΈ Π°Π½ΡΠ°Π³ΠΎΠ½ΡΡΡΠ°ΠΌΠΈ S. Π°ureus, ΡΡΡΡ Π³ΡΠ°ΠΌΠ½Π΅Π³Π°ΡΠΈΠ²Π½ΠΈΡ
Π±Π°ΠΊΡΠ΅ΡΡΠΉ Π·Π°ΡΡΠΈΠΌΡΠ²Π°Π»ΠΎ Π·Π½Π°ΡΠ½ΠΎ ΠΌΠ΅Π½ΡΠ΅ Π΄ΠΎΡΠ»ΡΠ΄ΠΆΡΠ²Π°Π½ΠΈΡ
ΠΊΡΠ»ΡΡΡΡ. ΠΠ ΡΠΈΡ
ΡΠ·ΠΎΠ»ΡΡΡΠ² Π½Π΅ ΠΏΠ΅ΡΠ΅Π²ΠΈΡΡΠ²Π°Π² 3. 80,1Β % ΡΠ·ΠΎΠ»ΡΡΡΠ² Π±ΡΠ»ΠΈ Π°Π½ΡΠ°Π³ΠΎΠ½ΡΡΡΠ°ΠΌΠΈ Ρ
ΠΎΡΠ° Π± ΠΎΠ΄Π½ΠΎΠ³ΠΎ ΡΡΠ°ΠΌΡ ΡΡΡΠΎΠΏΠ°ΡΠΎΠ³Π΅Π½Π½ΠΈΡ
Π±Π°ΠΊΡΠ΅ΡΡΠΉ. ΠΠ ΡΠΈΡ
ΡΡΠ°ΠΌΡΠ² ΡΡΠ°Π½ΠΎΠ²ΠΈΠ² 1,2β13,0. ΠΠ½ΡΠΈΡΡΠ½Π³Π°Π»ΡΠ½Ρ Π°ΠΊΡΠΈΠ²Π½ΡΡΡΡ Π²ΠΈΡΠ²Π»ΡΠ»ΠΎ 15,9Β % ΡΠ·ΠΎΠ»ΡΡΡΠ², Π° ΡΡ
ΠΠ Π½Π΅ ΠΏΠ΅ΡΠ΅Π²ΠΈΡΡΠ²Π°Π² 3. 17,8Β % ΡΡΠ°ΠΌΡΠ² ΠΏΡΠΎΠ΄ΡΠΊΡΠ²Π°Π»ΠΈ ΡΠ½Π΄ΠΎΠ»ΡΠ»-3-ΠΎΡΡΠΎΠ²Ρ ΠΊΠΈΡΠ»ΠΎΡΡ, 10,3Β % β ΡΠΈΠ΄Π΅ΡΠΎΡΠΎΡΠΈ, Π° 14,2Β % ΡΠΎΠ»ΡΠ±ΡΠ»ΡΠ·ΡΠ²Π°Π»ΠΈ ΡΠΏΠΎΠ»ΡΠΊΠΈ ΡΠΎΡΡΠΎΡΡ. ΠΠ½Π°ΡΠ½Π° ΡΠ°ΡΡΠΊΠ° ΡΡΠ°ΠΌΡΠ² Π°ΠΊΡΠΈΠ½ΠΎΠΌΡΡΠ΅ΡΡΠ² ΠΏΡΠΎΠ΄ΡΠΊΡΠ²Π°Π»ΠΈ Π³ΡΠ΄ΡΠΎΠ»ΡΡΠΈΡΠ½Ρ ΡΠ΅ΡΠΌΠ΅Π½ΡΠΈ: Π°ΠΌΡΠ»Π°Π·ΠΈ, Π»ΡΠΏΠ°Π·ΠΈ, ΠΏΠ΅ΠΊΡΠΈΠ½Π°Π·ΠΈ, ΠΏΡΠΎΡΠ΅Π°Π·ΠΈ. 29,9Β % ΡΠ·ΠΎΠ»ΡΡΡΠ² Π·Π΄Π°ΡΠ½Ρ Π΄Π΅ΠΊΠΎΠ»ΠΎΡΠΈΠ·ΡΠ²Π°ΡΠΈ Azure B, Π° ΡΠ°ΠΊΠΎΠΆ 8,4Β % ΡΡΠ°ΠΌΡΠ² Π°ΠΊΡΠΈΠ½ΠΎΠΌΡΡΠ΅ΡΡΠ² ΡΠΈΠ½ΡΠ΅Π·ΡΠ²Π°Π»ΠΈ Π»Π°ΠΊΠ°Π·ΠΈ.ΠΠΈΡΠ½ΠΎΠ²ΠΊΠΈ. ΠΡΡΠ½Π΅Π½ΠΎ Π·Π΄Π°ΡΠ½ΡΡΡΡ Π°ΠΊΡΠΈΠ½ΠΎΠΌΡΡΠ΅ΡΡΠ² ΡΠΈΠ·ΠΎΡΡΠ΅ΡΠΈ Helianthemum stevenii Rupr. Ex Juz. & Pozd ΡΠΈΠ½ΡΠ΅Π·ΡΠ²Π°ΡΠΈ Π°Π½ΡΠΈΠΌΡΠΊΡΠΎΠ±Π½Ρ ΡΠ΅ΡΠΎΠ²ΠΈΠ½ΠΈ, ΡΠ΅ΡΠΌΠ΅Π½ΡΠΈ, Π° ΡΠ°ΠΊΠΎΠΆ ΡΠΏΠΎΠ»ΡΠΊΠΈ, Π·Π΄Π°ΡΠ½Ρ ΡΠΏΡΠΈΡΡΠΈ ΡΠΎΡΡΡ Ρ ΡΠΎΠ·Π²ΠΈΡΠΊΡ ΡΠΎΡΠ»ΠΈΠ½. ΠΠΈΠ΄ΡΠ»Π΅Π½ΠΎ ΡΡΠ°ΠΌΠΈ Π· ΡΠΈΡΠΎΠΊΠΈΠΌ ΡΠΏΠ΅ΠΊΡΡΠΎΠΌ Π°Π½ΡΠΈΠΌΡΠΊΡΠΎΠ±Π½ΠΎΡ Π΄ΡΡ, Π° ΡΠ°ΠΊΠΎΠΆ ΡΡΠ°ΠΌΠΈ, ΡΠΊΡ ΡΠΏΠ΅ΡΠΈΡΡΡΠ½ΠΎ ΠΏΡΠΈΠ³Π½ΡΡΡΠ²Π°Π»ΠΈ ΡΡΡΡ ΠΏΠ΅Π²Π½ΠΎΠ³ΠΎ ΡΡΠ°ΠΌΡ ΠΏΠ°ΡΠΎΠ³Π΅Π½Π½ΠΈΡ
ΠΌΡΠΊΡΠΎΠΎΡΠ³Π°Π½ΡΠ·ΠΌΡΠ². ΠΠ»Ρ Π½ΠΈΠ·ΠΊΠΈ Π°Π½ΡΠ°Π³ΠΎΠ½ΡΡΡΡΠ² ΡΡΡΠΎΒΠΏΠ°ΡΠΎΠ³Π΅Π½ΒΠ½ΠΎΡ ΠΌΡΠΊΡΠΎΡΠ»ΠΎΡΠΈ Π±ΡΠ»Π° ΠΏΡΠΈΡΠ°ΠΌΠ°Π½Π½Π° ΡΡΡΠΎΡΡΠΈΠΌΡΠ»ΡΠ²Π°Π»ΡΠ½Π° Π΄ΡΡ. ΠΠΈΠ΄ΡΠ»Π΅Π½ΠΎ ΠΏΡΠΎΠ΄ΡΡΠ΅Π½ΡΡΠ² Π³ΡΠ΄ΡΠΎΠ»Π°Π· ΡΠ° ΠΎΠΊΡΠΈΠ΄ΠΎΡΠ΅Π΄ΡΠΊΡΠ°Π·
Furaquinocins K and L: Novel Naphthoquinone-Based Meroterpenoids from Streptomyces sp. Je 1-369
Actinomycetes are the most prominent group of microorganisms that produce biologically active compounds. Among them, special attention is focused on bacteria in the genus Streptomyces. Streptomycetes are an important source of biologically active natural compounds that could be considered therapeutic agents. In this study, we described