Statistical modelling for prediction of axis-switching in rectangular jets

Rectangular nozzles are increasingly used for modern military aircraft propulsion installations, including the roll nozzles on the F-35B vertical/short take-off and landing strike fighter. A peculiar phenomenon known as axis-switching is generally observed in such non-axisymmetric nozzle flows during which the jet spreads faster along the minor axis compared to the major axis. This might affect the under-wing stores and aircraft structure. A computational fluid dynamics study was performed to understand the effects of changing the upstream nozzle geometry on a rectangular free jet. A method is proposed, involving the formulation of an equation based upon a statistical model for a rectangular nozzle with an exit aspect ratio (ARe) of 4; the variables under consideration (for a constant nozzle pressure ratio (NPR)) being inlet aspect ratio (ARi) and length of the contraction section. The jet development was characterised using two parameters: location of the cross-over point (Xc) and the difference in the jet half-velocity widths along the major and minor axes (ΔB30). Based on the observed results, two statistical models were formulated for the prediction of axis-switching; the first model gives the location of the cross-over point, while the second model indicates the occurrence of axis-switching for the given configuration

Effects of upstream nozzle geometry on rectangular free jets

This study is aimed at understanding the effects of changing the upstream nozzle geometry on the development of rectangular free jets. An existing converging rectangular nozzle with an exit aspect ratio of 4 and a circular inlet (AR4 nozzle) has been used as the basic configuration for this work. The study is primarily based on the results of numerical simulations wherein the internal geometry variation is accomplished by changing the inlet aspect ratio (AR,) and the length of the converging section, expressed as a ratio with respect to the length of the nozzle (called 'converging section ratio*, CSR); all the other parameters are kept constant. The results from LDA experiments done on the AR4 nozzle are presented and used as validation data for the CPD simulations. Analyses of the numerical results help in understanding the variation of the jet spreading for different combinations of AR, and CSR. Two parameters are identified for describing the jet development: the cross-over point (XC), defined as the location downstream of the exit where the jet half-velocity-widths (B) along the major and minor axes are equal, and the difference in the half-velocity-widths at 30 nozzle equivalent diameters (Dm) from the exit (AB30), to ascertain the occurrence of axis-switching. For a given AR, XC varies linearly with CSR; the variation of XC is non-linear with AR, for a constant CSR. The A1330 variation is non-linear with both AR, and CSR; the other variable being kept constant. The data obtained from the simulations are further used to propose two parametric models which can be used to predict the occurrence of axis-switching, within the scope of this work. The parametric models are validated and future work is proposed.EThOS - Electronic Theses Online ServiceGBUnited Kingdo

Identification of new participants in the rainbow trout (Oncorhynchus mykiss) oocyte maturation and ovulation processes using cDNA microarrays

BACKGROUND: The hormonal control of oocyte maturation and ovulation as well as the molecular mechanisms of nuclear maturation have been thoroughly studied in fish. In contrast, the other molecular events occurring in the ovary during post-vitellogenesis have received far less attention. METHODS: Nylon microarrays displaying 9152 rainbow trout cDNAs were hybridized using RNA samples originating from ovarian tissue collected during late vitellogenesis, post-vitellogenesis and oocyte maturation. Differentially expressed genes were identified using a statistical analysis. A supervised clustering analysis was performed using only differentially expressed genes in order to identify gene clusters exhibiting similar expression profiles. In addition, specific genes were selected and their preovulatory ovarian expression was analyzed using real-time PCR. RESULTS: From the statistical analysis, 310 differentially expressed genes were identified. Among those genes, 90 were up-regulated at the time of oocyte maturation while 220 exhibited an opposite pattern. After clustering analysis, 90 clones belonging to 3 gene clusters exhibiting the most remarkable expression patterns were kept for further analysis. Using real-time PCR analysis, we observed a strong up-regulation of ion and water transport genes such as aquaporin 4 (aqp4) and pendrin (slc26). In addition, a dramatic up-regulation of vasotocin (avt) gene was observed. Furthermore, angiotensin-converting-enzyme 2 (ace2), coagulation factor V (cf5), adam 22, and the chemokine cxcl14 genes exhibited a sharp up-regulation at the time of oocyte maturation. Finally, ovarian aromatase (cyp19a1) exhibited a dramatic down-regulation over the post-vitellogenic period while a down-regulation of Cytidine monophosphate-N-acetylneuraminic acid hydroxylase (cmah) was observed at the time of oocyte maturation. CONCLUSION: We showed the over or under expression of more that 300 genes, most of them being previously unstudied or unknown in the fish preovulatory ovary. Our data confirmed the down-regulation of estrogen synthesis genes during the preovulatory period. In addition, the strong up-regulation of aqp4 and slc26 genes prior to ovulation suggests their participation in the oocyte hydration process occurring at that time. Furthermore, among the most up-regulated clones, several genes such as cxcl14, ace2, adam22, cf5 have pro-inflammatory, vasodilatory, proteolytics and coagulatory functions. The identity and expression patterns of those genes support the theory comparing ovulation to an inflammatory-like reaction

