Hypoxia-Inducible Factor-1α Regulates CD55 in Airway Epithelium

Airway epithelial CD55 down-regulation occurs in several hypoxia-associated pulmonary diseases, but the mechanism is unknown. Using in vivo and in vitro assays of pharmacologic inhibition and gene silencing, the current study investigated the role of hypoxia-inducible factor (HIF)-1α in regulating airway epithelial CD55 expression. Hypoxia down-regulated CD55 expression on small-airway epithelial cells in vitro, and in murine lungs in vivo; the latter was associated with local complement activation. Treatment with pharmacologic inhibition or silencing of HIF-1α during hypoxia-recovered CD55 expression in small-airway epithelial cells. HIF-1α overexpression or blockade, in vitro or in vivo, down-regulated CD55 expression. Collectively, these data show a key role for HIF-1α in regulating the expression of CD55 on airway epithelium

Committee autonomy in parliamentary systems – coalition logic or congressional rationales?

The Institutions of Politics; Design, Workings, and implications ( do not use, ended 1-1-2020

Not so random after all? – revisiting committee assignments in Dáil Éireann

In this paper, I analyse the allocation of legislators to specialised committees in Dáil Éireann. Committees are privileged decision-making arenas: Committee members have, once assigned, the right and duty to work on issues within their jurisdiction. It is, therefore, important to understand which factors influence the assignment process. In what has been the most thorough analysis hitherto, Hansen [(2011). A random process? Committee assignments in Dail Eireann. Irish Political Studies, 26(3), 345–360] has found very little patterns explaining this process in the Dáil. The study uses the congressional theories of legislative organisation as heuristic devices to deduce several rationales of the assignment process. The role of parliamentary party groups in the assignment process is highlighted. The hypotheses are tested by means of a multiple-membership multilevel model on committee assignments across multiple legislative periods (1992–2011). The results are backed up with evidence from 22 interviews I conducted with Irish legislators. Contradicting earlier assessments of the assignment process, several factors increase the likelihood of being assigned to a committee. There is strong evidence for a reassignment pattern. Additionally, a legislator's occupational background matters in the assignment process. For the ongoing discussion about the applicability of the congressional theories outside of the U.S., my study provides evidence for the usefulness of the U.S. theories.The Institutions of Politics; Design, Workings, and implications ( do not use, ended 1-1-2020

Legislative Bellwethers: The Role of Committee Membership in Parliamentary Debate

Political parties and legislators use legislative debates to establish their reputation, challenge rivals, and engage in coalition management, among many other tasks. Yet, existing theories on parliamentary debates have abstracted away from the need for information and expertise, which are costly to acquire. Drawing on the “informational” perspective on legislative organization, we address this problem by arguing that party leaders use committees as training arenas for their backbenchers. They task their assigned members with acquiring specific expertise and then rely heavily on those members during the corresponding debates. We turn to the Portuguese legislature, from 2000 to 2015, to discuss how saliency, government dynamics, and party size affect the use of experts. We test this theory using a novel approach to classify speeches that leverages the texts of legislation as training data for a supervised approach.info:eu-repo/semantics/publishedVersio

Transcriptomic modifications in developmental cardiopulmonary adaptations to chronic hypoxia using a murine model of simulated high-altitude exposure

Mechanisms driving adaptive developmental responses to chronic high-altitude (HA) exposure are incompletely known. We developed a novel rat model mimicking the human condition of cardiopulmonary adaptation to HA starting at conception and spanning the in utero and postnatal timeframe. We assessed lung growth and cardiopulmonary structure and function and performed transcriptome analyses to identify mechanisms facilitating developmental adaptations to chronic hypoxia. To generate the model, breeding pairs of Sprague-Dawley rats were exposed to hypobaric hypoxia (equivalent to 9,000 ft elevation). Mating, pregnancy, and delivery occurred in hypoxic conditions. Six weeks postpartum, structural and functional data were collected in the offspring. RNA-Seq was performed on right ventricle (RV) and lung tissue. Age-matched breeding pairs and offspring under room air (RA) conditions served as controls. Hypoxic rats exhibited significantly lower body weights and higher hematocrit levels, alveolar volumes, pulmonary diffusion capacities, RV mass, and RV systolic pressure, as well as increased pulmonary artery remodeling. RNA-Seq analyses revealed multiple differentially expressed genes in lungs and RVs from hypoxic rats. Although there was considerable similarity between hypoxic lungs and RVs compared with RA controls, several upstream regulators unique to lung or RV were identified. We noted a pattern of immune downregulation and regulation patterns of immune and hormonal mediators similar to the genome from patients with pulmonary arterial hypertension. In summary, we developed a novel murine model of chronic hypoxia exposure that demonstrates functional and structural phenotypes similar to human adaptation. We identified transcriptomic alterations that suggest potential mechanisms for adaptation to chronic HA

Who gets what and why? Committee assignments in the German Bundestag

Although committee assignments have been researched in various parliaments, findings are inconclusive. This article contributes to the debate by analysing the allocations to specialised committees in the Bundestag, a legislature that establishes strong committees despite having strong parliamentary party groups. Studying assignments in this legislature can, therefore, help us to solve the present disjunction between ‘European’ and ‘American’ experiences regarding committee research. The study uses the congressional theories of legislative organisation as heuristic devices but explicitly highlights the strong involvement of parliamentary party groups. The hypotheses are tested with a multiple membership multilevel model across several legislative periods (1990‒2013) and backed up with evidence from 51 interviews with German legislators. The results show that next to constituency demands and the influence of regional factions, there is strong evidence for a reassignment pattern and that a legislator’s occupational background and connections to interest groups matter in the assignment process.The Institutions of Politics; Design, Workings, and implications ( do not use, ended 1-1-2020