the identification, purification, and structure elucidation of two new naphthoquinone-based meroterpenoids, furaquinocins K and L, from Streptomyces sp. Je 1-369 strain, which was isolated from the rhizosphere soil of Juniperus excelsa (Bieb.). The main difference between furaquinocins K and L and the described furaquinocins was a modification in the polyketide naphthoquinone skeleton. In addition, the structure of furaquinocin L contained an acetylhydrazone fragment, which is quite rare for natural compounds. We also identified a furaquinocin biosynthetic gene cluster in the Je 1-369 strain, which showed similarity (60%) with the furaquinocin B biosynthetic gene cluster from Streptomyces sp. KO-3988. Furaquinocin L showed activity against Gram-positive bacteria without cytotoxic effects
The diversity and antibacterial activity of culturable actinobacteria isolated from the rhizosphere soil of Deschampsia antarctica (Galindez Island, Maritime Antarctic)
Antarctic actinobacteria, which can be isolated from both soils and marine sediments, demonstrate a wide range of antimicrobial activities as well as significant biosynthetic potential as the producers of biologically active compounds. However, the actinobacterial diversity of the Antarctic region has not yet been sufficiently studied. The present study sought to examine the diversity and antibacterial activity of culturable actinobacteria isolated from the rhizosphere soil of Deschampsia antarctica (Γ. Desv.), which was collected from Galindez Island, Maritime Antarctic. Among the actinobacteria isolated using a 16S rRNA gene sequence-based phylogenetic analysis process, five genera, namely Streptomyces, Micromonospora, Umezawaea, Kribbella and Micrococcus, were identified. To the best of our knowledge, this is the first report to describe the isolation and initial characterisation of members of the genus Umezawaea from the Antarctic. The isolated actinobacteria were assayed to determine their activity against Gram-positive bacteria, Gram-negative bacteria and yeast. Among the isolated strains, only 30.2% were able to inhibit