Comparative study: the effect of annealing conditions on the properties of P3HT:PCBM blends

This paper presents a detailed study on the role of various annealing treatments on organic poly(3-hexylthiophene) and [6]-phenyl-C61-butyric acid methyl ester blends under different experimental conditions. A combination of analytical tools is used to study the alteration of the phase separation, structure and photovoltaic properties of the P3HT:PCBM blend during the annealing process. Results showed that the thermal annealing yields PCBM ‘‘needle-like’’ crystals and that prolonged heat treatment leads to extensive phase separation, as demonstrated by the growth in the size and quantity of PCBM crystals. The substrate annealing method demonstrated an optimal morphology by eradicating and suppressing the formation of fullerene clusters across the film, resulting in longer P3HT fibrils with smaller diameter. Improved optical constants, PL quenching and a decrease in the P3HT optical bad-gap were demonstrated for the substrate annealed films due to the limited diffusion of the PCBM molecules. An effective strategy for determining an optimized morphology through substrate annealing treatment is therefore revealed for improved device efficiency.Web of Scienc

ACE2-Mediated Reduction of Oxidative Stress in the Central Nervous System Is Associated with Improvement of Autonomic Function

Oxidative stress in the central nervous system mediates the increase in sympathetic tone that precedes the development of hypertension. We hypothesized that by transforming Angiotensin-II (AngII) into Ang-(1–7), ACE2 might reduce AngII-mediated oxidative stress in the brain and prevent autonomic dysfunction. To test this hypothesis, a relationship between ACE2 and oxidative stress was first confirmed in a mouse neuroblastoma cell line (Neuro2A cells) treated with AngII and infected with Ad-hACE2. ACE2 overexpression resulted in a reduction of reactive oxygen species (ROS) formation. In vivo, ACE2 knockout (ACE2−/y) mice and non-transgenic (NT) littermates were infused with AngII (10 days) and infected with Ad-hACE2 in the paraventricular nucleus (PVN). Baseline blood pressure (BP), AngII and brain ROS levels were not different between young mice (12 weeks). However, cardiac sympathetic tone, brain NADPH oxidase and SOD activities were significantly increased in ACE2−/y. Post infusion, plasma and brain AngII levels were also significantly higher in ACE2−/y, although BP was similarly increased in both genotypes. ROS formation in the PVN and RVLM was significantly higher in ACE2−/y mice following AngII infusion. Similar phenotypes, i.e. increased oxidative stress, exacerbated dysautonomia and hypertension, were also observed on baseline in mature ACE2−/y mice (48 weeks). ACE2 gene therapy to the PVN reduced AngII-mediated increase in NADPH oxidase activity and normalized cardiac dysautonomia in ACE2−/y mice. Altogether, these data indicate that ACE2 gene deletion promotes age-dependent oxidative stress, autonomic dysfunction and hypertension, while PVN-targeted ACE2 gene therapy decreases ROS formation via NADPH oxidase inhibition and improves autonomic function. Accordingly, ACE2 could represent a new target for the treatment of hypertension-associated dysautonomia and oxidative stress

Growth-inhibitory effects of the chemopreventive agent indole-3-carbinol are increased in combination with the polyamine putrescine in the SW480 colon tumour cell line

