Strategi Pengembangan Ekowisata Mangrove di Desa Nira Nusa, Nusa Tenggara Timur Menggunakan Analisis Swot

Mangrove Nira Nusa merupakan mangrove yang terletak di Desa Nira Nusa, Kecamatan Maurole, Kabupaten Ende, Flores, NTT. Penelitian ini bertujuan untuk mengetahui potensi dan sumber daya yang tersedia diantaranya ; flora dan fauna , potensi sumber daya alam, potensi sumber daya manusia , dan potensi sosial-budaya di Desa Nira Nusa. Penelitian ini menggunakan metode kualitatif deskriptif. Proses pengambilan data menggunakan observasi lapangan, wawancara, pengisian kuesioner, dan dokumentasi. Hasil penelitian dianalisis menggunakan analisis SWOT untuk menghasilkan strategi S-O yaitu potensi sumber daya mangrove dikembangkan menjadi wisata berbasis edukasi dengan kegiatan seperti pengamatan biota/satwa dan kegiatan memancing. Strategi S-T yaitu memberikan pelatihan khusus tentang konservasi mangrove kepada pengelola dan pemerintah setempat tentang dampak membuang sampah di laut. Strategi W-O yaitu memanfaatkan tingginya minat wisatawan untuk memaksimalkan perbaikan sarana-prasarana. Strategi W-T yaitu menyelenggarakan kegiatan pembersihan lingkungan sekitar mangrove oleh pengelola, masyarakat dan lembaga pemerintahan, membuat aturan tidak menebang pohon di area hutan mangrove

SV channels dominate the vacuolar Ca2+ release during intracellular signaling

AbstractVacuoles have long been suggested to mediate a rise in the cytosolic free Ca2+ during environmental signal transduction. This study addresses the issue of the control of vacuolar calcium release by some of the known signaling molecules such as IP3, cADPR, ABA, ATP, cAMP, cGMP, H2O2 and CaM. Over 30 concentrations and/or combinations of these signaling compounds were studied in a series of electrophysiological experiments involving non-invasive ion flux measurements (the MIFE) and patch-clamp techniques. Our results suggest that calcium, calmodulin and nucleotides cause calcium release via SV channels

Point-of-care haemoglobin accuracy and transfusion outcomes in non-cardiac surgery at a Canadian tertiary academic hospital: protocol for the PREMISE observational study

Introduction Transfusions in surgery can be life-saving interventions, but inappropriate transfusions may lack clinical benefit and cause harm. Transfusion decision-making in surgery is complex and frequently informed by haemoglobin (Hgb) measurement in the operating room. Point-of-care testing for haemoglobin (POCT-Hgb) is increasingly relied on given its simplicity and rapid provision of results. POCT-Hgb devices lack adequate validation in the operative setting, particularly for Hgb values within the transfusion zone (60–100 g/L). This study aims to examine the accuracy of intraoperative POCT-Hgb instruments in non-cardiac surgery, and the association between POCT-Hgb measurements and transfusion decision-making.Methods and analysis PREMISE is an observational prospective method comparison study. Enrolment will occur when adult patients undergoing major non-cardiac surgery require POCT-Hgb, as determined by the treating team. Three concurrent POCT-Hgb results, considered as index tests, will be compared with a laboratory analysis of Hgb (lab-Hgb), considered the gold standard. Participants may have multiple POCT-Hgb measurements during surgery. The primary outcome is the difference in individual Hgb measurements between POCT-Hgb and lab-Hgb, primarily among measurements that are within the transfusion zone. Secondary outcomes include POCT-Hgb accuracy within the entire cohort, postoperative morbidity, mortality and transfusion rates. The sample size is 1750 POCT-Hgb measurements to obtain a minimum of 652 Hgb measurements <100 g/L, based on an estimated incidence of 38%. The sample size was calculated to fit a logistic regression model to predict instances when POCT-Hgb are inaccurate, using 4 g/L as an acceptable margin of error.Ethics and dissemination Institutional ethics approval has been obtained by the Ottawa Health Science Network—Research Ethics Board prior to initiating the study. Findings from this study will be published in peer-reviewed journals and presented at relevant scientific conferences. Social media will be leveraged to further disseminate the study results and engage with clinicians

Phlebotomy resulting in controlled hypovolemia to prevent blood loss in major hepatic resections (PRICE-2) : study protocol for a phase 3 randomized controlled trial

Introduction: Blood loss and red blood cell (RBC) transfusion in liver surgery are areas of concern for surgeons, anesthesiologists, and patients alike. While various methods are employed to reduce surgical blood loss, the evidence base surrounding each intervention is limited. Hypovolemic phlebotomy, the removal of whole blood from the patient without volume replacement during liver transection, has been strongly associated with decreased bleeding and RBC transfusion in observational studies. This trial aims to investigate whether hypovolemic phlebotomy is superior to usual care in reducing RBC transfusions in liver resection. Methods: This study is a double-blind multicenter randomized controlled trial. Adult patients undergoing major hepatic resections for any indication will be randomly allocated in a 1:1 ratio to either hypovolemic phlebotomy and usual care or usual care alone. Exclusion criteria will be minor resections, preoperative hemoglobin <100g/L, renal insufficiency, and other contraindication to hypovolemic phlebotomy. The primary outcome will be the proportion of patients receiving at least one allogeneic RBC transfusion unit within 30 days of the onset of surgery. Secondary outcomes will include transfusion of other allogeneic blood products, blood loss, morbidity, mortality, and intraoperative physiologic parameters. The surgical team will be blinded to the intervention. Randomization will occur on the morning of surgery. The sample size will comprise 440 patients. Enrolment will occur at four Canadian academic liver surgery centers over a 4-year period. Ethics approval will be obtained at participating sites before enrolment. Discussion: The results of this randomized control trial will provide high-quality evidence regarding the use of hypovolemic phlebotomy in major liver resection and its effects on RBC transfusion. If proven to be effective, this intervention could become standard of care in liver operations internationally and become incorporated within perioperative patient blood management programs.Medicine, Faculty ofNon UBCAnesthesiology, Pharmacology and Therapeutics, Department ofSurgery, Department ofReviewedFacultyResearcherPostdoctoralOthe