Additional file 1: of DNA-methylation in C1R is a prognostic biomarker for acute myeloid leukemia

PDF with all supplemental data. This file contains additional details on methods, Tables S1–S7, and Figures S1–S10. (PDF 1718 kb

Simultaneous determination of glutathione, glutathione disulphide, paracetamol and its sulphur containing metabolites using HPLC and electrochemical detection with on-line generated bromine

A Viability of hMSC of all sources during a 7-day follow-up period during early passages (passages 3–5) in culture (results expressed as arbitrary units of normalized fluorescence). Black depicts CV-MSC (n = 3), dark gray UC-MSC (n = 3), medium gray AT-MSC (n = 3), and light gray BM-MSC (n = 3). B Histograms for CV-MSC in passage 9 (n = 3) and UC-MSC in passage 4 (n = 3) stained for β-galactosidase assessed by flow cytometry. C Visualization of collagen contraction potential by CV-MSC (i), BM-MSC (ii), UC-MSC (iii), and AT-MSC (iv). All donors shown. Scale = 1 cm. D Immunofluorescence of early passaged BM-MSC (i, iv), UC-MSC (ii–v), and AT-MSC (iii, vi) stained for SM22α (i–iii) and α-SMA (iv–vi). Scale = 50 μm. All conditions n ≥ 3. (JPG 63 kb

Additional file 3: Figure S3. of Comprehensive characterization of chorionic villi-derived mesenchymal stromal cells from human placenta

A Difference between predicted and chronological MSC donor age (years) after EAS: CV-MSC 37.75 ± 5.43 years (n = 4), BM-MSC –16.00 ± 10.06 years (n = 4), UC-MSC 25.50 ± 1.84 years (n = 4), AT-MSC 17.00 ± 5.00 (n = 2), from passage 2 to passage 5 (**p < 0.005). B Difference between predicted and chronological MSC donor age (years) after EAS: CV-MSC 29.25 ± 4.46 years (n = 4), BM-MSC –26.40 ± 10.52 years (n = 5), AT-MSC 32.80 ± 9.65 (n = 5), from passage 6 to passage 15 (**p < 0.005). It was not possible to keep UC-MSC until late passages. C Predicted age (years) versus passage number EAS: one representative donor shown for CV-MSC (black), UC-MSC (dark gray), AT-MSC (medium gray), and BM-MSC (light gray). (JPG 33 kb

Additional file 1: Figure S1. of Comprehensive characterization of chorionic villi-derived mesenchymal stromal cells from human placenta

A Bright-field microscopy images of cultured UC-MSC in passage 3 (i) and AT-MSC in passage 24 (ii). Scale = 500 μm. B Visualization of calcium deposits after Alizarin Red stain (i–iii, scale = 500 μm), proteogyclans after Toluin Blue stain (iv–vi, scale = 1 mm), and lipid droplets after Oil Red O stain (vii–ix, scale = 100 μm) of differentiated AT-MSC (i, iv, vii), BM-MSC (ii, v, viii), and UC-MSC (iii, vi, ix) all in passage 3. (JPG 86 kb

Analyse des sagittalen lumbosakralen Alignments nach Korrekturspondylodese isthmischer Spondylolisthesen

(A) Representative TEM images of c-kit+ cells used for initiating co-cultures after immunomagnetic bead selection. (B) TEM images of 4- and 14-day cultured c-kit+ cells on 500-μm β-TCP scaffolds, 500-μm β-TCP/collagen I/III scaffolds, and 500-μm β-TCP/Matrigel® scaffolds. Collagen I/III gels and Matrigel® alone are shown as controls. C-kit+ progenitor cell morphology (of freshly isolated cells) consisting of visible scattered chromatin, prominent nucleoli, and approximated cell diameter of 8–10 μm is shown as reference

Additional file 2: Figure S1. of An engineered multicomponent bone marrow niche for the recapitulation of hematopoiesis at ectopic transplantation sites

Representative SEM images of 500- or 800-μm β-TCP scaffolds pre-seeded with hMSCs maintained in culture for 3 weeks with or without addition of collagen I/III. Collagen I/III embedded with hMSCs cultured under standard conditions were undifferentiated controls. Collagen I/III gels with embedded hMSCs cultured under osteogenic differentiation conditions were osteogenic controls (xii)

Additional file 4: Figure S3. of An engineered multicomponent bone marrow niche for the recapitulation of hematopoiesis at ectopic transplantation sites

Dot plot representations of the putative LSK (lin−Sca1+c-kit+) population in starting cultures and 4-, 7-, and 14-day cultures of c-kit+-isolated cells in co-culture with mBMSCs on 500- and 800-μm β-TCP scaffolds with or without collagen I/III gels or Matrigel®. Flow cytometry data shown is of one representative experiment

Additional file 3: Figure S2. of An engineered multicomponent bone marrow niche for the recapitulation of hematopoiesis at ectopic transplantation sites

(A) Monitorization of the CD34+CD38− primitive phenotype in human-derived CD34+ progenitors co-cultured with hMSCs for 5 and 12 days in the different β-TCP/matrix hybrids. On the left data presented is a mean ± SD of three independent experiments; on the right, dot plots of one representative experiment are shown. (B) Representative SEM images CD34+ HSPCs co-cultured for 12 days in 800-μm β-TCP scaffolds in the presence of hMSC-containing collagen I/III matrix. CD34+ HSPCs (red arrows) are seen in close contact to hMSCs (white arrows) within the scaffold macropores. β-TCP scaffolds (asterisks) were reinforced with collagen I/III (green arrows)

Additional file 7: Figure S6. of An engineered multicomponent bone marrow niche for the recapitulation of hematopoiesis at ectopic transplantation sites

Representative SEM images of collagen I/III gels pre-seeded with mBMSCs and later seeded with c-kit+-isolated cells. Co-cultures observed were maintained for 4 days (vi) and 14 days (i, iv, v). SEM in cryogenic mode (ii) and dry mode (iii) shows the typically high microporosity rate of collagen I/III