BACKGROUND: Many tumours undergo disregulation of polyamine homeostasis and upregulation of ornithine decarboxylase (ODC) activity, which can promote carcinogenesis. In animal models of colon carcinogenesis, inhibition of ODC activity by difluoromethylornithine (DFMO) has been shown to reduce the number and size of colon adenomas and carcinomas. Indole-3-carbinol (I3C) has shown promising chemopreventive activity against a range of human tumour cell types, but little is known about the effect of this agent on colon cell lines. Here, we investigated whether inhibition of ODC by I3C could contribute to a chemopreventive effect in colon cell lines. METHODS: Cell cycle progression and induction of apoptosis were assessed by flow cytometry. Ornithine decarboxylase activity was determined by liberation of CO(2 )from (14)C-labelled substrate, and polyamine levels were measured by HPLC. RESULTS: I3C inhibited proliferation of the human colon tumour cell lines HT29 and SW480, and of the normal tissue-derived HCEC line, and at higher concentrations induced apoptosis in SW480 cells. The agent also caused a decrease in ODC activity in a dose-dependent manner. While administration of exogenous putrescine reversed the growth-inhibitory effect of DFMO, it did not reverse the growth-inhibition following an I3C treatment, and in the case of the SW480 cell line, the effect was actually enhanced. In this cell line, combination treatment caused a slight increase in the proportion of cells in the G(2)/M phase of the cell cycle, and increased the proportion of cells undergoing necrosis, but did not predispose cells to apoptosis. Indole-3-carbinol also caused an increase in intracellular spermine levels, which was not modulated by putrescine co-administration. CONCLUSION: While indole-3-carbinol decreased ornithine decarboxylase activity in the colon cell lines, it appears unlikely that this constitutes a major mechanism by which the agent exerts its antiproliferative effect, although accumulation of spermine may cause cytotoxicity and contribute to cell death. The precise mechanism by which putrescine enhances the growth inhibitory effect of the agent remains to be elucidated, but does result in cells undergoing necrosis, possibly following accumulation in the G(2)/M phase of the cell cycle

Apnea of prematurity: from cause to treatment

Apnea of prematurity (AOP) is a common problem affecting premature infants, likely secondary to a “physiologic” immaturity of respiratory control that may be exacerbated by neonatal disease. These include altered ventilatory responses to hypoxia, hypercapnia, and altered sleep states, while the roles of gastroesophageal reflux and anemia remain controversial. Standard clinical management of the obstructive subtype of AOP includes prone positioning and continuous positive or nasal intermittent positive pressure ventilation to prevent pharyngeal collapse and alveolar atelectasis, while methylxanthine therapy is a mainstay of treatment of central apnea by stimulating the central nervous system and respiratory muscle function. Other therapies, including kangaroo care, red blood cell transfusions, and CO2 inhalation, require further study. The physiology and pathophysiology behind AOP are discussed, including the laryngeal chemoreflex and sensitivity to inhibitory neurotransmitters, as are the mechanisms by which different therapies may work and the potential long-term neurodevelopmental consequences of AOP and its treatment

Effects of upstream nozzle geometry on rectangular free jets

This study is aimed at understanding the effects of changing the upstream nozzle geometry on the development of rectangular free jets. An existing converging rectangular nozzle with an exit aspect ratio of 4 and a circular inlet (AR4 nozzle) has been used as the basic configuration for this work. The study is primarily based on the results of numerical simulations wherein the internal geometry variation is accomplished by changing the inlet aspect ratio (AR,) and the length of the converging section, expressed as a ratio with respect to the length of the nozzle (called 'converging section ratio*, CSR); all the other parameters are kept constant. The results from LDA experiments done on the AR4 nozzle are presented and used as validation data for the CPD simulations. Analyses of the numerical results help in understanding the variation of the jet spreading for different combinations of AR, and CSR. Two parameters are identified for describing the jet development: the cross-over point (XC), defined as the location downstream of the exit where the jet half-velocity-widths (B) along the major and minor axes are equal, and the difference in the half-velocity-widths at 30 nozzle equivalent diameters (Dm) from the exit (AB30), to ascertain the occurrence of axis-switching. For a given AR,, XC varies linearly with CSR; the variation of XC is non-linear with AR, for a constant CSR. The A1330 variation is non-linear with both AR, and CSR; the other variable being kept constant. The data obtained from the simulations are further used to propose two parametric models which can be used to predict the occurrence of axis-switching, within the scope of this work. The parametric models are validated and future work is proposed