the growth of at least one of the tested pathogens. The polymerase chain reaction-based screening of the biosynthetic genes revealed the presence of type I polyketide synthases (65.1%), type II polyketide synthases (25.6%) and non-ribosomal peptide synthetases (9.3%) in the actinobacteria strains. The examination of the sensitivity/resistance to antibiotics profile of the actinobacteria strains revealed their high sensitivity in relation to the tested antibiotics. Taken together, the results showed that Antarctic actinobacteria demonstrate potential as the producers of natural bioactive compounds, which means that they represent a valuable prospect for further studies.Ministry of Education and Science of Ukrain
The adpA-like regulatory gene from Actinoplanes teichomyceticus: in silico analysis and heterologous expression
Ostash B, Yushchuk O, Tistechok S, et al. The adpA-like regulatory gene from Actinoplanes teichomyceticus: in silico analysis and heterologous expression. World Journal of Microbiology and Biotechnology. 2015;31(8):1297-1301.Analysis of the draft sequence of the genome of teicoplanin producer Actinoplanes teichomyceticus (NRRL-B16726) led to identification of several genes encoding AraC-family regulators that resemble AdpA, master regulator of transcription in Streptomyces. We elucidated possible regulatory functions of one of the identified genes, adpA19 (at) , most similar to archetypal adpA from model Streptomyces species, in a series of expression experiments. Introduction of adpA19 (at) under control of its own promoter on moderate copy number vector pKC1139 into NRRL-B16726 had no influence on antibiotic production and sporulation. Introduction of adpA19 (at) into Streptomyces coelicolor M145 and several S. ghanaensis strains had major influence on antibiotic production by these bacteria. Finally, adpA19 (at) expression in a set of soil actinomycete isolates led to induction of synthesis of antibiotic compounds. Our data point to pleiotropic regulatory role of adpA19 (at) , warranting its use as a tool to manipulate secondary metabolome of actinomycetes